Kif13b (NM_213626) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212134 representing NM_213626 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACTCCAAAGTGAAAGTGGCGGTACGGGTCCGGCCAATGAACCGACGAGAAATTGACTTACATA CCAAATGTGTGGTGGATGTTGAAGCAAACAAAGTTATTTTGAATCCTATAAATACAAATCTTTCCAAAGG AGATGCCCGGGGCCAGCCAAAGATATTTGCCTATGATCATTGTTTCTGGTCCATGGATGAGTCTGTCCGA GAGAAGTATGCAGGTCAAGATGATGTTTTCAAGTGCCTTGGGGAGAACATTCTTCAGAATGCATTTGATG GCTACAATGCCTGTATCTTTGCCTATGGGCAGACAGGGTCTGGAAAATCTTACACTATGATGGGAACAGC CGACCAACCGGGGTTAATCCCACGTCTCTGCAGTGGACTCTTTGAACGAACCCAGAAAGAAGAGAATGAA GAACAGAGTTTTAAAGTCGAAGTGTCGTACATGGAAATTTATAATGAAAAAGTCCGAGACCTTCTTGACC CCAAAGGAAGCCGACAAACGTTGAAAGTGAGAGAGCACAGTGTGTTGGGACCCTACGTGGATGGACTTTC TAAACTGGCTGTCACAAGCTACAAGGATATAGAGTCTCTGATGTCTGAGGGCAACAAGTCTCGGACAGTT GCTGCAACCAACATGAATGAAGAAAGTAGCCGTTCCCATGCAGTCTTCAAAATTACCCTGACACATACTC TGTATGATGTGAAGTCTGGGACATCTGGAGAGAAAGTGGGCAAGCTGAGCTTAGTGGATTTAGCTGGCAG TGAACGAGCCACGAAAACTGGAGCTGCGGGCGACAGGCTGAAGGAGGGCAGCAACATCAACAAGTCCCTC ACAACCCTGGGTCTGGTTATCTCTGCCCTAGCCGATCAGGGTGCTGGCAAAAACAAAAATAAATTTGTTC CCTATCGAGACTCAGTTCTCACATGGCTGCTCAAGGACAGTCTGGGAGGTAATAGCAAGACCGCCATGGT GGCCACTGTGAGCCCAGCTGCTGATAACTATGATGAAACACTTTCAACCCTGAGGTATGCAGATCGAGCA AAGCACATCATCAACCATGCTGTGGTGAATGAGGATCCTAATGCTCGGATTATCCGAGATCTCCGTGAAG AGGTGGAGAAGCTCCGTGAGCAGCTGACTAAGGCAGAGGCAATGAAATCTCCAGAGCTGAAAGATCGCCT GGAAGAATCTGAGAAACTCATCCAGGAAATGACTGTGACCTGGGAGGAGAAACTAAGGAAAACCGAGGAG ATTGCGCAGGAGCGGCAGAAGCAGCTTGAGAGCCTTGGCATATCTCTTCAGACTTCTGGAATCAAAGTTG GGGATGACAAATGCTTCCTGGTTAACTTGAATGCGGACCCAGCTCTGAATGAACTCCTGGTGTACTACTT AAAGGAGCACACGCTGATAGGGTCAGCCAATTCTCAGGACATCCAGCTCTGTGGGATGGGCATTCTCCCT GAGCATGGTATCATAGACATCATGCCCGAAGGCCAGGTCATGCTGACTCCTCAGAAGAATACCAGAACAT TTGTCAATGGGTCTTCTGTCTCCAGCCCTATCCAGCTACACCATGGGGACAGGATACTATGGGGAAACAA CCATTTCTTCAGACTCAATCTGCCAAAAAAGAAAAAGAAAGCAGAGCGAGAGGACGAGGAGCGTGAGGCA TCTCTGAAAAATGACAGCAGTTCTGAGCAGCTGGATGCCGACGGCGACTCCTCCAGTGAGGTGTCCAGCG AAATCAACTTCAACTTTGAGTATGCACAGATGGAGGTGACCATGAAGGCTCTGGGCAGCAATGACCCAAT