Dhx30 (NM_001013249) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MGC109411
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213688 representing NM_001013249 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCACCCTGGACTCATTCAGAAAAGATCGGACCCAGCACAGGCAGCGTCAATGCAAGCTTCCCCCAC CCCGCCTTCCACCCATGTGTGTCAACCCTGCCCCTGGAGGGACCATCTCTCGAGCTTCTAGGGACCTATT AAAAGAGTTTCCACAGCCTAAAAACCTTCTCAACAGCGTGATTGGAAGAGCTCTTGGCATCTCACATGCA AAAGACAAGCTAGTCTATGTGCACACGAATGGACCGAAGAAAAAGAAAGTCACACTGCACATAAAGTGGC CCAAGAGCGTGGAGGTGGAAGGCTATGGCAGCAAGAAGATCGATGCCGAGCGGCAGGCGGCGGCGGCAGC CTGCCAACTCTTCAAGGGCTGGGGTCTCCTGGGTCCACGGAATGAGCTGTTTGATGCAGCCAAATATCGA GTGCTAGCTGATCGTTTTGGATCTCCAGCTGACAGCTGGTGGCGCCCAGAACCCACCATGCCTCCAACTT CCTGGCGGCAGCTGAATCCTGAGAACATACGGCCAGCGGGTACTGGAGGACTGTCCCGATCCTTAGGCCG AGAGGAAGAGGAGGATGAGGAGGAAGAGCTAGAAGAGGGGACCATTGATGTGACGGAGTTTTTGTCCATG ACCCAGCAAGACTCCCACAACCCACTCAGGGACTCAAGGGGGGGCTCCTTTGAAATGACAGATGATGACA GTGCTATTAGAGCTCTGACCCAGTTTCCACTTCCCAAGAACCTTCTGGCCAAAGTGATTCAGATTGCAAC ATCTTCCTCCACAGCTAAGAACCTCATGCAGTTCCATACTGTGGGTACCAAGACCAAGCTGGCTACACTC ACTCTGCTCTGGCCCTGTCCCATGACCTTTGTCGCCAAAGGGCGCCGCAAAGCTGAGGCTGAGAATAAGG CAGCAGCCTTGGCTTGCAAGAAACTGAAGAGCCTGGGCCTTGTGGACAGAAACAATGAGCCACTTACCCA TGCCATGTACAACCTGGCTTCCCTGCGTGAGTTGGGTGAGACCCAGCGCCGGCCATGTACCATCCAGGTG CCTGAGCCCATCCTTCGCAAGATAGAGGCCTTCCTGAGTCATTACCCGGTGGACAGTTCATGGATTTCCC CAGAACTCCGGCTGCAGAGTGATGACATCTTGCCCTTAGGCAAGGACTCAGGGCCCTTGAGTGACCCTAT CACAGGCAAGCCATACATGCCCCTGTCAGAAGCAGAGGAGGTGCGCCTGAGCCAGAGCCTGCTAGAGCTG TGGCGGAGGAGAGGGCCAATCTGGCAGGAGGCCCCCCAGCTACCTGTGGACCCTCATCGGGACACTATCC TCAGTGCCATTGAACAACACCCAGTGGTAGTCATCTCTGGGGACACAGGCTGTGGGAAGACCACGCGTAT CCCTCAGCTGCTATTGGAGCGCTATGTGACTGAGGGTCGAGGTGCCCGCTGCAATGTGATCATCACACAA CCTCGCCGAATCTCAGCTGTGTCTGTGGCCCAGCGGGTCAGCCATGAACTGGGACCCTCCTTGCGCCGGA ATGTGGGCTTCCAGGTACGCTTGGAAAGCAAGCCTCCAGCCCGAGGTGGGGCGCTGCTCTTCTGCACTGT GGGTATCCTGCTTCGAAAGCTGCAGAGCAACCCCAGCCTGGAGGGTGTGAGCCACGTCATTGTGGATGAG GTCCATGAGCGGGATGTGAACACAGACTTCCTGCTGATTCTGCTCAAGGGCCTGCAGCGGCTCAACCCAG CCCTTCGGCTGGTGCTCATGAGTGCTACAGGAGATAATGAGCGCTTTTCCCGGTACTTTGGGGGCTGCCC TGTCATCAAGGTACCTGGCTTCATGTATCCTGTCAAGGAACACTACCTAGAGGACATCTTGGCCAAGCTG GGTAAACACCAGTACCCACACCGGCACCGGCACCATGAGTCTGAAGATGAGTGTGCACTTGATTTGGACC