Srgap3 (NM_001191975) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1563152
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217473 representing NM_001191975 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTCAAACTAAGTTCAAGAAAGACAAAGAGATCATTGCTGAATATGAAGCCCAAATAAAAGAGA TCCGCACGCAGCTGGTGGAGCAGTTCAAATGCCTGGAGCAGCAGTCAGAGTCGCGGCTGCAGCTGCTGCA AGACTTGCAGGAGTTCTTCCGCAGGAAAGCTGAGATCGAGCTGGAGTATTCCCGCAGCCTGGAGAAGCTG GCCGAGCGCTTCTCCTCCAAGATTCGAAGCTCCCGGGAGCACCAGTTCAAGAAGGATCAATACCTCCTCT CACCTGTGAACTGTTGGTATCTGGTACTGCATCAGACTCGGCGGGAGAGCCGAGATCACGCCACCCTCAA TGACATCTTCATGAACAATGTCATCGTCCGCCTCTCCCAGATCAGTGAAGATGTCATCAGACTCTTCAAA AAGAGCAAAGAGATTGGCCTGCAGATGCATGAGGAGCTGCTGAAGGTGACCAATGAGCTCTACACAGTCA TGAAAACCTACCACATGTACCATGCAGAGAGCATCAGTGCAGAAAGCAAGCTGAAGGAGGCCGAGAAGCA GGAGGAGAAACAGTTTAACAAGTCAGGAGACCTGAGCATGAACCTTCTCCGGCACGAGGACAGACCCCAG CGCCGCAGCTCCGTGAAGAAGATTGAGAAGATGAAGGAGAAGAGGCAGGCCAAGTATTCTGAGAACAAGC TCAAGTGCACAAAGGCCCGAAATGACTACCTGCTGAATCTGGCAGCCACCAACGCAGCTATCAGCAAATA CTACATCCACGATGTCTCTGATCTCATCGATTGCTGTGATTTGGGCTTTCACGCCAGCCTGGCCCGCACC TTTCGGACCTACCTCTCAGCAGAATATAACCTGGAGACCTCTCGCCACGAGGGCCTGGACATCATAGAGA ATGCAGTGGACAACCTGGACTCCCGGAGTGACAAGCACACAGTCATGGACATGTGCAGCCAGGTCTTCTG CCCTCCACTCAAGTTTGAGTTCCAGCCCCACATGGGGGATGAGGTCTGCCAGGTCAGTGCACAGCAGCCT GTCCAGACGGAGCTGCTTATGCGGTACCACCAGCTGCAGTCCAGGCTGGCCACTCTCAAGATTGAGAACG AAGAGGTTAGGAAAACCCTGGATGCCACCATGCAGACCTTACAGGACATGCTGACCGTGGAGGACTTTGA TGTCTCTGACGCCTTCCAACACAGCCGATCTACAGAGTCCATAAAGTCAGCTGCTTCTGAGACCTACATG AGCAAAATCAACATCGCCAAAAGGAGAGCCAACCAGCAGGAGACAGAAATGTTTTATTTTACAAAATTTA AAGAATATGTGAATGGCAGTAACCTCATCACCAAGCTGCAAGCCAAGCATGACTTACTCAAGCAGACCCT GGGTGAAGGGGAGAGAGCAGAGTGCGGCACAACCAGGCCCCCCTGTCTTCCCCCTAAACCACAGAAAATG AGGAGACCTAGGCCTCTTTCAGTGTATAGCCATAAACTCTTTAACGGCAGTATGGAAGCGTTCATTAAGG ATTCAGGACAAGCTATACCACTTGTAGTCGAGAGCTGTATTCGATTCATCAATTTATATGGACTCCAGCA GCAGGGGATCTTCAGAGTTCCAGGGTCTCAGGTTGAAGTCAATGACATCAAGAATTCCTTTGAGAGAGGT GAAGACCCCCTCGTGGATGACCAAAATGAGCGAGATATCAATTCAGTTGCTGGTGTTTTAAAACTGTATT TCCGAGGACTGGAAAACCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTGATATCTACTATTAAACTGGA GAACCCAGCTGACAGGGTGCACCCAATCCAGCAGATCCTCATCACCCTGCCCCGCGTGGTCATTGTGGTC ATGAGATACCTCTTTGCTTTCCTCAATCACCTTTCCCAGTACAGTGATGAGAACATGATGGATCCCTACA ACTTGGCCATCTGCTTCGGGCCCACCCTCATGCACATCCCTGATGGGCAGGACCCTGTATCCTGCCAGGC ACATGTCAATGAAGTCATCAAAACCATCATCATCCACCATGAAGCCATCTTCCCCAGCCCGAGGGAGCTA GAGGGACCCGTATACGAAAAATGCATGGCTGGAGGGGAAGAGTACTGTGACAGCCCACACAGTGAGCCGG GGACCATTGATGAAGTTGACCATGACAATGGCACGGAGCCTCATACCAGCGATGAAGAAGTGGAACAGAT CGAGGCCATCGCCAAGTTTGACTATGTGGGGCGGTCCCCTCGTGAACTATCTTTCAAAAAAGGGGCCTCA CTACTCCTGTACCACCGTGCCTCCGAGGACTGGTGGGAGGGCCGTCACAATGGCGTAGATGGACTCATCC CTCATCAGTACATAGTTGTACAGGACATGGATGATGCCTTCTCCGATAGCCTGAGCCAAAAGGCTGACAG CGAGGCCAGCAGTGGGCCACTGCTGGATGACAAGGCCTCCTCCAAGAATGACCTCCAGTCCCCCACAGAG CACATCTCTGATTACGGCTTTGGGGGGGTGATGGGCCGAGTGAGACTACGGTCCGACGGAGCAGCCATCC CCAGGCGCCGAAGTGGGGGCGACACACACAGCCCACCCCGGGGCTTGGGTCCCAGCATAGATACGCCACC CCGAGCTGCTGCCTGTCCCAGCAGCCCCCACAAAATCCCCCTCAGCCGGGGACGGATTGAGAGCCCTGAG AAGAGGAGGATGGCGACGTTCGGAAGCGCTGGCAGCATCAACTACCCTGACAAGAAGGCGCTGACAGAAG GGCTCTCCATGAGGTCAACTTGCGGCTCCACTCGACACAGCAGCCTAGGGGACCACAAGTCCCTAGAGGC TGAGGCCCTCGCAGAAGACATCGAGAAGACCATGAGCACGGCTCTTCATGAGTTGCGGGAACTTGAGAGA CAGAACACTGTCAAGCAGGCACCAGATGTGGTGCTGGACACCCTGGAGCCCCTAAAGAATCCACCAGGCC CCATCAGCTCAGAGCCTGCCAGCCCCCTGCACACCATTGTCATCCGAGACCCCGATGCTGCCATGCGTCG AAGCAGCAGCTCGTCTACAGAGATGATGACCACCTTCAAGCCAGCCCTGTCGGCTCGCCTGGCTGGTGCC CAGCTGCGTCCACCCCCCATGCGTCCAGTCAGACCTGTGGTCCAGCACCGGTCCAGCAGCAGCAGCAGCT CAGGTGTGGGCAGCCCGGCAGTGACACCTACCGAGAAGATGTTCCCTAATAGCTCCTCAGACAAGTCCGG CACTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191975
Insert Size	3300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191975.1, NP_001178904.1
RefSeq Size	3671 bp
RefSeq ORF	3300 bp
Locus ID	500287

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