Cacna1e (NM_019294) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CACHA1E; Cav2.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213444 representing NM_019294 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCGCTTCGGGGAGGCGGTGGTCGTTGGCAGACCAGGCTCAGGCGATGGAGACTCGGACCAGAGCA GGAACCGACAAGGAACCCCCGTACCGGCCTCGGGGCCGGCGGCCGCCTACAAGCAGTCAAAAGCGCAGAG GGCGACTATGGCTTTGTACAACCCCATACCAGTCCGGCAGAACTGTTTCACGGTCAACAGATCCCTGTTC ATCTTCGGAGAAGATAACATTGTCAGGAAATATGCCAAGAAACTCATCGATTGGCCGCCATTTGAGTACA TGATCCTGGCCACCATCATTGCCAACTGCATCGTCTTGGCCCTGGAGCAGCATCTTCCTGAGGATGACAA GACCCCAATGTCCCGAAGACTGGAGAAGACAGAACCATATTTTATTGGAATCTTCTGCTTTGAAGCTGGG ATCAAAATCGTGGCTCTAGGATTCATCTTCCATAAGGGTTCGTACCTCCGTAACGGCTGGAATGTCATGG ACTTCATTGTGGTCCTCAGTGGCATCCTGGCCACTGCAGGAACCCATTTCAACACCCACGTGGACCTGCG GACCCTCCGGGCTGTGCGTGTCCTGAGGCCCTTAAAGCTCGTGTCAGGAATACCTAGCCTGCAGATCGTG CTGAAGTCCATCATGAAGGCCATGGTGCCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATTCTCA TGTTTGCCATCATTGGCTTGGAGTTCTACAGTGGCAAGTTGCATCGAGCATGCTTCATGAACAATTCAGG TATTCTAGAGGGATTTGATCCTCCTCACCCGTGTGGTGTACAGGGCTGCCCGGCTGGTTATGAATGTAAG GACTGGATCGGCCCCAATGACGGGATCACCCAGTTTGACAACATCCTTTTTGCTGTGCTGACTGTCTTCC AATGCATCACTATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTGGAA CTGGCTGTACTTCATCCCCCTCATCATCATCGGATCCTTCTTTGTTCTCAACCTTGTCCTGGGCGTGCTT TCCGGGGAATTTGCCAAAGAAAGAGAGAGAGTGGAGAACCGAAGAGCATTCATGAAGCTCCGGCGTCAGC AACAGATTGAGCGCGAGTTGAATGGCTACCGCGCCTGGATAGACAAAGCAGAGGAAGTCATGCTTGCTGA AGAAAATAAAAACTCGGGAACATCAGCCTTGGAAGTGCTTCGAAGGGCAACCATCAAAAGGAGCCGGACG GAGGCCATGACCCGAGACTCCAGCGATGAGCACTGCGTTGATATCTCTTCAGTGGGCACACCTCTTGCCA GAGCCAGTATCAAGAGCACAAAAGTAGACGGAGCCTCCTATTTCCGGCACAAGGAACGGCTTCTGCGTAT CTCTATCCGACACATGGTCAAGTCGCAAGTGTTCTACTGGATCGTCCTGAGTGTTGTGGCTCTCAATACT GCCTGTGTGGCCATCGTTCATCACAACCAGCCACAGTGGCTCACTCACCTCCTCTACTATGCAGAATTTT TGTTTCTGGGACTCTTCCTGTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCACGCCTTTATTTTCA CTCATCCTTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGCATCTTTGAGGTCGTTTGGGCAATCTTC CGACCTGGCACATCTTTTGGAATCAGTGTCTTACGAGCTCTCCGGCTCCTAAGGATATTTAAAATAACCA AGTATTGGGCATCCCTCCGGAATCTGGTTGTTTCCTTGATGAGCTCCATGAAGTCTATCATCAGCTTACT CTTCCTCCTCTTCCTCTTCATTGTTGTCTTTGCTCTCCTGGGAATGCAGCTCTTTGGAGGCAGGTTTAAC TTTAATGATGGGACTCCTTCGGCTAATTTTGATACCTTCCCTGCAGCTATCATGACAGTGTTCCAGATCC TGACGGGGGAAGACTGGAATGAAGTGCTGTATAACGGCATCCGCTCCCAGGGTGGGGTGAGCTCAGGCAT GTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGAAACTACACACTGCTGAATGTATTCTTGGCT ATCGCTGTGGACAATCTCGCCAATGCCCAGGAACTGACCAAGGATGAGCAGGAGGAAGAAGAAGCCTTCA ACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCTCCCAACATGCCTTCCATTGA GAGGGAGAGGAGACGCCGACACCACATGTCTGTGTGGGAACAGCGCACCAGCCAGCTGAGGAGGCACATG CAAATGTCTAGCCAAGAAGCCCTCAACAAAGAGGAGGCCCCACCAATGAACCCCCTCAATCCACTCAACC CACTGAGTCCTCTCAACCCACTCAATGCTCATCCTAGCCTCTATCGGAGGCCCAGGCCCATTGAGGGCCT ACCCCTGGGCTTGGGTCTTGAAAAATGTGAAGAGGAGCGTATCAGCCGTGGAGGTTCTCTCAAGGGGGAC ATAGGTGGCCTGACTAGCGTCCTGGACAACCAGAGGAGCCCTTTGTCCCTGGGCAAACGGGAGCCACCGT GGCTGCCCAGATCCTGTCATGGAAACTGTGACCCAACCCAGCAGGAGACTGGGGGAGGAGAGACTGTGGT GACTTTTGAGGACCGGGCCAGGCACAGGCAGAGTCAGAGGCGAAGCCGGCATCGCCGAGTCAGGACTGAG GGCAAGGAGTCTGCCTCTGCCTCCAGAAGCAGGTCTGCCAGCCAGGAGCGGAGTCTGGATGAAGGGGTGT CCATCGATGGGGAGAAGGAACACGAGCCTCAGAGCAGCCACAGGAGCAAGGAGCCAACCATCCATGAAGA AGAGAGAACTCAGGACTTAAGGAGGACCAACAGTCTGATGGTCCCCAGGGGCTCAGGACTGGTAGGAGCT CTTGATGAGGCAGAAACACCTCTAGTCCAGCCCCAACCTGAGCTGGAAGTGGGGAAGGATGCAGCTCTGA CAGAGCAGGAGGCTGAAGGCAGCAGCGAGCAAGCCCTGCTTGCGGATGTACAGCTGGATGTGGGCCGGGG CATTAGCCAGAGTGAACCTGACCTCTCCTGCATGACAACCAACATGGACAAGGCTACCACTGAGAGCACC AGTGTCACAGTCGCCATCCCTGACGTGGACCCCTTGGTGGACTCAACAGTGGTGAACATTAGCAACAAGA CGGATGGAGAAGCCAGTCCCTTGAAGGAAGCAGAGACCAAAGAGGAAGAGGAAGAGGTGGAAAAGAAGAA GCAGAAGAAGGAGAAGCGGGAGACTGGAAAAGCCATGGTGCCCCATAGCTCTATGTTCATCTTCAGCACC ACTAACCCGATCCGCAAGGCTTGCCACTACATTGTGAATCTACGCTACTTTGAGATGTGCATTCTCTTGG TGATTGCGGCCAGCAGCATTGCCCTGGCAGCTGAGGACCCTGTACTAACTAACTCAGAGCGTAACAAAGT CCTGAGATATTTCGACTATGTTTTCACAGGAGTGTTTACCTTTGAGATGGTGATAAAGATGATAGACCAA GGCTTGATCCTGCAGGATGGATCGTACTTCCGAGACCTCTGGAATATCCTGGACTTTGTGGTAGTGGTTG GTGCATTGGTAGCATTTGCTCTGGCGAACGCTTTGGGAACCAACAAGGGGCGGGATATCAAGACGATCAA GTCTCTGAGGGTACTTCGAGTTCTGCGACCACTGAAAACCATCAAGCGCTTGCCCAAACTGAAGGCTGTG TTTGACTGCGTGGTGACCTCCTTGAAGAATGTCTTCAACATACTCATTGTCTACAAGCTCTTTATGTTCA TCTTTGCCGTCATTGCTGTTCAGTTATTCAAGGGAAAGTTCTTTTATTGCACCGACAGTTCCAAGGACAC AGAGAAGGAATGCATAGGCAACTATGTAGACCACGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAGTGG AAGCGTCATGAATTCCACTATGACAACATCATCTGGGCCCTGCTGACCCTCTTCACCGTCTCCACGGGGG AAGGATGGCCTCAGGTTCTGCAGCACTCGGTAGATGTGACAGAGGAGGACAGAGGCCCAAGCCGCAGCAA CCGCATGGAGATGTCCATCTTCTATGTGGTCTACTTTGTGGTCTTCCCTTTCTTCTTTGTCAATATCTTC GTGGCCCTCATTATCATCACCTTCCAGGAGCAAGGGGACAAGATGATGGAGGAGTGTAGCCTAGAAAAGA ATGAGCGGGCATGCATCGACTTCGCCATTAGTGCCAAACCTCTCACCCGCTACATGCCACAGAACAGGCA CACCTTCCAGTACCGCGTGTGGCACTTCGTAGTCTCGCCATCCTTTGAGTATACCATCATGGCCATGATT GCCTTGAACACCGTTGTGCTGATGATGAAGTACTATTCTGCTCCTTGGACCTATGAGCTCGCCCTAAAGT ACCTGAACATCGCCTTCACTATGGTGTTTTCCCTAGAATGTGTCCTAAAGGTCATCGCTTTCGGCTTCTT GAACTATTTCCGAGATACCTGGAACATCTTTGACTTCATTACCGTGATCGGCAGTATCACAGAAATTATC CTGACAGACAGCAAGCTGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGGAGCTCTTCCGAGCTGCAC GGCTCATAAAGCTTCTGCGACAGGGCTACACCATCCGCATCTTACTGTGGACCTTCGTGCAGTCCTTTAA GGCTCTCCCCTATGTCTGCCTTCTGATTGCCATGCTTTTCTTCATCTACGCTATCATTGGGATGCAGGTG TTTGGAAACATAAAGTTAGATGAGGAGAGTCATATCAACCGGCACAACAACTTTCGGAGTTTCTTCGGGT CTCTCATGCTGCTTTTCAGGAGCGCCACAGGGGAGGCTTGGCAGGAGATTATGTTGTCATGTCTGGGCGA GAAGGGCTGTGAGCCTGACACCACTGCACCCTCAGGGCAGAACGAGAGTGAGCGCTGTGGCACTGACCTG GCCTACGTTTATTTTGTCTCCTTCATCTTCTTCTGCTCCTTCCTGATGCTCAACCTGTTTGTGGCTGTTA TCATGGACAACTTTGAGTACCTGACTCGAGATTCGTCCATCCTGGGGCCTCACCACTTGGATGAATTTGT CCGTGTCTGGGCAGAGTATGACAGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTG ACTCTCATGTCACCACCGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCCTATAAGAGGTTGGTCC TGATGAATATGCCAGTGGCTGAGGACATGACAGTCCATTTCACCTCCACACTTATGGCTCTGATCCGGAC AGCTCTGGACATTAAAATCGCCAAAGGTGGTGCAGACAGACAGCAGCTAGACTCAGAGCTACAAAAAGAG ACCCTGGCCATTTGGCCTCACCTGTCCCAAAAGATGTTGGATCTGCTAGTGCCCATGCCCAAAGCCTCTG ACCTGACTGTGGGCAAGATCTATGCAGCAATGATGATTATGGACTACTATAAACAGAGTAAGGTAAAGAA ACAGAGACAGCAGCTGGAAGAACAGAAAAATGCACCCATGTTCCAGCGCATGGAGCCCTCCTCACTGCCT CAGGAGATCATTTCTAATGCCAAAGCCCTGCCTTATCTCCAGCAGGACCCTGTTTCTGGCCTGAGTGGTC GGAGTGGATACCCTTCAATGAGTCCACTCTCCCCTCAAGAAATATTCCAGTTGGCTTGTATGGACCCAGC GGATGATGGACAATTCCAGGAACAGCAATCGCTGGAGCCAGAGGTTAGTGAATTAAAAAGTGTGCAGTCC TCTAACCATGGCATCTACCTTCCTCCGGACACCCAGGAGCATGCGGGATCTGGGAGGGCATCCTCTATGC CACGTCTGACTATGGATCCCCAGGTGGTAACAGACCCTAGCTCTATGCGACGTTCATTTTCTACAATTCG GGATAAGCGTTCAAATTCCTCCTGGTTGGAGGAGTTCTCCATGGAACGTAGCAGTGAAAACACCTACAAG TCACGACGCAGAAGTTACCATTCCTCCCTGAGGCTGTCAGCCCACCGCCTGAATTCTGACTCTGGTCACA AGTCTGACACTCACCGCTCAGGAGGCAGGGAGAGAGGCCGATCCAAAGAACGAAAGCATCTCCTCTCTCC GGATGTCTCCCGCTGCAATTCTGAGGAGCGAGGAACCCAGGCTGACTGGGAGTCCCCAGAACGTCGCCAG TCCAGGTCACCCAGTGAGGGAAGGTCACAAACCCCCAACAGACAGGGTACAGGTTCACTGAGTGAGAGCT CCATTCCCTCTATCTCCGACACCAGCACCCCAAGACGAAGTCGTCGCCAGCTCCCACCTGTGCCACCAAA GCCTCGGCCCCTCCTCTCCTACAGCTCCCTGATGAGACACACTGGCGGCATCTCTCCACCTCCTGATGGA AGTGAGGGTGGATCCCCACTGGCCTCTCAAGCTCTGGAAAGCAACAGCGCTTGCCTAACCGAGTCTTCCA ACTCCTTGCACCCCCAGCAGGGCCAGCACCCTTCCCCACAGCACTACATCTCTGAGCCCTATCTGGCCCT CCATGAAGACTCCCACGCCTCAGACTGTGGCGAGGAGGAGACACTCACCTTTGAGGCAGCTGTAGCTACT AGCTTGGGCCGGTCCAATACCATTGGCTCTGCCCCACCCCTACGGCACAGCTGGCAGATGCCTAATGGGC ACTATCGGCGGCGGAGGCTGGGGGGCCTGGGCCTGGCCATGATGTGTGGAGCTGTCAGTGACCTCCTGAG TGACACGGAAGAAGACGATAAGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213444 representing NM_019294 Red=Cloning site