Nek1 (NM_001106082) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214766 representing NM_001106082 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGTATGTTAGACTACAGAAGATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCTA CAGAGGATGGCAGACATTATGTCATCAAGGAAATTAACATCTCAAGAATGTCCGATAAAGAAAGAGAAGA ATCAAGGAGAGAGGTCGCAGTGTTGGCAAACATGAAGCATCCAAATATTGTCCAATACAAAGAATCATTC GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATCTGTTTAAACGAATAAATG CTCAGAAAGGCACTTTGTTTCAAGAAGATCAGATTTTGGACTGGTTTGTTCAGATATGTTTGGCTTTGAA GCATGTACACGATAGAAAAATTCTTCACCGAGACATAAAGTCACAGAACATATTTCTAACCAAAGATGGG ACAGTGCAGCTTGGAGATTTTGGAATTGCTCGAGTTCTCAATAGTACTGTAGAGCTGGCTCGAACTTGCA TAGGGACTCCATACTACTTGTCACCTGAAATCTGTGAAAACAAGCCTTACAACAATAAAAGTGACATTTG GGCTTTGGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAAAAAC CTGGTACTGAAGATAATATCTGGATCCTTTCCTCCAGTGTCTCTACATTATTCCTATGATCTCCGCAGCT TGCTCTCTCAGCTGTTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGCTT TATAGCTAAACGAATCGAAAAGTTTCTCTCACCTCAGCTTATTGCAGAAGAATTTTGTCTGAAAACAGTT TCAAAGTTTGGACCACAGCCTATCCCAGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCC CTGCTCAGAAAATCACAAAGCCTTCTGCTAAATATGGAGTGCCTATAACATATAAGAAGTACGGAGATAA AAAGTTACTTGAAAAAAAACCACCCCCAAAGCATAAGCAGGCCCATCAGATTCCAGTGAAGAAAATGAAT TCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAACGAAGGTTGGAATTTATTGAGAAAG AAAAGAAGCAAAAGGATCAGATCAGGTTCCTGAAGGCTGAGCAGCTGAAGAGGCAAGAGAAGCAGCGGTT GGAGAGGATAAATAGGGCCAGGGAACAAGGATGGAGGAATGTTTTAAGGGCTGGTGGAAGCGGTGAAGTA AAGGCTTCCTTTTTTGGCAGTGGAGGAGCTGTGTCTCCATCACCTTGTTCTCCTCGGGGCCAGTATGAAC ATTACCATGCCATCTTTGACCAAATGCAGCAGCTAAGAGCAGAAGATAATGAAGCGAGATGGAAAGGGAG AATATATGGTCGATGGCTCCCTGAAAGGCAAAAAGGACACCTAGGTGTAGAGAGAGCCAAACAAGTGGAA GAATTCCTACAGCGTAAACAAGAAGCTATGCAGAATAAAGCCCGAGCAGAAGGACATGTGGTTTATCTGG CAAGACTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGA GAATAAAGAAGCTGGTGGTACCAAAGGACAAGACGCAACTGAAGAGGCTGACATGAGGCTCAAAAAGATG GAGTCACTTAAGGCCCAAGCAAATGCACGTGCTGCTGTACTAAAAGAACAGCTAGAGCGAAAAAGAAAGG AAGCTTATGAAAGAGAAAAGAAAGTGTGGGAAGAACATTTGGTGGTGAGGGTAAAAAGCTCAGATGTCCC TCCATCTATGGAGCATCATGAAGCAGGTGGTTCTCCATCACAGCAGCAGATGAAACCTGTTATTTCTGTG ACTTCAGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGAGTCCCAAGAAACTTCAGAAGAAATGG AAAAGAGTAACAATGCTATTTCAAGTAAGCGAGAAATACTGCGTAGGCTGAATGAAAATCTTAAAGCTCA AGAGGATGAAAAGGAAAAGCATCATCACGCTGATTCTTGTGAGACCGTTGGTCACAGAGATGAGAGAGAG TATGAGAAGGAAAATGCCATTTCCTCTGATCGCAAGAAATGGGAGATGGGAGGTCAGCTTGTGATTCCTC TGGATGAAGTGACACTGGATGCATCCTTCTCTGCTTCTGAAAAACATACTGTGGGAGAGGTTATTAAGTT AGATTCTAGTGGCTCTCCAAGAAAAGTCTGGGGGAAAAGCCCTACAGATTCTGTGCTGAAGATACTTGGA GAAGCTGAATTACAGCTACAGACAGAACTACTAGAAAACACGCCTTTTAAAAGTGAGGTTTGTGTTGAAG AGGAGAACTACAAACCCCTACTCACTGGAGAAGAGAATCTGCAGTGTATTTCAAAAGAAGTAAACCCATC AGCCACTGTTGATACTGAAACAAACAGTCCAAAGTTTGCTAAGGTGTCTCCAGCAATGTCCGAAGGAAAT