Sptbn1 (NM_001013130) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Spnb1; Spnb2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210353 representing NM_001013130 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACCACGGTGGCCACAGACTATGACAACATTGAGATCCAGCAGCAGTACAGCGATGTGAACAACC GCTGGGACGTGGATGACTGGGACAATGAGAACAGCTCTGCACGGCTCTTTGAGCGCTCCCGCATCAAGGC CCTGGCAGATGAGCGTGAAGCTGTACAGAAGAAGACCTTCACCAAGTGGGTCAACTCCCACCTTGCCCGA GTGTCCTGCCGAATCACAGACCTGTACACTGACCTTCGAGATGGACGGATGCTCATCAAACTACTGGAGG TCCTCTCTGGAGAGAGGCTGCCTAAACCCACTAAGGGACGGATGCGCATCCACTGTCTGGAGAATGTCGA CAAGGCTCTTCAGTTCCTGAAGGAGCAGAGAGTCCATCTCGAGAACATGGGCTCCCATGATATTGTGGAT GGAAACCACCGGCTGACCCTCGGCCTCATCTGGACCATTATCCTGCGCTTCCAGATCCAGGATATCAGTG TGGAGACTGAAGATAACAAAGAGAAAAAGTCTGCTAAGGATGCATTGCTGCTGTGGTGCCAGATGAAGAC AGCTGGGTACCCTAATGTCAACATTCACAATTTCACCACTAGCTGGAGGGATGGCATGGCCTTCAATGCA CTGATACACAAACACCGGCCTGACCTGATAGATTTTGATAAACTGAAGAAATCGAATGCACACTACAATC TGCAGAATGCATTTAACCTGGCAGAGCAGCACCTTGGCCTTACTAAACTGTTAGACCCTGAAGATATCAG CGTGGACCACCCTGATGAGAAGTCCATCATCACGTATGTGGTCACTTACTACCACTACTTCTCCAAGATG AAGGCCTTGGCTGTCGAAGGAAAGCGCATTGGGAAGGTGCTTGATAATGCTATAGAAACAGAGAAAATGA TTGAGAAGTATGAGTCGCTTGCTTCTGACCTTCTGGAGTGGATTGAACAAACCATCATCATTCTAAACAA CCGCAAATTTGCTAATTCACTGGTTGGGGTCCAGCAGCAGCTTCAGGCATTCAACACGTACCGCACAGTG GAGAAACCACCTAAGTTTACTGAGAAGGGGAATTTGGAGGTGCTGCTTTTCACAATTCAGAGCAAGATGA GAGCTAATAATCAGAAGGTCTACATGCCCCGAGAAGGGAAGCTCATCTCTGATATCAACAAGGCCTGGGA AAGACTGGAAAAAGCAGAACATGAGAGAGAACTGGCTCTGCGGAATGAGCTCATACGGCAGGAAAAACTG GAACAACTTGCCCGCAGATTCGATCGCAAGGCAGCTATGAGAGAGACATGGCTGAGCGAAAACCAGCGTC TTGTCTCTCAGGACAACTTTGGATTTGACCTTCCTGCAGTTGAGGCCGCCACCAAAAAACATGAGGCCAT CGAGACTGACATTGCGGCGTATGAAGAGCGAGTGCAGGCTGTGGTGGCTGTGGCCAGGGAGCTTGAAGCA GAGAGCTATCATGACATCAAGCGCATCACAGCGAGGAAGGACAATGTCATCCGCCTCTGGGAGTACTTGC TGGAACTGCTCAGGGCCAGGAGGCAGCGTCTTGAGATGAACCTGGGACTGCAAAAGATATTCCAGGAAAT GCTTTATATTATGGACTGGATGGATGAAATGAAGGTGCTATTGCTGTCTCAGGACTATGGCAAACACTTG CTCGGTGTTGAAGACTTGTTACAGAAGCATGCCCTGGTTGAAGCAGACATTGCAATCCAAGCAGAACGTG