Smc2 (NM_001108666) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CAP-E; CAPE; Smc2l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211255 representing NM_001108666 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGTTAAATCAATCATTCTCGAAGGATTCAAGTCCTATGCACAAAGGACTGAAGTCAATGGTTTTG ACCCCCTCTTCAATGCGATCACTGGTTTGAATGGTAGTGGAAAATCCAATATATTGGATTCCATATGCTT TCTCCTAGGCATCTCCAACCTGTCTCAGGTTCGGGCTTCAAATTTACAAGATCTGGTTTATAAAAACGGA CAGGCTGGAATCACCAAAGCCTCTGTTTCAATCACTTTTGATAATTCTGACAAAAAGCAGAGTCCATTAG GATTTGAAGCTCATGATGAAATCACTGTAACAAGACAGGTGGTTATTGGTGGCAGAAATAAATACTTAAT CAATGGAGTGAATGCCAACAACACCAGAGTCCAGGATCTCTTTTGTTCTGTGGGCCTGAACGTGAATAAC CCTCACTTTCTCATCATGCAGGGCAGAATTACGAAAGTATTAAATATGAAACCACCAGAGATATTATCCA TGATCGAAGAAGCTGCTGGAACCAGGATGTATGAGTACAAAAAAATAGCTGCCCAGAAAACTATAGAAAA AAAGGAGGCTAAGCTGAAAGAAATAAAAACGATACTGGAAGAAGAGATTACGCCAACAATTCAAAAATTA AAAGAGGAAAGATCTTCTTACTTGGAGTACCAGAAAGTGATGCGTGAGATCGAGCATCTGAGCCGTTTAT ATATTGCCTATCAGTTTTTGCTGGCTGAAGATACCAAGGAACGCTCAGCTGGGGAGCTAAAGGAAATGCA GGATAAAATATTAAAGCTTCAAGAAGTACTGTCAGAGAATGAAAAAAAGATAAAGGCTCTCAATTGTGAA ATAGAAGAATTGGAGAAGAGAAAAGATAAGGAAACTGGAGGCATCCTCCGATCTTTAGAGGATGCTTTTG CAGAAGCTCAGCGAGTTAATACTAAGTCTCAAAGTGCATTTGACCTCAAGAAGAAAAACCTAGCCAGTGA AGAAACCAAGCGCAAAGAGCTAGAGAAAAACATGGCTGAGGACTCTAAAGCATTAGCAGCAAAGGAAAAG GAAGTTAAGAAGCTAACCGATGGACTGCACGGCCTTCAAGAAGCAAGCAATAAAGATGCGGAAGCTCTGG CCGCCGCCCAGCAGCACTTCAATGCAGTGTCTGCTGGCTTGTCCAGTAATGAAGATGGAGCAGAGGCAAC TCTGGCCGGGCAGATGATGGCCTGTAAGAACGACATCAGCAAAGCGCAGACCGGGGCCAAGCAGGCTCAG ATGAAGTTGAAACATGCCCAACAGGAACTGAAGAATAAACAAGCAGAAGTTAGGAAGATGGATAGTGGGT ACAAGAAGGATCAAGAAGCTTTTGAAGCTGTGAAAAAAGTTAAAGAAAAACTTGAAACCGAAATGAAGAA GCTAAATTACGAAGACAACAAAGAGGAAAGGCTTTTGGAAAAGCACAGGCAGGTGTCTCGTGACATCAGT AATCTAAAAGGAACCTACGAAGCTCTCTTGGCCAAGTTTCCCAATCTTCGATTTGCATACAAGGACCCAG AGAAGAACTGGAATCGAAATTGTGTAAAAGGACTTGTAGCTTCTCTGATAAATGTGAAAGATAATTCTAC AGCCACAGCTCTGGAATTGGTGGCTGGAGAACGACTCTACAATGTTGTAGTAGACACAGAGGTTACTGGT AAGAAACTATTAGAAAAGGGGGAATTGAAGCGTCGATACACAATAATTCCTCTCAATAAGATTTCAGCCA GATGTATTGCTCCTGAAACTCTGAGGGTCGCTCAGAATCTTGTTGGTCCTAACAATGTTCATGTAGCTCT TTCCTTGGTTGACTACAAACCAGAACTTCAGAAAGCGATGGAATTTGTCTTCGGAACAACATTTGTTTGT AACAATATGGATAATGCCAAAAAAGTGGCCTTTGATAAAAGAATAATGACCAGAACTGTAACCCTTGGGG GTGATGTATTTGATCCTCATGGAACATTGAGTGGAGGTGCTAGGTCCCAGGCGGCTTCTATTTTAACCAA GTTTCAAGAACTCAAAGATGTTCAAGCTGAGCTGAGGACAAAGGAGAAAGAACTGCAGGCTCTGGAAGAG GAATTGGCTGGTCTTAAAAATGTCGCTGAAAAGTATCGCCAACTGAAACAGCAGTGGGAGATGAAGACAG AGGAGGGAGATTTGTTGCAAACCAAACTTCAGCAAAGCTCTTACCATAAGCAGCAAGAAGAATTAGATGC CCTTAAAAAAACCATTGAGGAAAGTGAGGAGACATTAAAAAACACAAAAGAAATCCAAAAGAAAGCTGAA GAAAAATATGAGGCATTGGAGAATAAAATGAAAAATGCAGAAGCTGAAAGAGAGAAAGAACTGAAGGATG CTCAGAAAAAACTGGATTGTGCCAAAACAAAGGCTGATGCATCTAGCAAGAAAATGAAAGAAAAGCAACA