Dhx30 (NM_001013249) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MGC109411
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213688 representing NM_001013249 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCACCCTGGACTCATTCAGAAAAGATCGGACCCAGCACAGGCAGCGTCAATGCAAGCTTCCCCCAC CCCGCCTTCCACCCATGTGTGTCAACCCTGCCCCTGGAGGGACCATCTCTCGAGCTTCTAGGGACCTATT AAAAGAGTTTCCACAGCCTAAAAACCTTCTCAACAGCGTGATTGGAAGAGCTCTTGGCATCTCACATGCA AAAGACAAGCTAGTCTATGTGCACACGAATGGACCGAAGAAAAAGAAAGTCACACTGCACATAAAGTGGC CCAAGAGCGTGGAGGTGGAAGGCTATGGCAGCAAGAAGATCGATGCCGAGCGGCAGGCGGCGGCGGCAGC CTGCCAACTCTTCAAGGGCTGGGGTCTCCTGGGTCCACGGAATGAGCTGTTTGATGCAGCCAAATATCGA GTGCTAGCTGATCGTTTTGGATCTCCAGCTGACAGCTGGTGGCGCCCAGAACCCACCATGCCTCCAACTT CCTGGCGGCAGCTGAATCCTGAGAACATACGGCCAGCGGGTACTGGAGGACTGTCCCGATCCTTAGGCCG AGAGGAAGAGGAGGATGAGGAGGAAGAGCTAGAAGAGGGGACCATTGATGTGACGGAGTTTTTGTCCATG ACCCAGCAAGACTCCCACAACCCACTCAGGGACTCAAGGGGGGGCTCCTTTGAAATGACAGATGATGACA GTGCTATTAGAGCTCTGACCCAGTTTCCACTTCCCAAGAACCTTCTGGCCAAAGTGATTCAGATTGCAAC ATCTTCCTCCACAGCTAAGAACCTCATGCAGTTCCATACTGTGGGTACCAAGACCAAGCTGGCTACACTC ACTCTGCTCTGGCCCTGTCCCATGACCTTTGTCGCCAAAGGGCGCCGCAAAGCTGAGGCTGAGAATAAGG CAGCAGCCTTGGCTTGCAAGAAACTGAAGAGCCTGGGCCTTGTGGACAGAAACAATGAGCCACTTACCCA TGCCATGTACAACCTGGCTTCCCTGCGTGAGTTGGGTGAGACCCAGCGCCGGCCATGTACCATCCAGGTG CCTGAGCCCATCCTTCGCAAGATAGAGGCCTTCCTGAGTCATTACCCGGTGGACAGTTCATGGATTTCCC CAGAACTCCGGCTGCAGAGTGATGACATCTTGCCCTTAGGCAAGGACTCAGGGCCCTTGAGTGACCCTAT CACAGGCAAGCCATACATGCCCCTGTCAGAAGCAGAGGAGGTGCGCCTGAGCCAGAGCCTGCTAGAGCTG TGGCGGAGGAGAGGGCCAATCTGGCAGGAGGCCCCCCAGCTACCTGTGGACCCTCATCGGGACACTATCC TCAGTGCCATTGAACAACACCCAGTGGTAGTCATCTCTGGGGACACAGGCTGTGGGAAGACCACGCGTAT CCCTCAGCTGCTATTGGAGCGCTATGTGACTGAGGGTCGAGGTGCCCGCTGCAATGTGATCATCACACAA CCTCGCCGAATCTCAGCTGTGTCTGTGGCCCAGCGGGTCAGCCATGAACTGGGACCCTCCTTGCGCCGGA ATGTGGGCTTCCAGGTACGCTTGGAAAGCAAGCCTCCAGCCCGAGGTGGGGCGCTGCTCTTCTGCACTGT GGGTATCCTGCTTCGAAAGCTGCAGAGCAACCCCAGCCTGGAGGGTGTGAGCCACGTCATTGTGGATGAG GTCCATGAGCGGGATGTGAACACAGACTTCCTGCTGATTCTGCTCAAGGGCCTGCAGCGGCTCAACCCAG CCCTTCGGCTGGTGCTCATGAGTGCTACAGGAGATAATGAGCGCTTTTCCCGGTACTTTGGGGGCTGCCC TGTCATCAAGGTACCTGGCTTCATGTATCCTGTCAAGGAACACTACCTAGAGGACATCTTGGCCAAGCTG GGTAAACACCAGTACCCACACCGGCACCGGCACCATGAGTCTGAAGATGAGTGTGCACTTGATTTGGACC TTGTGACTGATCTGGTTCTGCACATCGATGCCCGTGGGGAACCAGGTGGGATCCTGTGCTTTCTGCCTGG TTGGCAAGAAATCAAAGGAGTGCAACAGCGGCTGCAGGAAGCCCTGGGCATGCATGAGAGCAAGTACCTT ATCCTACCAGTGCACTCCAATATCCCCATGATGGACCAGAAGGCCATATTCCAACAGCCTCCACTTGGGG TGCGCAAGATTGTATTGGCCACCAACATTGCTGAAACCTCCATCACAGTTAACGACATCGTGCATGTCGT GGACAGCGGTCTGCACAAAGAAGAACGCTATGACCTGAAGACCAAGGTGTCCTGCCTGGAGACCGTGTGG GTGTCGAGAGCAAATGTGATTCAGCGCCGGGGCAGGGCAGGCCGCTGCCAGTCAGGTTTTGCCTACCACT TGTTCCCGAGGAGCCGGCTGGAGAAAATGGTTCCTTTCCAAGTGCCAGAGATCCTGCGCACACCTCTTGA GAACCTGGTGCTGCAAGCCAAAATCCACATGCCTGAGAAGACGGCAGTGGAGTTCCTCTCTAAGGCTGTG