TGAGAGGTGTGAATGCCTCTGCCCAGAAGTTCGCAACAGATGGGGAAGGCTACAAGCCATGCGACCCCCA GGTAATCCGAGACCGTGTGGCCCACATGGAATTCTGCTATCAAGAACTCTGTCAGCTGGCAGCTGAGCGT AGGGCTCGCCTGGAAGAGTCTCGTCGCCTCTGGAAGTTCTTCTGGGAGATGGCGGAGGAGGAAGGCTGGA TACGGGAGAAGGAGAAGATCCTGTCCTCTGATGACTATGGGAAAGACCTGACTAGTGTCATGCGCCTGCT GAGCAAGCACAGGGCTTTTGAGGATGAGATGAGTGGCCGTAGTGGCCATTTTGAGCAGGCCATTAAAGAA GGTGAAGACATGATTGCAGAGGAACACTTTGGATCAGAAAAGATCCGTGAGAGAATCAGTTACATTCGAG AGCAGTGGGCCAACCTGGAGCAGCTCTCAGCCATTAGGAAGAAGCGCCTAGAGGAGGCCTCATTGCTGCA CCAGTTCCAGGCTGATGCTGATGACATCGACGCTTGGATGTTAGATATACTCAAGATTGTGTCCAGCAAC GATGTGGGCCATGATGAGTATTCCACACAGTCTCTGGTCAAGAAGCATAAAGATGTGGCAGAAGAGATCT CCAACTACAGACCCACTATTGACACACTGCATGAGCAAGCCGGTGCTCTTCCTCAAGCACACGCAGAGTC TCCAGATGTGAAGGGCCGGCTGGCAGGAATTGAGGAGCGTTACAAGGAGGTGGCAGAGTTAACACGGCTA AGGAAGCAGGCACTGCAGGACACCCTGGCTCTGTACAAGATGTTCAGTGAGGCTGATGCCTGTGAGCTCT GGATCGACGAGAAGGAGCAGTGGCTCAACAACATGCAGATCCCAGAGAAGCTGGAGGACCTGGAAGTCAT CCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAACCAGGCTTCCCGGGTTGCTGTGGTGAACCAG ATTGCACGGCAGCTGATGCACAGTGGCCATCCCAGCGAAAAGGAAATCAGAGCTCAGCAGGACAAACTCA ACACGAGGTGGAGTCAGTTCAGAGAACTGGTGGACAGGAAGAAGGATGCTCTTCTGTCCGCCCTGAGCAT CCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTGGATCCGGGAGAAGACCAAGGTCATCGAGTCT ACCCAAGACCTTGGCAATGACCTGGCAGGTGTCATGGCCCTGCAGCGCAAGCTGACCGGCATGGAACGAG ACTTGGTAGCCATCGAGGCGAAGCTGAGTGACCTGCAGAAGGAAGCCGAGAAGCTTGAGTCCGAGCACCC TGACCAGGCTCAAGCCATCCTGTCTCGACTGGCTGAGATCAGTGACGTGTGGGAGGAGATGAAGACAACC CTGAAGAACCGAGAAGCCTCGCTGGGAGAGGCCAGCAAGCTGCAGCAGTTCCTGCGGGACTTGGATGACT TCCAGTCTTGGCTCTCCAGGACCCAGACTGCTATTGCCTCAGAGGACATGCCCAATACCCTGACTGAGGC AGAGAAGCTTCTCACACAGCATGAGAACATCAAAAACGAGATTGACAATTACGAGGAAGACTACCAGAAG ATGCGGGACATGGGCGAGATGGTCACGCAGGGACAGACTGATGCCCAGTACATGTTTCTGCGGCAGCGGT TGCAGGCCTTGGACACAGGCTGGAACGAGCTCCACAAAATGTGGGAGAACAGGCAAAATCTCCTCTCCCA GTCCCATGCCTACCAGCAGTTCCTTAGGGACACGAAACAGGCTGAAGCTTTCCTTAATAACCAGGAGTAT GTTTTGGCTCATACTGAAATGCCCACCACCCTGGAAGGAGCTGAAGCAGCCATTAAGAAGCAGGAGGACT