Pde2a (NM_001270604) Rat Tagged ORF Clone
CAT#: RR217319
- TrueORF®
Pde2a (myc-DDK-tagged) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3
"NM_001270604" in other vectors (1)
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CNY 8,840.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CGS-PDE; Pde2; Pde2a2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR217319 representing NM_001270604
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCGACAGCCTGCAGCCAGCCAGGACCCCCTTGCACAGAAGCCAGAGCCCCCAGGGAGTAGAGACG ACCGACTGGAGGATGCTTTGCTGAGCCTGGGCGCCGTTATCGACATCGCGGGCCTTCGACAGGCTGCCAA GGATGCCCTCTCGGCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGGTAGATGGGGAGTCCAGG CTGGTGTGTGAGGACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGAGGCTGTGATTTCTCGGA AGCGGCTGAGCTGCGATGGGCTGGGACCTTCAGACCTACTAGGAAAGCCCTTGGCCAGGCTGGTGGCTCC ACTGGCCCCTGACACGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTGGAACTGTGGCAGCCGTC ATCTTGGTGCACTGTGGCCAGCTGAGTGACAGTGAGGAGCAGAGCCTGCAGGTGGTGGAGAAGCATGCTC TGGTGGCCCTGCAGAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTTCAGAACACCTCAGCGGA TCCCTCTGAAGATCAAAAGGATGAGAAGGGATACACCGCCCATGACCGAAAGATCCTGCAACTGTGTGGA GAACTCTATGACTTGGATGCCACTTCTCTGCAGCTCAAAGTCCTTCGATATCTTCAGCAGGAGACACAGG CCACTCACTGCTGCCTCCTGCTGGTGTCAGAGGACAACCTGCAGCTTTCCTGCAAGGTCATTGGAGAGAA AGTGCTGGGAGAAGAGGTCAGCTTTCCATTGACCATGGGACGCCTGGGCCAGGTGGTGGAGGACAAACAG TGTATCCAGTTGAAGGACCTAACCTCTGACGATGTGCAACAGCTACAAAACATGTTGGGCTGTGAGCTTC GGGCTATGCTATGTGTCCCTGTCATCAGTCGAGCCACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAA CAAGCTTGGAGGAGACTTCTTCACAGATGAGGATGAACGTGCGATCCAACACTGCTTCCACTACACAGGC ACGGTGCTCACCAGTACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCC AAGTGGCAAAGAACCTCTTCACCCACCTGGATGACGTCTCTGTCCTGCTACAGGAGATCATCACAGAGGC CAGAAACCTCAGCAACGCAGAGATCTGCTCCGTGTTCCTGCTGGATCAGAATGAGCTGGTTGCCAAGGTG TTCGATGGCGGAGTAGTGGACGATGAGAGTTATGAGATCCGCATCCCTGCGGACCAAGGCATCGCGGGCC ACGTGGCGACCACGGGCCAGATCCTGAACATCCCAGATGCATACGCCCATCCGCTTTTCTATCGCGGCGT AGACGACAGCACTGGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGAACGAGAACCAAGAGGTC ATCGGTGTGGCTGAGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAATTTGATGAGGACCTGGCCA CAGCCTTCTCCATCTACTGTGGCATTAGCATCGCTCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCA GTACCGCAGCCACCTGGCCAACGAGATGATGATGTACCATATGAAGGTCTCTGACGATGAATACACCAAA CTTCTCCATGATGGCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAACTTCACCTATACTCCTC GGTCTCTGCCTGAGGACGATACTTCTATGGCCATCCTGAGCATGCTGCAAGACATGAACTTCATCAATAA CTACAAAATTGACTGCCCAACTCTGGCCCGGTTCTGCCTGATGGTGAAGAAAGGCTACCGGGATCCGCCC TACCACAACTGGATGCACGCCTTCTCTGTCTCTCACTTTTGCTACTTGCTCTACAAGAATCTGGAGCTCT CCAACTACCTCGAGGACATCGAGATCTTTGCGTTGTTTATTTCCTGCATGTGTCACGACCTGGACCACAG AGGCACAAACAACTCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCTGCACTCTACAGCTCAGAGGGCTCT GTCATGGAGAGGCACCACTTTGCTCAGGCCATTGCTATCCTCAACACCCATGGCTGTAATATCTTTGACC ACTTCTCTCGGAAGGACTATCAGCGAATGCTGGACCTGATGAGGGACATCATCTTGGCCACGGACCTGGC ACACCACCTCCGCATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATGACCGAAACAACAAGCAA CACCACAGGCTTCTTCTATGCCTCCTTATGACCTCCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGA CCACCAGAAAGATTGCGGAGCTGATCTACAAAGAGTTCTTCTCCCAAGGAGACTTGGAGAAGGCCATGGG CAACCGACCAATGGAGATGATGGATCGGGAGAAGGCCTACATCCCTGAACTTCAGATCAGCTTTATGGAG CACATCGCCATGCCCATCTACAAGCTGTTACAAGACCTGTTCCCCAAGGCAGCAGAGCTGTATGAACGCG TGGCCTCCAATCGTGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAA CTCGCTGGATTTCCTGGATGAGGAATATGAGGTCCCTGATTTGGACGTCACCAGGGCTCCTGTCAATGGC TGCTGCAGCCTCGAGGGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR217319 representing NM_001270604
Red=Cloning site Green=Tags(s) MRRQPAASQDPLAQKPEPPGSRDDRLEDALLSLGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESR LVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAV ILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKILQLCG ELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVSFPLTMGRLGQVVEDKQ CIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGGDFFTDEDERAIQHCFHYTG TVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKV FDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEV IGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTK LLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPP YHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGS VMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQ HHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFME HIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLDVTRAPVNG CCSLEG SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001270604 |
ORF Size | 2748 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001270604.2 |
RefSeq Size | 4116 bp |
RefSeq ORF | 2751 bp |
Locus ID | 81743 |
MW | 103.3 kDa |
Gene Summary | activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN217319 | Pde2a (untagged) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3 |
CNY 8,840.00 |