Smarca2 (NM_001004446) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210465 representing NM_001004446 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACACCCACAGACCCAGCTGCAATGCCCCATCCTGGGCCCTCCCCGGGGCCTGGACCTTCTCCTG GACCAATTCTGGGGCCTAGTCCAGGACCAGGACCGTCCCCAGGTTCTGTGCACAGTATGATGGGGCCCAG TCCTGGACCTCCCAGTGTCTCACATCCTATGTCGACAATGGGCTCGGCAGACTTCCCACAGGAAGGCTTG CACCAGCTGCATAAGCCCATGGATGGGATACATGACAAGGGGATTGTAGAAGGTGTCCACTGTGGATCCA TGAAGGGTACCAGTATGCGCCCACCACACCCAGGAGTGGGCCCTCCACAGAGCCCAATGGATCAGCACAG TCAAGGTTATATGTCACCACATCCATCTCCTTTGGGAGCCCCAGAGCATGTCTCCAGCCCTATATCTGGA GGAGGCCCAACCCCACCCCAGATGCCGCCGAGCCAGCCAGGGACACTCATCCCAGGAGATCTGCAGGCCA TGAACCAGCCTGACAGAGGTCCCTCGCCTTTCAGTCCTGTACAGCTGCATCAGCTTCGAGCTCAGATTTT AGCTTACAAAATGTTGGCCAGGGGCCAGCCCCTCCCTGAAACTCTGCAGCTTGCAGTCCAGGGAAAAAGG ACCTTGCCTGGTATACAACAGCAACAGCAGCAGCAGCAGCAACAACAACAGCAGCAGCAACAACAGCAGC CCCAGCAGCCCCAGCAGCCTCAGCAGCAGACACAGGCACAGCCACAGCAGCAGCAACAGCAGCAGCAGCA GCCGCCAACTCTTGTTAGCTACAATAGACCATCTGGCCCTGGGCAGGAGCTGCTGATGACTGCTCAGAAC ACCCAGCGGAAGCTGTCGGCACCCGCACCCAGCGGCCGACCTTCACCTGCACCTCCAGCGGCTACCCAGC CCACGGCCACAGCAGTGCCTGGGCCCTCGGTGCAGGAGCCTGCCCCAGGGCAGCCATCTCCCGTCCTGCA GCTGCAACAGAAGCAGAGCCGCATCAGCCCCATCCAGAAACCGCAAGGCCTGGACCCCGTGGAGATCCTG CAGGAACGGGAGTACAGACTTCAGGCTCGCATAGCTCATAGGATACAAGAACTGGAAAGTCTGCCTGGCT CCTTGCCACCAGATTTACGAACCAAAGCAACCGTAGAGCTGAAAGCACTTCGCTTGCTCAACTTTCAACG TCAGCTGAGACAGGAGGTGGTGGCCTGCATGCGAAGGGACACCACCCTGGAGACAGCCCTCAACTCCAAG GCGTACAAGCGGAGTAAGCGCCAAACCCTTCGAGAGGCACGCATGACGGAGAAACTGGAGAAGCAGCAGA AGATAGAGCAGGAGAGGAAACGCCGCCAGAAGCACCAGGAATACCTGAACAGTATTTTGCAACATGCAAA AGATTTTAAGGAATACCACCGGTCTGTGGCTGGGAAGATCCAGAAGCTGTCCAAAGCAGTGGCGACTTGG CATGCTAACACCGAAAGGGAGCAGAAGAAGGAGACGGAGCGGATCGAGAAGGAGAGAATGCGAAGGCTGA TGGCTGAAGATGAAGAGGGCTACAGGAAGCTCATCGACCAAAAGAAAGACAGACGCCTGGCCTACCTATT GCAGCAGACTGATGAGTATGTCGCCAATCTGACCAACCTGGTTTGGGAGCACAAGCAGGCCCAAGCAGCC AAAGAGAAGAAGAAGAGGAGGAGGAGGAGGAAGAAGGCCGAAGAGAATGCAGAGGGAGGGGAGCCTGCCC TGGGACCAGATGGAGAGCCAATAGACGAAAGCAGCCAGATGAGCGACCTGCCTGTCAAAGTGACGCACAC GGAAACTGGCAAGGTCCTCTTTGGACCAGAAGCACCCAAAGCAAGTCAGCTGGACGCCTGGCTGGAGATG AATCCTGGTTACGAAGTGGCACCCAGATCGGACAGTGAAGAGAGTGAATCAGACTATGAGGAAGAGGATG AAGAAGAAGAGTCCAGTAGGCAGGAAACAGAGGAGAAGATACTGCTGGATCCAAACAGCAAAGAAGTTTC CGAAAAGGACGCCAGGCAGATCATTGAGACTGCGAAACAGGATGTGGACGACGAGTACAGCATGCAGTAC AGTGCCAGAGGGTCCCAGTCCTACTACACGGTGGCTCACGCCATCTCCGAGAGGGTGGAGAAGCAGTCTG CCCTCCTCATTAATGGCACCCTGAAGCACTACCAGCTACAGGGCCTGGAATGGATGGTTTCCCTGTATAA