Flt3 (NM_001100822) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217404 representing NM_001100822 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGTTGGCGCGGCGCAGCGACCGGCTGCCGCTGCTTGTTGTTTTGTCAGTAATGATTCTCGAGA CCGTTACAAACCAAGATCTGCCTGTGATCAAGTGTGTTTTAATCATTCATAAGAACAATGGCTCGGCGGC GGGAAAGTCATCGTCGCACGTCATGGTTCCAGAATCCCCAGAGGACCTCCGGTGTGCCCCGAAGCATCGG AGTGAAGGGGTGGTCTATGAAGCTGTCACTGTGGAGGTGGCCCAGTCTGCGTCCTTCACATTGCAAGTAC AGCTCACCACCCCGGGGGATGTTTCTTGCCTCTGGGTCTTTAAGCACAGCTCGCTGGGCTGCCAGCCACA CTTTGATTTACAGAACAGAAGAATTGTTTCCATGACCATCTTGAACGTTACAGAGACCCAGGCAGGAGAA TACCTACTCCATATTCAGAGTGAAGCCACCAACCACACGGTGCTGTTCACAGTGAATGTAAGAGATACAC AGCTGTACGTGCTAAGAAGACCTTCCTTCAGAAAGATGGAGAACCAGGACGCCCTGCTCTGCGTCTCTGA GGGTGTCCCAGAGCCCACCGTGGAGTGGGTGGTCTGCAGTTCCCACAGGGACAGCTGTAGAGAAGAAGGC TCTGCCGTTGTCAAAAAGGAGGAAAAGGTACTTCACGAGCTATTTGAAACAGACATCAGATGCTGTGCGA GAAATGCACTGGGCCGAGAATGCACCAAGCTGTTCACCATAGATCTAAACCAGGCTCCTCAGAGCACACT GCCCCAGTTATTTCTGAAAGTGGGGGAGCCCTTGTGGATAAGGTGCAAGGCCATCCATGTGAACCATGGA TTCGGGCTCACCTGGGAGCTGGGAAACAAAGTCCTGGAGGAGGGCAGCTACTTTGAGATGAGTTCCTACT CCACCAACAGATCCATGATCCGGATTCTCTTGGCTTTTGTGTCCTCTGTGGGAAGGAACAACACCGGATA TTACACCTGTTCTTCCTCAAAGCATCCCAGCCAGACAGCGTTGGTGACCATCCTAGAAAAAGGTTTCATA AATGCTACCAGCTCACAAGAAGAGTATGAAATTGACCCGTATGAAAAGTTCTGCTTCTCGGTCAGGTTTA AAGCGTACCCACAAATCCGATGCAAGTGGACCTTCTCTCAAACATCGTTTCCTTGTGAACAAAGTGGCCT GGAGGATGGGTACAGCATTTCTAAATTTTGCGATCATAAGAACAACCCAGGAGAATACATATTCCATGGA GAAAATGATGACGCCCAGTTCACCAAAATGTTCATACTGAATATAAGAAGGAAACCTCAAGTGCTAGCAA ATGCCTCAGCCAGCCAGGCATCCTGTTTCTCTGATGGCTACCCGTTACCCAACTGGACCTGGAAGAAGTG TTCGGACAAGTCACCCAACTGCACGGAGGAAATCCCAGAAGGAGTTTGGAATAAAAAGGCTAACAGAAAA GTGTTTGGACAGTGGGTGTCGAGCAGTACCCTGAATATGAGTGAGGCCGGGAGAGGGTTTCTGGTCAAAT GCTGTGCATACAATTCTATGGGCACGTCTTGCGAAACCATCCTTTTAAACTCACCAGGCCCCTTCCTTTT CATCCAAGACAACATCTCCTTCTATGCAACCATCGGGCTTTGCCCGCCTTTCATTGTCGTCCTCACTGTG CTGATCTGTCACAAATACAAAAAGCAATTTAGGTACGAAAGCCAGCTGCAGATGATCCAAGTGACCGGCC CCCTGGATAACGAGTACTTCTACATTGACTTCAGGGACTACGAATATGACCTCAAGTGGGAGTTTCCAAG GGAAAACTTAGAGTTTGGGAAGGTCCTGGGGTCCGGCGCTTTTGGGAGAGTGATGAATGCCACGGCCTAC GGGATTAGTAAAACAGGAGTCTCAATTCAGGTGGCGGTGAAGATGCTAAAAGAGAAAGCTGACAGATGTG AAAGAGAGGCGCTCATGGCTGAGCTCAAAATGATGACCCACCTGGGGCACCATGACAACATCGTGAACCT GCTGGGGGCATGCACACTGTCAGGGCCAGTGTACTTGATTTTTGAATATTGTTGCCATGGTGACCTGCTC AACTACCTAAGAAGCAAAAGAGAAAAGTTTCACAGGACGTGGACAGAGATTTTTAAGGAACATAATTTCA