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Ppp1r12a (NM_053890) Rat Tagged ORF Clone
CAT＃: RR213295
- TrueORF®
Ppp1r12a (Myc-DDK-tagged ORF) - Rat protein phosphatase 1, regulatory (inhibitor) subunit 12A (Ppp1r12a), (10 ug)
"NM_053890" in other vectors (3)
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CNY 12,540.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | M110; MBSP; Mypt1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213295 representing NM_053890
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAGCGCTGGATCGGCTCCGAGACGGACC TCGAGCCTCCCGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTCGCCGC CTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAAT GTGGACGGACTCACCGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAG AAAATGGAGCAAATATCAATCAACCTGACAATGAAGGCTGGATTCCACTCCATGCAGCCGCTTCCTGTGG ATATCTGGATATTGCAGAATTTTTGATTGGTCAAGGAGCACATGTAGGAGCTGTCAACAGTGAAGGTGAC ACACCTTTAGATATTGCAGAGGAGGAAGCAATGGAAGAGCTACTTCAAAATGAGGTTAATCGGCAAGGTG TTGATATAGAAGCAGCTCGAAAAGAAGAGGAACGCATAATGCTTAGAGACGCGAGGCAGTGGTTGAACAG TGGTCACATCAGTGACGTCCGGCATGCAAAGTCCGGAGGCACAGCACTCCACGTGGCAGCGGCCAAAGGG TATACAGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGATTATGATGGCTGGA CACCTCTTCATGCTGCAGCTCACTGGGGTAAAGAAGAAGCATGTCGGATTTTAGTGGACAATCTGTGTGA TATGGAGACGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTGGGATATCTA GAGGAGTTGCAAAAAAAACAAAATCTGCTCCATAGTGAAAAGCGGGATAAGAAATCTCCACTGATTGAAT CAACAGCAAATATGGAAAATAATCAACCACAGAAGACTTTTAAAAACAAGGAAACGTTGATTATTGAGCC AGAGAAAAATGCATCTCGAATCGAGTCTCTGGAGCAAGAAAAGGCTGATGAGGAGGAGGAAGGCAAGAAG GATGAGTCCAGCTGCTCCAGTGAGGAGGATGAGGAGGATGACTCCGAGTCCGAAGCGGAGACAGATAAGA CAAAACCCATGGCTTCTGTAACTAATGCTCACACTGCCAGCACTCAGGCAGCTCCTGCCGCTGTGACAAC ACCTACTCTGTCTTCCAACCAGGGGACCCCTACATCACCTGTTAAAAAGTTTCCTACATCAACTACAAAA ATTTCTCCCAAAGAAGAAGAAAGAAAAGATGAATCTCCTGCATCCTGGAGGTTAGGACTTAGAAAGACTG GCAGTTATGGTGCCCTGGCTGAGATCACTGCATCTAAAGAAGCTCAGAAGGAGAAAGACACTGCAGGCGT GATACGTTCAGCTTCGAGTCCCAGACTCTCGTCCTCTTTGGATAATAAAGAAAAGGAGAAAGACAATAAA GGAACAAGACTTGCATATGTCGCCCCTACAATCCCAAGGCGACTAGGCAGTACGTCTGACATTGAAGAGA AGGAAAACAGAGAGTCTTCAAATTTGCGAACAAGTAGTTCTTACACAAGAAGAAAATGGGAAGATGATCT TAAAAAAAATAGTTCAATCAATGAAGGATCTACTTACCATAGAAGTACCTCAAATCGTTTGTGGGCTGAG GATAGTACTGAGAAAGAGAAGGACAGTGCTCCTACCGCAGCGACCATTCTTGTTGCTCCAACTGTTGTAA GTGCTGCAGCTTCTTCTACCACAGCCCTGACCACAACTACTGCTGGCACTCTTTCCTCCACATCAGAGGT CAGGGAGAGACGCAGGTCATACCTCACTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAGGAAAGCAAGA TCTAGACAAGCAAGACAGTCTAGACGGTCAACACAGGGGGTGACACTGACTGACCTCCAGGAAGCCGAAA AGACAATAGGAAGAAGCCGTTCTACGAGAACCAGAGAACAAGAAAACGAAGAAAAAGACAAAGAAGAAAA GGAAAAGCAGGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAGTATCTAGAGAAGATGAATATAAG CAAAAGTATTCCAGAACATACGATGAGACTTATGCACGTTACAGACCAGTGTCAACTTCAAGTTCAAGCA