Atp2b1 (NM_053311) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pmca1a; Pmca1b; Pmca1c
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214747 representing NM_053311 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACATGGCAAACAACTCAGTTGCATATAGTGGTGTGAAAAACTCTTTGAAAGAAGCTAATCATG ATGGCGACTTTGGCATCACACTTGCCGAGCTGCGGGCCCTCATGGAGCTCAGGTCCACAGATGCATTACG AAAAATACAGGAAAGCTATGGGGATGTCTATGGAATTTGCACCAAGTTGAAAACATCTCCCAATGAAGGT TTAAGTGGAAATCCTGCAGATTTAGAAAGAAGAGAAGCAGTGTTTGGAAAGAACTTTATACCTCCTAAGA AGCCAAAAACCTTTCTTCAGTTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATACTAGAAATCGC AGCCATAGTATCATTGGGCCTTTCTTTTTATCAACCTCCGGAAGGGGATAATGCACTTTGTGGAGAAGTT TCTGTTGGGGAAGAAGAAGGTGAAGGAGAAACGGGGTGGATTGAAGGAGCTGCCATCCTCTTGTCGGTGG TGTGTGTGGTGTTAGTGACGGCTTTCAATGACTGGAGTAAGGAGAAACAGTTCCGAGGCTTGCAGAGTCG AATTGAACAGGAACAGAAGTTCACCGTCATCAGGGGTGGGCAGGTCATCCAGATACCTGTAGCTGACATT ACTGTTGGAGACATTGCCCAAGTGAAGTACGGTGATCTTCTTCCAGCTGATGGTATATTGATTCAAGGCA ATGACCTTAAAATTGACGAGAGTTCGTTGACTGGCGAGTCTGACCATGTTAAGAAGTCTCTAGATAAAGA CCCCTTGCTTCTTTCAGGTACTCATGTAATGGAAGGTTCTGGAAGGATGGTCGTTACTGCTGTAGGTGTG AACTCTCAAACTGGGATCATCTTTACCTTACTTGGAGCTGGAGGCGAAGAGGAGGAAAAGAAAGATGAGA AGAAGAAAGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAAAATCGCAACAAAGCAAAAGCCCAAGA CGGCGCGGCCATGGAGATGCAGCCTCTGAAGAGTGAAGAAGGGGGAGATGGTGATGAGAAAGACAAGAAG AAAGCAAATCTGCCAAAGAAGGAAAAGTCTGTGTTACAAGGGAAACTTACGAAGCTGGCTGTTCAGATTG GCAAAGCAGGTCTGCTGATGTCTGCCATCACGGTTATCATCCTAGTGCTATATTTTGTGATCGATACCTT CTGGGTTCAGAAGAGGCCGTGGCTTGCTGAGTGCACGCCAATCTACATCCAGTACTTTGTGAAGTTCTTC ATTATCGGGGTCACCGTTCTAGTGGTCGCGGTGCCGGAAGGCCTCCCGCTGGCCGTCACGATCTCACTGG CTTACTCCGTCAAGAAAATGATGAAGGATAACAACTTGGTGAGGCACCTGGACGCTTGTGAGACCATGGG GAACGCTACAGCTATCTGTTCCGATAAGACGGGGACCCTGACGATGAACAGGATGACGGTCGTTCAGGCC TACATCAACGAAAAGCATTATAAAAAGGTTCCGGAACCAGAAGCCATCCCGCCAAATATCCTGTCCTATC TGGTAACAGGAATTTCTGTGAATTGTGCTTATACATCAAAAATATTGCCACCGGAGAAAGAGGGTGGATT ACCTCGTCATGTGGGGAATAAAACAGAATGTGCCTTGTTGGGATTTCTCTTGGATTTAAAGCGGGATTAT CAAGATGTTAGAAATGAAATACCAGAAGAAGCATTGTACAAAGTCTATACCTTCAATTCTGTTAGGAAAT CCATGAGTACAGTCCTAAAGAATTCAGATGGAAGTTTCCGGATCTTCAGCAAGGGTGCATCTGAGATAAT TCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAATGGTGAGGCCAAAGTGTTCCGACCCAGAGACCGAGAT GACATTGTGAAGACGGTGATTGAGCCCATGGCATCTGAAGGCCTGAGAACCATATGCCTAGCGTTCAGAG ACTTCCCCGCAGGAGAGCCAGAACCAGAGTGGGATAACGAGAACGATGTCGTCACCGGCCTTACTTGCAT CGCTGTTGTGGGGATTGAAGATCCTGTGAGGCCTGAGGTACCAGAGGCAATAAAGAAATGTCAGAGGGCT GGAATTACCGTGCGCATGGTCACTGGTGATAATATTAACACAGCTCGGGCCATTGCTACCAAATGCGGTA TTTTACACCCAGGGGAAGATTTCCTGTGCTTAGAAGGTAAAGATTTTAACCGAAGAATACGAAATGAAAA AGGAGAGATTGAACAAGAGAGGATAGACAAGATTTGGCCAAAGCTTCGAGTGCTTGCAAGATCATCTCCC ACTGACAAACATACACTGGTTAAAGGTATAATTGACAGCACAGTCTCAGAGCAACGCCAGGTTGTAGCTG TGACAGGGGATGGTACAAATGACGGGCCTGCGCTGAAGAAAGCAGACGTTGGCTTTGCAATGGGCATTGC TGGAACTGATGTGGCTAAAGAAGCATCTGATATCATTCTCACAGATGACAACTTCACAAGCATTGTTAAA GCGGTTATGTGGGGACGGAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTCCAGCTTACTGTTAATG TAGTGGCCGTGATTGTCGCTTTCACGGGCGCTTGTATTACTCAAGACTCGCCACTGAAGGCGGTACAGAT GCTCTGGGTCAACCTCATCATGGACACGCTGGCCTCCCTGGCTCTGGCGACAGAACCACCCACCGAGTCA CTCCTGCTCCGGAAGCCTTACGGGAGAAACAAGCCTCTCATCTCACGCACAATGATGAAGAATATCTTGG GCCATGCGTTCTATCAGCTCGTAGTGGTCTTCACGCTCTTGTTCGCTGGAGAGAAGTTTTTCGATATCGA CAGTGGGAGGAACGCCCCTCTGCATGCTCCACCGTCAGAGCACTACACCATTGTGTTCAACACCTTTGTG CTGATGCAGCTCTTCAATGAAATAAATGCCCGGAAAATTCATGGGGAAAGAAATGTGTTTGAAGGGATCT TCAACAACGCCATCTTCTGCACAATTGTCTTAGGCACCTTTGTGGTGCAGATAATAATCGTGCAGTTTGG CGGGAAGCCTTTCAGCTGCTCAGAACTTTCAATAGAACAATGGCTGTGGTCCATATTCCTGGGAATGGGG ACCTTACTCTGGGGCCAGCTTATTTCAACCATTCCAACCAGCCGCTTAAAGTTTCTAAAAGAAGCTGGCC ATGGGACACAGAAGGAAGAGATCCCTGAGGAGGAATTGGCGGAGGATGTTGAAGAGATTGACCATGCCGA ACGGGAGTTGCGGCGTGGCCAGATCTTGTGGTTTAGAGGCCTGAACAGAATCCAAACACAGATGGATGTA GTGAATGCTTTCCAGAGTGGAGGTTCCATTCAGGGGGCTCTAAGGCGACAACCCTCCATCGCCAGCCAGC ACCATGATGTAACAAATGTTTCTACCCCTACACATGTAGTGTTTTCCTCTTCTACTGCTTCTACTCCTGT GGGGTATCCGAGTGGTGAATGCATTTCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053311
Insert Size	3531 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053311.1, NP_445763.1
RefSeq Size	4228 bp
RefSeq ORF	3531 bp
Locus ID	29598
UniProt ID	P11505
Gene Summary	regulates intracellular Ca2+ levels [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.