Pde2a (NM_001270604) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CGS-PDE; Pde2; Pde2a2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217319 representing NM_001270604 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCGACAGCCTGCAGCCAGCCAGGACCCCCTTGCACAGAAGCCAGAGCCCCCAGGGAGTAGAGACG ACCGACTGGAGGATGCTTTGCTGAGCCTGGGCGCCGTTATCGACATCGCGGGCCTTCGACAGGCTGCCAA GGATGCCCTCTCGGCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGGTAGATGGGGAGTCCAGG CTGGTGTGTGAGGACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGAGGCTGTGATTTCTCGGA AGCGGCTGAGCTGCGATGGGCTGGGACCTTCAGACCTACTAGGAAAGCCCTTGGCCAGGCTGGTGGCTCC ACTGGCCCCTGACACGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTGGAACTGTGGCAGCCGTC ATCTTGGTGCACTGTGGCCAGCTGAGTGACAGTGAGGAGCAGAGCCTGCAGGTGGTGGAGAAGCATGCTC TGGTGGCCCTGCAGAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTTCAGAACACCTCAGCGGA TCCCTCTGAAGATCAAAAGGATGAGAAGGGATACACCGCCCATGACCGAAAGATCCTGCAACTGTGTGGA GAACTCTATGACTTGGATGCCACTTCTCTGCAGCTCAAAGTCCTTCGATATCTTCAGCAGGAGACACAGG CCACTCACTGCTGCCTCCTGCTGGTGTCAGAGGACAACCTGCAGCTTTCCTGCAAGGTCATTGGAGAGAA AGTGCTGGGAGAAGAGGTCAGCTTTCCATTGACCATGGGACGCCTGGGCCAGGTGGTGGAGGACAAACAG TGTATCCAGTTGAAGGACCTAACCTCTGACGATGTGCAACAGCTACAAAACATGTTGGGCTGTGAGCTTC GGGCTATGCTATGTGTCCCTGTCATCAGTCGAGCCACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAA CAAGCTTGGAGGAGACTTCTTCACAGATGAGGATGAACGTGCGATCCAACACTGCTTCCACTACACAGGC ACGGTGCTCACCAGTACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCC AAGTGGCAAAGAACCTCTTCACCCACCTGGATGACGTCTCTGTCCTGCTACAGGAGATCATCACAGAGGC CAGAAACCTCAGCAACGCAGAGATCTGCTCCGTGTTCCTGCTGGATCAGAATGAGCTGGTTGCCAAGGTG TTCGATGGCGGAGTAGTGGACGATGAGAGTTATGAGATCCGCATCCCTGCGGACCAAGGCATCGCGGGCC ACGTGGCGACCACGGGCCAGATCCTGAACATCCCAGATGCATACGCCCATCCGCTTTTCTATCGCGGCGT AGACGACAGCACTGGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGAACGAGAACCAAGAGGTC ATCGGTGTGGCTGAGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAATTTGATGAGGACCTGGCCA CAGCCTTCTCCATCTACTGTGGCATTAGCATCGCTCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCA GTACCGCAGCCACCTGGCCAACGAGATGATGATGTACCATATGAAGGTCTCTGACGATGAATACACCAAA CTTCTCCATGATGGCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAACTTCACCTATACTCCTC GGTCTCTGCCTGAGGACGATACTTCTATGGCCATCCTGAGCATGCTGCAAGACATGAACTTCATCAATAA CTACAAAATTGACTGCCCAACTCTGGCCCGGTTCTGCCTGATGGTGAAGAAAGGCTACCGGGATCCGCCC TACCACAACTGGATGCACGCCTTCTCTGTCTCTCACTTTTGCTACTTGCTCTACAAGAATCTGGAGCTCT CCAACTACCTCGAGGACATCGAGATCTTTGCGTTGTTTATTTCCTGCATGTGTCACGACCTGGACCACAG AGGCACAAACAACTCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCTGCACTCTACAGCTCAGAGGGCTCT GTCATGGAGAGGCACCACTTTGCTCAGGCCATTGCTATCCTCAACACCCATGGCTGTAATATCTTTGACC ACTTCTCTCGGAAGGACTATCAGCGAATGCTGGACCTGATGAGGGACATCATCTTGGCCACGGACCTGGC ACACCACCTCCGCATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATGACCGAAACAACAAGCAA CACCACAGGCTTCTTCTATGCCTCCTTATGACCTCCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGA CCACCAGAAAGATTGCGGAGCTGATCTACAAAGAGTTCTTCTCCCAAGGAGACTTGGAGAAGGCCATGGG CAACCGACCAATGGAGATGATGGATCGGGAGAAGGCCTACATCCCTGAACTTCAGATCAGCTTTATGGAG CACATCGCCATGCCCATCTACAAGCTGTTACAAGACCTGTTCCCCAAGGCAGCAGAGCTGTATGAACGCG TGGCCTCCAATCGTGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAA CTCGCTGGATTTCCTGGATGAGGAATATGAGGTCCCTGATTTGGACGTCACCAGGGCTCCTGTCAATGGC TGCTGCAGCCTCGAGGGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217319 representing NM_001270604 Red=Cloning site Green=Tags(s) MRRQPAASQDPLAQKPEPPGSRDDRLEDALLSLGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESR LVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAV ILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKILQLCG ELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQ CIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDEDERAIQHCFHYTG TVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKV FDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEV IGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTK LLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPP YHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGS VMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQ HHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFME HIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDVTRAPVNG CCSLEG SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270604
ORF Size	2748 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270604.2
RefSeq Size	4116 bp
RefSeq ORF	2751 bp
Locus ID	81743
MW	103.3 kDa
Gene Summary	activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006]

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