Tonsl (NM_001130572) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nfkbil2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210543 representing NM_001130572 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCTGGAACAGGAGCTTCGCCAGCTGAGCAAGGCGAAGGCCAGGGCCCAGAGGAATGGACAGCTGT GCGAGGAGGCGGTCTGCTGCCACCAGCTGGGGGAGCTGCTAGCTAGCCACGGTCGCTTCCAGGAAGCTTT AGAGGAGCACCAGCAGGAGCTACATCTGCTAGAGAGTGTCCAGGACACCCTAGGCTGCGCTGTGGCCCAC CGCAAGATCGGAGAGCGGCTGGCTGAAATGGAGAACTACTCTGCTGCTCTGAAGCACCAGCATCTCTACC TGGATCTAGCTGGTTCCCTGTCCAACCACACTGAATTGCAGAGAGCCTGGGCCACCATTGGCCGCACCCA TCTGGATGTATATGACCACTGCCAATCAAGAGATTCCTTGCTGCAGGCACAGGCTGCCTTTGAGAAGAGT TTGGCTATTGTGGATGAGAAACTAGAGGGGATGCTGACCCAGCGGGAACTGAGTGAGATGAGGACCCGTC TCTACCTCAATCTGGGCCTCACCTGTGAGAGCCTGCAGCAGACAGCCCAGTGCAACAACTACTTCAAAAA GAGCATCTTTCTCGCTGAGCAGAACCATCTGTATGAAGATCTGTTCCGGGCCCGATACAACCTGGGTGCC ATCCACTGGCGCGGAGGGCAGCACTCTCAGGCCATGCGCTGCTTGGAGGGGGCCCGGGAATGTGCACGTG CCATGAAGATGAGATTCATGGAAAGCGAATGTTGCATGCTAGTGTCTCAGGTGCTCCAAGATCTGGGGGA CTTTCTGGCTGCCAAACGAGCCCTGAAGAAGGCCTATAGGTTGGGCTCTCAGAAGCCTAACCAGAGAGTG GCTATCTGTCAGAGTCTCAAGTATGTATTGGCCGTGGTCCGGCTGCAGCAGCAGCTGCAAGAGGCTGAGG GCAATGATCTTCAAGGTGCCATGGCTATCTGTGAACAGTTGGGGGACCTTTTCTCCAAAGCCGATGACTT CCCCAAGGCATCTGAGGCTTACCAGAAGCAGCTGCACTTTGCTGAGCTGCTGAACAGACCGGACCTCGAG CTGGCTGTCATCCACGAATCCCTGGCCACCACGCTGGGAGACATGAAGGATTACCACAAGGCTGTACACC ACTATGAAGAGGAACTGAGGCTACGCAAAGGCAATGCCCTGGAGGAAGCTAAGACTTGGTTCAACATTGG ACTGGCCCGTGAGGAAGCTGGAGATGCATATGAGCTGCTAGCTCCATGCTTCCAGAAGGCTTTCGGCTGT GCTCAGCAGGCCCAGCGGTACCAACTGCAGAGGCAGATCTTACAGCATCTTTACACTGTGCAACTGAAGT TGCAGCCCCAAGAAGCCCGTGACACTGAAATCAGACTGCAGGAGCTGAGTATGGCAAAAGACACAGAGGA AGAGGAGGAAGAAGAGGAGGAGGAAGAGGAAGAAGCCAGTGAGGCCCTGGAGACCAGTGAAATGGAGCTC TCAGAGAGCGAGGATGATGCTGACGGCCTGTCTCAGCAGCTGGAGGAAGAGGAGGAGCTTCAGGGCTGTG TGGGGCGGCGGAAGATAAACAAGTGGAACCGGCGCAATGACATGGGAGAGACCCTGTTGCACCGAGCTTG CATCGAAGGCCAACTGCGCCGTGTCCAGGATCTTGTGAAGCAGGGCCATCCCCTGAATCCCCGAGACTAC TGTGGCTGGACTCCTCTACATGAAGCTTGCAACTATGGACATCTTGAGATTGTGCGCTTCCTTCTGGACC ATGGAGCAGCAGTGGATGACCCGGGTGGCCAGGGGTGTGATGGCATCACCCCCCTGCATGATGCCCTCAA CTGTGGCCACTTTGAGGTAGCTGAGCTCCTCATTGAGCGAGGGGCGTCCGTAACTCTCCGCACCAGGAAG