Ttc3 (NM_001108315) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214244 representing NM_001108315 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGGCATCGGGGCGCTCCACACCTCCCTCCGTGGGTGTGTGGACTGTGCAGCATGGATGACTTTG CTGAGGGAGGTCTCAGTTTGGCAGATGATATCTTACTGGAAGATTACCCTTATGAGGATGACTGTATCTG TACTCCTGACTTTACCACTGATGATTATGTTCGAGTAACCCAGCTTTACTATGAAGGCGTGGGTATGCAA TATAAAGATTATGCCCAAAGTGAGAAAAATTTAGAGTATGACATCTGCAATATATGGTGCAGTAAGCCAC TGTCCATCCTGCAAGATTACTGTGATGCCATTAAGCTGTACATCTTCTGGCCACTTCTCTTTCAACATCA GCACAGTTCTATAATATCAAGATTGCACCCCTGTGTAGAAGCCATCCGTTCTCGTGCTGCTGAGATAAGT TTGAAGAAATTACAACATCTTGAGTTGATGGAAGACATTGTGGATTTGGCAAAGAAAGTTGCAAATGATT CATTCCTTATTGAAGGCTTATTGAAAATTGGTTATAAAATAGAAAATAAAATCTTGGCAATGGAAGATGC TTTAAATTGGATAAAATACACGGGTGATGTAACAATTCTACCTAAATTAGGATCAGTTGACAGTGGCTGG CCCATGTTAAGTATTTTCTTTACTGAATATAAGTACCATATTACTAGAGTTGTAACTGAAAATTGCAACT TGCTAGAAGAATTTAGAAGGCATAGTTGCATGCAGTGTGTGAAGCAAGGAGAACTCATGAAAATGAGAGG AAATGAAGAGTTTGCAAAGGAAAAGTTTGAAATAGCTGTTATTTATTACACCAGAGCCATTGAATATAGA CCTGAAAACCATCTTCTTTATGGTAACCGAGCTCTCTGTTTTCTTCGTATGGGGCAGTTTAGAAATGCAC TTAGTGATGGAAAGAGGGCCATTGTTTTGAAGAACACCTGGCCGAAGGGTCATTATCGTTATTGTGATGC TCTTTCTATGCTGGGGGAATATGACTGGGCCCTGCAAGCAAACATAAAAGCTCAAAAACTCTGTAAAAAT GACCCTGAGGGAATCAAGGATCTAATTCAGCAGCATATAAAGTTACAAAAACAAATAGAAGACCTACAAG GTCGGACATCAAATAGGAATCCAATTAAAGCTTTTTATGAAAGCAGGGCTTACATACCTAGAAACTCATC AGCACCTGCTTTTAGAACATCACTTAACTTTGTGGAAACAGAAAGAGGTTTCAGGAAAACTAAGTACAGA ATGGCAAATGGCGGTGGCCATCAGAATCAAAAGGTGGCAGATGAGGCTTTGAAGGGGGACGATTGTGACT GTCATCCTGAATTTCTGCCACCTCCAAGTCAGCCTCCAAGACATAAAGGAAAACAAAAGTCACGGAACAA TGAATCAGAAAAGCCCAGTTCTAATTCTCAAGTGACTTTACAAGTAGATTTGAAAAGCATTTTGGAGAAA CAATTTTCAAAATCTTCCAGAGCTGCACACCAGGATTTTGCTAACATCATGAAAATGTTAAGAAGCTTGA TTCAGGATGGGTACACGGCCTTGTTGGAGCAGCGCTGCCGCAGTGCTGCCCAGGCCTTTACAGAGTTACT CAATGGCTTAGATCCTCAGAAAATAAAGCAATTGAACCTGGCCATGATTAATTATGTATTAGTAGTCTAT GGACTTGCTGTTTCTCTCCTTGGAATAGGACGACCTGAGGAACTGTCTGAAGCCGAAAACCAGTTTAAGA GGATCATCGAACACTATCCCAATGAGGGGCTTGACTGCTTGGCCTACTGTGGAATAGGAAAAGTATATTT GAAAAAAAACAGATTTCTCGAAGCTCTTAATCACTTTGAAAAAGCAAAAACTTTAATTTGTCGTCTCCCT GGAATTCTAACTTGGCCCACAAGTAATGTGATTATTGAAGAATCTAAGCCAGAAAAGGTCAAGGTGATGT