Adcy10 (NM_021684) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sac
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213767 representing NM_021684 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCCCGAAGGCAGGAATTACAGGACAGGGCAATCGTCAAGATAGCTGCTCACTTACCGGACCTCA TTGTCTATGGAGATTTCTCTCCCGAGCGGCCGTCAGTGAAATGTTTTGATGGAGTTCTGATGTTTGTCGA TATTTCAGGCTTTACTGCAATGACTGAGAAGTTCAGCACAGCCATGTACATGGACCGAGGAGCCGAGCAG CTGGTGGAGATCCTCAACTACTACATAAGTGCGATAGTGGAGAAAGTACTGATTTTTGGAGGAGACATCC TAAAATTTGCAGGTGACGCCTTGTTGGCCCTGTGGAAAGTGGAACGAAAGCAACTGAAGAATATCATCAC GGTGGTAATTAAATGCAGCCTGGAGATTCATGGCTTGTTTGAAGCCAAGGAGGTTGAAGAAGGCCTGGAT ATTCGAGTTAAGATAGGACTGGCTGCTGGCCACATCACCATGTTGGTCTTTGGGGATGAAACACGGAACT ACTTCCTGGTGATTGGCCAAGCGGTGGATGATGTACGCCTTGCTCAGAACATGGCTCAGATGAATGATGT TATTCTGTCACCAAACTGCTGGCAGCTCTGTGATCGGAGCATGATTGAAATCGAGAGGATTCCGGATCAG AGAGCAGTTAAGGTTAGCTTCTTAAAACCACCCCCAACTTTTAACTTCGACGAGTTTTTTGCCAAGTGTA TGGCCTTCATGGATTATTATCCTTCTGGTGACCACAAAAACTTCCTAAGGCTTGCCTGCATGCTGGAGTC TGATCCTGAACTCGAGTTGTCTCTACAAAAGTATGTGATGGAAATCATTTTGAAGCAGATTGATGACAAG CAGCTTCGGGGCTATTTATCTGAGCTTCGTCCTGTGACGATCGTGTTTGTGAACTTGATGTTTAAAGAGC AAGACAAAGCAGAAGTCATAGGATCAGCCATCCAAGCTGCCTGTGTGCACATCACTTCCGTCTTGAAGGT CTTCCGAGGCCAGATCAATAAGGTCTTCATGTTTGATAAGGGCTGCTCCTTCCTCTGTGTCTTCGGTTTC CCTGGGGAAAAGGCCCCTGACGAGATCACTCACGCTTTGGAAAGTGCCGTGGATATATTCGACTTCTGCT CTCAGGTCCACAAAATCCGTACTGTCTCCATCGGCGTCGCCAGTGGGATTGTCTTCTGTGGGATCGTTGG ACACACTGTGAGACACGAGTACACAGTCATTGGCCAAAAGGTCAATATTGCTGCCAGGATGATGATGTAT TACCCAGGCATCGTGACCTGCGACTCTGTCACATACGATGGCAGCAACCTGCCAGCCTACTTTTTTAAAG AGCTTCCAAAGAAAGTCATGAAAGGAGTTGCGGATCCCGGACCAGTGTATCAGTGTCTGGGCCTCAATGA GAAAGTCATGTTTGGTATGGCCTATCTCATCTGCAACAGATATGAGGGCTACCCTTTGCTGGGTCGTGTT AGGGAGATCGACTATTTCATGTCTACTATGAAGGACTTTCTGATGACGAACTGCAGCCGAGTTCTAATGT ATGAAGGATTGCCAGGATATGGGAAAAGCCAGGTACTTATGGAAATCGAGTATCTGGCCTCCCAGCATGA GAACCATAGGGCTGTTGCTATTGCACTGACTAAGATCAGCTTCCATCAAAATTTTTACACTATCCAGATA CTCATGGCTAACGTACTAGGTCTGGATACTTGTAAACATTACAAAGAACGACAGACCAATCTTCAAAATA GAGTCAAGACGCTGTTGGATGATAAATACCACTGTCTCCTTAACGACATCTTCCATGTTCAGTTCCCCGT TTCCCGGGAGATGTCCAGGATGAGCAAGATAAGAAAGCAGAAGCAACTGGAAGCTCTGTTTATGAAGATC CTGGAGCAAACAGTGAGGGAAGAAAGGATTATCTTCATCATCGACGAGGCCCAGTTTGTGGACGTAGCCT CCTGGGCCTTCATAGAAAAGCTCATCCGGTCCATGCCCATCTTCATTGTTATGTCCCTGTGTCCCTTCCC TGAAACTCCCTGCGCAGCTGCCAATGCCATAATGAAGAACCGGAACACCACCTACATCACACTCGGTACC ATGCAGCCTCAGGAAATCCGGGACAAGGTCTGTGTTGACCTGAGTGTAAGCAGCATCCCCAGAGAGCTTG ACTCGTACCTGGTGGAGGGGAGCTGCGGGATTCCGTATTACTGTGAGGAACTGCTGAAAAACCTCGACCA CCACAGAATTCTCATTTTCCAACAAGCAGAGGCTGAGGAAAAGACAAACGTGACCTGGAATAACCTGTTC AAGTACTCTGTTAAGCCGACAGAAGACATGTATCTGTATACTTCCATAGCCGCGGGACAGAAAGAAGCCT GTTACCTTACAAGTGGTGTCAGACTAAAGAACTTGTCACCTCCAGCATCGCTCAAAGAAATCTCTCTGGT