Ttc3 (NM_001108315) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214244 representing NM_001108315 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGGCATCGGGGCGCTCCACACCTCCCTCCGTGGGTGTGTGGACTGTGCAGCATGGATGACTTTG CTGAGGGAGGTCTCAGTTTGGCAGATGATATCTTACTGGAAGATTACCCTTATGAGGATGACTGTATCTG TACTCCTGACTTTACCACTGATGATTATGTTCGAGTAACCCAGCTTTACTATGAAGGCGTGGGTATGCAA TATAAAGATTATGCCCAAAGTGAGAAAAATTTAGAGTATGACATCTGCAATATATGGTGCAGTAAGCCAC TGTCCATCCTGCAAGATTACTGTGATGCCATTAAGCTGTACATCTTCTGGCCACTTCTCTTTCAACATCA GCACAGTTCTATAATATCAAGATTGCACCCCTGTGTAGAAGCCATCCGTTCTCGTGCTGCTGAGATAAGT TTGAAGAAATTACAACATCTTGAGTTGATGGAAGACATTGTGGATTTGGCAAAGAAAGTTGCAAATGATT CATTCCTTATTGAAGGCTTATTGAAAATTGGTTATAAAATAGAAAATAAAATCTTGGCAATGGAAGATGC TTTAAATTGGATAAAATACACGGGTGATGTAACAATTCTACCTAAATTAGGATCAGTTGACAGTGGCTGG CCCATGTTAAGTATTTTCTTTACTGAATATAAGTACCATATTACTAGAGTTGTAACTGAAAATTGCAACT TGCTAGAAGAATTTAGAAGGCATAGTTGCATGCAGTGTGTGAAGCAAGGAGAACTCATGAAAATGAGAGG AAATGAAGAGTTTGCAAAGGAAAAGTTTGAAATAGCTGTTATTTATTACACCAGAGCCATTGAATATAGA CCTGAAAACCATCTTCTTTATGGTAACCGAGCTCTCTGTTTTCTTCGTATGGGGCAGTTTAGAAATGCAC TTAGTGATGGAAAGAGGGCCATTGTTTTGAAGAACACCTGGCCGAAGGGTCATTATCGTTATTGTGATGC TCTTTCTATGCTGGGGGAATATGACTGGGCCCTGCAAGCAAACATAAAAGCTCAAAAACTCTGTAAAAAT GACCCTGAGGGAATCAAGGATCTAATTCAGCAGCATATAAAGTTACAAAAACAAATAGAAGACCTACAAG GTCGGACATCAAATAGGAATCCAATTAAAGCTTTTTATGAAAGCAGGGCTTACATACCTAGAAACTCATC AGCACCTGCTTTTAGAACATCACTTAACTTTGTGGAAACAGAAAGAGGTTTCAGGAAAACTAAGTACAGA ATGGCAAATGGCGGTGGCCATCAGAATCAAAAGGTGGCAGATGAGGCTTTGAAGGGGGACGATTGTGACT GTCATCCTGAATTTCTGCCACCTCCAAGTCAGCCTCCAAGACATAAAGGAAAACAAAAGTCACGGAACAA TGAATCAGAAAAGCCCAGTTCTAATTCTCAAGTGACTTTACAAGTAGATTTGAAAAGCATTTTGGAGAAA CAATTTTCAAAATCTTCCAGAGCTGCACACCAGGATTTTGCTAACATCATGAAAATGTTAAGAAGCTTGA TTCAGGATGGGTACACGGCCTTGTTGGAGCAGCGCTGCCGCAGTGCTGCCCAGGCCTTTACAGAGTTACT CAATGGCTTAGATCCTCAGAAAATAAAGCAATTGAACCTGGCCATGATTAATTATGTATTAGTAGTCTAT GGACTTGCTGTTTCTCTCCTTGGAATAGGACGACCTGAGGAACTGTCTGAAGCCGAAAACCAGTTTAAGA GGATCATCGAACACTATCCCAATGAGGGGCTTGACTGCTTGGCCTACTGTGGAATAGGAAAAGTATATTT GAAAAAAAACAGATTTCTCGAAGCTCTTAATCACTTTGAAAAAGCAAAAACTTTAATTTGTCGTCTCCCT GGAATTCTAACTTGGCCCACAAGTAATGTGATTATTGAAGAATCTAAGCCAGAAAAGGTCAAGGTGATGT