TTGTGACTGATCTGGTTCTGCACATCGATGCCCGTGGGGAACCAGGTGGGATCCTGTGCTTTCTGCCTGG TTGGCAAGAAATCAAAGGAGTGCAACAGCGGCTGCAGGAAGCCCTGGGCATGCATGAGAGCAAGTACCTT ATCCTACCAGTGCACTCCAATATCCCCATGATGGACCAGAAGGCCATATTCCAACAGCCTCCACTTGGGG TGCGCAAGATTGTATTGGCCACCAACATTGCTGAAACCTCCATCACAGTTAACGACATCGTGCATGTCGT GGACAGCGGTCTGCACAAAGAAGAACGCTATGACCTGAAGACCAAGGTGTCCTGCCTGGAGACCGTGTGG GTGTCGAGAGCAAATGTGATTCAGCGCCGGGGCAGGGCAGGCCGCTGCCAGTCAGGTTTTGCCTACCACT TGTTCCCGAGGAGCCGGCTGGAGAAAATGGTTCCTTTCCAAGTGCCAGAGATCCTGCGCACACCTCTTGA GAACCTGGTGCTGCAAGCCAAAATCCACATGCCTGAGAAGACGGCAGTGGAGTTCCTCTCTAAGGCTGTG GACAGTCCAAATATCAAGGCAGTGGATGAGGCCGTGATACTGCTCCAGGAGATTGGGGTGCTGGACCAGC GGGAGTACCTGACTACCTTGGGGCAGCGCCTGGCCCACATCTCTACTGATCCCCGACTGGCCAAGGCCAT AGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCC TTCAGCAGCAGTTTGCAGAACCGGGCAGAGGTAGACAAGGTGAAGGCATTGCTGAGCCATGACAGTGGCA GTGACCATTTGGCCTTCGTGCGGGCTGTGGCTGGCTGGGAGGAGGTACTGCGCTGGCAGGACCGTACCTC CAGGGAAAACTACCTGGAAGAAAACCTTCTGTATGCCCCCAGCTTGCGCTTCATCCACGGGCTCATCAAG CAGTTCTCAGAGAATATTTACGAGGCCTTCCTAGTGGGAAAGCCCTCTGACTGCACACTGCCCTCTGCTC AGTGCAATGAGTACAGCGAGGAAGAGGAGCTGGTGAAGGGTGTGCTGATGGCTGGCCTCTACCCCAACCT CATCCAGGTGAGGCAAGGTAAGGTTACTCGGCAAGGCAAGTTCAAACCCAACAGTGTCACTTACAGGACC AAATCTGGCAACATCTTGCTGCACAAGTCAACCATTAACAGGGAGGCCACCCGGTTACGGAGCCGATGGC TGACATATTTCATGGCCGTCAAGTCCAATGGTAGCGTCTTTGTTCGAGATTCTTCCCAGGTGCACCCACT AGCTGTGTTGCTCCTAACAGATGGGGACGTGCACATTCGAGATGACGGACGTCGGGCCACCATTTCACTG AGTGACAGTGACCTGCTGCGGCTGGAAGGTGATTCACGAACTGTGCGGTTGCTAAGGGAGCTTCGGCGGG CCCTAGGAAGGATGGTGGAGCGGAGCCTCCGCAGCGAGCTGGCTGCACTTCCTCTTAGTGTGCAGCAAGA ACACGGGCAGCTGCTTGCACTGCTGGCAGAGCTACTGCGGGGACCTTGTGGCAGCTTTGACGTGCGCAAG ACAGCTGATGACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001013249
Insert Size	3585 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001013249.1, NP_001013267.1
RefSeq Size	3853 bp
RefSeq ORF	3585 bp
Locus ID	367172
UniProt ID	Q5BJS0
Gene Summary	RNA-dependent helicase (By similarity). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Associates with mitochondrial DNA (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (PubMed:21204022). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (By similarity).[UniProtKB/Swiss-Prot Function]

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