Atp2b1 (NM_053311) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Pmca1a; Pmca1b; Pmca1c
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214747 representing NM_053311 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACATGGCAAACAACTCAGTTGCATATAGTGGTGTGAAAAACTCTTTGAAAGAAGCTAATCATG ATGGCGACTTTGGCATCACACTTGCCGAGCTGCGGGCCCTCATGGAGCTCAGGTCCACAGATGCATTACG AAAAATACAGGAAAGCTATGGGGATGTCTATGGAATTTGCACCAAGTTGAAAACATCTCCCAATGAAGGT TTAAGTGGAAATCCTGCAGATTTAGAAAGAAGAGAAGCAGTGTTTGGAAAGAACTTTATACCTCCTAAGA AGCCAAAAACCTTTCTTCAGTTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATACTAGAAATCGC AGCCATAGTATCATTGGGCCTTTCTTTTTATCAACCTCCGGAAGGGGATAATGCACTTTGTGGAGAAGTT TCTGTTGGGGAAGAAGAAGGTGAAGGAGAAACGGGGTGGATTGAAGGAGCTGCCATCCTCTTGTCGGTGG TGTGTGTGGTGTTAGTGACGGCTTTCAATGACTGGAGTAAGGAGAAACAGTTCCGAGGCTTGCAGAGTCG AATTGAACAGGAACAGAAGTTCACCGTCATCAGGGGTGGGCAGGTCATCCAGATACCTGTAGCTGACATT ACTGTTGGAGACATTGCCCAAGTGAAGTACGGTGATCTTCTTCCAGCTGATGGTATATTGATTCAAGGCA ATGACCTTAAAATTGACGAGAGTTCGTTGACTGGCGAGTCTGACCATGTTAAGAAGTCTCTAGATAAAGA CCCCTTGCTTCTTTCAGGTACTCATGTAATGGAAGGTTCTGGAAGGATGGTCGTTACTGCTGTAGGTGTG AACTCTCAAACTGGGATCATCTTTACCTTACTTGGAGCTGGAGGCGAAGAGGAGGAAAAGAAAGATGAGA AGAAGAAAGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAAAATCGCAACAAAGCAAAAGCCCAAGA CGGCGCGGCCATGGAGATGCAGCCTCTGAAGAGTGAAGAAGGGGGAGATGGTGATGAGAAAGACAAGAAG AAAGCAAATCTGCCAAAGAAGGAAAAGTCTGTGTTACAAGGGAAACTTACGAAGCTGGCTGTTCAGATTG GCAAAGCAGGTCTGCTGATGTCTGCCATCACGGTTATCATCCTAGTGCTATATTTTGTGATCGATACCTT CTGGGTTCAGAAGAGGCCGTGGCTTGCTGAGTGCACGCCAATCTACATCCAGTACTTTGTGAAGTTCTTC ATTATCGGGGTCACCGTTCTAGTGGTCGCGGTGCCGGAAGGCCTCCCGCTGGCCGTCACGATCTCACTGG CTTACTCCGTCAAGAAAATGATGAAGGATAACAACTTGGTGAGGCACCTGGACGCTTGTGAGACCATGGG GAACGCTACAGCTATCTGTTCCGATAAGACGGGGACCCTGACGATGAACAGGATGACGGTCGTTCAGGCC TACATCAACGAAAAGCATTATAAAAAGGTTCCGGAACCAGAAGCCATCCCGCCAAATATCCTGTCCTATC TGGTAACAGGAATTTCTGTGAATTGTGCTTATACATCAAAAATATTGCCACCGGAGAAAGAGGGTGGATT ACCTCGTCATGTGGGGAATAAAACAGAATGTGCCTTGTTGGGATTTCTCTTGGATTTAAAGCGGGATTAT CAAGATGTTAGAAATGAAATACCAGAAGAAGCATTGTACAAAGTCTATACCTTCAATTCTGTTAGGAAAT CCATGAGTACAGTCCTAAAGAATTCAGATGGAAGTTTCCGGATCTTCAGCAAGGGTGCATCTGAGATAAT TCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAATGGTGAGGCCAAAGTGTTCCGACCCAGAGACCGAGAT GACATTGTGAAGACGGTGATTGAGCCCATGGCATCTGAAGGCCTGAGAACCATATGCCTAGCGTTCAGAG ACTTCCCCGCAGGAGAGCCAGAACCAGAGTGGGATAACGAGAACGATGTCGTCACCGGCCTTACTTGCAT CGCTGTTGTGGGGATTGAAGATCCTGTGAGGCCTGAGGTACCAGAGGCAATAAAGAAATGTCAGAGGGCT GGAATTACCGTGCGCATGGTCACTGGTGATAATATTAACACAGCTCGGGCCATTGCTACCAAATGCGGTA TTTTACACCCAGGGGAAGATTTCCTGTGCTTAGAAGGTAAAGATTTTAACCGAAGAATACGAAATGAAAA AGGAGAGATTGAACAAGAGAGGATAGACAAGATTTGGCCAAAGCTTCGAGTGCTTGCAAGATCATCTCCC ACTGACAAACATACACTGGTTAAAGGTATAATTGACAGCACAGTCTCAGAGCAACGCCAGGTTGTAGCTG