Green=Tags(s) MARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRATMALYNPIPVRQNCFTVNRSLF IFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAG IKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIV LKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECK DWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVL SGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRT EAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNT ACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIF RPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFN FNDGTPSANFDTFPAAIMTVFQILTGEDWNEVLYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLA IAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHM QMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLPLGLGLEKCEEERISRGGSLKGD IGGLTSVLDNQRSPLSLGKREPPWLPRSCHGNCDPTQQETGGGETVVTFEDRARHRQSQRRSRHRRVRTE GKESASASRSRSASQERSLDEGVSIDGEKEHEPQSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGA LDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLADVQLDVGRGISQSEPDLSCMTTNMDKATTEST SVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKQKKEKRETGKAMVPHSSMFIFST TNPIRKACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQ GLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAV FDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREW KRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIF VALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMI ALNTVVLMMKYYSAPWTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEII LTDSKLVNTSGFNMSFLELFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQV FGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDL AYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEML TLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKE TLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLP QEIISNAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLEPEVSELKSVQS SNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYK SRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQ SRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDG SEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVAT SLGRSNTIGSAPPLRHSWQMPNGHYRRRRLGGLGLAMMCGAVSDLLSDTEEDDKC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019294
ORF Size	6885 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019294.2, NP_062167.2
RefSeq Size	6973 bp
RefSeq ORF	6888 bp
Locus ID	54234
MW	259.2 kDa
Gene Summary	T-type low voltage-activated calcium channel that may be involved in neuronal firing [RGD, Feb 2006]

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