TCATGACCACCATGGATGCCAATGAGGAGAAGATCAATGCTGTTGTGGAGACTGGCCGGAGGCTGGTGAG CGATGGGAATATCAACTCCGACCGCATCCAGGAGAAGGTGGACTCTATTGATGACAGGCACAGAAAGAAT CGAGAAGCAGCCAGTGAACTTTTGATGAGGTTAAAGGACAACCGTGATCTACAGAAGTTCCTGCAAGATT GTCAAGAGCTGTCCCTCTGGATCAATGAAAAGATGCTTACAGCTCAGGACATGTCTTATGATGAAGCCAG AAACCTGCACAGTAAATGGTTAAAGCATCAAGCATTTATGGCAGAACTTGCATCCAACAAAGAATGGCTT GACAAAATTGAGAAGGAAGGAATGCAGCTTATTTCAGAAAAGCCAGAAACAGAGGCTGTGGTGAAGGAGA AACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAATCCACAACCCAGACCAAGGCCCAGAGGCTCTT CGATGCGAACAAGGCTGAGCTCTTCACGCAGAGCTGTGCAGACCTCGACAAATGGCTGCATGGCCTGGAG AGCCAGATTCAATCTGACGACTATGGCAAAGACCTCACCACTAGCGCCCAAAACAAAGTTCTAGATGGAG CCTCTGTGTTCCTTCTGATGTACTCTTGCCCCCCCAGATGCTGGAGAATCAGATGGAAGAGGGGAAGAGC ACAGATGAGGGTGGACAGCAAACGCCTCACTGTGCAGACCAAGTTCATGGAGCTTCTGGAGCCCTTGAAT GAAAGGAAGCATAATCTGCTGGCTTCCAAGGAGATCCACCAGTTCAACAGGGATGTAGAGGACGAGATTC TGTGGGTTGGCGAGAGGATGCCTTTGGCAACTTCCACAGATCATGGCCACAACCTTCAAACTGTGCAGCT GTTAATAAAGAAAAACCAGACCCTCCAGAAAGAAATCCAGGGACACCAGCCTCGTATTGATGACATCTTT GAGAGGAGCCAAAACATCATCACAGATAGCAGCAGCCTCAATGCCGAGGCTATCAGACAGAGGCTCGCCG ATCTGAAGCAGCTGTGGGGGCTCCTCATTGAGGAAACTGAGAAACGCCATAGACGGCTCGAGGAGGCACA CAAGGCCCAGCAATACTACTTTGATGCAGCTGAAGCAGAGGCATGGATGAGTGAACAGGAACTGTACATG ATGTCTGAGGAAAAGGCCAAGGATGAGCAGAGTGCTGTCTCCATGTTGAAAAAGCACCAGATTTTGGAAC AGGCTGTTGAGGACTATGCAGAGACAGTGCACCAGCTCTCCAAGACCAGCCGGGCACTGGTGGCTGACAG CCATCCCGAAAGTGAGCGTATTAGCATGCGGCAGTCAAAGGTCGACAAGCTGTATGCTGGCCTGAAGGAC CTTGCTGAGGAGAGGAGAGGGAAACTTGATGAGAGGCACAGGCTATTCCAGCTCAACAGGGAGGTAGATG ACCTGGAACAGTGGATCGCCGAGAGGGAAGTGGTTGCAGGCTCCCATGAGTTGGGACAGGACTATGAGCA TGTCACGATGTTACAAGAACGGTTCCGAGAATTTGCTCGAGACACAGGAAACATTGGGCAGGAGCGTGTA GATACAGTTAACCACATGGCAGATGACCTCATCAATTCTGGACATTCAGATGCTGCGACTATTGCTGAGT GGAAAGATGGTCTCAATGAAGCCTGGGCTGACCTCCTGGAGCTCATTGACACGAGAACACAGATTCTTGC TGCCTCTTACGAACTTCATAAGTTTTACCATGATGCCAAGGAGATCTTTGGCCGCATCCAGGACAAACAC AAGAAACTCCCTGAGGAGCTTGGAAGAGATCAGAACACCGTGGAGACTTTACAGAGAATGCACACCACCT TTGAACATGACATCCAAGCTCTGGGCACTCAGGTGAGGCAGCTGCAGGAGGATGCAGCCCGCCTCCAAGC