Sptbn1 (NM_001013130) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Spnb1; Spnb2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210353 representing NM_001013130 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACCACGGTGGCCACAGACTATGACAACATTGAGATCCAGCAGCAGTACAGCGATGTGAACAACC GCTGGGACGTGGATGACTGGGACAATGAGAACAGCTCTGCACGGCTCTTTGAGCGCTCCCGCATCAAGGC CCTGGCAGATGAGCGTGAAGCTGTACAGAAGAAGACCTTCACCAAGTGGGTCAACTCCCACCTTGCCCGA GTGTCCTGCCGAATCACAGACCTGTACACTGACCTTCGAGATGGACGGATGCTCATCAAACTACTGGAGG TCCTCTCTGGAGAGAGGCTGCCTAAACCCACTAAGGGACGGATGCGCATCCACTGTCTGGAGAATGTCGA CAAGGCTCTTCAGTTCCTGAAGGAGCAGAGAGTCCATCTCGAGAACATGGGCTCCCATGATATTGTGGAT GGAAACCACCGGCTGACCCTCGGCCTCATCTGGACCATTATCCTGCGCTTCCAGATCCAGGATATCAGTG TGGAGACTGAAGATAACAAAGAGAAAAAGTCTGCTAAGGATGCATTGCTGCTGTGGTGCCAGATGAAGAC AGCTGGGTACCCTAATGTCAACATTCACAATTTCACCACTAGCTGGAGGGATGGCATGGCCTTCAATGCA CTGATACACAAACACCGGCCTGACCTGATAGATTTTGATAAACTGAAGAAATCGAATGCACACTACAATC TGCAGAATGCATTTAACCTGGCAGAGCAGCACCTTGGCCTTACTAAACTGTTAGACCCTGAAGATATCAG CGTGGACCACCCTGATGAGAAGTCCATCATCACGTATGTGGTCACTTACTACCACTACTTCTCCAAGATG AAGGCCTTGGCTGTCGAAGGAAAGCGCATTGGGAAGGTGCTTGATAATGCTATAGAAACAGAGAAAATGA TTGAGAAGTATGAGTCGCTTGCTTCTGACCTTCTGGAGTGGATTGAACAAACCATCATCATTCTAAACAA CCGCAAATTTGCTAATTCACTGGTTGGGGTCCAGCAGCAGCTTCAGGCATTCAACACGTACCGCACAGTG GAGAAACCACCTAAGTTTACTGAGAAGGGGAATTTGGAGGTGCTGCTTTTCACAATTCAGAGCAAGATGA GAGCTAATAATCAGAAGGTCTACATGCCCCGAGAAGGGAAGCTCATCTCTGATATCAACAAGGCCTGGGA AAGACTGGAAAAAGCAGAACATGAGAGAGAACTGGCTCTGCGGAATGAGCTCATACGGCAGGAAAAACTG GAACAACTTGCCCGCAGATTCGATCGCAAGGCAGCTATGAGAGAGACATGGCTGAGCGAAAACCAGCGTC TTGTCTCTCAGGACAACTTTGGATTTGACCTTCCTGCAGTTGAGGCCGCCACCAAAAAACATGAGGCCAT CGAGACTGACATTGCGGCGTATGAAGAGCGAGTGCAGGCTGTGGTGGCTGTGGCCAGGGAGCTTGAAGCA GAGAGCTATCATGACATCAAGCGCATCACAGCGAGGAAGGACAATGTCATCCGCCTCTGGGAGTACTTGC TGGAACTGCTCAGGGCCAGGAGGCAGCGTCTTGAGATGAACCTGGGACTGCAAAAGATATTCCAGGAAAT GCTTTATATTATGGACTGGATGGATGAAATGAAGGTGCTATTGCTGTCTCAGGACTATGGCAAACACTTG CTCGGTGTTGAAGACTTGTTACAGAAGCATGCCCTGGTTGAAGCAGACATTGCAATCCAAGCAGAACGTG TGAGAGGTGTGAATGCCTCTGCCCAGAAGTTCGCAACAGATGGGGAAGGCTACAAGCCATGCGACCCCCA GGTAATCCGAGACCGTGTGGCCCACATGGAATTCTGCTATCAAGAACTCTGTCAGCTGGCAGCTGAGCGT AGGGCTCGCCTGGAAGAGTCTCGTCGCCTCTGGAAGTTCTTCTGGGAGATGGCGGAGGAGGAAGGCTGGA TACGGGAGAAGGAGAAGATCCTGTCCTCTGATGACTATGGGAAAGACCTGACTAGTGTCATGCGCCTGCT GAGCAAGCACAGGGCTTTTGAGGATGAGATGAGTGGCCGTAGTGGCCATTTTGAGCAGGCCATTAAAGAA GGTGAAGACATGATTGCAGAGGAACACTTTGGATCAGAAAAGATCCGTGAGAGAATCAGTTACATTCGAG AGCAGTGGGCCAACCTGGAGCAGCTCTCAGCCATTAGGAAGAAGCGCCTAGAGGAGGCCTCATTGCTGCA CCAGTTCCAGGCTGATGCTGATGACATCGACGCTTGGATGTTAGATATACTCAAGATTGTGTCCAGCAAC