TAGAGAAGTTTGTTGAAGAATGCAAGTTTCCTCCTGTGCCGGATGCTGTTTGTTGCTATCAGAAGTGCCG TGGGTTTTCCAAAATCCAGATTTACCTAACTGACCCAGACTTCAAGGGTTTTATACGAATTAGCTGTTGC CAATACTGTAAAGTAGAATTTCACATGAACTGCTGGAAAAAGTTAAAAACAACAACTTTTAATGATAAAA TTGACAAGGATTTTCTACAAGGAATTTGTCTCACCCCTGACTGTGAAGGAATCATTTCTAAGATTATCAT CTTTAGCAGTGGTGGCCAAGTTAAATGTGAATTTGAACACAAGGTCGTAAAAGAAAAGGCTCCTTCAAGA CCTGTTCTGAAACAGAAATGTTCTAGTCTAGAGAAGTTAAGACTGAAAGAAGACAAAAAATTGAAGAGAA AGATCCAAAAACAAGAGGCAAAAAAGTTGGCACAAGAAAGAATGGAAGAGGACTTAAGAGAAAGTAATCC ACCTAAACCTGAAGAGCCGGAAGAAACTGTAGAAAGCGCTCAGAGTTGTCAGTTTCTTGATGACAGAATC CTTCAGTGCATAAAGCAGAATGCTGACAAGATTAAATCTGTTGTGTTGAACACTTCCACGCTTCTCAAAG AGTTGCTGTCTTGGAAGGTTCTGAGCACAGAAGACTACACAACATGTTTTTCTAGCAAAAATTTTGTTCA TGAAGCAGTGGACTATGTTATCGGTCACTTGATTCAAGAAAAGAACAGAGTAAAGACACGGATATTTCTG CATGTTTTGAGTGAGCTTAAAGAGTTAGACCCCAAGTTAGCTCCCTGGATCCAGAGACTGAATAGCTTTG GCTTAGATGCCACAGGACCCTTTTTTACTCGCTATGGAGCATCTCTCAAAGAGCTTGATTTTAACATTGT GACTTTCCTTTGGAGTGAGAAATATGGCCATAAACTAGGTTCTATAGAAGGAAAGCAACTTGATTACTTC TGTGAGCCAGCATCAGTAATGGAAGCACGCTGCTTAATATGGCTGCTGGAAGAGCACAGGGACAAGTTCC CCGCCTTGCACAGTGCTTTAGACGAGTTCTTTGATATAATGGACAGCCGATGCACTGTGCTAAGGAAGCA AGACAGTGATGAAATGCCGTTTGGTTGTATCAAGGTAAAAAACAAAGGCAAGAAAAAGAAGCCAAAAGAT TCAAAGCCGATGTTAGTGGGATCTGGAACAGCATCAGTAACACCAAGTAGTGAGACTGTCACTCCAGAAG ACCATAACAGGCGCAATTCAGATTCTGCAGGACCATTTGCAGTGCCTGACCACCTTCGGCAAGATGTGGA AGAGTTCGAAGCTCTTTACGACCAGCACAGCAGCGAGTATGTTGTCCGAAATAAGAAGCTGTGGGATATT AACCCCAAACAGAAATGTTCAACTCTGTATGATTACTTCTCTCAGTTGCTGGAAGAGCACGGCCCCTTGG ATATGAGTGATAGGATGTTCTCTGAGGAATATGAGTTCTTCCCTGAAGAAACTCGCCAGATACTGGAGAA GGCAGGAGGGCTGAAGTCGTTCCTCCTGGGCTGTCCTCGATTTGTTGTGATTGACAACTGCATTGCATTG AAAAAAGTTGCCTCAAGACTCAAGAAAAAAAGAAAGAAGAAAAACATTAAAACTAAAGTTGAAGAAATTT CAAAAACAGGAGAATATTTACGAGTTAAACTTCCACTAAATCCAACTGCTAGGGAATTTCAGCCAGATGT AAAGTCTGAAGCAGCAGCTGAGGATGTGACGTCCATACCAGGACCTGCTGACTCTTCAGCACCAGCTGCT GAGGATCTGAAGCCCCAGCTGGACTCTGATAGCTCTTCTGGGTCAGCTTCAGAGGACAGCAGACTGGAAG TTGTCTCTCCTGACTTGCCTACACCACTCTGTGAGGATGCTTCTCCTTCCCCCACACCAGCTCCTGAAGA TGCCAAACCAACTTACTGGACTCAGTCACACCTGGTCACAGGATTCTGCACATACCTTCCTTTTCAAGGA TTTGGCATCGCCCAGTCTCGGCCAGCGTACATTAACATGGTACCAAGCCTGTCCCAGTTCACTAGCATTT ACACGCCTTTGGCCAACATTTCTTCTGAGTATCCAATGCCAAGATCCATGCCAGTGGTACCATCTTTTGT