Srgap3 (NM_001191975) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1563152
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217473 representing NM_001191975 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTCAAACTAAGTTCAAGAAAGACAAAGAGATCATTGCTGAATATGAAGCCCAAATAAAAGAGA TCCGCACGCAGCTGGTGGAGCAGTTCAAATGCCTGGAGCAGCAGTCAGAGTCGCGGCTGCAGCTGCTGCA AGACTTGCAGGAGTTCTTCCGCAGGAAAGCTGAGATCGAGCTGGAGTATTCCCGCAGCCTGGAGAAGCTG GCCGAGCGCTTCTCCTCCAAGATTCGAAGCTCCCGGGAGCACCAGTTCAAGAAGGATCAATACCTCCTCT CACCTGTGAACTGTTGGTATCTGGTACTGCATCAGACTCGGCGGGAGAGCCGAGATCACGCCACCCTCAA TGACATCTTCATGAACAATGTCATCGTCCGCCTCTCCCAGATCAGTGAAGATGTCATCAGACTCTTCAAA AAGAGCAAAGAGATTGGCCTGCAGATGCATGAGGAGCTGCTGAAGGTGACCAATGAGCTCTACACAGTCA TGAAAACCTACCACATGTACCATGCAGAGAGCATCAGTGCAGAAAGCAAGCTGAAGGAGGCCGAGAAGCA GGAGGAGAAACAGTTTAACAAGTCAGGAGACCTGAGCATGAACCTTCTCCGGCACGAGGACAGACCCCAG CGCCGCAGCTCCGTGAAGAAGATTGAGAAGATGAAGGAGAAGAGGCAGGCCAAGTATTCTGAGAACAAGC TCAAGTGCACAAAGGCCCGAAATGACTACCTGCTGAATCTGGCAGCCACCAACGCAGCTATCAGCAAATA CTACATCCACGATGTCTCTGATCTCATCGATTGCTGTGATTTGGGCTTTCACGCCAGCCTGGCCCGCACC TTTCGGACCTACCTCTCAGCAGAATATAACCTGGAGACCTCTCGCCACGAGGGCCTGGACATCATAGAGA ATGCAGTGGACAACCTGGACTCCCGGAGTGACAAGCACACAGTCATGGACATGTGCAGCCAGGTCTTCTG CCCTCCACTCAAGTTTGAGTTCCAGCCCCACATGGGGGATGAGGTCTGCCAGGTCAGTGCACAGCAGCCT GTCCAGACGGAGCTGCTTATGCGGTACCACCAGCTGCAGTCCAGGCTGGCCACTCTCAAGATTGAGAACG AAGAGGTTAGGAAAACCCTGGATGCCACCATGCAGACCTTACAGGACATGCTGACCGTGGAGGACTTTGA TGTCTCTGACGCCTTCCAACACAGCCGATCTACAGAGTCCATAAAGTCAGCTGCTTCTGAGACCTACATG AGCAAAATCAACATCGCCAAAAGGAGAGCCAACCAGCAGGAGACAGAAATGTTTTATTTTACAAAATTTA AAGAATATGTGAATGGCAGTAACCTCATCACCAAGCTGCAAGCCAAGCATGACTTACTCAAGCAGACCCT GGGTGAAGGGGAGAGAGCAGAGTGCGGCACAACCAGGCCCCCCTGTCTTCCCCCTAAACCACAGAAAATG AGGAGACCTAGGCCTCTTTCAGTGTATAGCCATAAACTCTTTAACGGCAGTATGGAAGCGTTCATTAAGG ATTCAGGACAAGCTATACCACTTGTAGTCGAGAGCTGTATTCGATTCATCAATTTATATGGACTCCAGCA GCAGGGGATCTTCAGAGTTCCAGGGTCTCAGGTTGAAGTCAATGACATCAAGAATTCCTTTGAGAGAGGT GAAGACCCCCTCGTGGATGACCAAAATGAGCGAGATATCAATTCAGTTGCTGGTGTTTTAAAACTGTATT TCCGAGGACTGGAAAACCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTGATATCTACTATTAAACTGGA GAACCCAGCTGACAGGGTGCACCCAATCCAGCAGATCCTCATCACCCTGCCCCGCGTGGTCATTGTGGTC ATGAGATACCTCTTTGCTTTCCTCAATCACCTTTCCCAGTACAGTGATGAGAACATGATGGATCCCTACA ACTTGGCCATCTGCTTCGGGCCCACCCTCATGCACATCCCTGATGGGCAGGACCCTGTATCCTGCCAGGC ACATGTCAATGAAGTCATCAAAACCATCATCATCCACCATGAAGCCATCTTCCCCAGCCCGAGGGAGCTA GAGGGACCCGTATACGAAAAATGCATGGCTGGAGGGGAAGAGTACTGTGACAGCCCACACAGTGAGCCGG GGACCATTGATGAAGTTGACCATGACAATGGCACGGAGCCTCATACCAGCGATGAAGAAGTGGAACAGAT