Flt3 (NM_001100822) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217404 representing NM_001100822 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGTTGGCGCGGCGCAGCGACCGGCTGCCGCTGCTTGTTGTTTTGTCAGTAATGATTCTCGAGA CCGTTACAAACCAAGATCTGCCTGTGATCAAGTGTGTTTTAATCATTCATAAGAACAATGGCTCGGCGGC GGGAAAGTCATCGTCGCACGTCATGGTTCCAGAATCCCCAGAGGACCTCCGGTGTGCCCCGAAGCATCGG AGTGAAGGGGTGGTCTATGAAGCTGTCACTGTGGAGGTGGCCCAGTCTGCGTCCTTCACATTGCAAGTAC AGCTCACCACCCCGGGGGATGTTTCTTGCCTCTGGGTCTTTAAGCACAGCTCGCTGGGCTGCCAGCCACA CTTTGATTTACAGAACAGAAGAATTGTTTCCATGACCATCTTGAACGTTACAGAGACCCAGGCAGGAGAA TACCTACTCCATATTCAGAGTGAAGCCACCAACCACACGGTGCTGTTCACAGTGAATGTAAGAGATACAC AGCTGTACGTGCTAAGAAGACCTTCCTTCAGAAAGATGGAGAACCAGGACGCCCTGCTCTGCGTCTCTGA GGGTGTCCCAGAGCCCACCGTGGAGTGGGTGGTCTGCAGTTCCCACAGGGACAGCTGTAGAGAAGAAGGC TCTGCCGTTGTCAAAAAGGAGGAAAAGGTACTTCACGAGCTATTTGAAACAGACATCAGATGCTGTGCGA GAAATGCACTGGGCCGAGAATGCACCAAGCTGTTCACCATAGATCTAAACCAGGCTCCTCAGAGCACACT GCCCCAGTTATTTCTGAAAGTGGGGGAGCCCTTGTGGATAAGGTGCAAGGCCATCCATGTGAACCATGGA TTCGGGCTCACCTGGGAGCTGGGAAACAAAGTCCTGGAGGAGGGCAGCTACTTTGAGATGAGTTCCTACT CCACCAACAGATCCATGATCCGGATTCTCTTGGCTTTTGTGTCCTCTGTGGGAAGGAACAACACCGGATA TTACACCTGTTCTTCCTCAAAGCATCCCAGCCAGACAGCGTTGGTGACCATCCTAGAAAAAGGTTTCATA AATGCTACCAGCTCACAAGAAGAGTATGAAATTGACCCGTATGAAAAGTTCTGCTTCTCGGTCAGGTTTA AAGCGTACCCACAAATCCGATGCAAGTGGACCTTCTCTCAAACATCGTTTCCTTGTGAACAAAGTGGCCT GGAGGATGGGTACAGCATTTCTAAATTTTGCGATCATAAGAACAACCCAGGAGAATACATATTCCATGGA GAAAATGATGACGCCCAGTTCACCAAAATGTTCATACTGAATATAAGAAGGAAACCTCAAGTGCTAGCAA ATGCCTCAGCCAGCCAGGCATCCTGTTTCTCTGATGGCTACCCGTTACCCAACTGGACCTGGAAGAAGTG TTCGGACAAGTCACCCAACTGCACGGAGGAAATCCCAGAAGGAGTTTGGAATAAAAAGGCTAACAGAAAA GTGTTTGGACAGTGGGTGTCGAGCAGTACCCTGAATATGAGTGAGGCCGGGAGAGGGTTTCTGGTCAAAT GCTGTGCATACAATTCTATGGGCACGTCTTGCGAAACCATCCTTTTAAACTCACCAGGCCCCTTCCTTTT CATCCAAGACAACATCTCCTTCTATGCAACCATCGGGCTTTGCCCGCCTTTCATTGTCGTCCTCACTGTG CTGATCTGTCACAAATACAAAAAGCAATTTAGGTACGAAAGCCAGCTGCAGATGATCCAAGTGACCGGCC CCCTGGATAACGAGTACTTCTACATTGACTTCAGGGACTACGAATATGACCTCAAGTGGGAGTTTCCAAG GGAAAACTTAGAGTTTGGGAAGGTCCTGGGGTCCGGCGCTTTTGGGAGAGTGATGAATGCCACGGCCTAC GGGATTAGTAAAACAGGAGTCTCAATTCAGGTGGCGGTGAAGATGCTAAAAGAGAAAGCTGACAGATGTG AAAGAGAGGCGCTCATGGCTGAGCTCAAAATGATGACCCACCTGGGGCACCATGACAACATCGTGAACCT GCTGGGGGCATGCACACTGTCAGGGCCAGTGTACTTGATTTTTGAATATTGTTGCCATGGTGACCTGCTC AACTACCTAAGAAGCAAAAGAGAAAAGTTTCACAGGACGTGGACAGAGATTTTTAAGGAACATAATTTCA GTTTTTACCCCACCTTCCAGTCACATTCAAACTCCAGTATGCCGGGTTCACGAGAAGTTCAGATATACCC GCCCGTGGATCAGGTCTCAGGGTTCAATGGGAATTCAATTCATTCTGAAGGTAATATTGAGTATGAAAAC CAGAAGAGGCTGGAAGAAGAGGAGGAAGAAGATTTGAACGTGCTGACGTTTGAAGACCTCCTTTGTTTTG CGTATCAAGTGGCCAAGGGCATGGAGTTCCTGGAGTTCAAGTCGTGTGTTCACAGAGACCTGGCAGCCAG GAACGTACTGGTCACCCACGGGAAGGTGGTGAAGATCTGTGACTTTGGACTGGCCCGAGACATCCTGAGT GACTCCAGCTACGTCGTCAGGGGCAACGCACGGCTGCCAGTGAAGTGGATGGCACCTGAGAGCTTGTTTG AAGGGATCTATACAATCAAGAGTGACGTCTGGTCCTACGGCATCCTTCTCTGGGAGATATTTTCATTGGG TGTGAACCCTTACCCCGGTATTCCTGTCGATGCTAACTTCTATAAACTGATTCAGAGTGGATTTAAAATG GAGCAGCCGTTCTATGCTACGGAAGAGATATACTTTGTAATGCAATCCTGCTGGGCTTTTGACTCAAGGA AGCGGCCATCCTTCCCCAATCTGACGTCATTTTTAGGCTGTCAGCTCGCAGAGGCAGAAGCAGCGATGTA TCAGAACATGGGCGGCGATGTCCCACTGGAGCACCCAGCCATCTACCAAAACAGGCGGCCTGCCAGCAGA GAGGCAGGCTCAGAGCCGCCATCACCACAGGTTCAGGAGAAGACTCACAGAGAAAGAAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100822
Insert Size	3003 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100822.2, NP_001094292.1
RefSeq Size	3667 bp
RefSeq ORF	3003 bp
Locus ID	140635
Gene Summary	putative tyrosine kinase; quantitative trait loci candidate gene for type II Collagen induced arthritis [RGD, Feb 2006]

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