Arhgap17 (NM_001270692) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Rich1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217269 representing NM_001270692 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGCAATTCAACCGCATGAAGCAGCTGGCCAACCAGACTGTCGGCAGAGCTGAGAAGACAGAAG TCCTCAGTGAAGATCTGCTACAGATTGAACGTCGACTGGACACTGTGCGGTCAATGTGCCACCATTCACA TAAGCGTTTGATAGCCTGCTTCCAAGGTCAGCATGGCACCGATGCTGAGAGGAGACATAAAAAGCTTCCT CTGACAGCTCTCGCCCAGAACATGCAGGAAGCTTCAGCCCAGCTGGAAGAGTCTCTCTTGGGGAAGATGC TGGAAACCTGTGGGGATGCTGAGAATCAGCTGGCTTTTGAACTCTCTCAGCATGAGGTCTTTGTGGAGAA GGAGATCATGGACCCTCTCTACGGCATAGCGGAGGTGGAGATTCCCAACATCCAAAAGCAAAGGAAACAG CTTGCTAGATTGGTGCTGGACTGGGATTCAGTCAGAGCAAGATGGAACCAAGCACACAAGTCTTCAGGAA CCAACTTTCAGGGACTTCCATCTAAAATAGATACCCTAAAGGAAGAGATGGATGAAGCTGGAAATAAAGT CGAGCAGTGCAAGGATCAACTTGCAGCAGACATGTACAACTTCATGGCCAAAGAAGGGGAATACGGCAAG TTCTTTGTGACGTTATTAGAAGCCCAAGCAGATTACCATAGAAAAGCATTAGCAGTCTTAGAAAAGGCCC TTCCCGAAATGCGCGCCCATCAAGATAAATGGGCAGAGAAGCCAGCCTTCGGTACACCTCTGGAGGAACA CCTGAAGAGGAGTGGACGTGAGATCGCTCTCCCTATTGAGGCCTGCGTCATGTTGTTACTGGAGACTGGC ATGAAGGAGGAGGGCCTTTTTCGGATCGGAGCTGGAGCCTCCAAGTTGAAGAAGCTGAAAGCTGCTCTCG ACTGCTCCACGTCTCACCTGGATGAGTTCTATTCAGACCCCCATGCTGTCGCAGGTGCTTTAAAGTCCTA TTTGCGGGAACTGCCTGAGCCCTTAATGACTTTCAGTCTGTATGAAGAGTGGACACAAGTTGCAAGTGTG CAGGATCAAGACAAAAAACTTCAATATTTATGGACAACATGTCAGAAGTTGCCGCCACAAAATTTTGTTA ACTTTAGGTATTTAATCAAGTTCCTTGCAAAGCTCGCCCAGACCAGTGATGTTAATAAAATGACTCCTAG CAACATAGCCATCGTGTTGGGCCCTAACCTCCTGTGGGCCAAACAAGAAGGAACCCTGGCTGAAATAGCG GCTGCCACATCTGTCCACGTGGTCGCAGTGATTGAACCTATCATCCAGCATGCCGATTGGTTCTTCCCCG GAGAGGTAGAATTCAATGTATCAGAAGCATTCGTGCCTCTTGCTACCCCAAATTCTAATCACTCATCCCA TACTGGAAATGACTCTGACTCGGGGACTCTGGAGAGGAAGCGACCCGCCAGCATGGCAGTGATGGAAGGG GACTTGGTGAAGAAGGAGAGCTTTGGTGTGAAGCTTATGGACTTCCAGGCCCACCGGCGGGGTGGCACTC TAAATAGAAAGCACATATCTCCTGCTTTCCAGCCGCCACTCCCGCCCACGGATGGCAACGCCTTGGCTCC AGCAGGCCCAGAGCTCCCTTCTCAGAGCTCTAGGGCTGACAGCAACTCAGTGGGTGGGCCTGTCCCCTCT TCTTCGGGCATATTGGAGCAGGGGCTGAGCCCAGGTGACAGCAGTCCTCCAAAACCCAAAGACTCTGTGT CTGCAGCTGCCCCTGTGGCAGGAAGAAACAGCAACCAGATAACCACGGTCCCAAACCAGGCCCAGACAGG TGGCAACTCCCATCAGCTCTCAGTAGGCACAGCTCACAGTGCAGCTGGCCCCAGCCCGCACACTCTGCGT