Eogt (NM_001009502) Rat Tagged ORF Clone
CAT#: RR212658
- TrueORF®
Eogt (Myc-DDK-tagged ORF) - Rat glycosyltransferase Aer61 (Aer61), (10 ug)
"NM_001009502" in other vectors (3)
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CNY 6,750.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Aer61 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR212658 representing NM_001009502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAATGCTGCTTGTCTTTGGAGTACTGCTTCATGAAGTCCCCCTGAGTGGCCAGGACGAGGCTCATC CTGAGGCTGACAGAGTGCCCGGCGAAGCCCTCTATGACTACTCCAGCCTCCGCCTCCCAGAGGAGCACAT TCCCTTCTTCCTGCACAGTAACAGGCATGTTGCCTCTGTGTGCAGGGAGGATTCCCACTGTCCGTATAAG AAACACTTAGAGAGTCTAAATTCCTGCTGGGGGTATGAGAAGTCCTGCACACCAGAGTCTAGATTTGGCT CCCCCATTTGCAGCTATGTGGACTTGGGATGGACCGACACCCTGGAGTCAGCCCAGGACATGTTCTGGAA ACAAGCTGACTTCGGATATGCTCGGGAGCGGCTGGAGGAGATACGAATGTTCTGCCGGCCAGAGAGTGCG AGCGACTCAAGTCTGCTTTGCTCTCGTTACCTTCAGTATTGTAGAGCTACTGGTCTATACCTGGACTTAA GAAACATCAAGAGAAACCATGACAGATTCAAGGAAGATTTTCTTCAGGGTGGTGATATTGGAGGATACTG TAAACTTGACCGTCATGCACTGGTGTCTGAAGGTCAGCGCAAAAGCCCTCTGCAGTCTTGGTTTGCTGAG CTACAAGGCTACACTCAGCTCAATTTCAGGCCTATAGAAGATGCTAAATGTGACATCGTTGTTGAAAAAC CAACATATTTTATGAAATTAGATGCAGGTATTAACATGTACCACCACTTTTGTGACTTCCTGAATCTCTA CCTCACTCAGCACATAAACAATTCTTTCAGTACAGACGTGTACATTGTCATGTGGGACACCAGCTCTTAT GGATACGGTGACCTCTTCTCTGATACCTGGAAAGCATTTACTGACTATGACGTCATACATTTGAAAACCT ACGATTCCAAAAAGGTGTGTTTTAAAGAAGCTGTGTTTTCGTTGCTGCCCCGCATGCGGTACGGGCTGTT CTATAACACGCCTCTGATCTCTGGCTGTCAGAACACTGGGTTATTCAGGGCCTTCTCCCAGCACGTGCTG CACAGGCTGAACATCTCTCAAGAGGGACCCAAGGATGGGAAACTTCGAGTTACCATTCTTGCACGCAGCA CAGAATACCGGAAAATCCTGAACCAAAATGAGCTGGTAAATGCCCTGAAAACAGTATCCACATTTGAAGT CCGGGTGGTTGATTACAAGTACAGAGAACTCGGGTTTTTAGATCAGCTAAGGATCACGCACAACACGGAC ATATTTATCGGCATGCACGGAGCTGGTCTTACCCACTTACTTTTCCTTCCAGACTGGGCTGCTGTATTCG AGCTGTACAACTGTGAGGATGAGCGCTGTTACTTAGACCTGGCCAGGCTCAGAGGCATCTACTACATCAC CTGGCAGAAGCCCAGCAAAGTGTTTCCTCAAGATAAGGGGCACCATCCAACTCTGGGGGAGCACCCGAAG TTTACCAACTACTCTTTCGACGTAGAGGAATTCATGTACCTTGTCCTGCAGGCTGCAGAACATGTACTGC AGCACCCGCAGTGGCCGTTGAAGAAGAACCACGATGAACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212658 representing NM_001009502
Red=Cloning site Green=Tags(s) MLMLLVFGVLLHEVPLSGQDEAHPEADRVPGEALYDYSSLRLPEEHIPFFLHSNRHVASVCREDSHCPYK KHLESLNSCWGYEKSCTPESRFGSPICSYVDLGWTDTLESAQDMFWKQADFGYARERLEEIRMFCRPESA SDSSLLCSRYLQYCRATGLYLDLRNIKRNHDRFKEDFLQGGDIGGYCKLDRHALVSEGQRKSPLQSWFAE LQGYTQLNFRPIEDAKCDIVVEKPTYFMKLDAGINMYHHFCDFLNLYLTQHINNSFSTDVYIVMWDTSSY GYGDLFSDTWKAFTDYDVIHLKTYDSKKVCFKEAVFSLLPRMRYGLFYNTPLISGCQNTGLFRAFSQHVL HRLNISQEGPKDGKLRVTILARSTEYRKILNQNELVNALKTVSTFEVRVVDYKYRELGFLDQLRITHNTD IFIGMHGAGLTHLLFLPDWAAVFELYNCEDERCYLDLARLRGIYYITWQKPSKVFPQDKGHHPTLGEHPK FTNYSFDVEEFMYLVLQAAEHVLQHPQWPLKKNHDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001009502 |
ORF Size | 1581 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001009502.1, NP_001009502.1 |
RefSeq Size | 2142 bp |
RefSeq ORF | 1584 bp |
Locus ID | 494219 |
UniProt ID | Q5NDL0 |
MW | 61.5 kDa |
Gene Summary | Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN212658 | Eogt (untagged ORF) - Rat glycosyltransferase Aer61 (Aer61), (10 ug) |
CNY 6,750.00 |
|
RR212658L3 | Lenti ORF clone of Eogt (Myc-DDK-tagged ORF) - Rat glycosyltransferase Aer61 (Aer61), (10 ug) |
CNY 8,740.00 |
|
RR212658L4 | Lenti ORF clone of Eogt (mGFP-tagged ORF) - Rat glycosyltransferase Aer61 (Aer61), (10 ug) |
CNY 9,500.00 |