CGAGGCCATCGCCAAGTTTGACTATGTGGGGCGGTCCCCTCGTGAACTATCTTTCAAAAAAGGGGCCTCA CTACTCCTGTACCACCGTGCCTCCGAGGACTGGTGGGAGGGCCGTCACAATGGCGTAGATGGACTCATCC CTCATCAGTACATAGTTGTACAGGACATGGATGATGCCTTCTCCGATAGCCTGAGCCAAAAGGCTGACAG CGAGGCCAGCAGTGGGCCACTGCTGGATGACAAGGCCTCCTCCAAGAATGACCTCCAGTCCCCCACAGAG CACATCTCTGATTACGGCTTTGGGGGGGTGATGGGCCGAGTGAGACTACGGTCCGACGGAGCAGCCATCC CCAGGCGCCGAAGTGGGGGCGACACACACAGCCCACCCCGGGGCTTGGGTCCCAGCATAGATACGCCACC CCGAGCTGCTGCCTGTCCCAGCAGCCCCCACAAAATCCCCCTCAGCCGGGGACGGATTGAGAGCCCTGAG AAGAGGAGGATGGCGACGTTCGGAAGCGCTGGCAGCATCAACTACCCTGACAAGAAGGCGCTGACAGAAG GGCTCTCCATGAGGTCAACTTGCGGCTCCACTCGACACAGCAGCCTAGGGGACCACAAGTCCCTAGAGGC TGAGGCCCTCGCAGAAGACATCGAGAAGACCATGAGCACGGCTCTTCATGAGTTGCGGGAACTTGAGAGA CAGAACACTGTCAAGCAGGCACCAGATGTGGTGCTGGACACCCTGGAGCCCCTAAAGAATCCACCAGGCC CCATCAGCTCAGAGCCTGCCAGCCCCCTGCACACCATTGTCATCCGAGACCCCGATGCTGCCATGCGTCG AAGCAGCAGCTCGTCTACAGAGATGATGACCACCTTCAAGCCAGCCCTGTCGGCTCGCCTGGCTGGTGCC CAGCTGCGTCCACCCCCCATGCGTCCAGTCAGACCTGTGGTCCAGCACCGGTCCAGCAGCAGCAGCAGCT CAGGTGTGGGCAGCCCGGCAGTGACACCTACCGAGAAGATGTTCCCTAATAGCTCCTCAGACAAGTCCGG CACTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217473 representing NM_001191975 Red=Cloning site Green=Tags(s) MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKL AERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFK KSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQ RRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLART FRTYLSAEYNLETSRHEGLDIIENAVDNLDSRSDKHTVMDMCSQVFCPPLKFEFQPHMGDEVCQVSAQQP VQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESIKSAASETYM SKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPPKPQKM RRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRFINLYGLQQQGIFRVPGSQVEVNDIKNSFERG EDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPADRVHPIQQILITLPRVVIVV MRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHVNEVIKTIIIHHEAIFPSPREL EGPVYEKCMAGGEEYCDSPHSEPGTIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYVGRSPRELSFKKGAS LLLYHRASEDWWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTE HISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIPLSRGRIESPE KRRMATFGSAGSINYPDKKALTEGLSMRSTCGSTRHSSLGDHKSLEAEALAEDIEKTMSTALHELRELER QNTVKQAPDVVLDTLEPLKNPPGPISSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGA QLRPPPMRPVRPVVQHRSSSSSSSGVGSPAVTPTEKMFPNSSSDKSGTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191975
ORF Size	3297 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191975.1, NP_001178904.1
RefSeq Size	3671 bp
RefSeq ORF	3300 bp
Locus ID	500287
MW	124.4 kDa

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