Sphkap (NM_001127492) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1311951; Skip
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213119 representing NM_001127492 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAACTCCCGGCTTTCTGTGCAAAGCCATGTGGGTAGTTGAACCAAACTCTGCTACCTGCCTTTCTG CTGCCCCGCCGGTCAAGAGACACTGCCAAGAATCCAGCAACGTGGAATCACCACTGATGCCCGAGGACTC GGAGCCACAGCAGATCACCAGCAGTGCAGCAGGCAGCCTGGCTGGCTCCATCACGGCCTGTAAGAAGGTT CTTCGAAGCAATAGCTTGCTGGAGTCCACAGACTACTGGTTGCAGAACCAGAGGACGCCTTGCCAGATTG GGTTTGTGGAAGACGAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAACCTGGATGTCAACAAGGATGG CTGCAGCACAGAGAACCTGCAGCAGAAACTGGTCAACGTTTCACCAGACCTTCCAAAACTCATCAACTCC ATGAATGTCCAGAAGCCAAAAGAAAATGAAATTGTCCTCCTAAGTGGGCTAGCCTCTGGAAACCGCCAGG CAGATTTTGATGTTTCACAGTGCCCTTGGCTACCAGATATCTGCTTGGTCCAGTGCGCAAGAGGGAACAG ACCAAACAGTACCAACTGCATCATCTTTGAGATCAACAAGTTCTTAATTGGTCTGGAAGTGGTGCAGGAG CGACAGCTCCACCTGGAGACCAGCGTGTTGAAGCTGGAGGATGATACAAACTGTTCCCTGTCTTCGATTG AAGAAGATTTCCTTACAGCCTCAGAGCATTTGGAGGAAGAAATCGAGGTGGAAGACTGCAAAAGTGGTTT GGAAACTATAAATGTGTCAGCTAACGTTCTGGAAAGCAAAAGGCCAAAGGAAGCTACCCAGGAAGGATGG GATTACCACAAGGAAATATTGCATTGTGCTTTAGGTGAAAAACACATCAGAAAACACCGTATGCCCTCAA TGAAAACAGAAAGGTCAAAGGAAAACACAGCAGAGAACACAGCCCTGCAAAGTCTCAATCCTTCAGCCAG ACCATCACGCTTGAAAAGTGAAGTGGCGGGGAGCAAGCAGCCAGCCACGAACTACTCTTACCCCGAAAAT ATCAGAGGAGAAATAGAAACATCTCAGATGCTGTTCATCCCCAGGGATGCCTATCTCTCCATGGTTTCTT CATGTGGTGTACTCACTGAGCAGGGAAGCAACCACAGAGACCATGACGCCACTCCAAACTCCCTTCCTCC CGTTCAAAATGGAGAAGCCACCGCGGGCGAGTACGCTACCAACTTAGCAGAATCTGTAATGCAAGATGCC TTCATCCGATTGTCTCAGTCGCAACCTACACTCCCCCAGGAATCTGCAGTCAGTTTCTCTGTGGGAAGCG CTCTGCTCCCCAGTGGTTGTTGCACCAAAGATATGGTGGTCCCTCGTTCATGGAATGAGCTTCCTAAAAT TGTCATCGTGCAGAGCCCAGAGGGCAGCGACACAGCCCCTGAGCCAAGTGTCTCCTCCTGGCCCGACCTG GAAGTGTCTGTCGAGACCCCGGGCATCTTTTCTGAAGAGAGCTCCAGCAGACCCACTCAGAGTGCCCTAG AAGTTGCTTTGGCTTGTGCAGCCACTGTGATTGGAACCACTTCCAGTCCACAGGCCACAGAAAGATTCAC CATGGAGCAAGAATCTCTGGTATCTACCTACACCCAGAGAGGCAATGGGATACAGCAAACTCCAGTGCCC AGAGTATTCATGGGACCTTCTACAACTGAATACTCCTTCCCATCAGCTCTGTGTGGCATGACCCAAGTGG CAAGCGCTGTTGCTGTCTGTGGCCTAGGGGAAAGAGAAGAGGTGACATATTCTGTAGCTCCAAGTGACCT CCTGCCCACACCTGGGGCTTCTGAAGAAAGATCCTCCATTGGAAGTTTAGTGACAGAGGAAAGCACAGAA CTGGGGAAGGAAGCCATTGCAGAGGCTCTGCTCAGGGAGGCTACTCTGGTTTTAGCGAGGCCGAATGCAT ACAGCAGCGTCGGAGAGCTCTTGGAATCTGTGAACCGGAGGATCATAGAAACCACTTCTAAAACCCAGAT GCTGTGCACAGAAAATGTTCGAAGGAATGAACTGGCACACACCTTGTCCAATGTCATCCTCAAGCATTCC ATTGATGAGCTGCACCAGAAAAATATCATGGCTCACCCCACCGAGGAAAGGCATCCCGGTGGGACTCTGA