Aqr (NM_001100987) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213548 representing NM_001100987 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTCCTGCGCAGCCCAAGAAAATCGTGGCCCCCACCGTGTCCCAGATCAACGCAGAGTTCGTCA CCCAGCTGGCATGTAAATACTGGGCTCCTCATATCAAGAAGAAATCACCTTTTGATATAAAAGTGATAGA AGAAATATATGAAAAAGAGATCGTCAAATCACGGTTTGCCATCAGAAAAATAATGCTGCTGGAATTTAGC CAATATCTTGAAAATTATCTTTGGATGAATTATTCGCCAGAAGTTTCCAGCAAAGCCTATTTAATGTCCA TCTGCTGTATGGTGAATGAGAAGTTCAGAGAAAATGTCCCAGCCTGGGAGACCTTTAAGAAGAAGCCAGA CCACTTCCCCTTCTTCTTTAAACGCATCTTGAAAGCAGCACTAGCTGAGACTGATGGCGAGTTCTCCCTC CACGAGCAGACCGTGCTCCTGCTTTTCCTGGATCATTGCTTCAATAGCTTGGAAGTAGACTTGATAAGGA GTCAAGTACAGCAGCTTATCTCCCTTCCTATGTGGATGGGCTTGCAGCCGGCACGATTGGAATTAGAATT AAAAAAGACACCAAAGCTAAGGAAATTCTGGAACTTGATTAAAAAGAATGATGAAAAGATGGACCCAGAA GCAAGAGAGCAGGCCTACCAAGAAAGGAGGTTTCTTTCCCAGCTCATCCAGAAGTTCATCTCCGTGTTGA AGTCCATCCCACTTTCTGAGCCTGTCACTATGGACAAAGTTCATTACTGTGAAAGATTCATCGAACTTAT GATTGATCTAGAGGCTCTGCTCCCCACAAGGCGCTGGTTCAACACCATCCTGGATGACTCCCACCTCTTG GTGCACTGTTACCTTTCCAGTCTTGTCCACAGGGAAGAGGATGGCCATCTGTTTTCCCAGCTATTAGACA TGCTGAAGTTCTATACGGGCTTTGAAATTAACGACCAGACTGGAAATGCCCTGACAGAGAACGAGATGAC CACGATTCACTATGACAGAATCACATCTCTGCAGAGAGCTGCGTTCGCGCATTTCCCTGAGCTCTATGAC TTTGCCCTCTCCAATGTGGCAGAAGTAGATGCCCGGGATTCCTTGGTCAAGTTTTTTGGGCCTCTCAGTT CAAACACACTCCACCAGGTGGCATCATACCTCTGCCTCTTGCCAACGCTCCCTAAAAATGAAGATACAAC TTTTGATAAAGAGTTTCTTCTAGAATTGCTGGTGTCTCGCCATGAACGCCGCATCTCTCAGATTCAGCAG TTGAATCAGATGCCTTTGTATCCTACTGAGAAAATTATCTGGGATGAAAACATCGTCCCTACCGAGTACT ATTCTGGGGAAGGCTGTCTTGCTCTCCCCAAGTTGAATTTGCAGTTTTTAACTCTACATGACTATCTCCT GAGGAACTTTAATCTCTTCCGCTTAGAATCAACTTATGAAATTAGACAAGACATTGAGGACAGCGTCAGC AGAATGAAGCCATGGCAATCTGAGTATGGCGGTGTCGTGTTTGGTGGTTGGGCACGAATGGCTCAGCCCA TTGTGGCTTTCACTGTAGTCGAGGTTGCCAAACCCAACATTGGTGAAAACTGGCCAACCCGAGTTCGTGC AGATGTCACCATCAATCTGAATGTCAGAGACCACATCAAGGATGAGTGGGAAGGTCTTCGCAAACACGAT GTATGCTTTTTAATTACTGTGCGTCCCACAAAACCTTACGGCACTAAGTTTGACAGGAGGAGGCCTTTTA TTGAGCAAGTTGGTCTGGTGTACGTCAGAGGCTGTGAGATCCAGGGCATGCTGGACGACAAAGGGCGAGT CATCGAAGACGGACCTGAACCCAGACCCAATCTTAGAGGAGAGTCAAGGACATTTCGAGTATTTTTGGAT CCAAACCAGTATCAACAGGATATGACCAATACTATACAAAATGGAGCCGAAGATGTGTACGACACTTTCA ATGTCATAATGAGGAGGAAACCAAAGGAGAATAACTTCAAGGCCGTGTTGGAGACTATTCGAAATCTGAT