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Bhmt (NM_030850) Rat Tagged ORF Clone
CAT＃: RR210729
- TrueORF®
Bhmt (Myc-DDK-tagged ORF) - Rat betaine-homocysteine methyltransferase (Bhmt), (10 ug)
"NM_030850" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210729 representing NM_030850
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCGATTGCCGGCAAGAAGGCCAAGAGGGGAATCTTAGAACGCTTAAATGCTGGCGAAGTCGTGA TCGGAGATGGGGGATTTGTCTTTGCACTGGAAAAGAGGGGCTACGTAAAGGCTGGACCCTGGACCCCAGA GGCTGCGGTGGAGCACCCCGAGGCAGTTCGGCAGCTTCATCGGGAGTTCCTCAGAGCTGGATCGAACGTC ATGCAGACCTTCACTTTCTATGCAAGTGAGGACAAGCTGGAAAACCGAGGGAACTACGTGGCAGAGAAGA TATCTGGGCAGAAGGTCAATGAAGCTGCTTGTGACATTGCACGGCAAGTTGCTGACGAAGGGGATGCATT GGTTGCAGGAGGTGTGAGTCAGACACCTTCCTACCTCAGCTGCAAGAGTGAGACGGAAGTTAAAAAGATA TTTCACCAACAGCTTGAGGTCTTCATGAAGAAGAATGTGGACTTCCTCATTGCAGAGTATTTTGAACATG TTGAAGAAGCCGTGTGGGCAGTCGAGGCCTTAAAAACATCCGGGAAGCCTATAGCGGCTACCATGTGCAT CGGACCTGAAGGAGATCTACATGGCGTGTCTCCTGGAGAGTGCGCAGTGCGTTTGGTAAAAGCAGGTGCC GCCATTGTCGGTGTGAACTGCCACTTCGACCCCAGCACCAGCTTGCAGACAATAAAGCTCATGAAGGAGG GTCTGGAAGCAGCTCGGCTGAAGGCTTACTTGATGAGCCACGCCCTGGCCTACCACACCCCTGACTGTGG CAAACAGGGATTTATTGATCTCCCAGAATTCCCCTTTGGATTGGAACCCAGAGTTGCCACCAGATGGGAT ATTCAAAAATACGCCAGAGAGGCCTACAACCTGGGGGTCAGGTACATTGGCGGCTGCTGCGGATTTGAGC CCTACCACATCAGGGCCATTGCAGAGGAGCTCGCCCCAGAAAGGGGATTTTTACCACCAGCTTCAGAAAA ACATGGCAGCTGGGGAAGTGGTTTGGACATGCACACCAAACCCTGGATCAGGGCAAGGGCCAGGAAAGAA TACTGGCAGAATCTTCGAATAGCTTCGGGCAGACCGTACAATCCTTCGATGTCCAAGCCGGATGCTTGGG GAGTGACGAAAGGGGCAGCAGAGCTGATGCAGCAGAAGGAAGCCACCACTGAGCAGCAGCTGAGAGCGCT CTTCGAAAAACAAAAATTCAAATCCGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210729 representing NM_030850
Red=Cloning site Green=Tags(s) MAPIAGKKAKRGILERLNAGEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNV MQTFTFYASEDKLENRGNYVAEKISGQKVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKI FHQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKTSGKPIAATMCIGPEGDLHGVSPGECAVRLVKAGA AIVGVNCHFDPSTSLQTIKLMKEGLEAARLKAYLMSHALAYHTPDCGKQGFIDLPEFPFGLEPRVATRWD IQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARKE YWQNLRIASGRPYNPSMSKPDAWGVTKGAAELMQQKEATTEQQLRALFEKQKFKSAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030850 |
| ORF Size | 1221 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030850.1, NP_110477.1 |
| RefSeq Size | 1884 bp |
| RefSeq ORF | 1224 bp |
| Locus ID | 81508 |
| UniProt ID | O09171 |
| MW | 45 kDa |
| Gene Summary | displays differential expression in a model of liver cirrhosis [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210729 | Bhmt (untagged ORF) - Rat betaine-homocysteine methyltransferase (Bhmt), (10 ug) |
CNY 3,990.00 |
|
| RR210729L3 | Lenti ORF clone of Bhmt (Myc-DDK-tagged ORF) - Rat betaine-homocysteine methyltransferase (Bhmt), (10 ug) |
CNY 6,080.00 |
|
| RR210729L4 | Lenti ORF clone of Bhmt (mGFP-tagged ORF) - Rat betaine-homocysteine methyltransferase (Bhmt), (10 ug) |
CNY 6,650.00 |