ACACCTTAATGGAAAGTGTGAACCAGTTGCTGCACAACGTGATGTGCTTCACCTTCAGAAAGATGAATCA CATCGTAACACTGGGTGAGCATCCCTCATTTGATAAGGCTGCTGGTCATGCATGGGTTAAAGCCTCGGCT TGCTCCAGCAGCCATCCCTCTAGCAATGCACACGGTACTGGCCTTGTCATCAGAGATCTTGTAGAAGATG CATCTCCTAAGCCAGACAAGGGAGGAGCAAGGCCAGAACTGGTCAACAACCCCAGGCTGCAATCTGAATT CTCATGCAGTCACAGAATGCTTGATTCAACTGCTAGGACATTCCCCAAGGAAATGTATCCAAAAGGGATT GTGGGAGAGGATACAAGAAACCCTCTTCATACACTAAGTTATGACAGCAGTGAACAAAGAACTTCTACAG ATATAGGAAAATTAACAACAGTAGGAGAGGTCCGTAGTACCTTCCAGGAATCTGAAGACAGCATTGTTCC AAAAGCCCAAGAGAAACACACTTGTGCCACAACTCTAAACAACGAAGCTCAAATTAACCTATCCTTGTTA GGGGATGACCTAGTGGTTCCTGATCAATCTACACTAGAGGCAAAGCAGTCCGAGGTCTATGGCATCACAA ACTTTGCGGAAGAATTGGCAGAGACTGTTGTCTCCATGGCAACTGAAATCGCAGCAATCTGCCTTGACAA CTCCCATGGAAAACAGCCGTGGTTTTGTGCTTGGAAGAGAGGGAACGAATTTCTGATGACGCCTAACGCA TCCTGCCGATCCTTGAAGAGGAAGAAGGAAAACTCGGGCACGGGAAGCACTGTGAGGAAACACAAGCCAC CTCGCCTCAGTGAGATCAAGAGAAAAGCAGATGAGCACCCAGAGCTGAAAGAGAAGCTGATGAACAGAGT CATGGATGAGTCCATGAACCTCGAAGACATTCCCGATTCCGTCAGTACTTTTGCAAATGAAGTGGCAGCC AAGATCATGAACCTAACAGAATTCTCCACGGTGGATGGAGTGTGGCAAACCCAGAGTTGTTCCCGGAATC GGCTTCTGGGTGGTGATAGGTGGAACCGGCTGAAGGCCTCGAGCTGTGAAAGTATTCCGGAGGAGGACTC TGAAGCCAGGGTCTTTGTAAACAGCCTGGGTTTAATGAGTACCTTAAGCCAACCCGTTAGCAGGGCCAGC TCAGTCTCCAAACAGTCCAGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAAACAGATGG AAAACGAAGGGAGGGGGTTCGAGTTGCTGCTGGATTACTACGCTGGCAAAAATGCCAGCAGTATCCTGAA CTCAGCAATCCAGCAGGCATGCCAGAAAAACGACCACCTCAACGTAAGACCAAGCTGCCCCTCTAAGCAG TCCAGCACAGAAAGCATAACAGAGGAGTTCTATAAGTACATGCTGAGAGACATTGCCAAAGAAAACAAAG ATGGCGCCCTGTCCAGACGAAGCAGCCATGATTGGAGCACGGGCTTGCTGTCTCCTTCCGCGCGATCCCC ACTGTGTTACAGGCAGTCATCTATGCCTGACAGCAGGTCCCCGTGCTCCAGGTTAACAGTAAATGCACCT ATCAAAGCCAACTCCTTAGATGGTTTTGCCCAAAACTGCCCTCAAGATTCTATAAATGTACAGCCAGTCA GTAGGGCTTCCTCGTCTGGCCTCTGCAAATCAGACTCCTGTTTATATCGCCGAAGTGGGACTGACCAGAT CACAAACATGCTAATTCATGAAACATGGGCCAGCTCCATTGAGGCTCTCATGAGAAAGAACAAAATCATT GCTGATGACGGCGAGGCAGCTAACGCTAGCCCTGGTCCTGTTTCTGGCGGCTCGCCTTCGCAAGTAGAGA AGTGTGCCAACAGACTAGTCACCGGCACAGGACACAAGGGACCAGCTCTACTTGTACAGGAGTCTGTTGA TTACCAGAGAAAGGATGCTGTCACTGAAGGCAATTGTTCCCCAGTGTCATCTCCAAGCAAAATGGCTCCT GTTAAAAAGCCCAGTGGTTTTGATCCTACAAGAGAAACCTCTGCATGCCACAATGCTGTTGCTCTCAAAA GCCCCAGGCGGTCTCTTTGCTCAAGGGAGGTGCCTTTGATTCAGATAGAGACCGATCAGAAAGAAGAATG CGTTGGAGAATCTGAAACCTTACTTCCCCAAAGTGGCTCCCTAGAGGAAGCAGAAGGGCCCCAGCCTGAG GAAACCATCCCAGATGTGGCCAGAAGCGAAGACACAGCCCTGAGCGCCTGTCAAAGCTCTCATGACAGCC TTGAGACCAGAGAAGAATTAGAAGTGGATGTCTTGAAAGAAGACATAACCCTAGATGAGTCCCGGAACCC