AGCCTATGCAGGGGACAAGGCTGACGACATCCAAAAGCGTGAGAACGAGGTCCTGGAAGCCTGGAAGTCC CTGCTGGATGCTTGTGAGGGTCGCAGGGTGCGGCTAGTAGACACGGGAGACAAGTTCCGCTTCTTCAGCA TGGTGCGTGACCTCATGCTCTGGATGGAAGATGTCATCCGGCAAATCGAGGCCCAGGAGAAACCCCGGGA TGTGTCATCTGTTGAACTGTTAATGAATAATCATCAAGGCATCAAAGCTGAAATCGATGCTCGTAATGAC AGTTTTACAGCTTGCATTGAACTTGGGAAAGCCCTGCTGGCAAGGAAACACTATGCTTCTGAGGAGATCA AGGAAAAGTTACTGCAGTTGACGGAGAAAAGAAAAGAAATGATCGACAAGTGGGAAGACCGATGGGAGTG GTTAAGACTGATTTTGGAGGTCCATCAGTTCTCAAGAGATGCCAGTGTGGCTGAGGCTTGGCTGCTGGGA CAGGAGCCATACCTATCCAGCCGCGAAATCGGCCAGAGTGTGGATGAAGTGGAGAAGCTTATTAAGCGCC ATGAGGCATTTGAAAAATCTGCAGCGACCTGGGATGAGAGATTCTCTGCCCTGGAAAGGTTGACAACATT GGAGCTACTGGAAGTGCGCAGACAGCAAGAGGAAGAAGAAAGAAAGAGGCGACCACCGTCTCCGGAGCCA AGCGCGAAGGTTTCAGAGGAGGCTGAGTCCCAGCAATGGGACACTTCAAAAGGAGACCAAGTTTCCCAGA ATGGTTTGCCGGCTGAGCAGGGATCTCCACGGATGGCAGGAACCATGGAAACAAGTGAAATGGTCAACGG TGCTGCTGAGCAGAGGACAAGCTCCAAAGAATCCAGTCCTGTTCCTTCTCCCACCTCGGACCGCAAGGCC AAATCTGCACTTCCAGCCCAGAGTGCTGCCACCCTGCCAGCCAGGACCCTGGAGACGCCTGCGGCCCAGA TGGAAGGCTTCCTCAATCGGAAGCACGAGTGGGAGGCCCACAATAAGAAAGCCTCAAGCAGGTCCTGGCA CAATGTTTATTGTGTCATAAATAACCAAGAAATGGGCTTCTACAAAGATGCCAAGAGTGCTGCTTCTGGC GTCCCCTACCACAGTGAGGTCCCTGTGAGTTTGAAAGAGGCCATCTGCGAAGTGGCCCTTGATTACAAGA AGAAGAAGCATGTGTTCAAGCTAAGACTAAGTGATGGCAACGAGTACCTCTTCCAAGCCAAAGATGATGA TGAAATGAACACATGGATCCAGGCTATCACCTCTGCCATCTCCTCTGACAAGCACGACACGTCTGCCAGC ACCCAGAGTACGCCAGCATCCAGCCGAGCGCAGACCTTACCCACCAGCGTCGTCACCATCACCAGCGAGT CCAGTCCTGGCAAGAGGGAGAAGGACAAAGAGAAAGACAAAGAGAAGCGGTTCAGCCTTTTCGGCAAGAA GAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001013130
Insert Size	7077 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001013130.1, NP_001013148.1
RefSeq Size	7332 bp
RefSeq ORF	7077 bp
Locus ID	305614
Gene Summary	a short form of beta II spectrin that lacks a pleckstrin homology domain and has a different C-terminal sequence; subcellular localization differs from that of the long form [RGD, Feb 2006]

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