AGCCAGCGAGAGAGCAGACGGAAATGCCGCTGCATACTTTGAGAGTCATCGTCTGAACACTGAGAACGCC TCTGATGACCACACTGCCTCTGAAACACAGATCCTGGAGGGTCCTTTGGGAATGTGCGTAAAGTCCCAGA GCAGCACAGCTGATGCCCGGACAGCTCTGAGTGAGCCTGAAGGAAACAGCAGACACAGTGGGAGCTCTGA CAGCCTGTGGGAAGCTTCTCTAGAAAACGTCAGTGGAATAACAGATGTTCCACCAGCTCCGTCGGTTGCC ATACAGGTGTCCAGGAGCTTGATACATCAGGAAGTCAATACTGAGCCATATGCACCTTTTGAAAGACAAC AGGGGGACCTTTCCCAGAAGGAGAAGGAGTGCCACCTGCTCAGAGAGCAGCTTCAGGTGGCCTGTGAGGA GTGTGAGCAGATGGAACTCAGGAGCTCACAGGAGACCAGGGACCTGGAGGAGAAGCTGCAAAGGCACGCG GAGGAGAACAAGATTTCAAAGACGGAATTAGACTGGTTCCTCCAAGATTTGGACAGAGAGATTAAAAAGT GGCATCAAGAGAAAAAAGAAATCCAAGAAAGGTTAAAAGCCCTGAAGAAAAAAATTAAAAAGGTTATCAA TACCAGTGACATGTCTGCACAGAAAAATGATGGATTAGATAAAGAATGTGAATCACAGCCGGACCAGTCC CTTGGAATCAGCAGTGCACTCACAGATGAGAAGGCAAAAGTAGAGGAGTCTGTCAGGAAAGGGAAGGAGC TCTATGAAGAGAGTCAGCAGAGAGCTGTGGCTGCAGAGGTGTCTGTGCTTGAGAACTGGAAGGAGCGAGA AGTCTGTAAACTGCAGGGCGTGGCAACACAAGCAGAAGCCTGTCTTAAAAGCCTGAAGCTGATGAACAGT GACTCTGCAGCATATCCTGATGTGGAGTGTGATATACTTTCGTGGGAAACATTCCTTTCTACTGTAACAG AAGAAATTGAGAACACAAAGTGTCAGTTTGAAGAGCAAATTAAGGCTATTAAGAGTGGCTCTCGACTCAG TGACCTTTCCAGTGTACAGGTTTCTGAGCTTTTATTTCCTGCTTGTTCAATGATTCATCCTCAGTTACTC TCTGAGTCTTCAAGACATGAGGATCACGGGCTAGTGGCTTGTGTAGACAGCATGACTGGAGCAGTCCTCA ACGATCCGTATGTAGGGCCTGATAGTGGCTGCTCAGAGGAAGTTCCAGAACTGTCACTGGGATCGCCTAC CCATCATCCAGAAGGTGCTCAACAGTTAGAGGTTAAGAAGGCTAGCCAGGTGTCTCCATCAGAACAGAAC CCTGAGGCTGATGAAAAACCATCTGGGCAGGCCACGAGGTCAAGCCAGTCTCAAAAAAATCCTTTCAACA GTATTATTGAACACCTGTCAGTGATATTCCCATGTTATGCCAGCTCCGAGCTTTCTGGTTTTATTAAAAA AGTTAGAAACAAAAGCAAGAACTCATTCTCGGGATTAAGTATTGAGGAGATTGTTGAGAGAGTAACAGAA CACATTGTCGAGGAACAGAAGAAGAAGAAGCCAAATCCAGGAAAGGACAAGAAGACATCTGAGGCTCATC CCGCTGCCTCTGTGTCCAAGTCTTCCCCGAGTCCACCCTTGGCAGCTGCTGGACCATCAGCTAAAACCAA AGGCCAGAAAAAAGATGATGTCCCTGCACCAGATGGAAATTCCTGTCAAATATGCCATGAAATATTCAAG TCAAAAAACATGCGTGTGCTAAAATGTGGGCACAAGTTTCACAAAGGGTGCTTTAAGCAATGGCTGAAAG GGCAGAGCACGTGCCCCACCTGTGGCAGCAGTGACCTGCTGTCAGAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214244 representing NM_001108315 Red=Cloning site Green=Tags(s) MPGHRGAPHLPPWVCGLCSMDDFAEGGLSLADDILLEDYPYEDDCICTPDFTTDDYVRVTQLYYEGVGMQ YKDYAQSEKNLEYDICNIWCSKPLSILQDYCDAIKLYIFWPLLFQHQHSSIISRLHPCVEAIRSRAAEIS