TCCTAGCAGCAGCGAAGAGAGTACAGGCAGCTGGTCCCAGCTGGCCAATGAGGAGGACAACCCAGATGAC ACAAGTAGCTTTCTGCAGCTCAGTGAGCGGTCCATGAGCAATGGCAACACTAGTGGCACTAGCAGTCTTG GCATTATGGACCTGGACATTTATCAGGAAAGCATACCGTCTTCTCCCATGATTAATGAATTAGTAGAAGA AAAGGAGATTCTTAAAGAACAATCAGAAAGCGTAAAGGAACGTGCCTCTGGACTGCCAGGGAGAGCAGCC AGCCCCCAGAGGAGTCTACTGGTGATCAACTTTGACCTGGAGCCAGAGTGTCCTGATGCTGAGCTGCGAG CCACTCTGCAGTGGATAGCTGCCTCCGAGCTGGGCATCCCAACAATCTACTTTAAGAAATCTCAGGAAAG CAGAATTGAAAAGTTTCTAGATGTTGTGAAGCTTGTTCATCAGAAGTCCTGGAAGGTGGGAGATATATTT CATGCCGTTGTCCAGTACTGCAAAATGCATGCAGAGCAGAAAGAGGGAACTCCGAGTCTCTTTGACTGGC TCCTGGAACTAGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213119 representing NM_001127492 Red=Cloning site Green=Tags(s) MSTPGFLCKAMWVVEPNSATCLSAAPPVKRHCQESSNVESPLMPEDSEPQQITSSAAGSLAGSITACKKV LRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINS MNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQE RQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGW DYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPEN IRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDA FIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDL EVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVP RVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTE LGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHS IDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPSFDKAAGHAWVKASA CSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGI VGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLL GDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNA SCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAA KIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRAS SVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQ SSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAP IKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKII ADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAP VKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPE ETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDD TSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAA SPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIF HAVVQYCKMHAEQKEGTPSLFDWLLELG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001127492
ORF Size	5124 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001127492.1, NP_001120964.1
RefSeq Size	5423 bp
RefSeq ORF	5127 bp
Locus ID	316561
MW	187.1 kDa

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