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Sema3e (NM_001106579) Rat Tagged ORF Clone
CAT＃: RR214586
- TrueORF®
Sema3e (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e), (10 ug)
"NM_001106579" in other vectors (3)
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CNY 9,980.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214586 representing NM_001106579
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCGACCGGACACATCCTCACCTTGCTGCTCTGGGGTCACCTGCTGGAGCTCTGGACCCCAGGTT ACACTGCGAACCCCTCCTATCCCAGGCTACGACTGTCTCATAAAGAACTCTTGGAACTGAACAGGACGTC AATATTTCAAAGCCCCTTTGGTTTTCTTGATCTCCACACAATGCTGCTGGATGAGTATCAAGAACGGCTC TTTGTGGGAGGCAGAGACCTTGTCTATTCCCTGAACTTGGAACGCGTCAGTGATGGCTACAGAGAGATAT ACTGGCCTAGCACAGCAGTAAAAGTAGAAGAATGCATAATGAAAGGAAAAGACGCAAATGAGTGTGCCAA TTATGTCCGGGTTTTGCATCACTACAACAGGACACACCTTCTGACCTGTGCTACGGGAGCCTTTGATCCA CACTGTGCCTTTATCCGAGTCGGGCACCATTCAGAGGACCCCCTGTTTCACCTGGAGTCACACAGATCTG AGAGAGGAAGGGGCAGATGTCCTTTTGACCCCAACTCCTCCTTTGTGTCCACACTAGTTGGTAATGAACT GTTTGCTGGGCTCTACAGTGACTACTGGGGCCGAGACTCAGCGATCTTCCGCAGCCTGGGGAAGCTCGGC CATATCCGCACCGAGCATGACGATGAGCGGCTGCTGAAAGAACCAAAATTTGTAGGTTCATATATGATTC CTGATAACGAAGACCCAGAGGACAACAAAATGTACTTCTTCTTCACTGAGAAGGCATTGGAGGCAGAGAA CAATGCCCACACGATCTACACCCGAGTGGGGCGGCTGTGCGTGAATGACATGGGAGGACAGAGAATCCTG GTGAATAAGTGGAGCACATTCCTCAAAGCACGGCTGGTTTGCTCAGTACCAGGGATGAATGGAATTGACA CACACTTTGACGAATTAGAGGATGTGTTTTTACTGCCTACCCGAGATCCCAAGAATCCAGTGATATTTGG ACTATTTAATACTACCAGCAATATTTTTAGAGGCCATGCTGTATGTGTGTATCACATGTCAAGTATCCGG GAAGCCTTTAATGGCCCATATGCACATAAAGAAGGCCCTGAATATCACTGGTCACTGTATGAAGGAAAGG TCCCCTACCCAAGGCCTGGTTCTTGTGCCAGCAAAGTGAATGGAGGAAAGTACGGATCCACCAAAGACTA CCCTGACGACGCCATCCGATTCGCAAGGATGCATCCACTAATGTATCAGCCCATAAAACCTGTCCATAAA AAACCAATACTGGTAAAAACGGATGGAAAATATAACCTGAGGCAACTAGCAGTGGACCGAGTGGAAGCGG AGGATGGCCAGTATGACGTTTTATTTATTGGAACAGACACAGGAATCGTGCTGAAAGTAATCACAATTTA CAACCAAGAAACAGAGTGGATGGAGGAAGTCATTCTGGAGGAACTTCAAATATTCAAGGATCCAGCCCCT ATCATTTCTATGGAGATTTCTTCAAAGAGGCAACAGCTCTATATTGGGTCAGCCTCTGCTGTGGCACAAG TCAGATTCCATCACTGTGACATGTACGGCAGCGCCTGTGCTGACTGCTGCCTGGCTCGAGACCCTTACTG TGCCTGGGATGGTATCTCCTGCTCCAGGTACTACCCGACAGGTGCACACTCAAAGAGGAGATTCCGCAGG CAGGATGTTCGGCACGGCAACGCCGCCCAGCAGTGCTTTGGGCAGCAATTTGTGGGAGACGCTTTGGATA GGACTGAAGAGAGGCTGGCTTATGGCATAGAGAGCAACAGTACTTTGCTGGAATGCACCCCACGATCACT ACAAGCAAAAGTTGTCTGGTTTGTACAGAAGGGACGCGACGTAAGAAAGGAAGAGGTAAAGACGGATGAC AGAGTTGTTAAGATGGACTTGGGCTTGCTCTTCCTCAGAGTCCGCAAGTCGGATGCAGGGACCTACTTTT GCCAAACGGTAGAACATAGCTTCGTCCACACTGTGCGCAAAATCACCTTGGAGGTGGTCGAAGAGCATAA AGTGGAGGGGATGTTTCATAAGGACCGCGAAGAGGAAAAACATCACAAGATGCCCTGCCCTCCCTTAACT GGTATGTCTCAGGGGACAAAACCCTGGTACAAGGAATTCTTGCAGCTGATTGGCTACAGCAACTTCCAGA GAGTGGAAGAATACTGCGAAAAGGTGTGGTGTACAGATAAGAAGAGGAAAAAGCTGAAAATGTCTCCCTC CAAGTGGAAGTATGCCAACCCCCAGGAAAAGAGGCTTCGCTCCAAAGCTGAGCATTTCCGCCTGCCCAGG CACACGCTGGTCTCT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR214586 representing NM_001106579
Red=Cloning site Green=Tags(s) MAPTGHILTLLLWGHLLELWTPGYTANPSYPRLRLSHKELLELNRTSIFQSPFGFLDLHTMLLDEYQERL FVGGRDLVYSLNLERVSDGYREIYWPSTAVKVEECIMKGKDANECANYVRVLHHYNRTHLLTCATGAFDP HCAFIRVGHHSEDPLFHLESHRSERGRGRCPFDPNSSFVSTLVGNELFAGLYSDYWGRDSAIFRSLGKLG HIRTEHDDERLLKEPKFVGSYMIPDNEDPEDNKMYFFFTEKALEAENNAHTIYTRVGRLCVNDMGGQRIL VNKWSTFLKARLVCSVPGMNGIDTHFDELEDVFLLPTRDPKNPVIFGLFNTTSNIFRGHAVCVYHMSSIR EAFNGPYAHKEGPEYHWSLYEGKVPYPRPGSCASKVNGGKYGSTKDYPDDAIRFARMHPLMYQPIKPVHK KPILVKTDGKYNLRQLAVDRVEAEDGQYDVLFIGTDTGIVLKVITIYNQETEWMEEVILEELQIFKDPAP IISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGAHSKRRFRR QDVRHGNAAQQCFGQQFVGDALDRTEERLAYGIESNSTLLECTPRSLQAKVVWFVQKGRDVRKEEVKTDD RVVKMDLGLLFLRVRKSDAGTYFCQTVEHSFVHTVRKITLEVVEEHKVEGMFHKDREEEKHHKMPCPPLT GMSQGTKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDKKRKKLKMSPSKWKYANPQEKRLRSKAEHFRLPR HTLVS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106579 |
| ORF Size | 2325 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106579.1, NP_001100049.1 |
| RefSeq Size | 4476 bp |
| RefSeq ORF | 2328 bp |
| Locus ID | 296789 |
| MW | 89.5 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214586 | Sema3e (untagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e), (10 ug) |
CNY 9,980.00 |
|
| RR214586L3 | Lenti ORF clone of Sema3e (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e), (10 ug) |
CNY 11,780.00 |
|
| RR214586L4 | Lenti ORF clone of Sema3e (mGFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e), (10 ug) |
CNY 12,920.00 |