GGAGGTTGAAGCTATCACTCTGGAGCTAGAGGAGCTCAAAAGAGAGCATGCATCTAACGAACAGCAGCTT GATGCTGTGAATGAAGCCATCAAAGCCTATGAAGGGCAGATTGAAATAATGGCAGCTGAGGTGGCCAAAA ACAAGGAATCAGTTAATAAAGCTCAAAATGAGCTGATGAAGCAAAAAGAAATAATAAGTGCCCAGGACAA CATAATTAAAGATAAATGTGCGGAAGTGGCAAAACACAATTTGCAAAACAATGATTCTCAGCTTAAAATT AAGGAGTTAGACCACAGCATAAGCAAACACAAGCGGGAAGCTGACGACGCTGCTGCGAAGGTATCCAAAA TGTTGAATGATTATGACTGGATTAATGCTGAGAAACACCTCTTTGGCCAACCTAATAGTGCCTATGATTT CAAAACAAACAACCCCAAAGAAGCTGGCCAGAGACTTCAGAAGTTGCAAGAAGTAAAAGAGAAACTAGGG AGAAATGTCAACATGAGAGCTATGAATGTGCTGACAGAAGCGGAAGAGCGGTATAATGATTTGATGAAGA AGAAAAGAATTGTAGAAAATGACAAATCCAAAATCCTTGCAACTATAGAAGATCTTGACCAGAAGAAAAA TCAGGCCCTAAATATTGCTTGGCAAAAGGTGAACAAAGACTTTGGCTCTATTTTTTCTACCCTTTTGCCT GGTGCCAATGCTATGCTTGCACCACCAGAAGGGCAGACAGTTTTAGATGGTCTGGAGTTCAAGGTTGCCT TAGGAAATACATGGAAAGAAAACCTAACTGAGCTTAGTGGTGGTCAGAGGTCTCTGGTGGCTCTCTCACT AATATTGTCCATGCTCCTCTTCAAACCTGCTCCAATTTATATCTTGGACGAGGTGGATGCGGCCTTAGAT CTTTCTCATACCCAGAACATCGGACAGATGCTGCGAACTCACTTCACACATTCTCAATTCATTGTGGTAT CCCTCAAAGAAGGTATGTTCAACAATGCAAACGTTCTCTTCAAAACCAAGTTTGTGGATGGTGTTTCTAC AGTAGCCAGATTTACACAGAGTCAAAATGGGAGGATTCCAAAGGAGGCAAAGGCCAAGGGCAAAGGACCC AGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211255 representing NM_001108666 Red=Cloning site Green=Tags(s) MYVKSIILEGFKSYAQRTEVNGFDPLFNAITGLNGSGKSNILDSICFLLGISNLSQVRASNLQDLVYKNG QAGITKASVSITFDNSDKKQSPLGFEAHDEITVTRQVVIGGRNKYLINGVNANNTRVQDLFCSVGLNVNN PHFLIMQGRITKVLNMKPPEILSMIEEAAGTRMYEYKKIAAQKTIEKKEAKLKEIKTILEEEITPTIQKL KEERSSYLEYQKVMREIEHLSRLYIAYQFLLAEDTKERSAGELKEMQDKILKLQEVLSENEKKIKALNCE IEELEKRKDKETGGILRSLEDAFAEAQRVNTKSQSAFDLKKKNLASEETKRKELEKNMAEDSKALAAKEK EVKKLTDGLHGLQEASNKDAEALAAAQQHFNAVSAGLSSNEDGAEATLAGQMMACKNDISKAQTGAKQAQ MKLKHAQQELKNKQAEVRKMDSGYKKDQEAFEAVKKVKEKLETEMKKLNYEDNKEERLLEKHRQVSRDIS NLKGTYEALLAKFPNLRFAYKDPEKNWNRNCVKGLVASLINVKDNSTATALELVAGERLYNVVVDTEVTG KKLLEKGELKRRYTIIPLNKISARCIAPETLRVAQNLVGPNNVHVALSLVDYKPELQKAMEFVFGTTFVC NNMDNAKKVAFDKRIMTRTVTLGGDVFDPHGTLSGGARSQAASILTKFQELKDVQAELRTKEKELQALEE ELAGLKNVAEKYRQLKQQWEMKTEEGDLLQTKLQQSSYHKQQEELDALKKTIEESEETLKNTKEIQKKAE EKYEALENKMKNAEAEREKELKDAQKKLDCAKTKADASSKKMKEKQQEVEAITLELEELKREHASNEQQL DAVNEAIKAYEGQIEIMAAEVAKNKESVNKAQNELMKQKEIISAQDNIIKDKCAEVAKHNLQNNDSQLKI KELDHSISKHKREADDAAAKVSKMLNDYDWINAEKHLFGQPNSAYDFKTNNPKEAGQRLQKLQEVKEKLG RNVNMRAMNVLTEAEERYNDLMKKKRIVENDKSKILATIEDLDQKKNQALNIAWQKVNKDFGSIFSTLLP GANAMLAPPEGQTVLDGLEFKVALGNTWKENLTELSGGQRSLVALSLILSMLLFKPAPIYILDEVDAALD LSHTQNIGQMLRTHFTHSQFIVVSLKEGMFNNANVLFKTKFVDGVSTVARFTQSQNGRIPKEAKAKGKGP S TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108666
ORF Size	3573 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108666.3, NP_001102136.2
RefSeq Size	5273 bp
RefSeq ORF	3576 bp
Locus ID	362519
MW	134.3 kDa

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