CGAGCTGTGAAGAAACCTGCTCCGGCACCCCCCAAACCAGGAAACCCACCTCCTGGCCACCCTGGAGGAC AGAGCTCTCCAGGCACAGGCACATCCCCAAAGCCAAGCACCCGAAGCCCATCACCGCCTCAGCAGCAGCA GCAGCAGCAACAACAACAACAGCAGCAGCAACAACAACAGCAGCAACAACAACAACAACAACAGCAGCAG CAGCAGACCCCGGGCATGCGCCGCTGTTCCAGCAGCCTGCCTCCTATCCAGGCACCCAACCACCCACCAC CGCAGCCCCCCACACAGCCTCGGCTGGGTGAACAGGGACCAGAGCCAGGCCCCACGCCACCTCAGACCCC CACACCACCCAGCACCCCACCCCCGGCCAAGCAGAATTCATCCCAAAGCGAGACCACACAGCTGCATGGA ACCCTCCCAAGGCCACGGCCAGTGCCTAAACCCCGCAACCGGCCTAGTGTGCCACCACCCCCGAATCCAC CTGGCACCCACATGGGGGATGGTGGCCTCACACCTTCAGTGCCCACAGCCTCCAGAATCGTCACTGACAC CAATTCTAGGGTTTCTGAATCACTTCGAAACATCTTTCCTGAAATACATTCAGACTTGGCAAGCAAAGAA GTGCCTGGCCATATCCTGCTGGACATAGACAATGACACAGAGAGCACCGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217269 representing NM_001270692 Red=Cloning site Green=Tags(s) MKKQFNRMKQLANQTVGRAEKTEVLSEDLLQIERRLDTVRSMCHHSHKRLIACFQGQHGTDAERRHKKLP LTALAQNMQEASAQLEESLLGKMLETCGDAENQLAFELSQHEVFVEKEIMDPLYGIAEVEIPNIQKQRKQ LARLVLDWDSVRARWNQAHKSSGTNFQGLPSKIDTLKEEMDEAGNKVEQCKDQLAADMYNFMAKEGEYGK FFVTLLEAQADYHRKALAVLEKALPEMRAHQDKWAEKPAFGTPLEEHLKRSGREIALPIEACVMLLLETG MKEEGLFRIGAGASKLKKLKAALDCSTSHLDEFYSDPHAVAGALKSYLRELPEPLMTFSLYEEWTQVASV QDQDKKLQYLWTTCQKLPPQNFVNFRYLIKFLAKLAQTSDVNKMTPSNIAIVLGPNLLWAKQEGTLAEIA AATSVHVVAVIEPIIQHADWFFPGEVEFNVSEAFVPLATPNSNHSSHTGNDSDSGTLERKRPASMAVMEG DLVKKESFGVKLMDFQAHRRGGTLNRKHISPAFQPPLPPTDGNALAPAGPELPSQSSRADSNSVGGPVPS SSGILEQGLSPGDSSPPKPKDSVSAAAPVAGRNSNQITTVPNQAQTGGNSHQLSVGTAHSAAGPSPHTLR RAVKKPAPAPPKPGNPPPGHPGGQSSPGTGTSPKPSTRSPSPPQQQQQQQQQQQQQQQQQQQQQQQQQQQ QQTPGMRRCSSSLPPIQAPNHPPPQPPTQPRLGEQGPEPGPTPPQTPTPPSTPPPAKQNSSQSETTQLHG TLPRPRPVPKPRNRPSVPPPPNPPGTHMGDGGLTPSVPTASRIVTDTNSRVSESLRNIFPEIHSDLASKE VPGHILLDIDNDTESTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270692
ORF Size	2574 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270692.1, NP_001257621.1
RefSeq Size	3378 bp
RefSeq ORF	2577 bp
Locus ID	63994
UniProt ID	Q99N37
MW	93.8 kDa
Gene Summary	activates RhoA, Rac1, and Cdc42 GTPases, plays a role in regulation of calcium ion dependent exocytosis, may regulate actin filament reorganization [RGD, Feb 2006]

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