Nfasc (NM_053909) Rat Untagged Clone
CAT#: RN214024
Nfasc (untagged ORF) - Rat neurofascin (Nfasc), (10 ug)
CNY 18,910.00
货期*
7周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | NF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN214024 representing NM_053909
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCAACAACCCCTATA ATGACTCGTCTTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCGGAAAGAACACCTAGCTT CATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATT GCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCA AGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGG CTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGA ACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGGCACCACCCAACCCCAACCG TGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAAC ACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGC TGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGG GTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCAC GTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG CCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG GATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCC TCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATTGGAAACAGGATGAAAAAGG AAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCAC CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGGCCAGACCGACCCAGGGACCTG GAGCTCACTGACCTGGCGGAAAGGAGTGTGAGGCTGACCTGGATCCCGGGGGATGATAACAACAGTCCCA TCACAGACTACGTTGTCCAGTTTGAAGAGGACCAGTTCCAGCCAGGAGTCTGGCATGACCACTCCAAGTT CCCAGGCAGTGTCAACTCAGCCGTCCTCCATCTGTCCCCGTATGTCAACTATCAGTTCCGAGTCATCGCT GTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACAAGCGGGGCACCCCCTG AATCCAACCCCAGTGATGTGAAGGGCGAAGGGACACGAAAAAACAATATGGAGATCACATGGACGCCTAT GAATGCTACCTCCGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGCGACGGAGAGAAACCCGAGAG ACTTGGAACAATGTCACCGTGTGGGGCTCTCGCTATGTGGTGGGGCAGACCCCTGTCTACGTACCCTATG AGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCGGAGCCTGAAACCGTCATTGGGTACTCGGG GGAAGATTATCCCAGGGCTGCACCCACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTT CAGTGGAACCGCGTCTACCCTGACACGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGG AAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGCGACCGCCC CCGGGGCGTGGTGGGTCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGA GGTGATGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCGGAAGGAGTACCCAGTGCCCCCAGGCGTT TCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGAT CCTGATTGGATACACGCTCAGATACGTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAAC TTCTCTCCCAATCAGACCAAGTTCTCGGTGCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCA GTGCCAGGACGCAGGTGGGCTCTGGAGAAGCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTAC TCCAACTGCAGCTTACACCAACAACCAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGT GCCATTGCCCTTCTGGTGCTGATCCTGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACC CAGTGCGAGAAAAGAAGGATGTCCCCTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTA CAGTGACGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAG AGTGACGACAGCCTAGTGGACTACGGCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTG GCCAGTACACTGTCAGAAAGGACAAGGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGT CAATGCCATCTATTCCCTGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_053909 |
Insert Size | 3525 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053909.2, NP_446361.1 |
RefSeq Size | 9627 bp |
RefSeq ORF | 3525 bp |
Locus ID | 116690 |
UniProt ID | P97685 |
Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009] Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences in the N-terminal and central regions, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR214024 | Nfasc (Myc-DDK-tagged ORF) - Rat neurofascin (Nfasc), (10 ug) |
CNY 18,910.00 |
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