Lonrf3 (NM_001191585) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1565451
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217175 representing NM_001191585 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCCTTCAGACCGCACAGATGGTGAGCTTGTCCGCCGAGCTCGGCAGCAACAACTTGGAGCTGG CGGAGCCGGAGGAGCCGGCGGCATCGATGGCCGTAGGAGAGTCAGCCGCCCACTCGGAGAAGGTGACACC CGAGGGCTCCCAACCTCCCGGGGCTCAAGAGCCCGAGCAAAGCCAGCCTCTGGCGGCCTCGACGCCGCCG GAGTGCAAAGTCCTGCTCACGCAGGCGGATGCCTTGGCATCCCGGGGGCACCTTCGGGAAGCCCTCGAGG TGTACCGACAGCTCTCCGAGAGGCAGCAGCTCGTGGCGGAGCAGCTGGAGCAGCTAGTTCGCTGCCTGGC CGACAGTATCCCGCAAGACGAGCTGATGGCGCCAGCTCCTGCAGACCCGAGCGCCACTTCGAGCTGCTGC GCAGCGGCGCTGAAAAAGGCAGGGGAGGCCGCGGCCGTGGCCCCCGAGGTATGGGATGGCTTTAAGTGCA GGAAGTGTCACGGGTTCCTCTCCGACCCGGTGTCCCTGTGGTGCGGCCACACCTTTTGTAAACTGTGCCT GGAGCGCGGGCGAGCCGCCGATAGGCGCTGCGCTCTGTGCGGGGTCAAGCTCTCCGCACTCATGGTGGCC AGTGGGAGGGCACGCGGGCCGCGGCGCTCGGGGCCGCAGGCGCCACTGCAACTGCGAGTCAACGTGGTAC TCAGCGGTCTCCTGGGCAAGTTGTTCCCGGGCCCCGCGCGAGCGTCGCAACTCCGGCACGAGGGCAACCG GCTGTACCGTGAGCGCCAGGTGGAGGCGGCTCTGCTCAAGTACAACGAGGCTGTTCGTCTAGCTCCAAAT GACCATTTGCTTTATAGCAATCGGTCCCAAATTTATTTCACCTTGGAGTCTCATGAGGATGCACTGCATG ATGCAGAAATAGCATGTAAACTACGGCCAATGGGTTTTAAGGCACACTTCAGAAAAGCACAAGCTTTAGC CACCCTAGGCAAGGTGAAAGAAGCACTCAGAGAATTTCTCTACTGTGTATCCCTTGATGGAAAGAACAAG AGAGCCAGATCTGAAGCGCAAAGGGAGAATTCAGAGCTTCCCCATTGTTCTAATCAGGAGGGAGCAGCAG CTAGGGAAGAAAGCAGCAGCCTAGTGAACATGGCTCAAGGAAAGGTGAGTAGAAAAGAAGACAGAGCGAA AAGTCAGGAAGAAGAGGAACAAGATGCTGCCTCCGTCAGGACCGGCAAATGCCTGGAAAAGAAGAGGAAT CGTTGCCAAATTGAAACCCAAGAAGACACAGAATTGCCTAATAAAGTCTCCAAACAAGATCTTCCTGCTG AGCAGGGGGCCAAACCGGATCTCAGTATTCCACTTGGATCTTTTGATGCATCTGACCTTGAATGCTCACT GTGTATGAGATTGTTCTATGAGCCAGTCACAACACCTTGTGGGCACACCTTTTGCTTGAAATGTCTTGAG AGATGCCTGGATCATAATGCAAAGTGTCCACTGTGCAAAGACGTTCTTTTACAGTGCTTGCCATCAAGAA AATACAGCAAGAATGTAATAATGGAAGAACTCATAGCTAAGTTCCTACCAGAAGAACTCAAGGAACGAAG AAGGCTTTATGAAGAAGAAATGGAAGAGCTTTCTAACTTAAATAAGAATGTGCCCATTTTCGTGTGTACC ATGGCCTATCCCACCGTTCCTTGTCCCTTGCACATCTTTGAGCCTTGTTACCGCCTGATGATTCGAAGAT GTATTGAGACAGGCACAAAACAGTTTGGCATGTGCCTTGGAGATCCTGTCAAAGGGTTTGCAGAATATGG CTGCATCTTGGAGATCAGAAATGTCCAATTCTTTTCTGACGGCCGATCAGTGGTTGACAGCATAGGCAAG AGGCGCTTCAAAGTGCTCCATCAGGGTCAACGGGATGGCTACAACACTGCTGACATTGAATACATTGAAG ATCAAAAGGTTCAGGGAGACGATTGTGCTGAACTCGTGGGATTGCATAACTGTGTGTACGAGCAAGCGTC ATCATGGTTTCATTCACTCAAAACATCGCTGAAGAATCGGATACTAAATCACTTTGGCCCAATGCCAGAG AAGGATGAAGACCCCCAGGTCAATCCGAATGGTCCAGCCTGGTGCTGGTGGACACTGGCGGTTCTTCCCC TGGAAAGCAGAGCTCAGCTTCCCTTCCTAGCAATGAGGTCCTTAAAGGATAGACTGAATGGTATCCGGCG TATCCTGGCCTTTATATCGAGAAACCAAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217175 representing NM_001191585 Red=Cloning site Green=Tags(s) MDSLQTAQMVSLSAELGSNNLELAEPEEPAASMAVGESAAHSEKVTPEGSQPPGAQEPEQSQPLAASTPP ECKVLLTQADALASRGHLREALEVYRQLSERQQLVAEQLEQLVRCLADSIPQDELMAPAPADPSATSSCC AAALKKAGEAAAVAPEVWDGFKCRKCHGFLSDPVSLWCGHTFCKLCLERGRAADRRCALCGVKLSALMVA SGRARGPRRSGPQAPLQLRVNVVLSGLLGKLFPGPARASQLRHEGNRLYRERQVEAALLKYNEAVRLAPN DHLLYSNRSQIYFTLESHEDALHDAEIACKLRPMGFKAHFRKAQALATLGKVKEALREFLYCVSLDGKNK RARSEAQRENSELPHCSNQEGAAAREESSSLVNMAQGKVSRKEDRAKSQEEEEQDAASVRTGKCLEKKRN RCQIETQEDTELPNKVSKQDLPAEQGAKPDLSIPLGSFDASDLECSLCMRLFYEPVTTPCGHTFCLKCLE RCLDHNAKCPLCKDVLLQCLPSRKYSKNVIMEELIAKFLPEELKERRRLYEEEMEELSNLNKNVPIFVCT MAYPTVPCPLHIFEPCYRLMIRRCIETGTKQFGMCLGDPVKGFAEYGCILEIRNVQFFSDGRSVVDSIGK RRFKVLHQGQRDGYNTADIEYIEDQKVQGDDCAELVGLHNCVYEQASSWFHSLKTSLKNRILNHFGPMPE KDEDPQVNPNGPAWCWWTLAVLPLESRAQLPFLAMRSLKDRLNGIRRILAFISRNQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191585
ORF Size	2271 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191585.1, NP_001178514.1
RefSeq Size	2711 bp
RefSeq ORF	2274 bp
Locus ID	298322
MW	84.4 kDa

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