Dclk2 (NM_001195832) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CL2; CLICK-II; CLICK2; Dck2; RGD1308384
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217138 representing NM_001195832 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCACCAGGAGCATTGAGCTGGAACATTTTGAAGAACGGGACAAAAGGCCGCGGCCAGGGTCGC GGAGAGGAGCTCCCAGCTCCTCCGGGGGCAGCAGCATCTCTGGCCCCAAGGGCAACGGGCTCATCCCCAG CCCGGCGCACAGTGCTCACTGCAGCTTCTACCGCACGCGGACCTTGCAGGCCCTCAGCTCGGAGAAGAAG GCCAAGAAGGCGCGGTTCTACCGGAATGGGGACCGCTACTTCAAAGGCCTGGTCTTTGCGATCTCCAGCG ACCGTTTCCGTTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCGCTGTCTGACAATGTGAACCTGCC CCAGGGCGTCCGCACAATCTACACCGTAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA GGTGAAAGTTACGTGTGTGCCTCCAACGAACCATTCCGTAAAGTTGACTACACCAAAAACGTTAACCCGA ACTGGTCTGTGAACATCAAGGGTGGTACTACCCGCACCTTGGCCGTGGCCTCGGCCAAGAGTGAGGTGAA AGAAAGCAAGGACTTCATCAAACCCAAGCTCGTGACTGTGATTCGAAGTGGGGTGAAGCCTAGAAAGGCC GTGCGCATCCTTCTGAATAAAAAGACTGCCCATTCCTTTGAGCAGGTCCTAACGGATATCACCGAAGCCA TTAAACTAGACTCCGGCGTGGTGAAGAGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGA CTTTTTTGGAGACGATGATGTTTTTATTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTT GTCCTGGATCATAGTGAGTGCCGTGTCCTGAAGTCATCTTATTCTCGGGCCTCAGCTGCTAAGTATTCTG GATCCAGAAGCCCAGGGCTCTCCCGCCGCAGCAAGTCACCAGCTTCAGTAAAGAGGGCTGGCCACTCCAG TGCCTATTCTACAGCCAAATCCCCAGTGAATGGAACTCCCAGCAGCCAGCTTTCCACTCCTAAGTCCACC AAGTCCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGACTGAAGCAGATTTCTGCTCAGGGCA GATCTTCTTCCAATGTAAACGGTGGGCCCGAACTTGACCGTTGCATGAGTCCTGAAGGCGTGAATGGAAA CAGGTGCTCCGAGTCATTCACTCTTCTGGAGAAATACAGAATCGGGAAGGTCATCGGAGACGGCAACTTT GCCGTAGTTAAAGAATGTATGGACAGGTCCACTGGAAAGGAGTTTGCATTAAAGATTATTGACAAAGCCA AATGCTGTGGAAAGGAACATCTGATTGAGAACGAAGTGTCAATCCTGCGCCGGGTGAAGCACCCCAACAT CATCATGCTGGTTGAGGAGATGGAAACAACAACTGAGCTCTTCCTAGTGATGGAACTGGTCAAAGGTGGA GATCTCTTCGACGCGATCACTTCTTCAACCAAGTACACCGAGAGAGATGGCAGTGCCATGGTGTACAACC TAGCCAGCGCCCTCAGATACCTGCACGGCCTCAGCATCGTGCACAGAGATATCAAACCTGAGAATCTGCT GGTGTGCGAATACCCAGACGGAACCAAGTCGCTGAAGCTGGGAGACTTTGGGCTGGCGACGGTGGTTGAA GGCCCGTTGTACACAGTCTGTGGCACCCCAACTTACGTGGCACCCGAGATCATTGCTGAAACAGGTTACG GCCTGAAGGTGGATGTTTGGGCAGCTGGTGTGATTACGTACATTCTTCTCTGTGGATTCCCACCGTTCCG GAGTGAGAACAACCTCCAGGAGGATCTCTTTGACCAGATCTTGGCCGGAAAGCTGGAGTTTCCAGCTCCC TACTGGGACAACATTACAGACTCTGCCAAGGAGTTAATCAGTCAAATGCTTCAGGTAAACGTTGAAGCTC GCTGTACTGCGGGAGAAATTCTGAGTCACCCCTGGGTGTCAGATGATGCATCCCAGGAGAATAATATGCA AGCCGAGGTTACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACCACCACT GGGGTCTCCGTTATCATGGTCCAAGGCCATGAACACGGCTCTAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217138 representing NM_001195832 Red=Cloning site Green=Tags(s) MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKK AKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTVDGSRKVTSLDELLE GESYVCASNEPFRKVDYTKNVNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKA VRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDDF VLDHSECRVLKSSYSRASAAKYSGSRSPGLSRRSKSPASVKRAGHSSAYSTAKSPVNGTPSSQLSTPKST KSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGPELDRCMSPEGVNGNRCSESFTLLEKYRIGKVIGDGNF AVVKECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETTTELFLVMELVKGG DLFDAITSSTKYTERDGSAMVYNLASALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVE GPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAP YWDNITDSAKELISQMLQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTT GVSVIMVQGHEHGSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001195832
ORF Size	2145 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001195832.1, NP_001182761.1
RefSeq Size	3982 bp
RefSeq ORF	2148 bp
Locus ID	310698
UniProt ID	Q5MPA9
MW	78.4 kDa
Gene Summary	This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that this gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]

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