GACAGTCCAAATATCAAGGCAGTGGATGAGGCCGTGATACTGCTCCAGGAGATTGGGGTGCTGGACCAGC GGGAGTACCTGACTACCTTGGGGCAGCGCCTGGCCCACATCTCTACTGATCCCCGACTGGCCAAGGCCAT AGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCC TTCAGCAGCAGTTTGCAGAACCGGGCAGAGGTAGACAAGGTGAAGGCATTGCTGAGCCATGACAGTGGCA GTGACCATTTGGCCTTCGTGCGGGCTGTGGCTGGCTGGGAGGAGGTACTGCGCTGGCAGGACCGTACCTC CAGGGAAAACTACCTGGAAGAAAACCTTCTGTATGCCCCCAGCTTGCGCTTCATCCACGGGCTCATCAAG CAGTTCTCAGAGAATATTTACGAGGCCTTCCTAGTGGGAAAGCCCTCTGACTGCACACTGCCCTCTGCTC AGTGCAATGAGTACAGCGAGGAAGAGGAGCTGGTGAAGGGTGTGCTGATGGCTGGCCTCTACCCCAACCT CATCCAGGTGAGGCAAGGTAAGGTTACTCGGCAAGGCAAGTTCAAACCCAACAGTGTCACTTACAGGACC AAATCTGGCAACATCTTGCTGCACAAGTCAACCATTAACAGGGAGGCCACCCGGTTACGGAGCCGATGGC TGACATATTTCATGGCCGTCAAGTCCAATGGTAGCGTCTTTGTTCGAGATTCTTCCCAGGTGCACCCACT AGCTGTGTTGCTCCTAACAGATGGGGACGTGCACATTCGAGATGACGGACGTCGGGCCACCATTTCACTG AGTGACAGTGACCTGCTGCGGCTGGAAGGTGATTCACGAACTGTGCGGTTGCTAAGGGAGCTTCGGCGGG CCCTAGGAAGGATGGTGGAGCGGAGCCTCCGCAGCGAGCTGGCTGCACTTCCTCTTAGTGTGCAGCAAGA ACACGGGCAGCTGCTTGCACTGCTGGCAGAGCTACTGCGGGGACCTTGTGGCAGCTTTGACGTGCGCAAG ACAGCTGATGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213688 representing NM_001013249 Red=Cloning site Green=Tags(s) MFTLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHA KDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYR VLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPAGTGGLSRSLGREEEEDEEEELEEGTIDVTEFLSM TQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATL TLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQV PEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLEL WRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQ PRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDE VHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKL GKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYL ILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVW VSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAV DSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDP FSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIK QFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRT KSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISL SDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDVRK TADD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001013249
ORF Size	3582 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001013249.1, NP_001013267.1
RefSeq Size	3853 bp
RefSeq ORF	3585 bp
Locus ID	367172
UniProt ID	Q5BJS0
MW	134 kDa
Gene Summary	RNA-dependent helicase (By similarity). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Associates with mitochondrial DNA (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (PubMed:21204022). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (By similarity).[UniProtKB/Swiss-Prot Function]

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