GTGGAAGAACCTGATGATTTGGAAACAGAAGTTCTACAAGAGCCAAGTGCCATACCCTCAGATGGGAGTT TGCCGCCTGTTGTTAATGAGGTGTGGACCAGAGCTAAGGCAACTGGGTTGGAGGATAGCATTGCTGTGCA GCAGAATGACGTTTGTGAAGAGCGAATTCCAGGGACTGTGGACCAGTCCTGTAAGGATCATATAGAACCT GCAGGAAACGATTCTCCGCAGTCTGGCTGTGATGAAGAGAAGTCAGTACAGCCAGAATTGTTTTTCCAGA AAGTGGTTCACTCCAAGGACTTGAACTTAGTTCAGTCAGCTCATCGTTCCCCAGAAGAAGCAATTCCAAT TCGATCTCACTCTGATTCTCCACCAAAAGCTAAAAGCAAGAATTCCCTACTGATTGGACTTTCCACTGGT CTGTTTGATGCAAACAACCCAAAGATGCTGAGGACCTGTTCACTTCCAGACCTTTCCAAGCTCTTCAGAA CCCTAATGGATGTTCCCACTGTGGGAGACGTTCATCAAGACAGTCTTGAAATAGAGGAGCTTGAGGATGA ACATATTAAAGAAGGGCCCTCGGATTGCGAGGACATTGTATTTGAAGAAACTGACACAGATTTACAGGAG CTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCCGGTGACGAATACAGTGAGGAGGAAGAGTCTG TCTTAAAAAGCAGCAATGTGGAGCGGGCAGCAAGAGGGACAGATGTCCCAGATGAGGAGGACAACCCCAG CAGTGAAAGCGCCCTAAACGAGGAATGGCACTCAGATAACAGTGATGCTGAGACTACTAGTGAGTGTGAA TATGACAGTGTCTTTAACCATTTAGAGGAACTAAGACTTCACTTGGAACAAGAAATGGGCTTTGAAAAGT TCTTTGAGGTCTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGATGAAAATATTGAAATTTCTTCAAC CATAGTCGAGAACATCTTGGGCACTGAGCATCAGCACCTTTATGCCAAGATTCTGCATCTAGTCATGGCA GACGGAGCCTATCAGGAAGATAATGATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214766 representing NM_001106082 Red=Cloning site Green=Tags(s) MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKEREESRREVAVLANMKHPNIVQYKESF EENGSLYIVMDYCEGGDLFKRINAQKGTLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDG TVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKN LVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTV SKFGPQPIPGKRPASGQGVSSFVPAQKITKPSAKYGVPITYKKYGDKKLLEKKPPPKHKQAHQIPVKKMN SGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQLKRQEKQRLERINRAREQGWRNVLRAGGSGEV KASFFGSGGAVSPSPCSPRGQYEHYHAIFDQMQQLRAEDNEARWKGRIYGRWLPERQKGHLGVERAKQVE EFLQRKQEAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKEAGGTKGQDATEEADMRLKKM ESLKAQANARAAVLKEQLERKRKEAYEREKKVWEEHLVVRVKSSDVPPSMEHHEAGGSPSQQQMKPVISV TSALKEVGLDGSLTESQETSEEMEKSNNAISSKREILRRLNENLKAQEDEKEKHHHADSCETVGHRDERE YEKENAISSDRKKWEMGGQLVIPLDEVTLDASFSASEKHTVGEVIKLDSSGSPRKVWGKSPTDSVLKILG EAELQLQTELLENTPFKSEVCVEEENYKPLLTGEENLQCISKEVNPSATVDTETNSPKFAKVSPAMSEGN VEEPDDLETEVLQEPSAIPSDGSLPPVVNEVWTRAKATGLEDSIAVQQNDVCEERIPGTVDQSCKDHIEP AGNDSPQSGCDEEKSVQPELFFQKVVHSKDLNLVQSAHRSPEEAIPIRSHSDSPPKAKSKNSLLIGLSTG LFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIEELEDEHIKEGPSDCEDIVFEETDTDLQE LQASMEQLLREQPGDEYSEEEESVLKSSNVERAARGTDVPDEEDNPSSESALNEEWHSDNSDAETTSECE YDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEISSTIVENILGTEHQHLYAKILHLVMA DGAYQEDNDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106082
ORF Size	3600 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106082.2, NP_001099552.2
RefSeq Size	4895 bp
RefSeq ORF	3603 bp
Locus ID	290705
MW	136.1 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.