Rad54l2 (NM_001134520) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1561889
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR215273 representing NM_001134520 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGACGAATCCGCCTCAGGGAGCGATCCAGACCTGGACCCGGACGTGGAGCTGGAGGATGCGGAAG AGGAGGAGGAGGAGGAGGAGGTGGCAGTGGAGGAGCATGACAGGGATGACGAGGAAGGCCTGCTGGATGA CCCATCCCTGGAAGGCATGTGTGGCACTGAGCATGCCCAGCTGGGGGAAGATGGGCAGCGGCCGCCGCGG TGCACTTCAACTACCTCATCTCAGTCTGAGCCTTCAGAGCAGCTTAGGCACCAAGGCAAGATCTTAGCAT CTGAGGACCCCAAAAAGAAGCGAGCTCAGAAGCCCTCTCACATGAGAAGAAACATACGAAAGCTACTTCG GGAGGATCAGTTGGAGCCCGTTACCAAAGCAGCACAGCAGGAAGAATTGGAAAGAAGAAAGCGCCTGGAG CAGCAGAGGAAAGAATATGCAGCCCCTATTCCTACTGTCCCTTTGGAGTTCCTACCTGAGGAAATTGTCT TACGAGCTAGTGATGGTCCCCAGCTCCCTCCTCGGGTCTTGGCCCAGGAAGTCATTTGTTTGGACAGTAG CAGTGGCAGTGAGGATGAAAAAAGCAGTCGAGACGAGGTCATTGAACTGAGTTCTGGAGAGGAGGACACT CTGCACATTGTGGACAGCAGTGAGTCTGTCAGTGAGGAGGACGAGGAAGAGGAGAAGGGTGGCACCCATG TGAATGACGCCTTAAACCAGCATGACGCTCTTGGGCGGGTACTTGTCAACCTGAATCACCCTCCAGAGGA GGAGAATGTCTTCCTGGCCCCACAGTTGGCACGGGCTGTGAAACCTCATCAGATTGGTGGGATCCGGTTC CTATATGATAACCTAGTGGAGTCCTTAGAAAGGTTTAAGACCAGTAGTGGCTTTGGCTGTATCCTGGCCC ATAGCATGGGTCTGGGGAAAACTCTGCAAGTGATCTCCTTCATTGATGTCCTTTTCCGCCACACGCCAGC CAAAACAGTCCTTGCGATTGTACCGGTGAACACTCTTCAGAATTGGTTGGCAGAGTTCAACATGTGGCTC CCGGCTCCTGAAGCCCTCCCAGCCGACAGCAAGCCTGAAGAAATCCAGCCTCGGTTCTTTAAAGTTCATA TCTTGAATGATGAACACAAGACAGTGGCATCCCGTGCTAAAGTGACGACTGATTGGGTTTCAGAGGGTGG AGTGCTGCTGATGGGGTATGAGATGTACAGACTGCTCACCCTGAAGAAGTCCTTAGCCACAGGCAGACCC AAGAAAACCAAGAAACGGTCTCACCCGGTCATCATTGATCTGGATGAAGAAGATCGGCAGCAGGAGTTCC GGAGAGAGTTTGAGAAGGCCTTATGTCGCCCTGGTCCTGATGTGGTAATCTGTGACGAGGGACACCGCAT CAAAAATTGCCAAGCCAGCACCTCACAGGCTCTGAAGAACATACGTTCTCGTCGGCGGGTAGTGCTGACC GGCTACCCTCTACAGAACAACCTCATCGAGTACTGGTGCATGGTGGACTTTGTGCGCCCAGATTTCCTTG GTACTCGTCAGGAGTTCAGCAATATGTTTGAACGCCCTATCCTAAACGGACAGTGTATTGACAGCACACC TCAGGATGTCCGCCTCATGCGCTACCGGAGCCATGTTTTGCACAGCCTCCTAGAGGGCTTTGTGCAGAGG AGAGGCCACACTGTGTTGAAGATTCACCTCCCTGCCAAAGAAGAGAATGTAATCCTGGTGCGGCTTTCTC