GTTTTTACCCCACCTTCCAGTCACATTCAAACTCCAGTATGCCGGGTTCACGAGAAGTTCAGATATACCC GCCCGTGGATCAGGTCTCAGGGTTCAATGGGAATTCAATTCATTCTGAAGGTAATATTGAGTATGAAAAC CAGAAGAGGCTGGAAGAAGAGGAGGAAGAAGATTTGAACGTGCTGACGTTTGAAGACCTCCTTTGTTTTG CGTATCAAGTGGCCAAGGGCATGGAGTTCCTGGAGTTCAAGTCGTGTGTTCACAGAGACCTGGCAGCCAG GAACGTACTGGTCACCCACGGGAAGGTGGTGAAGATCTGTGACTTTGGACTGGCCCGAGACATCCTGAGT GACTCCAGCTACGTCGTCAGGGGCAACGCACGGCTGCCAGTGAAGTGGATGGCACCTGAGAGCTTGTTTG AAGGGATCTATACAATCAAGAGTGACGTCTGGTCCTACGGCATCCTTCTCTGGGAGATATTTTCATTGGG TGTGAACCCTTACCCCGGTATTCCTGTCGATGCTAACTTCTATAAACTGATTCAGAGTGGATTTAAAATG GAGCAGCCGTTCTATGCTACGGAAGAGATATACTTTGTAATGCAATCCTGCTGGGCTTTTGACTCAAGGA AGCGGCCATCCTTCCCCAATCTGACGTCATTTTTAGGCTGTCAGCTCGCAGAGGCAGAAGCAGCGATGTA TCAGAACATGGGCGGCGATGTCCCACTGGAGCACCCAGCCATCTACCAAAACAGGCGGCCTGCCAGCAGA GAGGCAGGCTCAGAGCCGCCATCACCACAGGTTCAGGAGAAGACTCACAGAGAAAGAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217404 representing NM_001100822 Red=Cloning site Green=Tags(s) MRALARRSDRLPLLVVLSVMILETVTNQDLPVIKCVLIIHKNNGSAAGKSSSHVMVPESPEDLRCAPKHR SEGVVYEAVTVEVAQSASFTLQVQLTTPGDVSCLWVFKHSSLGCQPHFDLQNRRIVSMTILNVTETQAGE YLLHIQSEATNHTVLFTVNVRDTQLYVLRRPSFRKMENQDALLCVSEGVPEPTVEWVVCSSHRDSCREEG SAVVKKEEKVLHELFETDIRCCARNALGRECTKLFTIDLNQAPQSTLPQLFLKVGEPLWIRCKAIHVNHG FGLTWELGNKVLEEGSYFEMSSYSTNRSMIRILLAFVSSVGRNNTGYYTCSSSKHPSQTALVTILEKGFI NATSSQEEYEIDPYEKFCFSVRFKAYPQIRCKWTFSQTSFPCEQSGLEDGYSISKFCDHKNNPGEYIFHG ENDDAQFTKMFILNIRRKPQVLANASASQASCFSDGYPLPNWTWKKCSDKSPNCTEEIPEGVWNKKANRK VFGQWVSSSTLNMSEAGRGFLVKCCAYNSMGTSCETILLNSPGPFLFIQDNISFYATIGLCPPFIVVLTV LICHKYKKQFRYESQLQMIQVTGPLDNEYFYIDFRDYEYDLKWEFPRENLEFGKVLGSGAFGRVMNATAY GISKTGVSIQVAVKMLKEKADRCEREALMAELKMMTHLGHHDNIVNLLGACTLSGPVYLIFEYCCHGDLL NYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHSNSSMPGSREVQIYPPVDQVSGFNGNSIHSEGNIEYEN QKRLEEEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDILS DSSYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPVDANFYKLIQSGFKM EQPFYATEEIYFVMQSCWAFDSRKRPSFPNLTSFLGCQLAEAEAAMYQNMGGDVPLEHPAIYQNRRPASR EAGSEPPSPQVQEKTHRERS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100822
ORF Size	3000 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100822.2, NP_001094292.1
RefSeq Size	3667 bp
RefSeq ORF	3003 bp
Locus ID	140635
MW	113.6 kDa
Gene Summary	putative tyrosine kinase; quantitative trait loci candidate gene for type II Collagen induced arthritis [RGD, Feb 2006]

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