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Arpc1a (NM_031146) Rat Tagged ORF Clone
CAT＃: RR212853
- TrueORF®
Arpc1a (Myc-DDK-tagged ORF) - Rat actin related protein 2/3 complex, subunit 1A (Arpc1a), (10 ug)
"NM_031146" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212853 representing NM_031146
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCATCAGTTTCTGCTAGAGCCAATCACCTGCCATGCCTGGAACAGGGATCGGACCCAGATCG CACTGAGCCCCAATAATCATGAAGTGCACATCTACAAGAAGAATGGGAGTCAGTGGACGAAAGCTCACGA GCTGAAGGAACATAACGGACACATCACAGGTATTGACTGGGCTCCTAAGAGCGACCGTATTGTCACTTGT GGGGCGGACCGCAACGCCTATGTCTGGAGTCAGAAAGATGGCATCTGGAAGCCGACCCTGGTGATCCTGA GGATTAACCGTGCAGCCACTTTCGTGAAGTGGTCTCCACTCGAGAACAAGTTTGCTGTGGGGAGTGGAGC CCGGCTCATCTCTGTCTGCTACTTTGAGTCTGAAAATGACTGGTGGGTCAGCAAGCACATTAAGAAGCCG ATCCGCTCCACAGTCCTCAGCCTGGACTGGCATCCCAACAACGTTTTGCTGGCTGCAGGCTCCTGTGACT TCAAATGCAGAGTATTTTCGGCCTACATCAAAGAGGTGGATGAGAAGCCAGCCAGCACGCCCTGGGGCAG CAAGATGCCTTTTGGTCAGCTGATGTCTGAGTTTGGTGGCAGTGGCACTGGTGGCTGGGTGCACGGGGTC AGCTTCTCTGCCAGTGGGAGCCGGCTGGCCTGGGTCAGCCACGACAGCACAGTGTCTGTCGCTGATGCCT CAAAAAGTGTGCAGGTTTCAACTCTGAGAACGGAGTTCCTGCCGCTGCTCAGTGTGTCATTTGTCTCAGA GAACAGCGTTGTGGCTGCCGGCCATGACTGCTGCCCGATGCTCTTTAACTACGATGACCGTGGCTGTTTG ACCTTTGTGTCCAAGCTAGACGTCCCAAAACAGAGCATCCAGCGCAACATGTCTGCTATGGAACGCTTCC GTAACATGGATAAGAGGGCCACTACCGAGGACCGCAACACAGCCTTGGAAACACTGCACCAGAACAGCAT CACTCAAGTGTCTATTTATGAAGTGGACAAGCAAGATTGTCGCAAATTTTGCACTACTGGCATCGATGGA GCCATGACAATCTGGGATTTCAAGACTCTAGAGTCTTCCATCCAGGGTCTCCGGATAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212853 representing NM_031146
Red=Cloning site Green=Tags(s) MSLHQFLLEPITCHAWNRDRTQIALSPNNHEVHIYKKNGSQWTKAHELKEHNGHITGIDWAPKSDRIVTC GADRNAYVWSQKDGIWKPTLVILRINRAATFVKWSPLENKFAVGSGARLISVCYFESENDWWVSKHIKKP IRSTVLSLDWHPNNVLLAAGSCDFKCRVFSAYIKEVDEKPASTPWGSKMPFGQLMSEFGGSGTGGWVHGV SFSASGSRLAWVSHDSTVSVADASKSVQVSTLRTEFLPLLSVSFVSENSVVAAGHDCCPMLFNYDDRGCL TFVSKLDVPKQSIQRNMSAMERFRNMDKRATTEDRNTALETLHQNSITQVSIYEVDKQDCRKFCTTGIDG AMTIWDFKTLESSIQGLRIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031146 |
| ORF Size | 1110 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031146.2, NP_112408.1 |
| RefSeq Size | 1627 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 81824 |
| UniProt ID | Q99PD4 |
| MW | 41.6 kDa |
| Gene Summary | Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212853 | Arpc1a (untagged ORF) - Rat actin related protein 2/3 complex, subunit 1A (Arpc1a), (10 ug) |
CNY 3,990.00 |
|
| RR212853L3 | Lenti ORF clone of Arpc1a (Myc-DDK-tagged ORF) - Rat actin related protein 2/3 complex, subunit 1A (Arpc1a), (10 ug) |
CNY 6,080.00 |
|
| RR212853L4 | Lenti ORF clone of Arpc1a (mGFP-tagged ORF) - Rat actin related protein 2/3 complex, subunit 1A (Arpc1a), (10 ug) |
CNY 6,650.00 |