GCAGTCCATATTGAGCAGCCTAGAACAGCAGCATGAGGAGGAGAAGCGGTCTGCTCTCGAGCGCCAGAGG CTCATGTACGAACACGAACTGGAACAGCTCCGAAGAAGGCTGTCTCCGGAGAGGCAGAATTGTCGAGGTG TGGACCGGCTTTCCTTCCATTCACCCAGTGCCCAGCAGCGCTTGAGACAGTGGGCTGAGGAGAGAGAAGC AACATTGAATAACAGCTTGATGAGGCTGAGGGAGCAGATTGTAAAGGCCAACCTGCTGGTACGGGAAGCT AGTTACATCGCAGAGGAGCTGGATAAGAGAACCGAGTATAAAGTTACCCTCCAGATTCCAGCCTCCAGTC TGGATGCCAATAGGAAGCGAGGCTCTCTCCTCAGTGAACCTGCCATCCAGGTGAGAAGAAAAGGGAAAGG GAAGCAGATTTGGTCTTTGGAAAAGCTGGAAAACAGGCTTTTGGATATGAGAGACCTCTACCAGGAGTGG AAAGAATGTGAAGAAGACAGCCCGGTTTCCCGATCCTACTTCAAGCGTGCTGACCCATTCTATGATGAGC AGGAAAATCATAGTCTCATCGGTGTGGCCAATGTCTTCCTGGAGACCCTGTTTTATGATGTGAAGTTACA ATATGCTGTGCCGATCATCAACCAGAAGGGAGAGGTGGCAGGCCGGCTGCATGTGGAGGTGATGCGGCTC AGTGGGGCCATTGGGGAGAGGATTGCAGGAGGAGACGATCCTACAGAGGTCAGCTCTGAGAAGGAAGTCC AGGAGAACAGGCTGGTCTGCATGGTTAAAATACTCCAGGCCACAGGGTTGCCTCAGCATCTGTGCCACTT TGTCTTCTGCAAATATGACTTCTGGGATCAGCAGGAGCCAGTGACCGTTGCTCCCGAAGTGGACACCTCT TCATCTCCCACCAGCAAGGAGCCACAGTGTATGGTTGTGTTCGATCATTGCAGCGAGTTCAGTGTTAACA TCACAGAAGACTTTATTGAATATCTCTCAGAAGGGGCCCTGGCCATTGAAGTGTATGGACATAAAATGAA TGATCCCCGGAAAAACCCAGCCCTGTGGGATTTGGGAATCATCCAAGCAAAAACAAGGAGTCTTCGGGAC AGGTGGAGTGAAGTCACCAGAAAGTTGGAATTCTGGGTTCAGATCTTGGAACAGAATGAGAATGGCGAAT ACTGCCCTGTGGAAGTAATTGCTGCAAAAGATGTCCCAACAGGGGGGATCTTCCAGCTCCGACAGGGGCA GTCTCGGCGAGTTCAAGTTGAAGTGAAGTCAGTGCAGGAGTCAGGGACTTTGCCACTGATGGAAGAGTGC ATATTGTCGGTTGGCATTGGCTGTGTGAAAGTTAGACCGCTCAGGTCCCCTAAGACCCACGAGAACATCC ATGAGGAAGAGGAGGACATGGACAGCTACCAGGATAGGGATTTAGAGCGGCTTCGTAGAAAATGGCTCAA TGCATTAACCAAACGTCAAGAATACTTGGATCAACAACTGCAGAAGCTTGTCAGTAAACATGACAAAACT GAGGATGATGCTGACCGAGAGGCACAGCTCTTGGAGATGCGGCTGACACTCACAGAGGAAAGAAATGCGG TGATGGTGCCGTCTGCTGGGAGTGGCATTCCGGGGGCACCGGCAGAATGGACCCCAGTGCCTGGGATGGA GGCCCACATTCCTGTTGTATTTCTTGACTTAAATGCGGATGATTTCAGCTCTCAGGATAACCTTGATGAC CCAGAGGCCGGATGGGATGCTACCCTGACTGGGGAAGAGGAGGAAGAGTTCTTTGAGTTGCAGATTGTGA AGCATCACGATGGAGAGGTGAAGGCAGAGGCCTCCTGGGACTCTGCAGTGCACAACTGTCCTCAGCTCAG TAAAGGCACACCTGCTGACGAGCGAGTGTTCCTCATCCTGAGAGTGGCAGTCCAGCTCAGCCACCCTGCA