Srgap1 (NM_001191784) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1566260
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217466 representing NM_001191784 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCCCGAGCAGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAA TCCGAGCTCAGCTGGTAGAGCAACAAAAATGCCTGGAGCAGCAGACGGAGATGCGAGTTCAGCTGCTCCA GGACCTACAGGATTTCTTCCGGAAGAAAGCCGAAATTGAGACCGAATATTCTCGGAACCTGGAGAAGCTG GCAGAAAGGTTCATGGCCAAAACAAGAAGCACTAAGGACCATCAACAGTTCAAGAAAGACCAGAACCTGT TGTCTCCGGTGAACTGCTGGTATTTGCTCCTGAACCAAGTGAGAAGAGAGAGCAAAGACCACGCCACCTT GAGTGACATCTACCTGAACAACGTCATTATGCGGTTCATGCAGATAAGCGAGGACTCCACCCGGATGTTT AAAAAGAGCAAAGAGATTGCATTCCAGCTGCACGAGGACTTAATGAAGGTTCTTAACGAGCTTTACACGG TGATGAAAACCTACCACATGTACCACTCGGAGAGCATCAGTGCAGAAAGCAAGCTGAAAGAAGCCGAGAA ACAAGAGGAGAAGCAAATGGGGAGATCTGGTGACCCCGTCTTCCACATTAGGCTAGAAGAGAGGCATCAG CGGCGGAGCTCTGTAAAGAAAATTGAAAAAATGAAGGAAAAGAGACAAGCAAAATACTCAGAAAACAAGC TGAAGTCGATCAAAGCACGCAACGAGTATCTCCTAACCCTTGAAGCAACCAACGCCTCTGTGTTCAAGTA TTACATCCACGACCTTTCGGACTTAATCGATTGCTGTGATCTCGGCTACCATGCAAGTCTGAACAGAGCC CTCAGAACATACCTCTCGGCGGAGTACAACCTTGAAACCTCCAGACACGAAGGCTTAGACATCATTGAAA ATGCTGTGGACAATTTAGAGCCAAGAAGTGACAAGCAGAGATTCATGGAGATGTACCCTGCGGCATTCTG CCCACCAATGAAATTTGAGTTTCAGTCTCACATGGGCGATGAGGTGTGTCAGGTCAGCGCCCAGCAGCCC GTCCAGGCGGAACTCATGCTCAGGAACCAACAGCTGCAGTCACGACTGGCCACGCTCAAGATCGAGAGCG AGGAGGTCAAGAAAACAACAGAAGCCACCCTCCAGACGATCCAAGATATGGTCACTATTGAGGACTACGA TGTGTCCGAGTGCTTCCAGCACAGCCGATCCACAGAGTCTGTGAAGTCCACTGTGTCTGAAACCTATCTG AGTAAACCCAGCATCGCCAAGAGACGAGCTAACCAGCAAGAAACTGAGCAGTTCTACTTCATGAAACTTC GAGAGTTCCTGGAAGGCAGTAGCCTCATCACAAAGCTCCAAGCTAAACACGACTTGCTGCAGAGGACTCT TGGAGAAGGTCATAGAGCTGAATACATGACTACAAGGCCTCCAAACGTCCCCCCTAAGCCCCAGAAACAC AGGAAGTCCAGGCCCCGCTCCCAGTATAATGCTAAGTTGTTTAATGGGGATTTGGAGACATTCGTCAAGG ACTCAGGCCAGGTTATCCCTCTCATTGTGGAAAGCTGCATCCGATTCATCAATCTCTATGGTCTTCAGCA TCAGGGGATTTTCAGAGTGTCTGGTTCCCAGGTGGAAGTGAACGATATCAAGAATTCTTTCGAGAGAGGT GAGAATCCTCTGGCCGATGACCAGAGCAACCATGATATTAACTCTGTGGCCGGTGTTCTGAAACTGTACT TCCGTGGGTTGGAAAACCCCCTCTTTCCTAAGGAAAGGTTTAACGACCTCATTTCGTGTATCAGAATAGA TAACCTCTATGAGAGGGCACTCCACATCCACAAACTCCTCCTGACCTTGCCCAGGTCAGTCCTTATAGTG ATGAGGTACCTGTTCGCCTTCCTCAATCACCTCTCCCAGTACAGTGACGAGAACATGATGGACCCTTACA ATCTGGCCATTTGCTTTGGCCCAACACTGATGCCAGTTCCGGAAATACAGGACCAAGTGTCTTGCCAGGC ACACGTGAACGAGATCGTGAAGACGATCATCATTCATCACGAGACTATTTTCCCAGATGCGAAAGAACTG GATGGCCCTGTGTACGAGAAATGTATGGCCGGAGGCGACTACTGCGACAGCCCATACAGTGAGCATGGTA CCCTGGAGGAGGTGGACCAAGACGCTGGCACAGAGCCCCATACCAGTGAAGACGAGTGTGAGCCCATCGA