GGCCTCAGCCCACTGGAGACACTGCAGCAGTGGGTGAAGCTGTACTTCAGGGATCTTGACCTTGAGACAA GACAGAAGGCCGCATCCATGGAGAGGAGGCTCCAGATGGCCTCCTCAGGCCAAGCTTCCCACAGCTCCCC TGCCCTCCAGACCATTCCAAATAATCATCTCTTCGACCCTGAGACCTCTCCTCCCTCAAGCCCCTGTCCA AAACCCCCCTCGTATACTCCTAGACCTCCAGAGGCCTCTCCAGCCCCTGCTAAGGTCTTTCTGGAGGAAA CTGTGTCTGCAGTGTGCAGACCTCGAAAGAACAGGCACAGGCCAGCCAGCAGCAGTAGCAGCTCGGAGGA CGATGACGACAACACAAACCCCTGCAGGCCATCTCAGAAAAGACTGAGACACAGCACACAGCAGGGTGAA GCCAAGACACCTGACCCATCCAAAAGTAGAGAGACAGCCATTTCAAGCGCTTGCCGGGCTGCTTACCAAG CAGCCATCCGAGGCGTGGGGAGTGCCCAGAGCCGTCGCTTGGTACCTAGCCTGCCTCGGGGCTCAAATGA AGTCCCTGCCCCCAAAACAGCACTCATTCCTGAGGAGGAATTCCTGGCTGAGGAATGGCTGGAGGTAGAT ACACCTCTGACCCGCAGCAGCAGCAGCAGCAGGCCCAGTACCTCCATATCAGACTATGAGCGATGCCCTG CAAGGCCCCGGACTCGTGCCAAGCAGAGTCGCCCAGCAAGTCTTGATGGTTGGTGTACACGGACTAAAGC AGCAGATGCCAGCTTGACTGCAGAGCCTACGGAGAACTCCAGCATGCCAAGGACCACAGGACCCAACAAA GAAAATTGTGCAGCAGGCCAGCCTTTGCTTCTGGTCCAGCCCCCTCCTATCCGGGTTCGAGTTCAAATTC AGGACAACCTTTTCCTCATCCCCGTCCCACACAGCGATGTCCATTCTGTGGCTTGGCTAGCAGAGCAAGC GGCCCAGCGCTACTTCCAAACTTGCGGGCTACTCCCAAGGCTCACCCTACGGAAGGATGGAGCACTACTG GCCCCACAGGACCCCATCCCTGATGTGCTGCAAAGTAACGACGAGGTGATGGCTGAAGTAACGTCATGGG ACCTTCCTCCACTAAAGGACCGCTACCGTAGGGCCTGCCAGAGCCTGGGGCAAGGGGAGCACCAGCAAGT GCTGCAGGCCATGGAGCACCAGAGTTCGAGCCCTTCGTTTAGTGCCTGTTCTCTGGCCTTGTGCCAAGCC CAGCTCACACCCCTGCTGAGGGCCCTCAAGTTACACACAGCACTCCGGGAGCTACGCCTTTCTGGGAACC GACTGGGTGACCCATGTGCTACTGAGCTACTGGCTACCCTGGGCACCATGCCTAACCTGGTCCTCCTCGA TCTCTCTTCCAATCACTTGGGCCCGGAAGGCCTGCGTCAGCTTGTTGAAGGCTCCTTGGGCCAGACTGCT TTCCAGAACGTGGAGGAACTAGACTTGAGTATGAATCCACTGGGAGATGGCTGTGCTCAGGCCCTGGCCT CCCTTCTGCGGACCTGCCCCGTGCTCAGGACCTTGCGTCTACAAGCCTGTGGTTTCAGCCCCAGCTTCTT CCTGAGCCACCAGGCTGCCCTGGGTAGTGCCTTCAAAGATGCTGAGCACCTCAAGACCTTGTCCCTATCT TACAATACTCTCGGCGCTCCTGCCCTGGCAAGGGTGCTGCAGAGCTTACCCACCTGTACCCTCCTACATT TGGAGCTTAGCTCCGTGGCAGCCAGCAAGAGCAACTCGAGTCTCATAGAGCCTGTTATCAAATACTTGAC CAAGGAAGGCTGTGCTCTGGCCCACCTAACCCTGTCTGCAAACTGCCTGAGTGACAAGGCTGTGAGAGAA CTGAGCAGATGTCTCCCTAGCTGCCCCTCACTCACCTCTCTGGACCTGTCCGCCAACCCTGAGGTCAGCT GTGCCGGTCTGGAGGAGCTCCTGTCTGCCCTACAGGAGCGGCCCCAAGGCCTCAGCTTCTTTGACTTGTC