Smpd4 (NM_001167806) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1310674
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217236 representing NM_001167806 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCCCTCACCTACAACAGCCTAGTTTCCTTCTGGCTAGCCTGAAAGCTGACTCTATAAATAAGC CCTTTGCGCAGCGTTGCCAAGATTTGGTGAAAGTCATTGAGGATTTTCCAGCCAAGGAATTGCATGCCAT CTTCCCATGGCTGGTGGAGAGCATCTTTGGCAGCCTGGATGGTGTCCTTGTTGGCTGGAATCTCCGATGC CTACAGGGACGTGTGAACCCTGTGGAGTATAGCATAGCAATGGAATTTCTAGACCCAAGTGGCCCAATGA TGAAGTTGGTGTATAAGCTTCAAGCTGAAGACTATAACTTTGACTTCCCTGTCTCCTGTCTTCCTGGCCC TGTGAAGGCATCCATTCAAGAGAATGTCCTCCCTGACAGTCCTCTGTACCACAATAAGGTCCAGTTTCCC CCGACTGGAGGCCTGGGCCTGAATCTGGCCCTCAATCCATTCGAATACTATATGTTCTACTTTGCTTTGA GCCTCATCTCTCAAAAGCCAATGCCTATGTCTTTCCATGTCCGTACTTCGGACTGCGCCTATTTCATCCT GGTGGACAGGTACCTGTCGTGGTTCCTACCCACTGAAGGCAGCGTGCCTCCACCACTCTGCTCCAGTCCC GGGGGCTCCAGCCCCTCACCAGCTCCCAGGACACCAGCCATGCCCTTTGCCTCCTATGGCCTCCACACTA GCCTCTTGAAGCGGCACATCTCTCATCAGACATCTGTGAATGCAGACCCTGCATCCCATGAGATCTGGCG GTCAGAAACTCTACTCCAGGTTTTTGTGGAAATGTGGCTTCATCATTACTCCCTGGAGATGTACCAAAAA ATGCAGTCCCCTCATGCCAAGTTGGAGGTTCTACACTACCGACTCAATGTGTCCAGCGCCCTCCACAGCC CTGCCCAACCCAGCCTCCAGGCCCTCCACGCCTATCAAGAGTCCTTCATGCCTACTGAAGAGCATGTGCT GGTCGTGCGCCTGCTGCTGAAGCACCTGCATGCCTTTGCCAACAGCCTGAAGCCAGATCAGGCTTCACCA TCAGCACACTCCCATGCCACCAGTCCCCTGGAGGAATTCAAACGAGCTGCAGTACCTCGGTTTGTCCAGC AGAAGCTCTATGTCTTCCTGCAGCATTGTTTTGGCCACTGGCCTCTGGATGCATCCTTCAGAGCCGTCTT GGAGATGTGGCTGAGCTACCTGCAGCCATGGCGGTATGCCCCTGAGAAACAGGCTCAGGGCAGTGATTCC CAGCCACGGTGTGTGTCGGAGAGGTGGGCACCCTTCATCCAGGAAAATCTGCTAATGTACACCAAGCTGT TTGTGAGCTTCCTGAACCGTGCCCTACGCACAGACCTCGTCAGCCCCAAGAATGCACTCATGGTCTTCCG AGTGGCTAAGGTCTTTGCCCAGCCCAACCTGACTGAGATGATCCAGAAAGGTGAGCAGCTGTTCCTGGAG CCAGAGCTCATCATTCCCCACCGCCAACATCGGCTCTTCACAGCCACCAGCAGCTTCCTGTCACCATGGC CCCCTGTTGTGACAGATGCCTCGTTCAAGGTGAAGAGCCATGTCTACAGCCTGGAGGGCCAAGACTGCAA ATATACCCCAATGTTTGGTCCTGAAGTCCGAACCCTGGTCTTGCGCCTTGCTCAGCTCATCACACAGGCC AAGCAGACTGCCAAGTCCATCTCTGATCAGTGTGTGGAGAGCCCAACTGGCCGCTCCTTCCTGTCATGGC TGGGTTTTGGCTCCACAGACACAAATAGCTGGTACCCAGCCAATGACCCGGATGAAATGGGTCAGGACAG CATCCGCAAGACAGACGAGTACCTAGAGAAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAA GCCCAGCTGGCCCAGCTCACACTCGCCCTGGGGAATGCTCGGGACGAGAATGGGAAGCAGCAGCTCCCAG ACTGTATTGTGGGGGAAGAAGGACTCATTCTCACACCCTTGGGCCGATACCAGATCATCAATGGTCTGCG AAGGTTCGAGATTGAGTACCAGGGGGACTTAGAGCTGCAGCCCATCCGGAGCTATGAAATCACTAGTCTG GTCCGTGCACTCTTCCGGCTGTCTTCTGCCATCAACCGCAGATGTGCAGGACAGATGGCAGCTCTGTGCT CCCGGAATGACTTCCTTGGCAGCTTCTGTCGCTACCACCTCACTGAGCCTGCCTTGAGCAACAGACACCT GCTGAGCCCTGTGGGGCGGAGGCAGGTCACCACCCCTGCACGGGGCCCCAGACTCAGCCTCCGTTTCCTG GGCAGTTACCGGACACTGCTCCTGCTCCTGATGGCCTTCTTTGTGGCTTCTCTGTTCTGCATCGGGCCCC TGCCCTGCTCCTTGCTCCTGGTCCTAGGGTATATCCTCTACGCCATAGCTATGACACTGCTCACTGAGCG GGGGAAGCTGCACCAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217236 representing NM_001167806 Red=Cloning site Green=Tags(s) MAFPHLQQPSFLLASLKADSINKPFAQRCQDLVKVIEDFPAKELHAIFPWLVESIFGSLDGVLVGWNLRC LQGRVNPVEYSIAMEFLDPSGPMMKLVYKLQAEDYNFDFPVSCLPGPVKASIQENVLPDSPLYHNKVQFP PTGGLGLNLALNPFEYYMFYFALSLISQKPMPMSFHVRTSDCAYFILVDRYLSWFLPTEGSVPPPLCSSP GGSSPSPAPRTPAMPFASYGLHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQK MQSPHAKLEVLHYRLNVSSALHSPAQPSLQALHAYQESFMPTEEHVLVVRLLLKHLHAFANSLKPDQASP SAHSHATSPLEEFKRAAVPRFVQQKLYVFLQHCFGHWPLDASFRAVLEMWLSYLQPWRYAPEKQAQGSDS QPRCVSERWAPFIQENLLMYTKLFVSFLNRALRTDLVSPKNALMVFRVAKVFAQPNLTEMIQKGEQLFLE PELIIPHRQHRLFTATSSFLSPWPPVVTDASFKVKSHVYSLEGQDCKYTPMFGPEVRTLVLRLAQLITQA KQTAKSISDQCVESPTGRSFLSWLGFGSTDTNSWYPANDPDEMGQDSIRKTDEYLEKALEYLRQIFRLSE AQLAQLTLALGNARDENGKQQLPDCIVGEEGLILTPLGRYQIINGLRRFEIEYQGDLELQPIRSYEITSL VRALFRLSSAINRRCAGQMAALCSRNDFLGSFCRYHLTEPALSNRHLLSPVGRRQVTTPARGPRLSLRFL GSYRTLLLLLMAFFVASLFCIGPLPCSLLLVLGYILYAIAMTLLTERGKLHQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001167806
ORF Size	2469 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001167806.2, NP_001161278.2
RefSeq Size	3450 bp
RefSeq ORF	2472 bp
Locus ID	303790
MW	93.3 kDa

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