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Uvssa (NM_001134558) Rat Tagged ORF Clone
CAT＃: RR214497
- TrueORF®
Uvssa (Myc-DDK-tagged ORF) - Rat similar to putative nuclear protein, with 2 coiled coil-4 domains, of eukaryotic origin (5I282) (RGD1306371), (10 ug)
"NM_001134558" in other vectors (3)
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CNY 9,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1306371 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214497 representing NM_001134558
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCAGAAACTTTCACAGTTGATAGAGGAGCTCACAACTTCAGGAGAATCCCAACTGAATGCTCAGA AAATGAAGGAACTGAAGAAAATTTGCAAGTCTTCAGAGGAGCAGCTGAGCCATGCCTACCGCCTGCTAAT GACACAGCTGACCCAGGACCACGCTGAGATCCGCCTCTCAGCCTTCCAGATCGTGGATGAGCTCTTCACC CGGTCCCATCAGTTCAGAGTGCTGCTTGTTTCTGACTTCCAGGAATTCCTGGAGCTCACACTGGGCACAG ACAATGACCATCCCTTGCCACCCCCTCGGGAGGCAGCTCAGAGGCTAAGGCAGGCAGCCATGCAAGCTGT GGAAGGTTGGAATGAGAAGTTTGGGGAAGCCTATAAGAAGCTAGCCTTGGGCTACCACTTCCTAAAACAC ACCAAAAAGGTGGATTTTCGGGATATAAATGTTAGGACTCTGGCAGAAAGGAAGCGAGAAGAGGAGAAGC AGAAGCACTTGGATAAGATCCACAGAGAAAGTGCTGACCGGGCTAAGAGGGAGATGGAAGAAATGTCTGA TGAAATTGGATGCTGCCTGACAGAAGTGGAGAACTGCTTTAGGCTGCTGGTGCCCTTGGATTTGGGCCCG TACCGAGAGGATAAGTTCTTTGGTGAGGCATCTGGCATAGCAGAGGACCATGCTCCTTGCGCCTGGAGCC CAGACCTGGCAACTCCCCGAGGGTCTGGTCTCTCTGGACCCCAGGATGAAGAGCAGCCATGCTGTAGCAA GGACCTGGTTGCCTCTGCACACCATGCAGGATCTGCAGTTGGTCTGAAGGCACCAGCCCCAGCAGCCACG GAAGACCCCTGCAGGGATGAAGACAGACACAGCGAACACAGCGACCCAGAGGATTTCCTGCGGAGCCATG GGCTGGGCTCCCACAAGTACACGCTGGACGTGGAACTCCCCTCAGACGGTCTGAAGGTACAGGAGAATGA AGACAACCTGGCCGTGCTCCACGCTGCTCGAGACTCGCTCAAACTCATCCAGAACAAGTTTCTGCCAGCG GTGTGCTCCTGGGTCCAGCGGTTTACCCGTGCAGGGATCTACAGTGGACATTTAAAGCAGGCCATTGACC TGAAAATGGAACTGGAACTTGCTCTGAAGAAATATGAAGAACTGAACATTGAGCCTGGGAGAGGACAAAG GAGCAGGACAGAAGCACTGGAGGACAGTGAGGAGGAGGACCAGGACTTCGTGGAGGTTCCAGAGAAAGAG GGTTACGAGCCTCGAATCCCCGACCATCTACGAGCTGAATATGGGCTGGAGCCAAAGGCCCCACTGAAGA CTCTGGAGAAACATACAGCTGTATGTAGCGTACAGGAGAGGACCAGGAGGAGAAGGGAAGAGGAGGCCTC AGACCCCACCTCTGCAGCTGCCCAGATGTTGCGGCTCCAGGACTGCTTGTCATCTCCCTCCTCCTCCTCT ACCAGGGGACCTCTGGGACCAGAAGAAGCCCAGAAGCAAGCGGAGCGAGCCCGAGCACCCATGGTGCCCT TCGGAGTGGACCTGTGCTACTGGGGCCAGGAGCAGCTAACAGCTGGGAAGATTCTTAAATCCGACTCTCA ACACCGCTTCTGGAAACCCCACGAGGTAGAGGAAGAGGTGGACAGTGCCCATGTTTCTGAGATGCTCCAC AGCCGACACATTACCTTTTCTGGGAAATTCGAGCCTGTGCAGCACAAGTGCCGGGCCCTCAGGCCTAATG GCAGGCTCTGCGAGCGCCAGGATCGGCTGAAGTGCCCGTTCCATGGGAAGATCATCCCCAGAGATGACAA GGGGCAGCCTCTCAACCCTGAAGACAGAGCCCGTGAACAGAGGCAGCAGCTTCAGCAGCAACGGGCACAT CCAGATTGGCAGGACCCTGAGTTTATGAAGGACGTGGAGGCAGCCACAGGGGTGGACCTCGGTTCCTCCA AGTATAGCAAGAAGGGCAAAGGGAAAAAGAAGAAGCACCCCAACCTCACTGACCTTCGAGAACGTGCCAA CACTGCCCGAGCCCGCCTTGAGAAGAAGGTCTTTGCCAAGAGCTGTGCAGAGAGTAGTTGCTGCCATGAA CCAGATGGACCAGAAGAAGCACGAGAAGTTTGCAAATCAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214497 representing NM_001134558