GATGTGGGCCATGATGAGTATTCCACACAGTCTCTGGTCAAGAAGCATAAAGATGTGGCAGAAGAGATCT CCAACTACAGACCCACTATTGACACACTGCATGAGCAAGCCGGTGCTCTTCCTCAAGCACACGCAGAGTC TCCAGATGTGAAGGGCCGGCTGGCAGGAATTGAGGAGCGTTACAAGGAGGTGGCAGAGTTAACACGGCTA AGGAAGCAGGCACTGCAGGACACCCTGGCTCTGTACAAGATGTTCAGTGAGGCTGATGCCTGTGAGCTCT GGATCGACGAGAAGGAGCAGTGGCTCAACAACATGCAGATCCCAGAGAAGCTGGAGGACCTGGAAGTCAT CCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAACCAGGCTTCCCGGGTTGCTGTGGTGAACCAG ATTGCACGGCAGCTGATGCACAGTGGCCATCCCAGCGAAAAGGAAATCAGAGCTCAGCAGGACAAACTCA ACACGAGGTGGAGTCAGTTCAGAGAACTGGTGGACAGGAAGAAGGATGCTCTTCTGTCCGCCCTGAGCAT CCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTGGATCCGGGAGAAGACCAAGGTCATCGAGTCT ACCCAAGACCTTGGCAATGACCTGGCAGGTGTCATGGCCCTGCAGCGCAAGCTGACCGGCATGGAACGAG ACTTGGTAGCCATCGAGGCGAAGCTGAGTGACCTGCAGAAGGAAGCCGAGAAGCTTGAGTCCGAGCACCC TGACCAGGCTCAAGCCATCCTGTCTCGACTGGCTGAGATCAGTGACGTGTGGGAGGAGATGAAGACAACC CTGAAGAACCGAGAAGCCTCGCTGGGAGAGGCCAGCAAGCTGCAGCAGTTCCTGCGGGACTTGGATGACT TCCAGTCTTGGCTCTCCAGGACCCAGACTGCTATTGCCTCAGAGGACATGCCCAATACCCTGACTGAGGC AGAGAAGCTTCTCACACAGCATGAGAACATCAAAAACGAGATTGACAATTACGAGGAAGACTACCAGAAG ATGCGGGACATGGGCGAGATGGTCACGCAGGGACAGACTGATGCCCAGTACATGTTTCTGCGGCAGCGGT TGCAGGCCTTGGACACAGGCTGGAACGAGCTCCACAAAATGTGGGAGAACAGGCAAAATCTCCTCTCCCA GTCCCATGCCTACCAGCAGTTCCTTAGGGACACGAAACAGGCTGAAGCTTTCCTTAATAACCAGGAGTAT GTTTTGGCTCATACTGAAATGCCCACCACCCTGGAAGGAGCTGAAGCAGCCATTAAGAAGCAGGAGGACT TCATGACCACCATGGATGCCAATGAGGAGAAGATCAATGCTGTTGTGGAGACTGGCCGGAGGCTGGTGAG CGATGGGAATATCAACTCCGACCGCATCCAGGAGAAGGTGGACTCTATTGATGACAGGCACAGAAAGAAT CGAGAAGCAGCCAGTGAACTTTTGATGAGGTTAAAGGACAACCGTGATCTACAGAAGTTCCTGCAAGATT GTCAAGAGCTGTCCCTCTGGATCAATGAAAAGATGCTTACAGCTCAGGACATGTCTTATGATGAAGCCAG AAACCTGCACAGTAAATGGTTAAAGCATCAAGCATTTATGGCAGAACTTGCATCCAACAAAGAATGGCTT GACAAAATTGAGAAGGAAGGAATGCAGCTTATTTCAGAAAAGCCAGAAACAGAGGCTGTGGTGAAGGAGA AACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAATCCACAACCCAGACCAAGGCCCAGAGGCTCTT CGATGCGAACAAGGCTGAGCTCTTCACGCAGAGCTGTGCAGACCTCGACAAATGGCTGCATGGCCTGGAG AGCCAGATTCAATCTGACGACTATGGCAAAGACCTCACCACTAGCGCCCAAAACAAAGTTCTAGATGGAG CCTCTGTGTTCCTTCTGATGTACTCTTGCCCCCCCAGATGCTGGAGAATCAGATGGAAGAGGGGAAGAGC ACAGATGAGGGTGGACAGCAAACGCCTCACTGTGCAGACCAAGTTCATGGAGCTTCTGGAGCCCTTGAAT GAAAGGAAGCATAATCTGCTGGCTTCCAAGGAGATCCACCAGTTCAACAGGGATGTAGAGGACGAGATTC TGTGGGTTGGCGAGAGGATGCCTTTGGCAACTTCCACAGATCATGGCCACAACCTTCAAACTGTGCAGCT GTTAATAAAGAAAAACCAGACCCTCCAGAAAGAAATCCAGGGACACCAGCCTCGTATTGATGACATCTTT GAGAGGAGCCAAAACATCATCACAGATAGCAGCAGCCTCAATGCCGAGGCTATCAGACAGAGGCTCGCCG