TGACAGGGGATGGTACAAATGACGGGCCTGCGCTGAAGAAAGCAGACGTTGGCTTTGCAATGGGCATTGC TGGAACTGATGTGGCTAAAGAAGCATCTGATATCATTCTCACAGATGACAACTTCACAAGCATTGTTAAA GCGGTTATGTGGGGACGGAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTCCAGCTTACTGTTAATG TAGTGGCCGTGATTGTCGCTTTCACGGGCGCTTGTATTACTCAAGACTCGCCACTGAAGGCGGTACAGAT GCTCTGGGTCAACCTCATCATGGACACGCTGGCCTCCCTGGCTCTGGCGACAGAACCACCCACCGAGTCA CTCCTGCTCCGGAAGCCTTACGGGAGAAACAAGCCTCTCATCTCACGCACAATGATGAAGAATATCTTGG GCCATGCGTTCTATCAGCTCGTAGTGGTCTTCACGCTCTTGTTCGCTGGAGAGAAGTTTTTCGATATCGA CAGTGGGAGGAACGCCCCTCTGCATGCTCCACCGTCAGAGCACTACACCATTGTGTTCAACACCTTTGTG CTGATGCAGCTCTTCAATGAAATAAATGCCCGGAAAATTCATGGGGAAAGAAATGTGTTTGAAGGGATCT TCAACAACGCCATCTTCTGCACAATTGTCTTAGGCACCTTTGTGGTGCAGATAATAATCGTGCAGTTTGG CGGGAAGCCTTTCAGCTGCTCAGAACTTTCAATAGAACAATGGCTGTGGTCCATATTCCTGGGAATGGGG ACCTTACTCTGGGGCCAGCTTATTTCAACCATTCCAACCAGCCGCTTAAAGTTTCTAAAAGAAGCTGGCC ATGGGACACAGAAGGAAGAGATCCCTGAGGAGGAATTGGCGGAGGATGTTGAAGAGATTGACCATGCCGA ACGGGAGTTGCGGCGTGGCCAGATCTTGTGGTTTAGAGGCCTGAACAGAATCCAAACACAGATGGATGTA GTGAATGCTTTCCAGAGTGGAGGTTCCATTCAGGGGGCTCTAAGGCGACAACCCTCCATCGCCAGCCAGC ACCATGATGTAACAAATGTTTCTACCCCTACACATGTAGTGTTTTCCTCTTCTACTGCTTCTACTCCTGT GGGGTATCCGAGTGGTGAATGCATTTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214747 representing NM_053311 Red=Cloning site Green=Tags(s) MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEG LSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEV SVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADI TVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGV NSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKK KANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFF IIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQA YINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGFLLDLKRDY QDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSFRIFSKGASEIILKKCFKILSANGEAKVFRPRDRD DIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDVVTGLTCIAVVGIEDPVRPEVPEAIKKCQRA GITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSP TDKHTLVKGIIDSTVSEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVK AVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTES LLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFV LMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMG TLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQMDV VNAFQSGGSIQGALRRQPSIASQHHDVTNVSTPTHVVFSSSTASTPVGYPSGECIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053311
ORF Size	3528 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053311.1, NP_445763.1
RefSeq Size	4228 bp
RefSeq ORF	3531 bp
Locus ID	29598
UniProt ID	P11505
MW	129.5 kDa
Gene Summary	regulates intracellular Ca2+ levels [RGD, Feb 2006]

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