Ppp3ca (NM_017041) Rat Tagged ORF Clone
CAT#: RR215113
- TrueORF®
Ppp3ca (Myc-DDK-tagged ORF) - Rat protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (Ppp3ca), (10 ug)
"NM_017041" in other vectors (3)
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CNY 5,816.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Calna1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR215113 representing NM_017041
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGAGCCCAAGGCGATTGATCCCAAGTTGTCGACTACGGACAGGGTGGTGAAAGCCGTTCCATTTC CGCCAAGTCACCGGCTGACAGCAAAGGAAGTGTTTGATAACGATGGGAAGCCTCGTGTGGATATCTTAAA AGCACATCTCATGAAGGAAGGCAGGCTGGAAGAAAGTGTCGCGTTGAGAATAATAACAGAGGGTGCTTCG ATTCTCCGACAGGAAAAAAACTTGCTGGATATTGATGCCCCAGTCACAGTTTGCGGGGACATCCATGGAC AATTCTTTGACTTGATGAAGCTCTTTGAAGTGGGAGGATCTCCTGCCAACACTCGCTACCTCTTCTTAGG GGACTATGTTGACAGAGGGTACTTCAGTATCGAATGTGTGCTGTATTTGTGGGCCTTGAAAATTCTTTAC CCCAAAACACTGTTTTTACTTCGTGGAAACCATGAATGTAGGCACCTAACAGAGTATTTCACGTTTAAAC AAGAATGTAAAATAAAGTATTCAGAACGCGTTTATGACGCCTGTATGGATGCCTTCGACTGCCTTCCCCT GGCTGCGCTGATGAACCAACAATTCCTGTGTGTACACGGTGGTTTGTCTCCAGAGATTAACACTCTAGAT GACATCAGAAAATTAGACCGATTCAAAGAACCACCTGCTTATGGGCCTATGTGTGACATCTTGTGGTCAG ACCCCCTGGAGGACTTTGGAAATGAGAAGACTCAGGAACATTTCACTCACAACACAGTCAGGGGTTGTTC GTACTTCTACAGTTACCCGGCTGTATGTGACTTCCTGCAGCACAATAATTTGTTGTCCATACTCCGAGCC CACGAAGCCCAGGACGCAGGGTACCGCATGTACAGGAAAAGCCAAACAACTGGCTTCCCGTCTCTAATTA CGATCTTCTCGGCACCAAATTACTTAGATGTGTACAATAATAAAGCTGCAGTGTTGAAGTACGAGAACAA CGTGATGAACATCAGGCAGTTCAACTGCTCCCCCCATCCGTACTGGCTCCCAAATTTCATGGATGTTTTC ACCTGGTCGCTGCCATTTGTTGGGGAGAAAGTGACTGAGATGCTGGTAAACGTCCTGAACATCTGCTCAG ATGATGAACTGGGGTCAGAAGAAGATGGATTTGACGGAGCCACGGCTGCAGCCCGGAAGGAGGTCATCAG GAACAAGATCCGAGCAATAGGCAAAATGGCCAGAGTATTCTCAGTTCTCAGAGAAGAGAGTGAGAGCGTT CTAACTCTGAAGGGCCTGACCCCGACTGGCATGCTCCCCAGCGGAGTGCTCTCTGGCGGGAAACAAACTC TGCAAAGCGCTACTGTTGAGGCCATTGAGGCTGATGAAGCCATCAAAGGATTCTCACCACAACATAAGAT TACCAGCTTCGAGGAGGCCAAGGGCTTAGACCGAATTAACGAGAGGATGCCGCCTCGCAGAGACGCCATG CCTTCCGACGCCAACCTTAACTCCATCAACAAGGCTCTCGCCTCAGAGACTAACGGCACAGACAGCAACG GCAGTAATAGCAGCAATATTCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR215113 representing NM_017041
Red=Cloning site Green=Tags(s) MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGAS ILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILY PKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLD DIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCDFLQHNNLLSILRA HEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVF TWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESV LTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAM PSDANLNSINKALASETNGTDSNGSNSSNIQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017041 |
ORF Size | 1563 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017041.1, NP_058737.1 |
RefSeq Size | 2337 bp |
RefSeq ORF | 1566 bp |
Locus ID | 24674 |
UniProt ID | P63329 |
MW | 58.6 kDa |
Gene Summary | catalytic subunit of Calcineurin (CaN), ubiquitously expressed Ca2+/CaM-dependent protein phosphatase; mediates activities of transcription factors and ion channels; involved in regulation of T-cell activation [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN215113 | Ppp3ca (untagged ORF) - Rat protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (Ppp3ca), (10 ug) |
CNY 6,650.00 |
|
RR215113L3 | Lenti ORF clone of Ppp3ca (Myc-DDK-tagged ORF) - Rat protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (Ppp3ca), (10 ug) |
CNY 8,650.00 |
|
RR215113L4 | Lenti ORF clone of Ppp3ca (mGFP-tagged ORF) - Rat protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (Ppp3ca), (10 ug) |
CNY 9,500.00 |