AGCCATAGCCAAGTTTGACTATGTCGGGCGGTCAGCTAGAGAGCTGTCCTTCAAGAAGGGTGCTTCACTG CTGCTGTATCACCGAGCTTCTGAGGACTGGTGGGAAGGCAGACACAACGGCATCGATGGGCTTGTGCCTC ATCAGTACATAGTTGTGCAAGATATGGACGATACATTTTCAGACACGCTGAGCCAAAAAGCCGACAGCGA AGCCAGCAGCGGGCCAGTCCCTGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACTGACCGCCAT TCCGATAGCTATTTAGCCAGACAAAGAAAGAGAGGAGAACCACCCCCTCCGGGAAGACGTCCTGGAAGGA CCAGTGACGGCCATTGTCCCCTGCATCCCCCACATGCTCTGTCTAACTCCTCGATTGATCTGGGATCCCC AAACCTAACCGGTCACCCCAGGGGCCTGTTGCAGAACCGTGGCCTCAACAATGACAGTCCTGAGAGGCGA CGCCGGCCGGGCCATGGCAGCCTGACCAATATCAGCCGCCATGACTCCCTCAAGAAGATAGACAGCCCTC CTATCCGGAGGTCTACGTCATCAGGGCAGTACACCGGCTTCAATGACCACAAGCCGCTAGACCCCGAGAC AATTGCTCAGGATATTGAAGAGACCATGAATACGGCTTTGAACGAACTCCGTGAGCTGGAGAGGCAGAGC ACAGTGAAACACGCCCCGGATGTCGTGCTAGACACCTTGGAGCAAGTGAAAAATTCGCCCACCCCTGCGA CTTCCACAGAATCTCTCAGCCCGCTGCACAGCGTCACCCTCAGGAGCTCAGAGCCGCAGCTTCGACGGAG CACCAGCTCCTCCAGCGATACAATGAGTACTTTCAAGCCTATGGTGGCACCCAGGATGGGTGTCCAGCTG AAGCCCCCAGCCCTTAGGCCAAAGCCTGCTGTTCTTCCAAAAACAAACCCTACTGTAGGACCTGCCGCAC CTTCCCAGGGTCCCACAGACAAGTCTTGCACGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217466 representing NM_001191784 Red=Cloning site Green=Tags(s) MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKL AERFMAKTRSTKDHQQFKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMF KKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHSESISAESKLKEAEKQEEKQMGRSGDPVFHIRLEERHQ RRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRA LRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQP VQAELMLRNQQLQSRLATLKIESEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYL SKPSIAKRRANQQETEQFYFMKLREFLEGSSLITKLQAKHDLLQRTLGEGHRAEYMTTRPPNVPPKPQKH RKSRPRSQYNAKLFNGDLETFVKDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERG ENPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYERALHIHKLLLTLPRSVLIV MRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIVKTIIIHHETIFPDAKEL DGPVYEKCMAGGDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASL LLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSEASSGPVPEDKSSSKDMNSPTDRH SDSYLARQRKRGEPPPPGRRPGRTSDGHCPLHPPHALSNSSIDLGSPNLTGHPRGLLQNRGLNNDSPERR RRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQS TVKHAPDVVLDTLEQVKNSPTPATSTESLSPLHSVTLRSSEPQLRRSTSSSSDTMSTFKPMVAPRMGVQL KPPALRPKPAVLPKTNPTVGPAAPSQGPTDKSCTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191784
ORF Size	3255 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191784.1, NP_001178713.1
RefSeq Size	3258 bp
RefSeq ORF	3258 bp
Locus ID	314903
MW	124 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.