Adcy10 (NM_021684) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sac
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213767 representing NM_021684 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCCCGAAGGCAGGAATTACAGGACAGGGCAATCGTCAAGATAGCTGCTCACTTACCGGACCTCA TTGTCTATGGAGATTTCTCTCCCGAGCGGCCGTCAGTGAAATGTTTTGATGGAGTTCTGATGTTTGTCGA TATTTCAGGCTTTACTGCAATGACTGAGAAGTTCAGCACAGCCATGTACATGGACCGAGGAGCCGAGCAG CTGGTGGAGATCCTCAACTACTACATAAGTGCGATAGTGGAGAAAGTACTGATTTTTGGAGGAGACATCC TAAAATTTGCAGGTGACGCCTTGTTGGCCCTGTGGAAAGTGGAACGAAAGCAACTGAAGAATATCATCAC GGTGGTAATTAAATGCAGCCTGGAGATTCATGGCTTGTTTGAAGCCAAGGAGGTTGAAGAAGGCCTGGAT ATTCGAGTTAAGATAGGACTGGCTGCTGGCCACATCACCATGTTGGTCTTTGGGGATGAAACACGGAACT ACTTCCTGGTGATTGGCCAAGCGGTGGATGATGTACGCCTTGCTCAGAACATGGCTCAGATGAATGATGT TATTCTGTCACCAAACTGCTGGCAGCTCTGTGATCGGAGCATGATTGAAATCGAGAGGATTCCGGATCAG AGAGCAGTTAAGGTTAGCTTCTTAAAACCACCCCCAACTTTTAACTTCGACGAGTTTTTTGCCAAGTGTA TGGCCTTCATGGATTATTATCCTTCTGGTGACCACAAAAACTTCCTAAGGCTTGCCTGCATGCTGGAGTC TGATCCTGAACTCGAGTTGTCTCTACAAAAGTATGTGATGGAAATCATTTTGAAGCAGATTGATGACAAG CAGCTTCGGGGCTATTTATCTGAGCTTCGTCCTGTGACGATCGTGTTTGTGAACTTGATGTTTAAAGAGC AAGACAAAGCAGAAGTCATAGGATCAGCCATCCAAGCTGCCTGTGTGCACATCACTTCCGTCTTGAAGGT CTTCCGAGGCCAGATCAATAAGGTCTTCATGTTTGATAAGGGCTGCTCCTTCCTCTGTGTCTTCGGTTTC CCTGGGGAAAAGGCCCCTGACGAGATCACTCACGCTTTGGAAAGTGCCGTGGATATATTCGACTTCTGCT CTCAGGTCCACAAAATCCGTACTGTCTCCATCGGCGTCGCCAGTGGGATTGTCTTCTGTGGGATCGTTGG ACACACTGTGAGACACGAGTACACAGTCATTGGCCAAAAGGTCAATATTGCTGCCAGGATGATGATGTAT TACCCAGGCATCGTGACCTGCGACTCTGTCACATACGATGGCAGCAACCTGCCAGCCTACTTTTTTAAAG AGCTTCCAAAGAAAGTCATGAAAGGAGTTGCGGATCCCGGACCAGTGTATCAGTGTCTGGGCCTCAATGA GAAAGTCATGTTTGGTATGGCCTATCTCATCTGCAACAGATATGAGGGCTACCCTTTGCTGGGTCGTGTT AGGGAGATCGACTATTTCATGTCTACTATGAAGGACTTTCTGATGACGAACTGCAGCCGAGTTCTAATGT ATGAAGGATTGCCAGGATATGGGAAAAGCCAGGTACTTATGGAAATCGAGTATCTGGCCTCCCAGCATGA GAACCATAGGGCTGTTGCTATTGCACTGACTAAGATCAGCTTCCATCAAAATTTTTACACTATCCAGATA CTCATGGCTAACGTACTAGGTCTGGATACTTGTAAACATTACAAAGAACGACAGACCAATCTTCAAAATA GAGTCAAGACGCTGTTGGATGATAAATACCACTGTCTCCTTAACGACATCTTCCATGTTCAGTTCCCCGT TTCCCGGGAGATGTCCAGGATGAGCAAGATAAGAAAGCAGAAGCAACTGGAAGCTCTGTTTATGAAGATC CTGGAGCAAACAGTGAGGGAAGAAAGGATTATCTTCATCATCGACGAGGCCCAGTTTGTGGACGTAGCCT CCTGGGCCTTCATAGAAAAGCTCATCCGGTCCATGCCCATCTTCATTGTTATGTCCCTGTGTCCCTTCCC TGAAACTCCCTGCGCAGCTGCCAATGCCATAATGAAGAACCGGAACACCACCTACATCACACTCGGTACC ATGCAGCCTCAGGAAATCCGGGACAAGGTCTGTGTTGACCTGAGTGTAAGCAGCATCCCCAGAGAGCTTG