GAATACCGACTGCGTGGTCCCAGACTGGCTGCATGACATCATTTTAGGTTATGGGGACCCAAGTAGTGCC CATTATTCCAAAATGCCCAATCAGATTGCTACGTTGGATTTCAATGACACTTTTCTCTCCATTGAACATT TGAAAGCCAGTTTTCCTGGTCATAATGTCAAAGTGACCGTGAATGACCCAGCTCTACAAATACCCCCTTT CAGAATAACTTTCCCAGTAAGAAGTGGAAAGGGGAAGAAAAGAAAAGATGCAGATGGGGAAGAAGACGAC ACGGAAGAAGCAAAGACCTTAATTGTGGAACCTCACGTTATTCCTAATAGAGGTCCTTATCCTTACAACC AGCCCAAACGTAATACAATCCAGTTCACTCATACACAGATAGAGGCCATCCGGGCCGGAATGCAGCCTGG ACTGACTATGGTTGTGGGACCACCTGGTACAGGAAAAACAGATGTTGCAGTCCAGATCATTTCCAACATC TACCACAATTTTCCAGAACAGAGGACTTTAATTGTTACTCATTCCAATCAGGCTCTGAACCAGTTGTTTG AGAAAATCATGGCTTTAGACATTGATGAGCGCCATCTCCTGCGTCTGGGTCATGGAGAGGAAGAGCTAGA GACGGAGAAAGACTTTAGCAGGTATGGAAGAGTTAATTATGTCCTGGCTCGAAGAATAGAACTTTTAGAA GAAGTCAAACGATTACAAAAGAGTCTGGGGGTTCCGGGAGATGCTTCGTATACCTGTGAAACTGCAGGCT ATTTCTTCTTATATCAGGTAATGTCGCGTTGGGAAGAATACATGAGCAGAGTGAAGAACACAGGGAGCGC CAGCCCAGATGCTGCGGAGGTCGCCACGTTCTTCCCCTTCCATGAGTATTTTGCCAATGCTCCTCAGCCC ATCTTTAAAGGCAGATCTTATGAAGAAGACATGGAAATCGCAGAAGGGTGCTTCAGGCATATTAAGAAAA TCTTCACTCAGCTTGAGGAATTCAGAGCTTCAGAACTGCTTCGCAGTGGACTGGACAGGTCTAAATACCT CTTGGTGAAAGAAGCCAAAATCATTGCTATGACGTGTACTCATGCTGCCTTGAAACGACATGACTTGGTC AAGTTGGGTTTCAAGTATGACAACATTTTAATGGAAGAGGCTGCTCAGATTCTGGAGATAGAAACCTTTA TCCCACTACTTCTGCAGAATCCTCAAGACGGATTCAGCCGATTGAAACGATGGATTATGATTGGTGATCA CCACCAATTGCCTCCAGTCATTAAGAACATGGCCTTTCAGAAGTATTCCAATATGGAGCAGTCTCTTTTT ACTCGCTTTGTCCGGGTTGGAGTTCCTACTGTGGACCTTGATGCTCAAGGGAGAGCCCGGGCCAGCTTGT GTAACCTCTACAACTGGCGTTACAAAAACCTAGGGAACTTGCCACACGTGCAGCTCCTGCCAGAGTTCAG CACAGCCAACGCAGGCCTGCTGTACGACTTCCAGCTCATCAATGTGGAAGACTTCCAGGGAGTGGGAGAG TCGGAGCCTAACCCTTACTTCTACCAGAACCTCGGAGAGGCAGAGTACGTAGTAGCGCTCTTCATGTACA TGTGCTTGCTTGGCTACCCTGCGGACAAGATCAGCATTCTGACGACCTACAATGGACAGAAGCACCTGAT CCGGGACATCATCAATAGGCGCTGTGGGAATAATCCACTCATTGGGAGGCCGAACAAGGTGACAACTGTT GATAGGTTCCAAGGTCAACAGAATGACTACATCCTCCTTTCTCTAGTGCGGACCAGGGCCGTGGGCCATT TGAGGGATGTGCGCCGCTTGGTGGTAGCCATGTCGAGAGCCAGGCTTGGCCTGTATATCTTCGCTAGAGT ATCCCTCTTCCAAAATTGCTTTGAACTGACTCCTGCTTTCAGCCAGCTCACTGCCCGCCCACTGCATTTG CATATTATTCCAACAGAACCTTTCCCAACCTCCAGAAAGAATGGAGAGAGACCTCCTCATGAAGTACAGG TAATAAAGAACATGCCCCAGATGGCGAATTTTGTGTACAATATGTACATGCATTTGATACAGACGACCCA CCATTATCATCAGACTTTCTTACAACTACCGCCTGCCATGGTGGAAGAAGGTGAGGAGGTTCAGAGCCAA