AGATCCAGCGGGATTTGTACACACAATTCATGGACCGTTTCCGGGATTGTGGTAGCAGTGGCTGGTTGGG GCTGAATCCTCTGAAGGCTTTCTGTGTATGCTGCAAGATCTGGAACCATCCTGATGTGCTATATGAAGCC CTTCAGAAGGAAAACCTAGCCAATGAGCAGGACCTAGATGTGGAAGAACTTGGCTCAGCAGGGACCAGTA CCCGCTGTCCACCACATGGCACAAAAGTCAAGGGAGAAGATAGTGCCTTGCCTTCCTCAATGGGAGAAGC AACCAATAGCAAGTTCCTACAGGGAGTTGGCTTTAATCCTTTCCAGGAACGGGGCAATAACATCGTTACA TATGAATGGGCCAAGGATCTTCTGACTAATTATCAGACTGGAGTCCTGGAAAACTCTCCCAAGATGGTAC TTCTTTTCCACCTGATTGAAGAGAGTGTGAAGCTTGGGGACAAGATTCTTGTATTTAGCCAGAGCCTTTC TACCCTGGCTCTCATCGAGGAGTTCCTAGGGAAACGAGACATGCCTTGTCCGCCTGGTGCCGAGGGGCAA GGAGCACAGAAGTGGGTTCGAAATGTCAGCTACTTCCGGCTAGACGGTAGCACCCCTGCCTTTGAGAGGG AGCGGCTCATTAATCAGTTCAATGATCCCAGCAACCTCACCACCTGGCTGTTCCTTCTCTCTACAAGGGC CGGATGCTTGGGTGTGAATCTGATTGGTGCCAATCGAGTGGTGGTATTTGATGCTTCCTGGAACCCTTGC CATGATGCCCAGGCAGTATGTCGGGTATACCGTTATGGCCAGAAAAAGCCCTGTTACATCTATCGACTCG TGGCTGATTATACTCTTGAAAAGAAGATCTATGACCGGCAGATTTCCAAGCAGGGCATGTCAGATCGGGT CGTGGATGATCTAAATCCAATGCTGAACTTCACCCGGAAGGAAGTGGAAAACCTGCTGCATTTTGTTGAG AAGGAGCCAGCTCCCCAAACATCTTTGGATATAAAGGGGATCAAGGAGTCAGTCTTGCAACTTGCCTGCC TGAAGTATCCTCACCTCATCACCAAGGAGCCTTTTGAGCACGAGTCACTGCTCCTCAACCGAAAAGATCA CAAGCTGACCAAAGCTGAGAAGAAAGCAGCAAAGAAAAGCTATGAGGAGGACAAACGCACATCAGTACCC TACACCCGCCCATCGTATGCGCAGTATTATCCTGCCAGCGACCAGAGCCTGACCAGCATCCCTGCCTTCA GTCAGAGGAACTGGCAGCCAACACTGAAGGGTGATGATAAGCCTGTGGCCAGCGTTCGTCCTGTACAGTC CACCCCCATCCCCATGATGCCCCGGCATGTCCCACTCGGTGGCAGTGTAAGCTCTGCCTCTAACACAAAT CCATCCATGAACTTCCCCATCAACTACTTGCAGCGGGCAGGAGTCCTTGTGCAGAAAGTGGTCACCACGA CAGATATTGTTATTCCTGGACTCAACAGCTCGACAGATGTTCAGGCAAGGATCAGTGCTGGTGAGAGCAT CCACATCATCCGGGGGACGAAAGGGACATACATCCGTACCAGTGATGGACGAATCTTTGCTGTCCGGGCA ACTGGCAAACCAAAGGCCCCTGAAGATGGTCGGATGGCTGCCTCAGGTTCCCAGGGGCCTTCTCTTGCAT CCACGAGCAATGGCAGACACAGTGCCTCATCACCCAAAGCCCCTGACCCTGAGGGGCTGGCCAGGCCCGT CTCTCCTGACAGCCCAGAAATCATCAGTGAGCTCCAGCAGTATGCAGATGTGGCCGCTGCTCGGGAATCC CATCAGAACTCCCCAAGCATCAGTGCTGCCCTGCCTGGCCCCCCGGCCCAACTTATGGACAATAGCACCA