TAGAGAAGTTTGTTGAAGAATGCAAGTTTCCTCCTGTGCCGGATGCTGTTTGTTGCTATCAGAAGTGCCG TGGGTTTTCCAAAATCCAGATTTACCTAACTGACCCAGACTTCAAGGGTTTTATACGAATTAGCTGTTGC CAATACTGTAAAGTAGAATTTCACATGAACTGCTGGAAAAAGTTAAAAACAACAACTTTTAATGATAAAA TTGACAAGGATTTTCTACAAGGAATTTGTCTCACCCCTGACTGTGAAGGAATCATTTCTAAGATTATCAT CTTTAGCAGTGGTGGCCAAGTTAAATGTGAATTTGAACACAAGGTCGTAAAAGAAAAGGCTCCTTCAAGA CCTGTTCTGAAACAGAAATGTTCTAGTCTAGAGAAGTTAAGACTGAAAGAAGACAAAAAATTGAAGAGAA AGATCCAAAAACAAGAGGCAAAAAAGTTGGCACAAGAAAGAATGGAAGAGGACTTAAGAGAAAGTAATCC ACCTAAACCTGAAGAGCCGGAAGAAACTGTAGAAAGCGCTCAGAGTTGTCAGTTTCTTGATGACAGAATC CTTCAGTGCATAAAGCAGAATGCTGACAAGATTAAATCTGTTGTGTTGAACACTTCCACGCTTCTCAAAG AGTTGCTGTCTTGGAAGGTTCTGAGCACAGAAGACTACACAACATGTTTTTCTAGCAAAAATTTTGTTCA TGAAGCAGTGGACTATGTTATCGGTCACTTGATTCAAGAAAAGAACAGAGTAAAGACACGGATATTTCTG CATGTTTTGAGTGAGCTTAAAGAGTTAGACCCCAAGTTAGCTCCCTGGATCCAGAGACTGAATAGCTTTG GCTTAGATGCCACAGGACCCTTTTTTACTCGCTATGGAGCATCTCTCAAAGAGCTTGATTTTAACATTGT GACTTTCCTTTGGAGTGAGAAATATGGCCATAAACTAGGTTCTATAGAAGGAAAGCAACTTGATTACTTC TGTGAGCCAGCATCAGTAATGGAAGCACGCTGCTTAATATGGCTGCTGGAAGAGCACAGGGACAAGTTCC CCGCCTTGCACAGTGCTTTAGACGAGTTCTTTGATATAATGGACAGCCGATGCACTGTGCTAAGGAAGCA AGACAGTGATGAAATGCCGTTTGGTTGTATCAAGGTAAAAAACAAAGGCAAGAAAAAGAAGCCAAAAGAT TCAAAGCCGATGTTAGTGGGATCTGGAACAGCATCAGTAACACCAAGTAGTGAGACTGTCACTCCAGAAG ACCATAACAGGCGCAATTCAGATTCTGCAGGACCATTTGCAGTGCCTGACCACCTTCGGCAAGATGTGGA AGAGTTCGAAGCTCTTTACGACCAGCACAGCAGCGAGTATGTTGTCCGAAATAAGAAGCTGTGGGATATT AACCCCAAACAGAAATGTTCAACTCTGTATGATTACTTCTCTCAGTTGCTGGAAGAGCACGGCCCCTTGG ATATGAGTGATAGGATGTTCTCTGAGGAATATGAGTTCTTCCCTGAAGAAACTCGCCAGATACTGGAGAA GGCAGGAGGGCTGAAGTCGTTCCTCCTGGGCTGTCCTCGATTTGTTGTGATTGACAACTGCATTGCATTG AAAAAAGTTGCCTCAAGACTCAAGAAAAAAAGAAAGAAGAAAAACATTAAAACTAAAGTTGAAGAAATTT CAAAAACAGGAGAATATTTACGAGTTAAACTTCCACTAAATCCAACTGCTAGGGAATTTCAGCCAGATGT AAAGTCTGAAGCAGCAGCTGAGGATGTGACGTCCATACCAGGACCTGCTGACTCTTCAGCACCAGCTGCT GAGGATCTGAAGCCCCAGCTGGACTCTGATAGCTCTTCTGGGTCAGCTTCAGAGGACAGCAGACTGGAAG TTGTCTCTCCTGACTTGCCTACACCACTCTGTGAGGATGCTTCTCCTTCCCCCACACCAGCTCCTGAAGA TGCCAAACCAACTTACTGGACTCAGTCACACCTGGTCACAGGATTCTGCACATACCTTCCTTTTCAAGGA TTTGGCATCGCCCAGTCTCGGCCAGCGTACATTAACATGGTACCAAGCCTGTCCCAGTTCACTAGCATTT ACACGCCTTTGGCCAACATTTCTTCTGAGTATCCAATGCCAAGATCCATGCCAGTGGTACCATCTTTTGT