Cntnap1 (NM_032061) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Caspr
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214100 representing NM_032061 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGTCTCCGGCTTTTCAGCATTCTGCTCGCCGCTGTGGTCTCTGGAGCCCAGGGCTGGGGCTACT ATGGCTGCAATGAGGAGCTGGTGGGGCCTCTGTATGCACGGTCTCTGGGTGCTTCCTCCTACTATGGACT CTTTACCACAGCCCGCTTTGCCCGGCTACACGGCATCAGTGGATGGTCGCCCCGGATTGGGGACCCGAAT CCCTGGCTCCAGATAGACTTAATGAAGAAGCATCGAATCCGGGCTGTGGCCACACAGGGAGCCTTTAATT CTTGGGATTGGGTCACACGTTACATGCTGCTCTACGGGGACCGTGTGGACAGCTGGACACCATTCTACCA ACAAGGGCACAACGCGACCTTCTTCGGTAATGTCAACGACTCGGCGGTGGTACGCCATGACCTGCACTAC CATTTTACGGCTCGCTACATCCGCATCGTGCCACTGGCCTGGAACCCCCGCGGCAAGATTGGCTTGAGGC TGGGCATCTACGGTTGTCCCTACACGTCCAACATCCTGTATTTTGACGGCGATGATGCCATTTCATACCG CTTCCAGCGAGGGGCCAGTCAAAGTCTTTGGGACGTGTTCGCTTTTAGTTTCAAGACAGAGGAGAAGGAC GGGCTGCTGTTGCACACCGAAGGCTCCCAGGGGGATTATGTGACGCTTGAACTGCAAGGAGCACACCTGC TGCTGCACATGAGCCTGGGCAGCAGCCCCATCCAGCCGAGACCTGGTCACACCACGGTGAGCGCTGGTGG CGTACTTAATGACCTAAGCTGGCATTATGTGCGGGTGGACCGATACGGCCGAGAAGCAAATCTCACCCTG GATGGTTACGTACATCGCTTTGTGCTCAACGGCGACTTTGAAAGGCTGAATCTCGAAAATGAGATATTCA TCGGAGGTCTAGTGGGCGCAGCGCGTAAGAACCTGGCCTACCGCCATAACTTCCGTGGCTGTATAGAAAA CGTGATCTACAACCGGATCAACATAGCTGAAATGGCAGTGCAGCGCCATTCGCGGATCACCTTCGAGGGT AATGTGGCTTTCCGGTGCTTGGATCCCGTTCCACACCCCATCAACTTCGGAGGCCCTCACAACTTCGTCC AAGTGCCTGGCTTTCCACGTCGAGGCCGCCTTGCTGTCTCCTTCCGCTTCCGCACCTGGGACCTCACAGG GCTGCTCCTTTTCTCCCGCTTGGGGGATGGGCTGGGTCATGTGGAGCTGATGCTTAGTGAAGGGCAAGTC AATGTATCCATCGCGCAGACTGGCCGCAAGAAGCTTCAGTTTGCTGCGGGGTACCGCCTGAATGATGGCT TCTGGCATGAGGTGAACTTTGTGGCACAGGAAAACCATGCGGTCATCAGTATTGATGATGTGGAGGGGGC AGAGGTCAGGGTATCATACCCACTGCTGATCCGCACAGGGACTTCATACTTCTTTGGTGGTTGTCCCAAA CCAGCCAGTCGATGGGGCTGCCACTCCAACCAGACAGCATTCCATGGCTGCATGGAGCTGCTCAAGGTGG ATGGTCAACTGGTCAACCTCACTCTGGTAGAGTTTCGGAAGCTTGGTTACTTTGCTGAGGTCCTCTTTGA CACATGTGGCATCACAGACAGATGCAGCCCTAATATGTGTGAGCATGATGGGCGCTGCTACCAGTCTTGG GATGACTTCATCTGCTACTGCGAACTCACCGGCTACAAGGGAGTTACCTGCCATGAACCATTGTATAAGG AGTCCTGTGAAGCCTATCGCCTCAGCGGGAAATATTCTGGAAATTACACCATTGATCCTGATGGCAGTGG ACCCCTGAAACCATTTGTAGTGTATTGTGATATCCGAGAGAACCGAGCATGGACAGTTGTGCGACATGAC