TAACAATCTGAATGGAATCTTAGCTGATGAAATGGGGCCAGGCAAGACCATCCAGACCATTGCACTCATC ACGTATCTGATGGAGCACAAAGGGCTCAATGGCCCCTACCTCATCATCGTCCCCCTCTCGACTCTGTCTA ACTGGACATATGAATTTGACAAATGGGCTCCTTCTGCGGTGAAAATTTCCTACAAGGGTACCCCTGCCAT GCGACGCTCCCTTGTTCCCCAGTTACGGAGTGGCAAATTCAATGCCCTCCTGACTACCTACGAGTACATT ATAAAAGACAAGCACATTCTTGCAAAGATTAGGTGGAAATACATGATAGTGGACGAAGGTCACCGAATGA AGAACCATCACTGCAAGCTGACCCAGGTCCTGAACACACACTACGTGGCCCCCAGAAGGATCCTTCTGAC TGGGACCCCGCTGCAGAATAAGCTCCCCGAACTCTGGGCCCTCCTCAACTTCCTCCTCCCTACAATCTTC AAGAGTTGCAGCACATTTGAGCAGTGGTTTAATGCTCCGTTTGCCATGACCGGCGAAAGGGTGGACCTGA ACGAAGAAGAAACCATTTTGATCATCAGGCGTCTACACAAGGTGTTGAGACCCTTTTTACTGAGGAGGCT GAAGAAAGAGGTTGAGTCTCAGCTTCCAGAAAAGGTTGAGTATGTGATCAAGTGTGATATGTCCGCTCTG CAGAAGATCCTGTACCGTCATATGCAGGCCAAGGGCATCCTCCTCACGGACGGGTCTGAGAAGGATAAGA AGGGGAAAGGAGGTGCCAAGACGCTTATGAACACTATCATGCAGCTGCGAAAGATATGCAACCACCCATA TATGTTTCAGCACATTGAGGAATCCTTTGCTGAACACCTGGGCTATTCGAATGGGGTCATCAATGGGGCC GAGCTGTATCGGGCCTCAGGAAAGTTTGAGCTACTTGATCGTATTCTGCCCAAATTGAGAGCGACTAACC ACCGCGTGCTGCTTTTCTGCCAGATGACGTCACTCATGACCATCATGGAGGATTACTTTGCTTTTCGGAA CTTCCTTTACCTGCGCCTTGATGGCACCACCAAGTCTGAAGATCGTGCTGCTTTGCTGAAGAAATTCAAT GAACCTGGGTCCCAGTATTTCATTTTCTTGCTGAGCACACGAGCTGGGGGCCTGGGCTTAAATCTTCAGG CCGCAGACACTGTGGTCATCTTTGACAGCGACTGGAATCCTCATCAGGATCTGCAGGCCCAAGACCGAGC TCACCGCATTGGCCAACAAAATGAGGTCCGGGTGCTGAGGCTTTGCACCGTCAACAGTGTTGAGGAAAAG ATTCTCGCGGCTGCCAAGTACAAGCTGAATGTGGACCAGAAGGTTATTCAGGCGGGCATGTTTGACCAGA AGTCATCCAGCCATGAGCGGAGGGCATTCCTACAGGCCATCCTGGAGCACGAAGAGGAGAATGAGGAAGA AGATGAGGTACCAGACGACGAGACCCTGAACCAGATGATTGCTCGCCGGGAAGAAGAGTTTGATCTTTTT ATGCGCATGGACATGGACCGGCGGAGAGAGGACGCCAGAAACCCGAAGCGCAAGCCTCGCTTGATGGAGG AAGATGAGCTGCCCTCCTGGATAATTAAGGATGATGCTGAAGTGGAAAGGCTCACCTGCGAAGAGGAGGA GGAGAAGATATTTGGGAGGGGCTCTCGACAGCGCCGGGATGTGGACTACAGCGATGCCCTCACCGAGAAG CAGTGGCTCAGGGCCATCGAAGACGGAAATTTGGAAGAAATGGAAGAGGAGGTACGGCTGAAGAAGAGGA AAAGACGAAGAAACGTGGATAAAGATCCCGTGAAGGAAGATGTGGAAAAAGCAAAGAAAAGAAGAGGCCG CCCCCCAGCTGAGAAGTTGTCACCAAATCCCCCCAAACTGACGAAGCAGATGAACGCCATCATTGATACT GTGATAAACTACAAGGACAGGTGTAACGTGGAGAAGGTGCCCTGTAATTCTCAGTTGGAAATAGAAGGAA ACAGTTCAGGGCGACAGCTCAGTGAAGTCTTCATTCAGTTACCTTCCAGAAAAGAGTTACCAGAATACTA TGAATTAATTAGGAAGCCAGTGGATTTCAAAAAGATAAAGGAAAGAATCCGTAATCATAAGTACCGAAGC CTGGGAGACCTGGAGAAAGATGTCATGCTTCTCTGTCACAATGCACAGACATTCAACTTGGAAGGATCCC AGATCTACGAAGACTCCATTGTCCTCCAGTCAGTGTTTAAGAGTGCTCGGCAGAAAATTGCCAAAGAAGA AGAGAGTGAGGAGGAAAGCAATGAGGAGGAGGAGGAAGAGGATGAAGAGGAGTCAGAGGCAGAGGCAAAA