Mapk9 (NM_001270544) Rat Tagged ORF Clone
CAT#: RR216633
- TrueORF®
Mapk9 (myc-DDK-tagged) - Rat mitogen-activated protein kinase 9 (Mapk9), transcript variant 2
"NM_001270544" in other vectors (1)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SAPK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR216633 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACAGTAAAAGCGATGGCCAGTTTTACAGTGTGCAAGTGGCAGACTCAACTTTCACTGTTCTAA AACGTTACCAGCAGTTGAAACCAATTGGCTCTGGAGCCCAAGGAATTGTTTGTGCTGCTTTTGATACAGT TCTTGGAATAAATGTTGCTGTCAAGAAGTTAAGTCGTCCTTTTCAGAACCAAACGCATGCAAAGAGAGCC TACCGTGAACTTGTCCTCCTAAAGTGTGTCAATCATAAAAATATAATTAGCTTGTTAAATGTGTTCACAC CACAAAAAACGCTAGAAGAATTCCAAGATGTGTACTTGGTTATGGAGTTAATGGACGCTAACTTATGTCA GGTTATTCATATGGAGCTGGACCATGAAAGAATGTCATACCTCCTCTACCAGATGCTTTGTGGCATTAAG CACCTGCATTCAGCTGGCATAATTCATAGGGATTTGAAGCCTAGCAACATTGTAGTAAAATCAGACTGTA CTCTCAAGATCCTTGACTTTGGCCTGGCACGGACAGCCTGTACCAACTTTATGATGACTCCCTATGTGGT AACTCGCTACTATCGGGCTCCAGAAGTCATCCTGGGCATGGGCTACAAGGAGAATGTGGACATCTGGTCT GTCGGGTGCATCATGGCAGAAATGGTCCTCCATAAAGTCCTGTTCCCAGGAAGAGACTATATTGATCAAT GGAATAAAGTTATTGAACAGCTAGGAACACCATCCGCAGAGTTCATGAAGAAACTTCAGCCAACTGTAAG GAATTATGTGGAAAACAGACCAAAGTACCCTGGAATCAAATTTGAAGAGCTCTTTCCAGATTGGATATTT CCGTCAGAATCCGAACGAGACAAAATAAAAACAAGTCAAGCCAGAGATCTGTTATCGAAAATGTTAGTGA TTGATCCGGACAAGCGGATCTCTGTGGACGAAGCCTTGCGCCACCCGTATATTACTGTTTGGTATGACCC CGCTGAAGCAGAAGCGCCACCACCTCAAATTTATGATGCCCAGTTGGAAGAAAGAGAGCATGCGATTGAA GAGTGGAAAGAACTAATTTACAAAGAAGTGATGGACTGGGAAGAAAGAAGCAAGAATGGGGTGAAAGACC AGCCTTCAGATGCAGCAGTAAGCAGCAAGGCTACTCCTTCTCAGTCGTCATCCATCAATGACATCTCATC CATGTCCACTGAGCACACCCTGGCCTCAGACACAGACAGCAGTCTCGATGCCTCAACCGGACCCCTGGAA GGCTGCCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR216633 protein sequence
Red=Cloning site Green=Tags(s) MSDSKSDGQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRA YRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIK HLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWS VGCIMAEMVLHKVLFPGRDYIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIF PSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIE EWKELIYKEVMDWEERSKNGVKDQPSDAAVSSKATPSQSSSINDISSMSTEHTLASDTDSSLDASTGPLE GCR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001270544 |
ORF Size | 1269 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001270544.1, NP_001257473.1 |
RefSeq Size | 3711 bp |
RefSeq ORF | 1272 bp |
Locus ID | 50658 |
UniProt ID | P49186 |
MW | 48.2 kDa |
Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases are multifunctional proteins. They are involved in a wide variety of cellular processes such as growth, proliferation, differentiation, transcription regulation, and development. They function in stress responses, apoptosis, inflammation and transformation. For example, this kinase plays a dominant role in mediating proliferation of lung cancer and prostate cancer cells, and in regulation of osmotic stress-induced tight junction disruption. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN216633 | Mapk9 (untagged) - Rat mitogen-activated protein kinase 9 (Mapk9), transcript variant 2 |
CNY 3,990.00 |