TTCCTGGGACAGCTCTTGGAACTGAGCCATGCCTTGGGGGTCATTGCCTCAATAGTTCCCTCTTGGTGAC TGGTCAGCCCAGTAGTGGCAGGCATCCAGTGCTGGACTTAAGGGGCCATAAGCGAAAGTTGGCCACTCCA TCTGTCTCCCAGGAATCAGTCCGTCGACGGTCCAGGAAGGGCCATCTGCCAGCCCCGTGCAGCCCTATGA ACACGGGTATCCAGTCTCTGGCGGGTTTTCAATGCCACCTGTCTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR215273 representing NM_001134520 Red=Cloning site Green=Tags(s) MSDESASGSDPDLDPDVELEDAEEEEEEEEVAVEEHDRDDEEGLLDDPSLEGMCGTEHAQLGEDGQRPPR CTSTTSSQSEPSEQLRHQGKILASEDPKKKRAQKPSHMRRNIRKLLREDQLEPVTKAAQQEELERRKRLE QQRKEYAAPIPTVPLEFLPEEIVLRASDGPQLPPRVLAQEVICLDSSSGSEDEKSSRDEVIELSSGEEDT LHIVDSSESVSEEDEEEEKGGTHVNDALNQHDALGRVLVNLNHPPEEENVFLAPQLARAVKPHQIGGIRF LYDNLVESLERFKTSSGFGCILAHSMGLGKTLQVISFIDVLFRHTPAKTVLAIVPVNTLQNWLAEFNMWL PAPEALPADSKPEEIQPRFFKVHILNDEHKTVASRAKVTTDWVSEGGVLLMGYEMYRLLTLKKSLATGRP KKTKKRSHPVIIDLDEEDRQQEFRREFEKALCRPGPDVVICDEGHRIKNCQASTSQALKNIRSRRRVVLT GYPLQNNLIEYWCMVDFVRPDFLGTRQEFSNMFERPILNGQCIDSTPQDVRLMRYRSHVLHSLLEGFVQR RGHTVLKIHLPAKEENVILVRLSQIQRDLYTQFMDRFRDCGSSGWLGLNPLKAFCVCCKIWNHPDVLYEA LQKENLANEQDLDVEELGSAGTSTRCPPHGTKVKGEDSALPSSMGEATNSKFLQGVGFNPFQERGNNIVT YEWAKDLLTNYQTGVLENSPKMVLLFHLIEESVKLGDKILVFSQSLSTLALIEEFLGKRDMPCPPGAEGQ GAQKWVRNVSYFRLDGSTPAFERERLINQFNDPSNLTTWLFLLSTRAGCLGVNLIGANRVVVFDASWNPC HDAQAVCRVYRYGQKKPCYIYRLVADYTLEKKIYDRQISKQGMSDRVVDDLNPMLNFTRKEVENLLHFVE KEPAPQTSLDIKGIKESVLQLACLKYPHLITKEPFEHESLLLNRKDHKLTKAEKKAAKKSYEEDKRTSVP YTRPSYAQYYPASDQSLTSIPAFSQRNWQPTLKGDDKPVASVRPVQSTPIPMMPRHVPLGGSVSSASNTN PSMNFPINYLQRAGVLVQKVVTTTDIVIPGLNSSTDVQARISAGESIHIIRGTKGTYIRTSDGRIFAVRA TGKPKAPEDGRMAASGSQGPSLASTSNGRHSASSPKAPDPEGLARPVSPDSPEIISELQQYADVAAARES HQNSPSISAALPGPPAQLMDNSTIPGTALGTEPCLGGHCLNSSLLVTGQPSSGRHPVLDLRGHKRKLATP SVSQESVRRRSRKGHLPAPCSPMNTGIQSLAGFQCHLSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134520
ORF Size	3897 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134520.1, NP_001127992.1
RefSeq Size	6629 bp
RefSeq ORF	3900 bp
Locus ID	363135
MW	144.8 kDa

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