Prkcq (NM_001276721) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Pkcq
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217128 representing NM_001276721 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCGTTTCTTCGAATTGGTTTATCCAACTTTGACTGTGGGACCTGTCAAGCTTGTCAGGGAGAGG CGGTGAACCCCTACTGTGCCGTGCTTGTCAAAGAGTATGTGGAATCAGAAAACGGGCAGATGTACATCCA GAAAAAGCCGACCATGTACCCGCCTTGGGACAGCACCTTTGATGCCCACATTAACAAGGGAAGGGTGATG CAGATCATCGTGAAAGGCAAAAATGTAGACCTCATATCAGAAACCACCGTGGAGCTCTACTCCCTGGCAG AGAGATGCCGCAAGAACAATGGGCGGACAGAAATATGGTTAGAGCTGAAACCTCAAGGCCGAATGCTAAT GAATGCAAGATACTTTCTGGAAATGAGTGACACAAAGGACATGAGTGAATTTGAGAACGAAGGATTCTTT GCACTGCATCACCGCCGAGGCGCCATCAAACAAGCCAAAGTCCACCACGTCAAGTGTCACGAGTTCACAG CCACCTTTTTCCCTCAACCCACGTTCTGCTCTGTCTGCCATGAATTTGTCTGGGGGCTGAACAAGCAGGG CTACCAGTGCCGACAATGTAATGCAGCGATTCACAAGAAGTGCATCGATAAAGTGATAGCCAAGTGCACA GGATCGGCGATCAATAGTCGAGAGACCATGTTCCACAAGGAGAGATTCAAGATCGACATGCCACACAGAT TCAAAGTCTACAACTACAAGAGTCCAACCTTCTGTGAGCACTGTGGTACCCTGCTATGGGGGCTGGCGAG GCAAGGTCTCAAGTGTGATGCATGTGGCATGAACGTCCACCACCGATGCCAGACAAAGGTTGCCAACCTC TGTGGTATAAACCAGAAGCTAATGGCTGAAGCGCTAGCAATGATTGAAAGCACTCAACAGGCTCGCACCT TAAGAGATTCAGAACACATCTTCCGAGAAGGACCAATTGAAATCAGTTTCCCGCGCTCCATCAAAAGTGA AACCAGGCCACCATGCGTACCAACACCTGGGAAAAAAGAACCCCAGGGAATTTGCTGGGAGTCCCCTTTG GATGGGGCAGATAAAACGGCCCAGCCTCCTGAACCTGAAGTGAACTTGCAAAGGGCTTCTCTGCAACTGA AACTGAAGATCGATGACTTCATCCTGCACAAGATGCTGGGGAAAGGAAGTTTTGGCAAGGTCTTCCTGGC AGAGTTCAAGAGAACCAAACAGTTTTTCGCAATAAAAGCCTTAAAGAAAGATGTGGTGTTGATGGACGAT GACGTCGAGTGCACGATGGTGGAGAAGAGAGTTCTGTCCTTGGCCTGGGAGCATCCGTTTCTTACACACA TGTTCTGCACATTCCAGACCAAGGAAAATCTCTTTTTCGTGATGGAGTATCTCAATGGAGGAGACTTAAT GTACCACATCCAAAGTTGCCACAAATTTGATCTTTCCAGAGCCACGTTTTATGCTGCTGAGATCATCCTT GGTCTACAGTTCCTTCATTCCAAAGGAATTGTCTACAGGGACCTGAAGCTAGATAATATCCTGTTAGACA GAGATGGCCATATCAAAATAGCAGACTTTGGGATGTGCAAAGAGAATATGCTGGGAGATGCGAAGACAAA TACTTTCTGTGGGACGCCTGACTACATCGCTCCGGAGATCTTGCTGGGTCAGAAATACAACCATTCCGTT GACTGGTGGTCCTTTGGGGTTCTTCTTTACGAGATGCTGATTGGCCAGTCGCCTTTCCACGGGCAGGACG AAGAGGAGCTTTTCCACTCCATCCGCATGGACAATCCCTTTTACCCAAGGTGGCTAGAAAGGGAGGCCAA GGATCTTCTAGTGAAGCTTTTTGTGAGAGAGCCTGAAAAGAGGCTGGGAGTGAGAGGAGACATCCGCCAG CATCCTTTGTTTCGAGAGATCAACTGGGAAGAGCTTGAGAGAAAAGAGATTGACCCACCCTTCAGACCAA AAGTGAAATCACCATATGACTGTAGCAATTTCGACAAGGAATTCCTAAGTGAGAAACCCCGGCTATCGTT CGCTGACAGAGCACTCATCAACAGCATGGACCAGAACATGTTCAGCAACTTCTCCTTCATTAACCCGGGG ATGGAGACTCTCATTTGCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217128 representing NM_001276721 Red=Cloning site Green=Tags(s) MSPFLRIGLSNFDCGTCQACQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVM QIIVKGKNVDLISETTVELYSLAERCRKNNGRTEIWLELKPQGRMLMNARYFLEMSDTKDMSEFENEGFF ALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCT GSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANL CGINQKLMAEALAMIESTQQARTLRDSEHIFREGPIEISFPRSIKSETRPPCVPTPGKKEPQGICWESPL DGADKTAQPPEPEVNLQRASLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTKQFFAIKALKKDVVLMDD DVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEIIL GLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSV DWWSFGVLLYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQ HPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPG METLICS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276721
ORF Size	2121 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276721.1, NP_001263650.1
RefSeq Size	3370 bp
RefSeq ORF	2124 bp
Locus ID	85420
MW	81.8 kDa
Gene Summary	mediates the integration of TCR- and CD28-derived signals leading to NFk-B transcription factor activation necessary for efficient IL-2 production and T cell proliferation [RGD, Feb 2006]

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