ATCTGAAGCAGCTGTGGGGGCTCCTCATTGAGGAAACTGAGAAACGCCATAGACGGCTCGAGGAGGCACA CAAGGCCCAGCAATACTACTTTGATGCAGCTGAAGCAGAGGCATGGATGAGTGAACAGGAACTGTACATG ATGTCTGAGGAAAAGGCCAAGGATGAGCAGAGTGCTGTCTCCATGTTGAAAAAGCACCAGATTTTGGAAC AGGCTGTTGAGGACTATGCAGAGACAGTGCACCAGCTCTCCAAGACCAGCCGGGCACTGGTGGCTGACAG CCATCCCGAAAGTGAGCGTATTAGCATGCGGCAGTCAAAGGTCGACAAGCTGTATGCTGGCCTGAAGGAC CTTGCTGAGGAGAGGAGAGGGAAACTTGATGAGAGGCACAGGCTATTCCAGCTCAACAGGGAGGTAGATG ACCTGGAACAGTGGATCGCCGAGAGGGAAGTGGTTGCAGGCTCCCATGAGTTGGGACAGGACTATGAGCA TGTCACGATGTTACAAGAACGGTTCCGAGAATTTGCTCGAGACACAGGAAACATTGGGCAGGAGCGTGTA GATACAGTTAACCACATGGCAGATGACCTCATCAATTCTGGACATTCAGATGCTGCGACTATTGCTGAGT GGAAAGATGGTCTCAATGAAGCCTGGGCTGACCTCCTGGAGCTCATTGACACGAGAACACAGATTCTTGC TGCCTCTTACGAACTTCATAAGTTTTACCATGATGCCAAGGAGATCTTTGGCCGCATCCAGGACAAACAC AAGAAACTCCCTGAGGAGCTTGGAAGAGATCAGAACACCGTGGAGACTTTACAGAGAATGCACACCACCT TTGAACATGACATCCAAGCTCTGGGCACTCAGGTGAGGCAGCTGCAGGAGGATGCAGCCCGCCTCCAAGC AGCCTATGCAGGGGACAAGGCTGACGACATCCAAAAGCGTGAGAACGAGGTCCTGGAAGCCTGGAAGTCC CTGCTGGATGCTTGTGAGGGTCGCAGGGTGCGGCTAGTAGACACGGGAGACAAGTTCCGCTTCTTCAGCA TGGTGCGTGACCTCATGCTCTGGATGGAAGATGTCATCCGGCAAATCGAGGCCCAGGAGAAACCCCGGGA TGTGTCATCTGTTGAACTGTTAATGAATAATCATCAAGGCATCAAAGCTGAAATCGATGCTCGTAATGAC AGTTTTACAGCTTGCATTGAACTTGGGAAAGCCCTGCTGGCAAGGAAACACTATGCTTCTGAGGAGATCA AGGAAAAGTTACTGCAGTTGACGGAGAAAAGAAAAGAAATGATCGACAAGTGGGAAGACCGATGGGAGTG GTTAAGACTGATTTTGGAGGTCCATCAGTTCTCAAGAGATGCCAGTGTGGCTGAGGCTTGGCTGCTGGGA CAGGAGCCATACCTATCCAGCCGCGAAATCGGCCAGAGTGTGGATGAAGTGGAGAAGCTTATTAAGCGCC ATGAGGCATTTGAAAAATCTGCAGCGACCTGGGATGAGAGATTCTCTGCCCTGGAAAGGTTGACAACATT GGAGCTACTGGAAGTGCGCAGACAGCAAGAGGAAGAAGAAAGAAAGAGGCGACCACCGTCTCCGGAGCCA AGCGCGAAGGTTTCAGAGGAGGCTGAGTCCCAGCAATGGGACACTTCAAAAGGAGACCAAGTTTCCCAGA ATGGTTTGCCGGCTGAGCAGGGATCTCCACGGATGGCAGGAACCATGGAAACAAGTGAAATGGTCAACGG TGCTGCTGAGCAGAGGACAAGCTCCAAAGAATCCAGTCCTGTTCCTTCTCCCACCTCGGACCGCAAGGCC AAATCTGCACTTCCAGCCCAGAGTGCTGCCACCCTGCCAGCCAGGACCCTGGAGACGCCTGCGGCCCAGA TGGAAGGCTTCCTCAATCGGAAGCACGAGTGGGAGGCCCACAATAAGAAAGCCTCAAGCAGGTCCTGGCA CAATGTTTATTGTGTCATAAATAACCAAGAAATGGGCTTCTACAAAGATGCCAAGAGTGCTGCTTCTGGC GTCCCCTACCACAGTGAGGTCCCTGTGAGTTTGAAAGAGGCCATCTGCGAAGTGGCCCTTGATTACAAGA AGAAGAAGCATGTGTTCAAGCTAAGACTAAGTGATGGCAACGAGTACCTCTTCCAAGCCAAAGATGATGA TGAAATGAACACATGGATCCAGGCTATCACCTCTGCCATCTCCTCTGACAAGCACGACACGTCTGCCAGC ACCCAGAGTACGCCAGCATCCAGCCGAGCGCAGACCTTACCCACCAGCGTCGTCACCATCACCAGCGAGT CCAGTCCTGGCAAGAGGGAGAAGGACAAAGAGAAAGACAAAGAGAAGCGGTTCAGCCTTTTCGGCAAGAA GAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210353 representing NM_001013130 Red=Cloning site