Tpbg (NM_031807) Rat Tagged ORF Clone

CAT#: RR211234

  • TrueORF®

Tpbg (Myc-DDK-tagged ORF) - Rat trophoblast glycoprotein (Tpbg), (10 ug)



  "NM_031807" in other vectors (3)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms 5T4; WAIF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR211234 representing NM_031807
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGGGGCGGGCTCCCGGGGCCCCTCTGCCGGGGACGGACGGCTGCGGTTGGCAAGGCTGGCGCTAG
TGCTGCTGGGTTGGGTCTCCGCGTCGGCCCCCAGCTCTTCGCTACCCTCGTCTTCCACCTCCCCGGCTGC
CTTCCTGGCTTCGGGGTCTGCGCAGCCTCCGCCGGCCGAGCGATGCCCCGCTGCGTGCGAGTGCTCCGAG
GCGGCGCGCACGGTTAAGTGCGTGAACCGCAACCTGCTGGAGGTGCCTGCGGACCTGCCACCTTACGTGC
GCAACCTTTTCCTCACTGGCAACCAGATGACCGTGCTCCCGGCTGGCGCCTTCGCCCGCCAGCCGCCGCT
CGCCGACCTGGCAGTGCTCAACCTCAGCGGCAACCACCTGAAGGAGGTGGGTGCAGGTGCCTTCGAGCAT
CTGCCCGGCCTTCGCCGGCTTGACCTCAGCCACAATCCTCTCACCAACCTCAGCGCCTTCACCTTCGCGG
GCAGCAACGTCAGCGTCTCGACCCCCAGCCCCCTATTGGAGCTGATCTTGAATCACATCGTGCCCCCTGA
GGATCAGCGGCAGAATGGGAGCTTCGAGGGCATGGTGGCCTTCGAGGGCATGGTGGCAGCTGCCCTGCGC
TCAGGCCTTGCACTCCGAGGGCTGCATCACCTAGAGCTGGCCAGCAATCACTTTCTTTACCTGCCTCGGG
ACTTATTGGACCAACTGCCAAGTCTCAAGCACCTGGACCTCAGGAACAACTCCCTGGTGAGCCTGACCTA
TGCATCTTTCCGCAACCTGACACACCTCGAAAGCCTCCACTTGGAGGACAATGCCCTCAAGGTCCTTCAC
AACTCCACCTTGGCTGAGTGGCAAGGCCTGGCTCATGTCAGGGTGTTCCTGGACAACAATCCCTGGGTTT
GTGACTGCTACATGGCTGACATGGTGTCTTGGCTTAAAGAGACAGAGGTGGTGCCGGATAAAGCCAGGCT
CACCTGCGCATTCCCAGAAAAAATGAGGAACCGTGGCCTCTTGGACCTCACCAGCTCTGACCTGGACTGT
GACGCTACCCTTCCCCAATCCCTGCAGACTTCCTATGTCTTCTTAGGTATTGTTTTAGCTCTGATAGGCG
CTATTTTCCTCCTCGTTTTGTATTTGAACCGGAAAGGCATAAAAAAGTGGATGCATAACATCAGAGATGC
CTGCAGGGATCACATGGAAGGGTATCATTACAGATACGAAATCAATGCGGACCCCAGCTTAACAAATCTC
AGTTCCAACTCGGATGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR211234 representing NM_031807
Red=Cloning site Green=Tags(s)

MPGAGSRGPSAGDGRLRLARLALVLLGWVSASAPSSSLPSSSTSPAAFLASGSAQPPPAERCPAACECSE
AARTVKCVNRNLLEVPADLPPYVRNLFLTGNQMTVLPAGAFARQPPLADLAVLNLSGNHLKEVGAGAFEH
LPGLRRLDLSHNPLTNLSAFTFAGSNVSVSTPSPLLELILNHIVPPEDQRQNGSFEGMVAFEGMVAAALR
SGLALRGLHHLELASNHFLYLPRDLLDQLPSLKHLDLRNNSLVSLTYASFRNLTHLESLHLEDNALKVLH
NSTLAEWQGLAHVRVFLDNNPWVCDCYMADMVSWLKETEVVPDKARLTCAFPEKMRNRGLLDLTSSDLDC
DATLPQSLQTSYVFLGIVLALIGAIFLLVLYLNRKGIKKWMHNIRDACRDHMEGYHYRYEINADPSLTNL
SSNSDV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031807
ORF Size 1278 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031807.1, NP_113995.1
RefSeq Size 2333 bp
RefSeq ORF 1281 bp
Locus ID 83684
UniProt ID Q5PQV5
MW 46.9 kDa
Gene Summary mouse homolog is a highly N-glycosylated transmembrane glycoprotein [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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