Phf20l1 (NM_001271439) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217413 representing NM_001271439 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAAAAAGCCCCCAAACCGTCCTGGAATCACTTTTGAGATTGGTGCTCGTTTGGAGGCACTCGACT ACTTACAGAAATGGTATCCATCACGAATTGAAAAAATTGATTATGAAGAAGGGAAGATGCTAGTTCATTT TGAGCGCTGGAGTCATCGTTATGATGAGTGGATTTATTGGGATAGCAACAGGTTGCGACCCCTTGAAAGA CCTTCACTAAGAAAAGAAGGGCTAAAGGACGAGGAAGATCTTTTTGATTTTAAAGCTGGAGAAGAAGTTC TGGCTCGTTGGACAGACTGTCGCTATTACCCTGCCAAGATTGAAGCAATAAACAAAGAAGGAACATTCAC AGTTCAGTTTTATGATGGCGTAATCCGTTGTTTGAAAAGAATGCACATTAAAGCCATGCCTGAGGATGCT AAGGGGCAGGTGAAATCCCAGCATCCACTAAGCTGGTGTTGTCCTATCGACCCAGCTGGATCGTGTAACC AGTCTATGGGAAGTGAGGACTGGATTGCTTTAGTCAAAGCAGCTGCTGCAGCTGCAGCGAAGAATAAAAC AGGGTCTAAACCTCGAACCAGTGCTAACAGCAATAAAGAGAAAGAGAGAGATGGGGGAAAATGGTTCAAA GTACCTTCAAAGAAGGCAGAGACTTCCACATGTATAGTCACAGCAGAGATTGAGAAGAAGGAAGAGCTAC CCACATCTAGTGAACCGTTTGGACTTCACATAGACAGCGTTCCAAAGATCGTCTTTCCACAGCCAGAGAG CACATTAACAAACAAGAGGAAAAATAATCAAGGCAACTCATTTCAGGCAAAGAGAGCCCGACTTAACAAG ATTACTGGCTTGTTGGCATCCAAGGCAGTTGGGGTGGATGGTGCTGAAAAAAAAGAAGACTGCAGTGCAA CAGCTCCAGTCCTGGAGCAGGCGATTTCACCTAAACCTCAAAGTCAGAAAAAAAATGAAGCTGTCATTAG CAGTTCTGCCAACACTCAGAAACCTGCACTGTTATCCTCAACTTTGTCTTCAGGGAAGGCTCGCAGCAAG AAATGCAAACATGAATCTGGAGAGTCTTCTGGGTGTATAAAAGCCCCTAAGTCACCACTTGCCCCAGAAT TAATACAAGCCAAGGATTTGACGCTTGTATCTCAGCTTTCTTCAGTGATAAATAAAACTAGTTCTCCACA GCCTGTGAATCCCCCTAGACCTTGCAAGCATAGTGAGAGAAGAAGAAGATCTCAGCGCTTAGCCACCTTA CCCATGCCTGATGATTCTCTAGAAAAGCTTTCTTCTTCCTCTTCAGCAACTGATGGGAAAGTATTCTCCA TCAGTTCTCAGAATCAGCAAGAGTCTTCAGTACCAGAAGTGCCTGCTATTGCATATGTGCCGCTCCAGAA GCTGGGACCCTGTCTCCCTCTTGATTTAAGTTGTGGTTCAGAAGTCACAGGAAGTCAAGCCCCAGATTCC TCTTACCCTGGTGGTGAATGTCCCAGGGAAGAAAAGGAGGAGACACCATTGTTTGCAAATCCCACCTCCA AAGTAGTGAGTGATGTAAAAGGAGCTGCAGCAGCTACAGGAATATTGAAAACAGAAAAAAAAGTGAAATT GGAAGAAAAAACCTCCACTGCATTTGGTAAAAGGAAAGAAAAGGATAAGGAGAGAAAAGAGAAGCGAGAC AAAGATCACTACAAACCAAAGCAGAAGAAAAAGAAGAAGAAGAAGAAAAAATCTAAACAGCACGACTATT CGGACTACGAAGACAGTTCCCTTGACTTTTTGGAAAGGTGTTCTTCTCCACTAACTAGATCTTCTGGGAG TTCTCTGGCTCCACGAAGCACATTTACGGAGAAAACTACAACCTATCAGTACCCAAGGGCAATTCTGTCT GTTGATCTTAGTGGTGAAAACTTGTCAGATGTGGAATTCCTGGATGACTCTTCCACAGAGAGCCTGCTTC TGAGTGGGGATGAGTACAACCAAGACTTTGATTCAACTAATTTTGAGGAGTCTCAGGATGAAGATGATGC TCTTAATGAGATTGTACGGTGTATCTGTGAACTGGATGAAGAGAATGGCTTCATGATCCAGTGTGAAGAG TGCCTATGCTGGCAGCACAGTGTGTGCATGGGGCTTCTGGAGGACAGCATTCCGGAGCAGTACATCTGCT