Dsg3 (NM_001191079) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217380 representing NM_001191079 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGCCTATTCCCCAGAGCCCTGAGGCTTCTGTCCCTCCTGATGGTGGTGCTCATGGTGTACGGGG AACTACACATAGAGTCGGGAGGACAGCACAGAGAAGACAGGATGGCATTGCAGCTGACCAAAAGGAGGTA CAAACGTGAATGGGTGAAATTTGCAAAGCCCTGCAGAGAAAGAGAAGACAACTCAAAACGAAACCCAATT GCCAAGATCACTTCAGATTTCCAAAAAAATCAGAAAATTACTTACCGCATTTCCGGAGTGGGAATTGATC AGCCCCCTTTCGGGATCTTTGTTGTTGACCCAAACAATGGCGACATCAACATAACAGCCATAGTTGATCG TGAGGAAACCCCAAGCTTCCTGATCACATGCCGAGCCCTTAATGCCCTGGGACAAGATGTAGAGAGACCA CTTATACTGACAGTGAAAATTTTAGATGTCAATGATAATGCTCCAATATTTTCACAAACCATATTCAAGG GTGAAATTGAAGAAAACAGTGCTTCAAATTCCCTGGTGATGATCCTAAATGCCACAGACGCGGACGAGCC AAACCATATGAACTCTAAAATCGCCTTCAAAATTGTCTCCCAGGAGCCCGCCGGCATGTCCATGTTCCTC ATCAGCAGAAACACCGGAGAAGTCCGCACTTTGACCAGTTCTCTCGATCGAGAGCAAGTTGGTAGCTACC ATTTGATTGTGAGCGGTGCAGACAACGATGGAACAGGACTGTCGACTCAGTGTGAATGTAGTATCAAAAT TAAAGATGTCAACGACAACTTCCCAGTGCTTACGGACTCGCAGTACTCAGCACGTATTGAGGAAAATACT TTGAATTCAGAGTTACTCCGATTTCAAGTGACAGACTGGGATGAAGAATACACAGATAATTGGTTGGCTG TGTATTTCTTTACCTCTGGGAATGAAGGGAATTGGTTTGAAATCGAAACAGATCCTAGAACCAATGAAGG CATCCTGAAGTTGGTTAAGGTTCTGGATTATGAACAAATGCAAAGTATGCAATTTAGTATTGCCGTGAGG AACAAAGCTGAGTTCCACCAGTCAGTGATCTCTCAGTACCAAGTGCAGTCAACTCCAGTCACAATTCAAG TCATCAATGTCCAAGAGGGGATTTCATTCCGTCCTCCTTCCAGGACATTTACTGTGCAAAGGGGTGTAAG CATTAACAAATTAGCTGGTTACATCCTGGGGACATACCAAGCGACGGATGAGGACACTGGTAAAGCTGCC TCCTCTGTCAGATATGTCCTGGGTCGTAATGATGGTGGTTTTCTTGTTATTGATTCAAAAACTGCTGAGA TCAAATTTGTAAAAAACATCAACCGAGATTCCACTTTCATAGTTAACAAGACAATCTCTGCTGAGGTTCT GGCCATAGACGAAAACACTGGTAAGACCTCCACAGGCACAGTATATGTGGAAGTGCCCAGTTTTAATGAA AACTGTCCATCAGTTGTCCTGGAGAAGAGAGAGATCTGTACATCCTCACCATCGGTGACCCTCTCAGTGA GAACCCTGGACAGGGCTAAATACACTGGCCCCTACACAGTGTCTCTGGAGGAGCAGCCACTGAAGCTGCC GGTTGTGTGGACCATCACGACGCTGAATGCTACCTCAGCACTTCTACAAGCCCAGCAGCATATCTCTCCA GGAGTGTACAATGTCCCAGTCATCGTGAAGGACAATCAGGATGGGCAGTGTGACACACTAGAGAGCTTGA CCCTGACAGTGTGTCAGTGTGATGACCGGAGCATGTGCAGAGCCCCTGGCCCGAGCAGAGAGCCTTTTAT CCGTGAGGAGTCAACCTGGAGGCTGGGGCCTGCTGCTATTGGCTTGATCCTGCTAGGACTCCTGATGTTG CTACTGGCCCCACTTCTGCTTCTGACCTGTGACTGTGGGTCAGGTCCCATCGGAGGAGCAGGGACAGGTG GCTTTGTCCCAGTGCCCGATGGCTCGGAAGGAACGATTCATCAGTGGGGAATCGAAGGAGCTCAGCCCGA