CTCCGTCGTCCTCCTCACTTTCTACTCTAGGCAGTTCACTCTATGCCTCAAGTCAGCTCAACAGGCCAAA CAGCCTTGTAGGTATAACCTCTGCCTACTCCCGGGGATTAACCAAAGACAATGAAAGAGAGGGAGAGAAA AAAGAAGAGGAAAAAGAAGGGGAAGATAAGTCACAACCTAAATCAATCAGAGAACGACGGCGACCAAGAG AAAAACGGAGGTCTACTGGAGTCTCCTTCTGGACACAAGATAGTGATGAAAATGAGCAAGAGCGGCAGTC AGACACCGAGGATGGCTCCAGCAAGAGGGACACTCAGACGGATTCTGTTTCCAGGTATGACAGCAGTTCC ACGTCATCAAGCGATCGGTATGACTCCTTGCTGGGTCGTTCTGCCTCATACAGTTACTTAGAAGAAAGGA AACCATATGGTAGCCGACTAGAAAAGGATGACTCAACTGACTTCAAAAAGCTTTATGAACAAATCTTAGC TGAAAATGAAAAACTAAAGGCACAGCTACATGACACAAATATGGAACTAACGGATCTAAAGTTGCAGTTG GAAAAAGCTACCCAGAGACAAGAACGATTTGCTGACAGGTCACTATTGGAGATGGAAAAAAGGGAACGAA GAGCTCTAGAAAGAAGAATATCTGAGATGGAAGAGGAGCTCAAAATGTTACCAGACTTAAAAGCAGACAA CCAGAGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213295 representing NM_053890
Red=Cloning site Green=Tags(s) MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYAN VDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGD TPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKG YTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDILGYL EELQKKQNLLHSEKRDKKSPLIESTANMENNQPQKTFKNKETLIIEPEKNASRIESLEQEKADEEEEGKK DESSCSSEEDEEDDSESEAETDKTKPMASVTNAHTASTQAAPAAVTTPTLSSNQGTPTSPVKKFPTSTTK ISPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDTAGVIRSASSPRLSSSLDNKEKEKDNK GTRLAYVAPTIPRRLGSTSDIEEKENRESSNLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSTSNRLWAE DSTEKEKDSAPTAATILVAPTVVSAAASSTTALTTTTAGTLSSTSEVRERRRSYLTPVRDEESESQRKAR SRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKDKEEKEKQDKEKQEEKKESEVSREDEYK QKYSRTYDETYARYRPVSTSSSSTPSSSSLSTLGSSLYASSQLNRPNSLVGITSAYSRGLTKDNEREGEK KEEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQERQSDTEDGSSKRDTQTDSVSRYDSSS TSSSDRYDSLLGRSASYSYLEERKPYGSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQL EKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053890 |
| ORF Size | 2928 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053890.1, NP_446342.1 |
| RefSeq Size | 3300 bp |
| RefSeq ORF | 2931 bp |
| Locus ID | 116670 |
| UniProt ID | Q10728 |
| MW | 109.7 kDa |
| Gene Summary | selectively binds to PP-1C and increases the substrate specificity of the phosphatase towards myosin [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213295 | Ppp1r12a (untagged ORF) - Rat protein phosphatase 1, regulatory (inhibitor) subunit 12A (Ppp1r12a), (10 ug) |
CNY 12,540.00 |
|
| RR213295L3 | Lenti ORF clone of Ppp1r12a (Myc-DDK-tagged ORF) - Rat protein phosphatase 1, regulatory (inhibitor) subunit 12A (Ppp1r12a), (10 ug) |
CNY 14,250.00 |
|
| RR213295L4 | Lenti ORF clone of Ppp1r12a (mGFP-tagged ORF) - Rat protein phosphatase 1, regulatory (inhibitor) subunit 12A (Ppp1r12a), (10 ug) |
CNY 15,680.00 |
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