TCAACTGGACAGCATGAGCCTTTCCCATCAGATGCTGGTGAGGTGTGCTGCTATCATTGGTCTAACCTTC ACCACAGAGCTGCTGTTTGAGATTCTCCCCTGCTGGAACATGAAGATGATGATCAAGGCCCTGGCCACCC TAGTGGAATCAAATGTCTTTGATTGCTTTCGGAGTAGCAAAGACCTTCAACTAGCCTTAAAGCAAAACGT GACCACGTTTGAAGTTCATTATCGCTCTTTGTCCCTGAAGTCCAAGGAAGGGTTAGCTTACAGTGAGGAG GAGCAGCTCCGTGAAATGGAAGGAGAGGTGATTGAATGCCGCATCCTTCGGTTCTGCAGACCCATAATGC AGAAGACAGCCTACGAACTGTGGCTCAAGGACCAGAAGAAAGTCTTGCATCTGAAATGCGCCCGCTTTTT GGAGGAGAGTGCCCATCGGTGCAACCACTGCAGAAACAGAGACTTCATTCCTTACCACCACTTCATAGCG GACATTCGACTCAACACTCTGGACATGGATACTGTCAAGAAGATGGTGAAGTCCCACGGATTTAAAACTG AAGACGAGGTCATCTTTTCTAAATCAGAGATCCCCAGGAAATTCAAATTCCCCGAGAACATCAGCATCAC AGAAACAAGGGAAAAAATCTTGCATTTCTTTGACAATGTTATCATAAAGATGAGGACGTCTCAGGATGAT GTCATCCCTCTAGAATCGTGCCATTGTGAGGAGCTGCTCCAGATTGTCATCTTGCCTCTGGCCCAGCATT TCGTAGCCTTAGAAGAAAACAACAAAGCCTTGTACTACTTCCTAGAACTTGCATCTGCCTATCTCATCCT GGGAGACAACTATAACGCATACATGTATTTGGGCGAAGGGGAAAGGCTGTTGAAATCTCTGACAAATGAA GATTCTTGGAGTCAGACCTTTGAATATGCTACGTTTTATAGTCTCAAAGGTGAGATCTGTTTTAATATGG GACAGATGGTGCTCGCCAAGAAAATGCTGAGAAAAGCACTGAAGCTTCTCAACAGAATGTTTCCCTGCAA TCTACTCTCCCTGACTTTCCAAATGCACATTGAGAAAAACAGACTCTCCCACTTCATGAACCAGCATACC CAGGAGGGCTCGCTGCCAGGGAAGAAGCTGGCCCAACTTTTCCTGCAGTCGTCCTGCTTCTCCCTGCTGT GGAAGATCTATAGCTTGAACTTCTTTTTCCACTACAAGTACTATGGTCGTCTGGCAGCAATAATGCAGAT GAACACCTCGTTAGAAACTCAAAACAATTTCCAGATCATCAAGGCTTTCCTGGACTTTTCCCTGTACCGC CATCTGGCTGGATACGAGGGCGTGTGGTTCAAATATGAAATCCTGGTCATGGAGCAGCTCTTGAACCTCC CCCTGAAAGGCGAAGCCTTTGAAATCATGGCCTATGCAGCCGACGCACTGGGCCATATCAAGTTCTTAAC CGGTCATCTGGACTTGGCCATTGAATTAGGCTCCCGAGCTCACAAGATGTGGTCACTTCTCCGGAATCCC AACAAATACCATATGGTTCTCTGCAGACTGAGTAAACCTCTTTTCTTGAAGAGCAGATACAAGCATTTGG TCCAGGTGCTGGGATGGTTGTGGGACCTTTCTGTAACAGAGGAGCACATCTTCAGCAAGGCATTTTTCTA TTTCGTCTGCTTGGACATCATGCTTTATTCTGGCTTCATTTACAGAACATTTGAAGAATGTTTGGAATTC ATACACCACAATGAAGACAACAGAATCCTCAAGTTCCAAAGCGGACTCCTCCTGGGACTTTACTCCTGCA TAGCTGTCTGGTACGCCAGACTTCAGGAATGGGACAACTTTTACAAATTTTCCAATAGAGCGAAGACTTT AGTGACTCGAAGAACCCCAACGGTCCTTTACTACGAAGGAATTTCTAGGTATATGGAAGGGCAAGTCCTC CATCTTCAGAAGCAAATAGAAGAGCAGGCCGAGAATGCTCAGGACAGTGGGGTGGAGCTACTTAAGGCCT TAGAGACCCTTGTGGCTCAAAATACCACTGGCCCCGTCTTCTACCCCAGGCTCTACCATTTGATGGCCTA TGTCTGTATACTGATGGGAGACGGGCACAGTTGTGACTTCTTCCTAAACACAGCCTTGGAGCTCTCTGAG ACACAGGGGAATTTGCTGGAGAAATGTTGGCTGAGCATGAGTAAGGAATGGTGGTACTCAGCCCCCGAGT TGACAGGAGATCAATGGCTTCAGACAGTCTTGAGTCTCCCATCGTGGGATAAAATTGTATCAGGCAACGT AACCCTTCAGGATGTTCAAAAGAACAAATTCTTGATGAGAGTTAATATTCTGGACAATCCTTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_021684
Insert Size	4827 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021684.1, NP_067716.1
RefSeq Size	5177 bp
RefSeq ORF	4827 bp
Locus ID	59320
UniProt ID	Q9Z286
Gene Summary	cytosolic adenylyl cyclase [RGD, Feb 2006]

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