AGACAAGGAAATCACAAATATTTGTGTTCCTCCAGTCACAAGCAATGGAGCAGATTTTATGGAAAGTTCT GAAGTTTGCACAAATACCTACGCTGGAGGGACCCTAGTAGAAGGTGCTTCCGGAATGGAGATGACTACCA AGCTAGGAGGAGCTACGGGATCAGGAGCTGCAGTGGCGCTTGGCTTGGGTCATTCAGGAACGATGAGAAC GAGGCATTCCACTGGAGGGACCTTGAAGGATTATGCTGCACCAGTGAACATGACTTTCCTAGGATCCTAC TTCTCTCAGAAATCATCCGCCTATGCTGAGGAAGAGGATGATCGTGAAGTGAATGACTGTTTGCTGATCT ACGATGACGAAGGCGAGGATGCTGCCCCCCGTTCCCCAGCCCTCAGCTCCTGCAGCTTTATGGCCGATGA CCTGGACGACAGTTTCTTGGACTCCCTTGGCCCCAAATTTAAAAAGCTTGCAGAGATATGTCTTGGCGTT GATGACGAAGCCAAGCAAGTAAAGCCAGGCCCTAAAGACAGTAGTTCTGGGGCTGACGCCTGTGCTCGCC CTACAGAAGCCCTTCAGGCAGGTTCCAAAGTGTGCCACGTGTTGCCTGGCAACCAAGATACTTCTGTTTT GTCCTCTTCTGGGTCTGTCCACCCTGCTATTGCCATCCCTGATCCTCTACAGCTTGGTAACTACTTGCTC ACAGAAACTTACACAACATCAGGTTCCTTTGTGCAACCCACCACTGCCACCTTTGACCCACATGTCACTC AAAATGTCACTGTAACAGAAAGAGTCATTTGCCCCCTTCCTAGTGCCTCTGGCAGCATAGTGGCTCCCAC AGAGCTTCGAGGCTCCTACAGTATGCTCTATACAAAAGAAGCCTGTTCCCATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217380 representing NM_001191079 Red=Cloning site Green=Tags(s) MACLFPRALRLLSLLMVVLMVYGELHIESGGQHREDRMALQLTKRRYKREWVKFAKPCREREDNSKRNPI AKITSDFQKNQKITYRISGVGIDQPPFGIFVVDPNNGDINITAIVDREETPSFLITCRALNALGQDVERP LILTVKILDVNDNAPIFSQTIFKGEIEENSASNSLVMILNATDADEPNHMNSKIAFKIVSQEPAGMSMFL ISRNTGEVRTLTSSLDREQVGSYHLIVSGADNDGTGLSTQCECSIKIKDVNDNFPVLTDSQYSARIEENT LNSELLRFQVTDWDEEYTDNWLAVYFFTSGNEGNWFEIETDPRTNEGILKLVKVLDYEQMQSMQFSIAVR NKAEFHQSVISQYQVQSTPVTIQVINVQEGISFRPPSRTFTVQRGVSINKLAGYILGTYQATDEDTGKAA SSVRYVLGRNDGGFLVIDSKTAEIKFVKNINRDSTFIVNKTISAEVLAIDENTGKTSTGTVYVEVPSFNE NCPSVVLEKREICTSSPSVTLSVRTLDRAKYTGPYTVSLEEQPLKLPVVWTITTLNATSALLQAQQHISP GVYNVPVIVKDNQDGQCDTLESLTLTVCQCDDRSMCRAPGPSREPFIREESTWRLGPAAIGLILLGLLML LLAPLLLLTCDCGSGPIGGAGTGGFVPVPDGSEGTIHQWGIEGAQPEDKEITNICVPPVTSNGADFMESS EVCTNTYAGGTLVEGASGMEMTTKLGGATGSGAAVALGLGHSGTMRTRHSTGGTLKDYAAPVNMTFLGSY FSQKSSAYAEEEDDREVNDCLLIYDDEGEDAAPRSPALSSCSFMADDLDDSFLDSLGPKFKKLAEICLGV DDEAKQVKPGPKDSSSGADACARPTEALQAGSKVCHVLPGNQDTSVLSSSGSVHPAIAIPDPLQLGNYLL TETYTTSGSFVQPTTATFDPHVTQNVTVTERVICPLPSASGSIVAPTELRGSYSMLYTKEACSHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191079
ORF Size	2925 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191079.1, NP_001178008.1
RefSeq Size	2928 bp
RefSeq ORF	2928 bp
Locus ID	291752
MW	106 kDa

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