AGGCTATGGACCACTCGAGTGACAGGTTCCAGCATGGACCGGCCCTTTCTGGGGGCCATCCAATACTGGA ATGCCTCCTGGGAGGAAGTCAGTGCTCTGGCCAATGCTTCCCAGCACTGTGAGCAGTGGATCGAGTTCTC CTGCTACAATTCCCGGCTGCTCAACACTGCAGGAGGCTACCCCTACAGCTTTTGGATTGGCCGAAATGAA GAACAGCATTTCTACTGGGGAGGCTCCCAGCCTGGGATCCAACGCTGTGCCTGTGGGCTGGACCAGAGCT GTATAGACCCTGCACTGCACTGCAACTGCGATGCTGACCAGCCACAGTGGAGAACAGACAAGGGGCTCCT GACCTTTGTGGACCATCTGCCTGTCACTCAGGTAGTGATAGGTGACACAAACCGCTCCAGCTCTGAAGCT CAGTTCTTCCTGAGGCCTCTGCGCTGTTATGGTGACCGCAATTCCTGGAACACTATCTCCTTCCGCACTG GAGCTGCACTGCGTTTCCCTCCAATCCGTGCCAACCACAGCCTTGATGTCTCCTTCTACTTCAGGACCTC GGCTCCCTCAGGAGTCTTCCTAGAGAACATGGGGGGTCCTTTCTGCCAGTGGCGCCGACCTTACGTGAGA GTGGAGCTCAACACATCCCGGGATGTGGTCTTTGCCTTTGATATTGGCAATGGGGATGAGAACCTGACAG TGCACTCAGATGACTTCGAGTTCAATGATGACGAGTGGCATTTGGTCCGGGCTGAAATCAACGTGAAGCA GGCCCGGCTGCGAGTGGACCATCGGCCCTGGGTGCTAAGGCCCATGCCCCTGCAGACGTACATCTGGCTG GAGTATGACCAACCCCTCTATGTGGGATCTGCAGAGCTTAAGAGGCGCCCATTTGTGGGGTGCTTGAGGG CCATGCGTTTGAATGGAGTGACTCTGAACTTGGAGGGTCGTGCCAATGCCTCCGAGGGCACCTTCCCCAA CTGCACGGGCCACTGCACCCACCCCCGGTTCCCCTGTTTCCACGGAGGACGCTGTGTGGAGCGATACAGC TACTACACGTGTGACTGTGACCTCACAGCTTTTGATGGACCATATTGTAATCACGATATTGGTGGATTCT TTGAGACTGGCACATGGATGCGCTATAACCTCCAGTCAGCACTGCGTTCTGCGGCCCAGGAGTTCTCTCA CATGCTGAGCCGGCCGGTACCGGGCTATGAGCCTGGCTATATCCCAGGCTACGACACTCCTGGTTACGTG CCTGGGTACCATGGTCCTGGGTACCGCCTACCCGACTACCCAAGGCCTGGCCGGCCAGTGCCCGGATACC GGGGGCCCGTGTACAATGTTACTGGAGAGGAGGTCTCCTTTAGCTTCAGCACCAGCTCTGCTCCTGCAGT CCTGCTCTACGTCAGCTCCTTTGTGCGTGACTACATGGCCGTGCTCATCAAGGAAGATGGGACCCTACAG CTTCGCTATCAGCTGGGCACCAGTCCCTATGTGTACCAGCTAACCACCCGGCCAGTGACCGATGGCCAAC CCCATAGTGTCAACATCACCCGGGTCTACCGAAACCTCTTTATCCAGGTGGACTACTTCCCGCTGACAGA ACAGAAGTTCTCTCTCCTGGTGGACAGCCAGCTGGACTCCCCCAAGGCCTTGTATCTAGGGCGTGTGATG GAGACAGGAGTCATTGACCCAGAGATTCAGCGGTACAACACCCCAGGTTTCTCAGGCTGCCTGTCTGGTG TCCGGTTCAACAATGTGGCTCCTCTCAAGACCCATTTCCGAACCCCTCGCCCCATGACTGCTGAGCTGGC GGAGGCCATGCGGGTTCAAGGAGAACTGTCGGAGTCTAACTGTGGCGCTATGCCACGCCTTGTCTCCGAG GTGCCACCAGAGCTTGATCCCTGGTACCTGCCCCCAGATTTCCCATACTACCATGACGACGGATGGATTG CCATACTCTTAGGTTTTTTGGTGGCCTTCCTGCTGCTGGGGCTTGTGGGAATGCTGGTGCTGTTCTATCT GCAAAATCATCGATACAAGGGCTCCTATCACACCAACGAGCCCAAGGCCACCCATGATTCCCACCCTGGC