AGCCAGCGAGAGAGCAGACGGAAATGCCGCTGCATACTTTGAGAGTCATCGTCTGAACACTGAGAACGCC TCTGATGACCACACTGCCTCTGAAACACAGATCCTGGAGGGTCCTTTGGGAATGTGCGTAAAGTCCCAGA GCAGCACAGCTGATGCCCGGACAGCTCTGAGTGAGCCTGAAGGAAACAGCAGACACAGTGGGAGCTCTGA CAGCCTGTGGGAAGCTTCTCTAGAAAACGTCAGTGGAATAACAGATGTTCCACCAGCTCCGTCGGTTGCC ATACAGGTGTCCAGGAGCTTGATACATCAGGAAGTCAATACTGAGCCATATGCACCTTTTGAAAGACAAC AGGGGGACCTTTCCCAGAAGGAGAAGGAGTGCCACCTGCTCAGAGAGCAGCTTCAGGTGGCCTGTGAGGA GTGTGAGCAGATGGAACTCAGGAGCTCACAGGAGACCAGGGACCTGGAGGAGAAGCTGCAAAGGCACGCG GAGGAGAACAAGATTTCAAAGACGGAATTAGACTGGTTCCTCCAAGATTTGGACAGAGAGATTAAAAAGT GGCATCAAGAGAAAAAAGAAATCCAAGAAAGGTTAAAAGCCCTGAAGAAAAAAATTAAAAAGGTTATCAA TACCAGTGACATGTCTGCACAGAAAAATGATGGATTAGATAAAGAATGTGAATCACAGCCGGACCAGTCC CTTGGAATCAGCAGTGCACTCACAGATGAGAAGGCAAAAGTAGAGGAGTCTGTCAGGAAAGGGAAGGAGC TCTATGAAGAGAGTCAGCAGAGAGCTGTGGCTGCAGAGGTGTCTGTGCTTGAGAACTGGAAGGAGCGAGA AGTCTGTAAACTGCAGGGCGTGGCAACACAAGCAGAAGCCTGTCTTAAAAGCCTGAAGCTGATGAACAGT GACTCTGCAGCATATCCTGATGTGGAGTGTGATATACTTTCGTGGGAAACATTCCTTTCTACTGTAACAG AAGAAATTGAGAACACAAAGTGTCAGTTTGAAGAGCAAATTAAGGCTATTAAGAGTGGCTCTCGACTCAG TGACCTTTCCAGTGTACAGGTTTCTGAGCTTTTATTTCCTGCTTGTTCAATGATTCATCCTCAGTTACTC TCTGAGTCTTCAAGACATGAGGATCACGGGCTAGTGGCTTGTGTAGACAGCATGACTGGAGCAGTCCTCA ACGATCCGTATGTAGGGCCTGATAGTGGCTGCTCAGAGGAAGTTCCAGAACTGTCACTGGGATCGCCTAC CCATCATCCAGAAGGTGCTCAACAGTTAGAGGTTAAGAAGGCTAGCCAGGTGTCTCCATCAGAACAGAAC CCTGAGGCTGATGAAAAACCATCTGGGCAGGCCACGAGGTCAAGCCAGTCTCAAAAAAATCCTTTCAACA GTATTATTGAACACCTGTCAGTGATATTCCCATGTTATGCCAGCTCCGAGCTTTCTGGTTTTATTAAAAA AGTTAGAAACAAAAGCAAGAACTCATTCTCGGGATTAAGTATTGAGGAGATTGTTGAGAGAGTAACAGAA CACATTGTCGAGGAACAGAAGAAGAAGAAGCCAAATCCAGGAAAGGACAAGAAGACATCTGAGGCTCATC CCGCTGCCTCTGTGTCCAAGTCTTCCCCGAGTCCACCCTTGGCAGCTGCTGGACCATCAGCTAAAACCAA AGGCCAGAAAAAAGATGATGTCCCTGCACCAGATGGAAATTCCTGTCAAATATGCCATGAAATATTCAAG TCAAAAAACATGCGTGTGCTAAAATGTGGGCACAAGTTTCACAAAGGGTGCTTTAAGCAATGGCTGAAAG GGCAGAGCACGTGCCCCACCTGTGGCAGCAGTGACCTGCTGTCAGAAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108315
Insert Size	6003 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108315.1, NP_001101785.1
RefSeq Size	7364 bp
RefSeq ORF	6003 bp
Locus ID	360702

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.