Mapk9 (NM_001270544) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	SAPK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216633 representing NM_001270544 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACAGTAAAAGCGATGGCCAGTTTTACAGTGTGCAAGTGGCAGACTCAACTTTCACTGTTCTAA AACGTTACCAGCAGTTGAAACCAATTGGCTCTGGAGCCCAAGGAATTGTTTGTGCTGCTTTTGATACAGT TCTTGGAATAAATGTTGCTGTCAAGAAGTTAAGTCGTCCTTTTCAGAACCAAACGCATGCAAAGAGAGCC TACCGTGAACTTGTCCTCCTAAAGTGTGTCAATCATAAAAATATAATTAGCTTGTTAAATGTGTTCACAC CACAAAAAACGCTAGAAGAATTCCAAGATGTGTACTTGGTTATGGAGTTAATGGACGCTAACTTATGTCA GGTTATTCATATGGAGCTGGACCATGAAAGAATGTCATACCTCCTCTACCAGATGCTTTGTGGCATTAAG CACCTGCATTCAGCTGGCATAATTCATAGGGATTTGAAGCCTAGCAACATTGTAGTAAAATCAGACTGTA CTCTCAAGATCCTTGACTTTGGCCTGGCACGGACAGCCTGTACCAACTTTATGATGACTCCCTATGTGGT AACTCGCTACTATCGGGCTCCAGAAGTCATCCTGGGCATGGGCTACAAGGAGAATGTGGACATCTGGTCT GTCGGGTGCATCATGGCAGAAATGGTCCTCCATAAAGTCCTGTTCCCAGGAAGAGACTATATTGATCAAT GGAATAAAGTTATTGAACAGCTAGGAACACCATCCGCAGAGTTCATGAAGAAACTTCAGCCAACTGTAAG GAATTATGTGGAAAACAGACCAAAGTACCCTGGAATCAAATTTGAAGAGCTCTTTCCAGATTGGATATTT CCGTCAGAATCCGAACGAGACAAAATAAAAACAAGTCAAGCCAGAGATCTGTTATCGAAAATGTTAGTGA TTGATCCGGACAAGCGGATCTCTGTGGACGAAGCCTTGCGCCACCCGTATATTACTGTTTGGTATGACCC CGCTGAAGCAGAAGCGCCACCACCTCAAATTTATGATGCCCAGTTGGAAGAAAGAGAGCATGCGATTGAA GAGTGGAAAGAACTAATTTACAAAGAAGTGATGGACTGGGAAGAAAGAAGCAAGAATGGGGTGAAAGACC AGCCTTCAGATGCAGCAGTAAGCAGCAAGGCTACTCCTTCTCAGTCGTCATCCATCAATGACATCTCATC CATGTCCACTGAGCACACCCTGGCCTCAGACACAGACAGCAGTCTCGATGCCTCAACCGGACCCCTGGAA GGCTGCCGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270544
Insert Size	1272 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270544.1, NP_001257473.1
RefSeq Size	3711 bp
RefSeq ORF	1272 bp
Locus ID	50658
UniProt ID	P49186
Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases are multifunctional proteins. They are involved in a wide variety of cellular processes such as growth, proliferation, differentiation, transcription regulation, and development. They function in stress responses, apoptosis, inflammation and transformation. For example, this kinase plays a dominant role in mediating proliferation of lung cancer and prostate cancer cells, and in regulation of osmotic stress-induced tight junction disruption. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) has an alternate exon in the coding region, compared to variant 1. The resulting isoform (2) is of the same size, but has a different internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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