GAAACAGAAATGGAAGCAGAAGAGGAAACTGGGTCTGTTCAAGCTAACCTCACACCCAGTCCAACGGATG CCAGCCTGAGTCAAGAGACCCCAACTTCTCAGCCTGACTGCTCCAATCAGACGGAGGCTGCCTCCAGTCA CACAGAAGATACCTCTGCTCTGTGTGACATTACTACTGCAGCCGAGCCTGTCTCTGCAGCGGCAGAAGCT GCCACCCCACAAGATGGGCCTTCTGTTCCCGCAGAGACCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213548 representing NM_001100987 Red=Cloning site Green=Tags(s) MAAPAQPKKIVAPTVSQINAEFVTQLACKYWAPHIKKKSPFDIKVIEEIYEKEIVKSRFAIRKIMLLEFS QYLENYLWMNYSPEVSSKAYLMSICCMVNEKFRENVPAWETFKKKPDHFPFFFKRILKAALAETDGEFSL HEQTVLLLFLDHCFNSLEVDLIRSQVQQLISLPMWMGLQPARLELELKKTPKLRKFWNLIKKNDEKMDPE AREQAYQERRFLSQLIQKFISVLKSIPLSEPVTMDKVHYCERFIELMIDLEALLPTRRWFNTILDDSHLL VHCYLSSLVHREEDGHLFSQLLDMLKFYTGFEINDQTGNALTENEMTTIHYDRITSLQRAAFAHFPELYD FALSNVAEVDARDSLVKFFGPLSSNTLHQVASYLCLLPTLPKNEDTTFDKEFLLELLVSRHERRISQIQQ LNQMPLYPTEKIIWDENIVPTEYYSGEGCLALPKLNLQFLTLHDYLLRNFNLFRLESTYEIRQDIEDSVS RMKPWQSEYGGVVFGGWARMAQPIVAFTVVEVAKPNIGENWPTRVRADVTINLNVRDHIKDEWEGLRKHD VCFLITVRPTKPYGTKFDRRRPFIEQVGLVYVRGCEIQGMLDDKGRVIEDGPEPRPNLRGESRTFRVFLD PNQYQQDMTNTIQNGAEDVYDTFNVIMRRKPKENNFKAVLETIRNLMNTDCVVPDWLHDIILGYGDPSSA HYSKMPNQIATLDFNDTFLSIEHLKASFPGHNVKVTVNDPALQIPPFRITFPVRSGKGKKRKDADGEEDD TEEAKTLIVEPHVIPNRGPYPYNQPKRNTIQFTHTQIEAIRAGMQPGLTMVVGPPGTGKTDVAVQIISNI YHNFPEQRTLIVTHSNQALNQLFEKIMALDIDERHLLRLGHGEEELETEKDFSRYGRVNYVLARRIELLE EVKRLQKSLGVPGDASYTCETAGYFFLYQVMSRWEEYMSRVKNTGSASPDAAEVATFFPFHEYFANAPQP IFKGRSYEEDMEIAEGCFRHIKKIFTQLEEFRASELLRSGLDRSKYLLVKEAKIIAMTCTHAALKRHDLV KLGFKYDNILMEEAAQILEIETFIPLLLQNPQDGFSRLKRWIMIGDHHQLPPVIKNMAFQKYSNMEQSLF TRFVRVGVPTVDLDAQGRARASLCNLYNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGE SEPNPYFYQNLGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNKVTTV DRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFARVSLFQNCFELTPAFSQLTARPLHL HIIPTEPFPTSRKNGERPPHEVQVIKNMPQMANFVYNMYMHLIQTTHHYHQTFLQLPPAMVEEGEEVQSQ ETEMEAEEETGSVQANLTPSPTDASLSQETPTSQPDCSNQTEAASSHTEDTSALCDITTAAEPVSAAAEA ATPQDGPSVPAETK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100987
ORF Size	4452 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100987.1, NP_001094457.1
RefSeq Size	4888 bp
RefSeq ORF	4455 bp
Locus ID	366163
MW	170.8 kDa

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