ACTCGTACCTGGTGGAGGGGAGCTGCGGGATTCCGTATTACTGTGAGGAACTGCTGAAAAACCTCGACCA CCACAGAATTCTCATTTTCCAACAAGCAGAGGCTGAGGAAAAGACAAACGTGACCTGGAATAACCTGTTC AAGTACTCTGTTAAGCCGACAGAAGACATGTATCTGTATACTTCCATAGCCGCGGGACAGAAAGAAGCCT GTTACCTTACAAGTGGTGTCAGACTAAAGAACTTGTCACCTCCAGCATCGCTCAAAGAAATCTCTCTGGT TCAACTGGACAGCATGAGCCTTTCCCATCAGATGCTGGTGAGGTGTGCTGCTATCATTGGTCTAACCTTC ACCACAGAGCTGCTGTTTGAGATTCTCCCCTGCTGGAACATGAAGATGATGATCAAGGCCCTGGCCACCC TAGTGGAATCAAATGTCTTTGATTGCTTTCGGAGTAGCAAAGACCTTCAACTAGCCTTAAAGCAAAACGT GACCACGTTTGAAGTTCATTATCGCTCTTTGTCCCTGAAGTCCAAGGAAGGGTTAGCTTACAGTGAGGAG GAGCAGCTCCGTGAAATGGAAGGAGAGGTGATTGAATGCCGCATCCTTCGGTTCTGCAGACCCATAATGC AGAAGACAGCCTACGAACTGTGGCTCAAGGACCAGAAGAAAGTCTTGCATCTGAAATGCGCCCGCTTTTT GGAGGAGAGTGCCCATCGGTGCAACCACTGCAGAAACAGAGACTTCATTCCTTACCACCACTTCATAGCG GACATTCGACTCAACACTCTGGACATGGATACTGTCAAGAAGATGGTGAAGTCCCACGGATTTAAAACTG AAGACGAGGTCATCTTTTCTAAATCAGAGATCCCCAGGAAATTCAAATTCCCCGAGAACATCAGCATCAC AGAAACAAGGGAAAAAATCTTGCATTTCTTTGACAATGTTATCATAAAGATGAGGACGTCTCAGGATGAT GTCATCCCTCTAGAATCGTGCCATTGTGAGGAGCTGCTCCAGATTGTCATCTTGCCTCTGGCCCAGCATT TCGTAGCCTTAGAAGAAAACAACAAAGCCTTGTACTACTTCCTAGAACTTGCATCTGCCTATCTCATCCT GGGAGACAACTATAACGCATACATGTATTTGGGCGAAGGGGAAAGGCTGTTGAAATCTCTGACAAATGAA GATTCTTGGAGTCAGACCTTTGAATATGCTACGTTTTATAGTCTCAAAGGTGAGATCTGTTTTAATATGG GACAGATGGTGCTCGCCAAGAAAATGCTGAGAAAAGCACTGAAGCTTCTCAACAGAATGTTTCCCTGCAA TCTACTCTCCCTGACTTTCCAAATGCACATTGAGAAAAACAGACTCTCCCACTTCATGAACCAGCATACC CAGGAGGGCTCGCTGCCAGGGAAGAAGCTGGCCCAACTTTTCCTGCAGTCGTCCTGCTTCTCCCTGCTGT GGAAGATCTATAGCTTGAACTTCTTTTTCCACTACAAGTACTATGGTCGTCTGGCAGCAATAATGCAGAT GAACACCTCGTTAGAAACTCAAAACAATTTCCAGATCATCAAGGCTTTCCTGGACTTTTCCCTGTACCGC CATCTGGCTGGATACGAGGGCGTGTGGTTCAAATATGAAATCCTGGTCATGGAGCAGCTCTTGAACCTCC CCCTGAAAGGCGAAGCCTTTGAAATCATGGCCTATGCAGCCGACGCACTGGGCCATATCAAGTTCTTAAC CGGTCATCTGGACTTGGCCATTGAATTAGGCTCCCGAGCTCACAAGATGTGGTCACTTCTCCGGAATCCC AACAAATACCATATGGTTCTCTGCAGACTGAGTAAACCTCTTTTCTTGAAGAGCAGATACAAGCATTTGG TCCAGGTGCTGGGATGGTTGTGGGACCTTTCTGTAACAGAGGAGCACATCTTCAGCAAGGCATTTTTCTA TTTCGTCTGCTTGGACATCATGCTTTATTCTGGCTTCATTTACAGAACATTTGAAGAATGTTTGGAATTC ATACACCACAATGAAGACAACAGAATCCTCAAGTTCCAAAGCGGACTCCTCCTGGGACTTTACTCCTGCA TAGCTGTCTGGTACGCCAGACTTCAGGAATGGGACAACTTTTACAAATTTTCCAATAGAGCGAAGACTTT AGTGACTCGAAGAACCCCAACGGTCCTTTACTACGAAGGAATTTCTAGGTATATGGAAGGGCAAGTCCTC CATCTTCAGAAGCAAATAGAAGAGCAGGCCGAGAATGCTCAGGACAGTGGGGTGGAGCTACTTAAGGCCT TAGAGACCCTTGTGGCTCAAAATACCACTGGCCCCGTCTTCTACCCCAGGCTCTACCATTTGATGGCCTA