Red=Cloning site Green=Tags(s) MDQKLSQLIEELTTSGESQLNAQKMKELKKICKSSEEQLSHAYRLLMTQLTQDHAEIRLSAFQIVDELFT RSHQFRVLLVSDFQEFLELTLGTDNDHPLPPPREAAQRLRQAAMQAVEGWNEKFGEAYKKLALGYHFLKH TKKVDFRDINVRTLAERKREEEKQKHLDKIHRESADRAKREMEEMSDEIGCCLTEVENCFRLLVPLDLGP YREDKFFGEASGIAEDHAPCAWSPDLATPRGSGLSGPQDEEQPCCSKDLVASAHHAGSAVGLKAPAPAAT EDPCRDEDRHSEHSDPEDFLRSHGLGSHKYTLDVELPSDGLKVQENEDNLAVLHAARDSLKLIQNKFLPA VCSWVQRFTRAGIYSGHLKQAIDLKMELELALKKYEELNIEPGRGQRSRTEALEDSEEEDQDFVEVPEKE GYEPRIPDHLRAEYGLEPKAPLKTLEKHTAVCSVQERTRRRREEEASDPTSAAAQMLRLQDCLSSPSSSS TRGPLGPEEAQKQAERARAPMVPFGVDLCYWGQEQLTAGKILKSDSQHRFWKPHEVEEEVDSAHVSEMLH SRHITFSGKFEPVQHKCRALRPNGRLCERQDRLKCPFHGKIIPRDDKGQPLNPEDRAREQRQQLQQQRAH PDWQDPEFMKDVEAATGVDLGSSKYSKKGKGKKKKHPNLTDLRERANTARARLEKKVFAKSCAESSCCHE PDGPEEAREVCKSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001134558 |
| ORF Size | 2142 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001134558.1, NP_001128030.1 |
| RefSeq Size | 2632 bp |
| RefSeq ORF | 2145 bp |
| Locus ID | 314061 |
| UniProt ID | D3ZND0 |
| MW | 81.1 kDa |
| Gene Summary | Factor involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage. TC-NER allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Acts by promoting stabilization of ERCC6 by recruiting deubiquitinating enzyme USP7 to TC-NER complexes, preventing UV-induced degradation of ERCC6 by the proteasome. Interacts with the elongating form of RNA polymerase II (RNA pol IIo) and facilitates its ubiquitination at UV damage sites, leading to promote RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery. Not involved in processing oxidative damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214497 | Uvssa (untagged ORF) - Rat similar to putative nuclear protein, with 2 coiled coil-4 domains, of eukaryotic origin (5I282) (RGD1306371), (10 ug) |
CNY 9,120.00 |
|
| RR214497L3 | Lenti ORF clone of Uvssa (Myc-DDK-tagged ORF) - Rat similar to putative nuclear protein, with 2 coiled coil-4 domains, of eukaryotic origin (5I282) (RGD1306371), (10 ug) |
CNY 10,930.00 |
|
| RR214497L4 | Lenti ORF clone of Uvssa (mGFP-tagged ORF) - Rat similar to putative nuclear protein, with 2 coiled coil-4 domains, of eukaryotic origin (5I282) (RGD1306371), (10 ug) |
CNY 12,070.00 |
