Ago1 (NM_001191765) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Eif2c1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217268 representing NM_001191765 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCGGGACCCTCGGGAGCAGCTGCAGGTGCCTACCTGCCTCCCCTGCAGCAGGTGTTCCAGGCCC CTCGCCGGCCTGGCATCGGCACTGTGGGCAAACCGATCAAGCTTCTGGCCAATTACTTTGAGGTGGACAT TCCTAAGATTGACGTCTACCATTACGAGGTGGACATCAAGCCGGATAAGTGTCCTCGCAGAGTCAACCGG GAGGTGGTGGAGTACATGGTCCAGCATTTCAAACCGCAGATCTTTGGGGATCGCAAGCCTGTGTACGATG GAAAGAAGAACATTTACACAGTTACAGCACTGCCTATTGGCAATGAGAGGGTTGACTTTGAGGTGACCAT CCCTGGGGAAGGGAAGGATAGAATTTTTAAGGTCTCCATCAAGTGGCTAGCCATCGTGAGCTGGCGCATG CTGCATGAAGCCTTGGTCAGTGGCCAGATCCCTGTGCCCTTGGAGTCCGTGCAAGCCCTGGATGTGGCCA TGAGGCATCTGGCATCTATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTA CCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAG ATGATGCTCAACATTGATGTCTCAGCCACTGCCTTCTACAAAGCACAGCCAGTGATTGAGTTCATGTGTG AGGTCCTGGACATCAGGAACATAGATGAACAGCCCAAGCCCCTCACGGATTCCCAGCGCGTTCGGTTTAC CAAGGAGATAAAGGGCCTGAAGGTGGAAGTGACCCACTGTGGACAGATGAAGAGGAAATACCGCGTGTGT AATGTTACCCGTCGCCCTGCTAGCCATCAGACGTTTCCCTTGCAGCTGGAGAGCGGACAGACCGTGGAGT GCACCGTGGCACAATATTTCAAGCAGAAATACAACCTTCAGCTCAAGTATCCTCACCTGCCCTGCCTGCA AGTTGGGCAAGAGCAGAAGCATACCTATTTGCCCCTCGAGGTCTGTAACATTGTGGCTGGGCAGCGGTGC ATTAAGAAGCTGACTGACAACCAGACTTCAACCATGATAAAGGCTACAGCTAGGTCGGCCCCAGACAGAC AGGAGGAGATCAGTCGCCTGATGAAGAACGCCAGCTACAACTTGGATCCCTACATCCAGGAATTTGGAAT CAAAGTGAAGGATGACATGACGGAGGTGACGGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCGGC CGGAACCGGGCCATTGCTACACCCAACCAAGGTGTCTGGGACATGCGTGGGAAACAGTTCTACAATGGGA TTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAAAAACAATGTCGAGAAGAGGTGCTCAAGAA CTTCACGGACCAGCTTCGGAAGATCTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGTTTCTGC AAATACGCACAGGGGGCGGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAATACCTACTCAGGACTGC AGCTCATTATTGTCATCCTGCCCGGGAAGACGCCGGTGTATGCTGAAGTGAAGCGTGTTGGAGATACGCT CTTGGGAATGGCGACACAGTGTGTGCAGGTGAAGAATGTGGTCAAGACCTCACCTCAGACTCTGTCCAAC CTCTGCCTCAAGATCAATGTCAAACTCGGTGGCATCAACAACATCCTTGTCCCACACCAGCGCTCTGCTG TTTTCCAACAGCCAGTGATTTTTCTGGGAGCAGACGTTACACACCCCCCGGCTGGGGACGGGAAGAAACC ATCTATCACAGCAGTGGTAGGCAGTATGGATGCACACCCCAGCCGATACTGTGCCACTGTGCGTGTACAG CGTCCACGGCAGGAGATCATTGAGGACTTGTCCTATATGGTGCGGGAGCTGCTCATCCAGTTCTACAAGT CCACCCGATTCAAGCCCACCCGAATCATCTTCTACCGAGATGGGGTCCCTGAAGGCCAGCTGCCTCAGAT CCTTCACTATGAGCTGCTTGCCATTCGAGATGCCTGCATCAAACTGGAAAGAGACTACCAGCCTGGGATC ACGTACATCGTGGTACAGAAGCGACATCACACCCGCCTCTTTTGTGCTGACAAGAATGAGCGGATTGGGA AGAGTGGTAACATCCCAGCTGGGACCACTGTGGACACCAACATCACTCACCCGTTTGAGTTTGACTTCTA CCTGTGCAGCCATGCAGGCATCCAGGGCACCAGCCGACCATCCCATTATTATGTCCTTTGGGATGACAAC CGTTTCACAGCAGATGAGCTCCAGATCTTGACATACCAGCTGTGCCACACTTATGTACGATGCACACGTT CTGTCTCTATCCCAGCACCTGCCTACTATGCCCGCCTGGTGGCTTTCCGGGCCCGATACCACCTGGTGGA CAAGGAGCATGACAGTGGAGAGGGGAGCCACATATCTGGGCAGAGCAATGGGCGAGACCCCCAGGCCCTG GCCAAAGCCGTGCAGGTTCACCAGGATACTCTACGCACCATGTACTTCGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191765
Insert Size	2574 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191765.2, NP_001178694.1
RefSeq Size	7378 bp
RefSeq ORF	2574 bp
Locus ID	313594
Gene Summary	This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to a C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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