LKKLQHLELMEDIVDLAKKVANDSFLIEGLLKIGYKIENKILAMEDALNWIKYTGDVTILPKLGSVDSGW PMLSIFFTEYKYHITRVVTENCNLLEEFRRHSCMQCVKQGELMKMRGNEEFAKEKFEIAVIYYTRAIEYR PENHLLYGNRALCFLRMGQFRNALSDGKRAIVLKNTWPKGHYRYCDALSMLGEYDWALQANIKAQKLCKN DPEGIKDLIQQHIKLQKQIEDLQGRTSNRNPIKAFYESRAYIPRNSSAPAFRTSLNFVETERGFRKTKYR MANGGGHQNQKVADEALKGDDCDCHPEFLPPPSQPPRHKGKQKSRNNESEKPSSNSQVTLQVDLKSILEK QFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVY GLAVSLLGIGRPEELSEAENQFKRIIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKAKTLICRLP GILTWPTSNVIIEESKPEKVKVMLEKFVEECKFPPVPDAVCCYQKCRGFSKIQIYLTDPDFKGFIRISCC QYCKVEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVVKEKAPSR PVLKQKCSSLEKLRLKEDKKLKRKIQKQEAKKLAQERMEEDLRESNPPKPEEPEETVESAQSCQFLDDRI LQCIKQNADKIKSVVLNTSTLLKELLSWKVLSTEDYTTCFSSKNFVHEAVDYVIGHLIQEKNRVKTRIFL HVLSELKELDPKLAPWIQRLNSFGLDATGPFFTRYGASLKELDFNIVTFLWSEKYGHKLGSIEGKQLDYF CEPASVMEARCLIWLLEEHRDKFPALHSALDEFFDIMDSRCTVLRKQDSDEMPFGCIKVKNKGKKKKPKD SKPMLVGSGTASVTPSSETVTPEDHNRRNSDSAGPFAVPDHLRQDVEEFEALYDQHSSEYVVRNKKLWDI NPKQKCSTLYDYFSQLLEEHGPLDMSDRMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIAL KKVASRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFQPDVKSEAAAEDVTSIPGPADSSAPAA EDLKPQLDSDSSSGSASEDSRLEVVSPDLPTPLCEDASPSPTPAPEDAKPTYWTQSHLVTGFCTYLPFQG FGIAQSRPAYINMVPSLSQFTSIYTPLANISSEYPMPRSMPVVPSFVASERADGNAAAYFESHRLNTENA SDDHTASETQILEGPLGMCVKSQSSTADARTALSEPEGNSRHSGSSDSLWEASLENVSGITDVPPAPSVA IQVSRSLIHQEVNTEPYAPFERQQGDLSQKEKECHLLREQLQVACEECEQMELRSSQETRDLEEKLQRHA EENKISKTELDWFLQDLDREIKKWHQEKKEIQERLKALKKKIKKVINTSDMSAQKNDGLDKECESQPDQS LGISSALTDEKAKVEESVRKGKELYEESQQRAVAAEVSVLENWKEREVCKLQGVATQAEACLKSLKLMNS DSAAYPDVECDILSWETFLSTVTEEIENTKCQFEEQIKAIKSGSRLSDLSSVQVSELLFPACSMIHPQLL SESSRHEDHGLVACVDSMTGAVLNDPYVGPDSGCSEEVPELSLGSPTHHPEGAQQLEVKKASQVSPSEQN PEADEKPSGQATRSSQSQKNPFNSIIEHLSVIFPCYASSELSGFIKKVRNKSKNSFSGLSIEEIVERVTE HIVEEQKKKKPNPGKDKKTSEAHPAASVSKSSPSPPLAAAGPSAKTKGQKKDDVPAPDGNSCQICHEIFK SKNMRVLKCGHKFHKGCFKQWLKGQSTCPTCGSSDLLSEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108315
ORF Size	6000 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108315.1, NP_001101785.1
RefSeq Size	7364 bp
RefSeq ORF	6003 bp
Locus ID	360702
MW	225.9 kDa

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