Cbln1 (NM_001109127) Rat Tagged Lenti ORF Clone
CAT#: RR213969L4
- LentiORF®
Lenti ORF clone of Cbln1 (mGFP-tagged ORF) - Rat cerebellin 1 precursor (Cbln1), (10 ug)
"NM_001109127" in other vectors (3)
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CNY 6,650.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | mGFP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR213969).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001109127 |
ORF Size | 579 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001109127.1, NP_001102597.1 |
RefSeq Size | 2444 bp |
RefSeq ORF | 582 bp |
Locus ID | 498922 |
UniProt ID | P63182 |
Gene Summary | Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the matching and maintenance of pre- and post-synaptic elements at parallel fiber-Purkinje cell synapses, the establishment of the proper pattern of climbing fiber-Purkinje cell innervation, and induction of long-term depression at parallel fiber-Purkinje cell synapses. Plays a role as a synaptic organizer that acts bidirectionally on both pre- and post-synaptic components. On the one hand induces accumulation of synaptic vesicles in the pre-synaptic part by binding with NRXN1 and in other hand induces clustering of GRID2 and its associated proteins at the post-synaptic site through association of GRID2. NRXN1-CBLN1-GRID2 complex directly induces parallel fiber protrusions that encapsulate spines of Purkinje cells leading to accumulation of GRID2 and synaptic vesicles. Required for CBLN3 export from the endoplasmic reticulum and secretion (By similarity). NRXN1-CBLN1-GRID2 complex mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN213969 | Cbln1 (untagged ORF) - Rat cerebellin 1 precursor (Cbln1), (10 ug) |
CNY 3,990.00 |
|
RR213969 | Cbln1 (Myc-DDK-tagged ORF) - Rat cerebellin 1 precursor (Cbln1), (10 ug) |
CNY 3,990.00 |
|
RR213969L3 | Lenti ORF clone of Cbln1 (Myc-DDK-tagged ORF) - Rat cerebellin 1 precursor (Cbln1), (10 ug) |
CNY 6,080.00 |