Nfasc (NM_053909) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214024 representing NM_053909 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCAACAACCCCTATA ATGACTCGTCTTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCGGAAAGAACACCTAGCTT CATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATT GCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCA AGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGG CTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGA ACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGGCACCACCCAACCCCAACCG TGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAAC ACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGC TGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGG GTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCAC GTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG CCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG GATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCC TCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATTGGAAACAGGATGAAAAAGG AAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCAC CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGGCCAGACCGACCCAGGGACCTG GAGCTCACTGACCTGGCGGAAAGGAGTGTGAGGCTGACCTGGATCCCGGGGGATGATAACAACAGTCCCA TCACAGACTACGTTGTCCAGTTTGAAGAGGACCAGTTCCAGCCAGGAGTCTGGCATGACCACTCCAAGTT CCCAGGCAGTGTCAACTCAGCCGTCCTCCATCTGTCCCCGTATGTCAACTATCAGTTCCGAGTCATCGCT GTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACAAGCGGGGCACCCCCTG AATCCAACCCCAGTGATGTGAAGGGCGAAGGGACACGAAAAAACAATATGGAGATCACATGGACGCCTAT GAATGCTACCTCCGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGCGACGGAGAGAAACCCGAGAG ACTTGGAACAATGTCACCGTGTGGGGCTCTCGCTATGTGGTGGGGCAGACCCCTGTCTACGTACCCTATG AGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCGGAGCCTGAAACCGTCATTGGGTACTCGGG GGAAGATTATCCCAGGGCTGCACCCACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTT CAGTGGAACCGCGTCTACCCTGACACGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGG AAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGCGACCGCCC CCGGGGCGTGGTGGGTCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGA GGTGATGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCGGAAGGAGTACCCAGTGCCCCCAGGCGTT TCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGAT CCTGATTGGATACACGCTCAGATACGTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAAC TTCTCTCCCAATCAGACCAAGTTCTCGGTGCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCA GTGCCAGGACGCAGGTGGGCTCTGGAGAAGCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTAC TCCAACTGCAGCTTACACCAACAACCAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGT GCCATTGCCCTTCTGGTGCTGATCCTGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACC CAGTGCGAGAAAAGAAGGATGTCCCCTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTA CAGTGACGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAG AGTGACGACAGCCTAGTGGACTACGGCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTG GCCAGTACACTGTCAGAAAGGACAAGGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGT CAATGCCATCTATTCCCTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214024 representing NM_053909 Red=Cloning site Green=Tags(s) MARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWT RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR FHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECI ASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW LDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN TSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDP SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDL ELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIA VNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRE TWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISL QWNRVYPDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGR GDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVEN FSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMC AIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQE SDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053909
ORF Size	3522 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053909.2, NP_446361.1
RefSeq Size	9627 bp
RefSeq ORF	3525 bp
Locus ID	116690
UniProt ID	P97685
MW	132.2 kDa
Gene Summary	This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]

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