TGTCTGTATACTGATGGGAGACGGGCACAGTTGTGACTTCTTCCTAAACACAGCCTTGGAGCTCTCTGAG ACACAGGGGAATTTGCTGGAGAAATGTTGGCTGAGCATGAGTAAGGAATGGTGGTACTCAGCCCCCGAGT TGACAGGAGATCAATGGCTTCAGACAGTCTTGAGTCTCCCATCGTGGGATAAAATTGTATCAGGCAACGT AACCCTTCAGGATGTTCAAAAGAACAAATTCTTGATGAGAGTTAATATTCTGGACAATCCTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213767 representing NM_021684 Red=Cloning site Green=Tags(s) MSARRQELQDRAIVKIAAHLPDLIVYGDFSPERPSVKCFDGVLMFVDISGFTAMTEKFSTAMYMDRGAEQ LVEILNYYISAIVEKVLIFGGDILKFAGDALLALWKVERKQLKNIITVVIKCSLEIHGLFEAKEVEEGLD IRVKIGLAAGHITMLVFGDETRNYFLVIGQAVDDVRLAQNMAQMNDVILSPNCWQLCDRSMIEIERIPDQ RAVKVSFLKPPPTFNFDEFFAKCMAFMDYYPSGDHKNFLRLACMLESDPELELSLQKYVMEIILKQIDDK QLRGYLSELRPVTIVFVNLMFKEQDKAEVIGSAIQAACVHITSVLKVFRGQINKVFMFDKGCSFLCVFGF PGEKAPDEITHALESAVDIFDFCSQVHKIRTVSIGVASGIVFCGIVGHTVRHEYTVIGQKVNIAARMMMY YPGIVTCDSVTYDGSNLPAYFFKELPKKVMKGVADPGPVYQCLGLNEKVMFGMAYLICNRYEGYPLLGRV REIDYFMSTMKDFLMTNCSRVLMYEGLPGYGKSQVLMEIEYLASQHENHRAVAIALTKISFHQNFYTIQI LMANVLGLDTCKHYKERQTNLQNRVKTLLDDKYHCLLNDIFHVQFPVSREMSRMSKIRKQKQLEALFMKI LEQTVREERIIFIIDEAQFVDVASWAFIEKLIRSMPIFIVMSLCPFPETPCAAANAIMKNRNTTYITLGT MQPQEIRDKVCVDLSVSSIPRELDSYLVEGSCGIPYYCEELLKNLDHHRILIFQQAEAEEKTNVTWNNLF KYSVKPTEDMYLYTSIAAGQKEACYLTSGVRLKNLSPPASLKEISLVQLDSMSLSHQMLVRCAAIIGLTF TTELLFEILPCWNMKMMIKALATLVESNVFDCFRSSKDLQLALKQNVTTFEVHYRSLSLKSKEGLAYSEE EQLREMEGEVIECRILRFCRPIMQKTAYELWLKDQKKVLHLKCARFLEESAHRCNHCRNRDFIPYHHFIA DIRLNTLDMDTVKKMVKSHGFKTEDEVIFSKSEIPRKFKFPENISITETREKILHFFDNVIIKMRTSQDD VIPLESCHCEELLQIVILPLAQHFVALEENNKALYYFLELASAYLILGDNYNAYMYLGEGERLLKSLTNE DSWSQTFEYATFYSLKGEICFNMGQMVLAKKMLRKALKLLNRMFPCNLLSLTFQMHIEKNRLSHFMNQHT QEGSLPGKKLAQLFLQSSCFSLLWKIYSLNFFFHYKYYGRLAAIMQMNTSLETQNNFQIIKAFLDFSLYR HLAGYEGVWFKYEILVMEQLLNLPLKGEAFEIMAYAADALGHIKFLTGHLDLAIELGSRAHKMWSLLRNP NKYHMVLCRLSKPLFLKSRYKHLVQVLGWLWDLSVTEEHIFSKAFFYFVCLDIMLYSGFIYRTFEECLEF IHHNEDNRILKFQSGLLLGLYSCIAVWYARLQEWDNFYKFSNRAKTLVTRRTPTVLYYEGISRYMEGQVL HLQKQIEEQAENAQDSGVELLKALETLVAQNTTGPVFYPRLYHLMAYVCILMGDGHSCDFFLNTALELSE TQGNLLEKCWLSMSKEWWYSAPELTGDQWLQTVLSLPSWDKIVSGNVTLQDVQKNKFLMRVNILDNPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021684
ORF Size	4824 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021684.1, NP_067716.1
RefSeq Size	5177 bp
RefSeq ORF	4827 bp
Locus ID	59320
UniProt ID	Q9Z286
MW	185.9 kDa
Gene Summary	cytosolic adenylyl cyclase [RGD, Feb 2006]

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