Ago1 (NM_001191765) Rat Tagged ORF Clone
CAT#: RR217268
- TrueORF®
Ago1 (myc-DDK-tagged) - Rat argonaute RISC catalytic component 1 (Ago1)
"NM_001191765" in other vectors (1)
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CNY 8,270.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Eif2c1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR217268 representing NM_001191765
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCGGGACCCTCGGGAGCAGCTGCAGGTGCCTACCTGCCTCCCCTGCAGCAGGTGTTCCAGGCCC CTCGCCGGCCTGGCATCGGCACTGTGGGCAAACCGATCAAGCTTCTGGCCAATTACTTTGAGGTGGACAT TCCTAAGATTGACGTCTACCATTACGAGGTGGACATCAAGCCGGATAAGTGTCCTCGCAGAGTCAACCGG GAGGTGGTGGAGTACATGGTCCAGCATTTCAAACCGCAGATCTTTGGGGATCGCAAGCCTGTGTACGATG GAAAGAAGAACATTTACACAGTTACAGCACTGCCTATTGGCAATGAGAGGGTTGACTTTGAGGTGACCAT CCCTGGGGAAGGGAAGGATAGAATTTTTAAGGTCTCCATCAAGTGGCTAGCCATCGTGAGCTGGCGCATG CTGCATGAAGCCTTGGTCAGTGGCCAGATCCCTGTGCCCTTGGAGTCCGTGCAAGCCCTGGATGTGGCCA TGAGGCATCTGGCATCTATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTA CCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAG ATGATGCTCAACATTGATGTCTCAGCCACTGCCTTCTACAAAGCACAGCCAGTGATTGAGTTCATGTGTG AGGTCCTGGACATCAGGAACATAGATGAACAGCCCAAGCCCCTCACGGATTCCCAGCGCGTTCGGTTTAC CAAGGAGATAAAGGGCCTGAAGGTGGAAGTGACCCACTGTGGACAGATGAAGAGGAAATACCGCGTGTGT AATGTTACCCGTCGCCCTGCTAGCCATCAGACGTTTCCCTTGCAGCTGGAGAGCGGACAGACCGTGGAGT GCACCGTGGCACAATATTTCAAGCAGAAATACAACCTTCAGCTCAAGTATCCTCACCTGCCCTGCCTGCA AGTTGGGCAAGAGCAGAAGCATACCTATTTGCCCCTCGAGGTCTGTAACATTGTGGCTGGGCAGCGGTGC ATTAAGAAGCTGACTGACAACCAGACTTCAACCATGATAAAGGCTACAGCTAGGTCGGCCCCAGACAGAC AGGAGGAGATCAGTCGCCTGATGAAGAACGCCAGCTACAACTTGGATCCCTACATCCAGGAATTTGGAAT CAAAGTGAAGGATGACATGACGGAGGTGACGGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCGGC CGGAACCGGGCCATTGCTACACCCAACCAAGGTGTCTGGGACATGCGTGGGAAACAGTTCTACAATGGGA TTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAAAAACAATGTCGAGAAGAGGTGCTCAAGAA CTTCACGGACCAGCTTCGGAAGATCTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGTTTCTGC AAATACGCACAGGGGGCGGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAATACCTACTCAGGACTGC AGCTCATTATTGTCATCCTGCCCGGGAAGACGCCGGTGTATGCTGAAGTGAAGCGTGTTGGAGATACGCT CTTGGGAATGGCGACACAGTGTGTGCAGGTGAAGAATGTGGTCAAGACCTCACCTCAGACTCTGTCCAAC CTCTGCCTCAAGATCAATGTCAAACTCGGTGGCATCAACAACATCCTTGTCCCACACCAGCGCTCTGCTG TTTTCCAACAGCCAGTGATTTTTCTGGGAGCAGACGTTACACACCCCCCGGCTGGGGACGGGAAGAAACC ATCTATCACAGCAGTGGTAGGCAGTATGGATGCACACCCCAGCCGATACTGTGCCACTGTGCGTGTACAG CGTCCACGGCAGGAGATCATTGAGGACTTGTCCTATATGGTGCGGGAGCTGCTCATCCAGTTCTACAAGT CCACCCGATTCAAGCCCACCCGAATCATCTTCTACCGAGATGGGGTCCCTGAAGGCCAGCTGCCTCAGAT CCTTCACTATGAGCTGCTTGCCATTCGAGATGCCTGCATCAAACTGGAAAGAGACTACCAGCCTGGGATC ACGTACATCGTGGTACAGAAGCGACATCACACCCGCCTCTTTTGTGCTGACAAGAATGAGCGGATTGGGA AGAGTGGTAACATCCCAGCTGGGACCACTGTGGACACCAACATCACTCACCCGTTTGAGTTTGACTTCTA CCTGTGCAGCCATGCAGGCATCCAGGGCACCAGCCGACCATCCCATTATTATGTCCTTTGGGATGACAAC CGTTTCACAGCAGATGAGCTCCAGATCTTGACATACCAGCTGTGCCACACTTATGTACGATGCACACGTT CTGTCTCTATCCCAGCACCTGCCTACTATGCCCGCCTGGTGGCTTTCCGGGCCCGATACCACCTGGTGGA CAAGGAGCATGACAGTGGAGAGGGGAGCCACATATCTGGGCAGAGCAATGGGCGAGACCCCCAGGCCCTG GCCAAAGCCGTGCAGGTTCACCAGGATACTCTACGCACCATGTACTTCGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR217268 representing NM_001191765
Red=Cloning site Green=Tags(s) MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNR EVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRM LHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWK MMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVC NVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRC IKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGG RNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFC KYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSN LCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQ RPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLERDYQPGI TYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDN RFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQAL AKAVQVHQDTLRTMYFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001191765 |
ORF Size | 2571 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001191765.2, NP_001178694.1 |
RefSeq Size | 7378 bp |
RefSeq ORF | 2574 bp |
Locus ID | 313594 |
MW | 97.2 kDa |
Gene Summary | This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to a C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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An miRNA-mediated therapy for SCA6 blocks IRES-driven translation of the CACNA1A second cistron
,null,
Science translational medicine
,PubMed ID 27412786
[Ago1]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN217268 | Ago1 (untagged) - Rat argonaute RISC catalytic component 1 (Ago1) |
CNY 8,270.00 |