Green=Tags(s) MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLAR VSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVD GNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNA LIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKM KALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTV EKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKL EQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEA ESYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHL LGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAER RARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKE GEDMIAEEHFGSEKIRERISYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSN DVGHDEYSTQSLVKKHKDVAEEISNYRPTIDTLHEQAGALPQAHAESPDVKGRLAGIEERYKEVAELTRL RKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQ IARQLMHSGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIES TQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTT LKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQK MRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEY VLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKN REAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWL DKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLE SQIQSDDYGKDLTTSAQNKVLDGASVFLLMYSCPPRCWRIRWKRGRAQMRVDSKRLTVQTKFMELLEPLN ERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIF ERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYM MSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKD LAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERV DTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKH KKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKS LLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARND SFTACIELGKALLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLG QEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEP SAKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRMAGTMETSEMVNGAAEQRTSSKESSPVPSPTSDRKA KSALPAQSAATLPARTLETPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKSAASG VPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLSDGNEYLFQAKDDDEMNTWIQAITSAISSDKHDTSAS TQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001013130
ORF Size	7074 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001013130.1, NP_001013148.1
RefSeq Size	7332 bp
RefSeq ORF	7077 bp
Locus ID	305614
MW	273.6 kDa
Gene Summary	a short form of beta II spectrin that lacks a pleckstrin homology domain and has a different C-terminal sequence; subcellular localization differs from that of the long form [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.