GACATGCAACTGGTCCTACGCAAGAGGATTTGTGTCCATGTTCATGGGCGCCAGGGCTTTGCTCAAAGTC TCCTCAAAAAGATGACTCATCGGAGCTCTATCCCTGGCTGTGGAGTGACCTTTGAAATTGTCTCCAATAT TCCAGAGGTGGATGCCCAGGGAGTGGAGGAGCGAGAGGCACTGGCAAGGATGGCTGCTAACGTGGAAAAC GCAGCTTCTGCTGACTCCGAGGCCTGTATTGAGAAGTACTTGAGGAGTGTGCTGGCTGTGGAAAACCTCC TGACCCTAGATCGCCTGCGTCAGGAAGTGGCCGTGAAGGAGCAGCTGACAGGAAAAGGGAAGCTGAACCG GAGGAGTATCAGCTCTCCTAGCATGAACAGATTGTCAGGAAGCCGACAGGAGCTTAGCCCATCCCACAGT CTAAGCAGCAACAAGCCCGTGCCCCGAATCTTGGTGCAGCCTACTTTCTCAGATGCCCGGGCAACAAGGA CAGAGGAGGCTCAGCAGGGCAGCCCTGGGCCTAGTGGGGCTTTGGAGTCTATGGTGAAGATGGCGGCCCC TACAGTAAAGATCTGCGACAAACCTGTCAGAGTGTCTTCCCCTCCATCTACCATGGTAGTTACTCAGCCC CAAGAAGGGCAGGATGGACCTCCCAGCCCCTTGAGCGAAGCTTCAAGTGGCTACTTCTCTCACAGTGTCT CCACTGCCACCCTGTCAGAAACGCTCACCTTAGGCCTGGACACCACAGGCCTCGGCAGCCAGACACCTGG ATCCCCCCCTGCCCTGTGTCAGGTCACCCAAGAGCCTGAGCTGGCTTTCCTCTCCTGCACACAGAGCCAT CCAACTGACCCAGAGGAGCCCCATATACCACCTGCAACTCCTACTCAAAGCACAGAGCTGGAGGTCCCCA GACCGCCTCTCCTTTCTGACCCCACACCTGCTGTGCCCACGTCTCCATTCAGAATCCGGAAAGTGCGGCC CTCAGAGCTTACATCCTTCACAGGCATGCTGGGGGGAGCCTCCTCTGGAGCACAGGAGGACCCAGTGGTT TCAGAAGACCCGAGCCATGCTAGGGGACAGACTCTAGGGAGGCTAGAAGTGACATCTGACTCTGAGGATG CCAGCGAAGTTCCTGAGTGGCTCAGAGAGGGAGAGTATGTTGTTGTGGGCACCAACAAGACGGGCATTGT AAGATATATTGGACCAACTGATTTCCAGGAGGGAACGTGGATTGGTGTGGAGCTGGACCTGCCTGCAGGA AAGAATGATGGCTCCATTGGTGGGAAACAGTATTTCAGATGCAACCCTGGTTACGGGCTGCTGGTGAGGC CAGGCCGGGTCCGCAGGGCTGCAGGCACTGGGCGCAGGCGCAGTTCAGGGCTCCAGCCTCAGGGAGCCCC AGAGGCCCGCAGGAGTGCTACTATCTCGGGCTCTGCCACCAACCTGGCTTCACTGACAGCTGCATTGGCC AAGGGAGACAGGAGCTACAAGAATCCTGAGAACCGGAAGTCCTGGGCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212134 representing NM_213626 Red=Cloning site Green=Tags(s) MGDSKVKVAVRVRPMNRREIDLHTKCVVDVEANKVILNPINTNLSKGDARGQPKIFAYDHCFWSMDESVR EKYAGQDDVFKCLGENILQNAFDGYNACIFAYGQTGSGKSYTMMGTADQPGLIPRLCSGLFERTQKEENE EQSFKVEVSYMEIYNEKVRDLLDPKGSRQTLKVREHSVLGPYVDGLSKLAVTSYKDIESLMSEGNKSRTV AATNMNEESSRSHAVFKITLTHTLYDVKSGTSGEKVGKLSLVDLAGSERATKTGAAGDRLKEGSNINKSL