GGCAAAGCTCCCCTACCTCCCTCAGGCCCTGCCCAGGCCCCTGCCCCCACTCCAGCTCCCACCCAGGTTC CGACCCCAGCCCCAGCCCCAGCCTCTGGCCCAGGCCCCAGGGACCAGAACCTCCCCCAGATCTTGGAGGA GTCCAGGTCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214100 representing NM_032061 Red=Cloning site Green=Tags(s) MMSLRLFSILLAAVVSGAQGWGYYGCNEELVGPLYARSLGASSYYGLFTTARFARLHGISGWSPRIGDPN PWLQIDLMKKHRIRAVATQGAFNSWDWVTRYMLLYGDRVDSWTPFYQQGHNATFFGNVNDSAVVRHDLHY HFTARYIRIVPLAWNPRGKIGLRLGIYGCPYTSNILYFDGDDAISYRFQRGASQSLWDVFAFSFKTEEKD GLLLHTEGSQGDYVTLELQGAHLLLHMSLGSSPIQPRPGHTTVSAGGVLNDLSWHYVRVDRYGREANLTL DGYVHRFVLNGDFERLNLENEIFIGGLVGAARKNLAYRHNFRGCIENVIYNRINIAEMAVQRHSRITFEG NVAFRCLDPVPHPINFGGPHNFVQVPGFPRRGRLAVSFRFRTWDLTGLLLFSRLGDGLGHVELMLSEGQV NVSIAQTGRKKLQFAAGYRLNDGFWHEVNFVAQENHAVISIDDVEGAEVRVSYPLLIRTGTSYFFGGCPK PASRWGCHSNQTAFHGCMELLKVDGQLVNLTLVEFRKLGYFAEVLFDTCGITDRCSPNMCEHDGRCYQSW DDFICYCELTGYKGVTCHEPLYKESCEAYRLSGKYSGNYTIDPDGSGPLKPFVVYCDIRENRAWTVVRHD RLWTTRVTGSSMDRPFLGAIQYWNASWEEVSALANASQHCEQWIEFSCYNSRLLNTAGGYPYSFWIGRNE EQHFYWGGSQPGIQRCACGLDQSCIDPALHCNCDADQPQWRTDKGLLTFVDHLPVTQVVIGDTNRSSSEA QFFLRPLRCYGDRNSWNTISFRTGAALRFPPIRANHSLDVSFYFRTSAPSGVFLENMGGPFCQWRRPYVR VELNTSRDVVFAFDIGNGDENLTVHSDDFEFNDDEWHLVRAEINVKQARLRVDHRPWVLRPMPLQTYIWL EYDQPLYVGSAELKRRPFVGCLRAMRLNGVTLNLEGRANASEGTFPNCTGHCTHPRFPCFHGGRCVERYS YYTCDCDLTAFDGPYCNHDIGGFFETGTWMRYNLQSALRSAAQEFSHMLSRPVPGYEPGYIPGYDTPGYV PGYHGPGYRLPDYPRPGRPVPGYRGPVYNVTGEEVSFSFSTSSAPAVLLYVSSFVRDYMAVLIKEDGTLQ LRYQLGTSPYVYQLTTRPVTDGQPHSVNITRVYRNLFIQVDYFPLTEQKFSLLVDSQLDSPKALYLGRVM ETGVIDPEIQRYNTPGFSGCLSGVRFNNVAPLKTHFRTPRPMTAELAEAMRVQGELSESNCGAMPRLVSE VPPELDPWYLPPDFPYYHDDGWIAILLGFLVAFLLLGLVGMLVLFYLQNHRYKGSYHTNEPKATHDSHPG GKAPLPPSGPAQAPAPTPAPTQVPTPAPAPASGPGPRDQNLPQILEESRSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_032061
ORF Size	4143 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_032061.2, NP_114450.1
RefSeq Size	5379 bp
RefSeq ORF	4146 bp
Locus ID	84008
UniProt ID	P97846
MW	155.9 kDa
Gene Summary	forms a complex with contactin; may mediate communication between glial cells and neurons during development [RGD, Feb 2006]

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