ACATCTGTCGGGACCCTCCGGGTCAGAGATGGAATGCAAAATATCGTTATGATAAGGAATGGTTGAATAA CGGAAGAATGTATGGGTTATCTTTTGTAAAAGAAAATTATTCTCATCTCAATGCCAAAAAAATAGTTTCC ACACACCATCTTCTTGCTGATGTCTATGGTATAACTGAAGTACTGCATGGATTGCAGCTCAAGATTGGAA TACTAAAGAATAAACATCATCCTGACCTTCGTCTCTGGGCATATTCTGGGAAGAGGAAAGATCAAGATCA GGTAGTAGCTGGAGCTGAGAGAAAAGTAATACTTCAGGACACAGCTAACTCAGAAGGAAGGAAATATGTA CAAAACCACAAAGAACCACCCCTGAAAATGGAAGAAACTTACATAACAAGTGAACACAGCTATCAAAAGC CACAGAGCTTTAGTCAGGACTGCCATTCTCTCACAGACCCTGGAAGCTCAGATGATGATGATGTTAGCAG TTTTGAAGAAGATGGGGAACTCCATGTGGCAGATAATAGCCATCTTCTGTACTCAGTGAAAGAGCGTGGA GTGTCAGAGAAGAATCCAGCTTCAGAGAATAAAGTGTTTGTTTATAATGATAAAAAGGGCATGGAGGGCC CAGGAGACGCACATCTTCAGTGGCAGCTCAATCTCCTCACACATATAGAAAATGTGCAGAATGAAGTCAC CAGCAGGATGGACCTCATAGAAAAAGAAGTGGACGTTCTGGAGAGCTGGCTTGATTTCACAGGGGAATTG GAGCCACCAGATCCCCTGGCAAGACTACCCCAACTTAAGCGCCACATAAAACAACTCTTACTTGACATGG GCAAAGTACAACAAATTGCAACTCTTTGCTCTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217413 representing NM_001271439 Red=Cloning site Green=Tags(s) MSKKPPNRPGITFEIGARLEALDYLQKWYPSRIEKIDYEEGKMLVHFERWSHRYDEWIYWDSNRLRPLER PSLRKEGLKDEEDLFDFKAGEEVLARWTDCRYYPAKIEAINKEGTFTVQFYDGVIRCLKRMHIKAMPEDA KGQVKSQHPLSWCCPIDPAGSCNQSMGSEDWIALVKAAAAAAAKNKTGSKPRTSANSNKEKERDGGKWFK VPSKKAETSTCIVTAEIEKKEELPTSSEPFGLHIDSVPKIVFPQPESTLTNKRKNNQGNSFQAKRARLNK ITGLLASKAVGVDGAEKKEDCSATAPVLEQAISPKPQSQKKNEAVISSSANTQKPALLSSTLSSGKARSK KCKHESGESSGCIKAPKSPLAPELIQAKDLTLVSQLSSVINKTSSPQPVNPPRPCKHSERRRRSQRLATL PMPDDSLEKLSSSSSATDGKVFSISSQNQQESSVPEVPAIAYVPLQKLGPCLPLDLSCGSEVTGSQAPDS SYPGGECPREEKEETPLFANPTSKVVSDVKGAAAATGILKTEKKVKLEEKTSTAFGKRKEKDKERKEKRD KDHYKPKQKKKKKKKKKSKQHDYSDYEDSSLDFLERCSSPLTRSSGSSLAPRSTFTEKTTTYQYPRAILS VDLSGENLSDVEFLDDSSTESLLLSGDEYNQDFDSTNFEESQDEDDALNEIVRCICELDEENGFMIQCEE CLCWQHSVCMGLLEDSIPEQYICYICRDPPGQRWNAKYRYDKEWLNNGRMYGLSFVKENYSHLNAKKIVS THHLLADVYGITEVLHGLQLKIGILKNKHHPDLRLWAYSGKRKDQDQVVAGAERKVILQDTANSEGRKYV QNHKEPPLKMEETYITSEHSYQKPQSFSQDCHSLTDPGSSDDDDVSSFEEDGELHVADNSHLLYSVKERG VSEKNPASENKVFVYNDKKGMEGPGDAHLQWQLNLLTHIENVQNEVTSRMDLIEKEVDVLESWLDFTGEL EPPDPLARLPQLKRHIKQLLLDMGKVQQIATLCSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271439
ORF Size	3045 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271439.1, NP_001258368.1
RefSeq Size	6648 bp
RefSeq ORF	3048 bp
Locus ID	314964
UniProt ID	Q4V9H5
MW	114.1 kDa

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