TTLGLVISALADQGAGKNKNKFVPYRDSVLTWLLKDSLGGNSKTAMVATVSPAADNYDETLSTLRYADRA KHIINHAVVNEDPNARIIRDLREEVEKLREQLTKAEAMKSPELKDRLEESEKLIQEMTVTWEEKLRKTEE IAQERQKQLESLGISLQTSGIKVGDDKCFLVNLNADPALNELLVYYLKEHTLIGSANSQDIQLCGMGILP EHGIIDIMPEGQVMLTPQKNTRTFVNGSSVSSPIQLHHGDRILWGNNHFFRLNLPKKKKKAEREDEEREA SLKNDSSSEQLDADGDSSSEVSSEINFNFEYAQMEVTMKALGSNDPMQSILSSLEQQHEEEKRSALERQR LMYEHELEQLRRRLSPERQNCRGVDRLSFHSPSAQQRLRQWAEEREATLNNSLMRLREQIVKANLLVREA SYIAEELDKRTEYKVTLQIPASSLDANRKRGSLLSEPAIQVRRKGKGKQIWSLEKLENRLLDMRDLYQEW KECEEDSPVSRSYFKRADPFYDEQENHSLIGVANVFLETLFYDVKLQYAVPIINQKGEVAGRLHVEVMRL SGAIGERIAGGDDPTEVSSEKEVQENRLVCMVKILQATGLPQHLCHFVFCKYDFWDQQEPVTVAPEVDTS SSPTSKEPQCMVVFDHCSEFSVNITEDFIEYLSEGALAIEVYGHKMNDPRKNPALWDLGIIQAKTRSLRD RWSEVTRKLEFWVQILEQNENGEYCPVEVIAAKDVPTGGIFQLRQGQSRRVQVEVKSVQESGTLPLMEEC ILSVGIGCVKVRPLRSPKTHENIHEEEEDMDSYQDRDLERLRRKWLNALTKRQEYLDQQLQKLVSKHDKT EDDADREAQLLEMRLTLTEERNAVMVPSAGSGIPGAPAEWTPVPGMEAHIPVVFLDLNADDFSSQDNLDD PEAGWDATLTGEEEEEFFELQIVKHHDGEVKAEASWDSAVHNCPQLSKGTPADERVFLILRVAVQLSHPA DMQLVLRKRICVHVHGRQGFAQSLLKKMTHRSSIPGCGVTFEIVSNIPEVDAQGVEEREALARMAANVEN AASADSEACIEKYLRSVLAVENLLTLDRLRQEVAVKEQLTGKGKLNRRSISSPSMNRLSGSRQELSPSHS LSSNKPVPRILVQPTFSDARATRTEEAQQGSPGPSGALESMVKMAAPTVKICDKPVRVSSPPSTMVVTQP QEGQDGPPSPLSEASSGYFSHSVSTATLSETLTLGLDTTGLGSQTPGSPPALCQVTQEPELAFLSCTQSH PTDPEEPHIPPATPTQSTELEVPRPPLLSDPTPAVPTSPFRIRKVRPSELTSFTGMLGGASSGAQEDPVV SEDPSHARGQTLGRLEVTSDSEDASEVPEWLREGEYVVVGTNKTGIVRYIGPTDFQEGTWIGVELDLPAG KNDGSIGGKQYFRCNPGYGLLVRPGRVRRAAGTGRRRSSGLQPQGAPEARRSATISGSATNLASLTAALA KGDRSYKNPENRKSWAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_213626
ORF Size	5301 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_213626.1, NP_998791.1
RefSeq Size	5304 bp
RefSeq ORF	5304 bp
Locus ID	305967
MW	196.6 kDa
Gene Summary	human homolog has microtubule stimulated ATPase activity; binds the human discs large (Dlg) tumor suppressor homolog; may play a role in intracellular transport of Dlg [RGD, Feb 2006]

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