TCTGTGAAGGTGAAAATCAAACTGAATAAAAAGGAAGAGAAAGGCCGGGATACAGGGAAGGGCAAGAAAA GGCCAAACCGAGGCAAAGCCAAACCTGTTGTGAGCGATTTTGACAGCGATGAGGAGCAGGATGAGAACGA ACAGTCAGAAGCAAGTGGGACTGACGACGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210465 representing NM_001004446 Red=Cloning site Green=Tags(s) MATPTDPAAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPMSTMGSADFPQEGL HQLHKPMDGIHDKGIVEGVHCGSMKGTSMRPPHPGVGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPISG GGPTPPQMPPSQPGTLIPGDLQAMNQPDRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKR TLPGIQQQQQQQQQQQQQQQQQQPQQPQQPQQQTQAQPQQQQQQQQQPPTLVSYNRPSGPGQELLMTAQN TQRKLSAPAPSGRPSPAPPAATQPTATAVPGPSVQEPAPGQPSPVLQLQQKQSRISPIQKPQGLDPVEIL QEREYRLQARIAHRIQELESLPGSLPPDLRTKATVELKALRLLNFQRQLRQEVVACMRRDTTLETALNSK AYKRSKRQTLREARMTEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVAGKIQKLSKAVATW HANTEREQKKETERIEKERMRRLMAEDEEGYRKLIDQKKDRRLAYLLQQTDEYVANLTNLVWEHKQAQAA KEKKKRRRRRKKAEENAEGGEPALGPDGEPIDESSQMSDLPVKVTHTETGKVLFGPEAPKASQLDAWLEM NPGYEVAPRSDSEESESDYEEEDEEEESSRQETEEKILLDPNSKEVSEKDARQIIETAKQDVDDEYSMQY SARGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWMVSLYNNNLNGILADEMGPGKTIQTIALI TYLMEHKGLNGPYLIIVPLSTLSNWTYEFDKWAPSAVKISYKGTPAMRRSLVPQLRSGKFNALLTTYEYI IKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRILLTGTPLQNKLPELWALLNFLLPTIF KSCSTFEQWFNAPFAMTGERVDLNEEETILIIRRLHKVLRPFLLRRLKKEVESQLPEKVEYVIKCDMSAL QKILYRHMQAKGILLTDGSEKDKKGKGGAKTLMNTIMQLRKICNHPYMFQHIEESFAEHLGYSNGVINGA ELYRASGKFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLRLDGTTKSEDRAALLKKFN EPGSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEK ILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEENEEEDEVPDDETLNQMIARREEEFDLF MRMDMDRRREDARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKIFGRGSRQRRDVDYSDALTEK QWLRAIEDGNLEEMEEEVRLKKRKRRRNVDKDPVKEDVEKAKKRRGRPPAEKLSPNPPKLTKQMNAIIDT VINYKDRCNVEKVPCNSQLEIEGNSSGRQLSEVFIQLPSRKELPEYYELIRKPVDFKKIKERIRNHKYRS LGDLEKDVMLLCHNAQTFNLEGSQIYEDSIVLQSVFKSARQKIAKEEESEEESNEEEEEEDEEESEAEAK SVKVKIKLNKKEEKGRDTGKGKKRPNRGKAKPVVSDFDSDEEQDENEQSEASGTDDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001004446
ORF Size	4791 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001004446.1, NP_001004446.1
RefSeq Size	5928 bp
RefSeq ORF	4794 bp
Locus ID	361745
MW	182.2 kDa
Gene Summary	mouse homolog plays a role in regulation of cell proliferation [RGD, Feb 2006]

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