AGGCTGCTCCATTCAGGGGCCCCTGAACTCTGACCTCTGGGACAAGATCCTCTCGCAGTTGCAGGAGCTG CAGTTGTGCAGCAAAGACCTGACCACAAAAGACCGAGACACCCTGTGTCAGAGACTGCCGGCGGGTGCGT GCACACTGAACCAAGGTTCCAAGCTCTTCTTTAAATGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210543 representing NM_001130572 Red=Cloning site Green=Tags(s) MTLEQELRQLSKAKARAQRNGQLCEEAVCCHQLGELLASHGRFQEALEEHQQELHLLESVQDTLGCAVAH RKIGERLAEMENYSAALKHQHLYLDLAGSLSNHTELQRAWATIGRTHLDVYDHCQSRDSLLQAQAAFEKS LAIVDEKLEGMLTQRELSEMRTRLYLNLGLTCESLQQTAQCNNYFKKSIFLAEQNHLYEDLFRARYNLGA IHWRGGQHSQAMRCLEGARECARAMKMRFMESECCMLVSQVLQDLGDFLAAKRALKKAYRLGSQKPNQRV AICQSLKYVLAVVRLQQQLQEAEGNDLQGAMAICEQLGDLFSKADDFPKASEAYQKQLHFAELLNRPDLE LAVIHESLATTLGDMKDYHKAVHHYEEELRLRKGNALEEAKTWFNIGLAREEAGDAYELLAPCFQKAFGC AQQAQRYQLQRQILQHLYTVQLKLQPQEARDTEIRLQELSMAKDTEEEEEEEEEEEEEASEALETSEMEL SESEDDADGLSQQLEEEEELQGCVGRRKINKWNRRNDMGETLLHRACIEGQLRRVQDLVKQGHPLNPRDY CGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCDGITPLHDALNCGHFEVAELLIERGASVTLRTRK GLSPLETLQQWVKLYFRDLDLETRQKAASMERRLQMASSGQASHSSPALQTIPNNHLFDPETSPPSSPCP KPPSYTPRPPEASPAPAKVFLEETVSAVCRPRKNRHRPASSSSSSEDDDDNTNPCRPSQKRLRHSTQQGE AKTPDPSKSRETAISSACRAAYQAAIRGVGSAQSRRLVPSLPRGSNEVPAPKTALIPEEEFLAEEWLEVD TPLTRSSSSSRPSTSISDYERCPARPRTRAKQSRPASLDGWCTRTKAADASLTAEPTENSSMPRTTGPNK ENCAAGQPLLLVQPPPIRVRVQIQDNLFLIPVPHSDVHSVAWLAEQAAQRYFQTCGLLPRLTLRKDGALL APQDPIPDVLQSNDEVMAEVTSWDLPPLKDRYRRACQSLGQGEHQQVLQAMEHQSSSPSFSACSLALCQA QLTPLLRALKLHTALRELRLSGNRLGDPCATELLATLGTMPNLVLLDLSSNHLGPEGLRQLVEGSLGQTA FQNVEELDLSMNPLGDGCAQALASLLRTCPVLRTLRLQACGFSPSFFLSHQAALGSAFKDAEHLKTLSLS YNTLGAPALARVLQSLPTCTLLHLELSSVAASKSNSSLIEPVIKYLTKEGCALAHLTLSANCLSDKAVRE LSRCLPSCPSLTSLDLSANPEVSCAGLEELLSALQERPQGLSFFDLSGCSIQGPLNSDLWDKILSQLQEL QLCSKDLTTKDRDTLCQRLPAGACTLNQGSKLFFKCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130572
ORF Size	4101 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001130572.1, NP_001124044.1
RefSeq Size	4246 bp
RefSeq ORF	4104 bp
Locus ID	366953
UniProt ID	D4A615
MW	151.8 kDa
Gene Summary	Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold (By similarity).[UniProtKB/Swiss-Prot Function]

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