Unc13a (NM_022861) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Munc13-1; Unc13h1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210110 representing NM_022861 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCTGTGCGTGGGAGTCAAAAAAGCCAAGTTTGACGGTGCCCAAGAGAAGTTCAACACATACG TGACGCTGAAGGTGCAGAACGTGAAGAGCACTACCATAGCTGTACGCGGCAGCCAGCCTAGCTGGGAGCA GGACTTCATGTTTGAGATCAACCGCCTGGATCTGGGCCTGACGGTGGAGGTGTGGAACAAGGGTCTCATC TGGGACACAATGGTGGGTACTGTGTGGATCCCACTTCGGACCATCCGTCAGTCCAATGAGGAGGGTCCGG GAGAATGGCTGACACTGGACTCTCAGGCCATCATGGCGGACAGTGAGATCTGTGGGACCAAGGACCCCAC CTTCCACCGCATCCTCCTGGACGCACATTTTGAGCTGCCTTTGGACATCCCCGAGGAGGAGGCGCGCTAC TGGGCCAAGAAGCTGGAGCAGCTGAATGCCATGCGTGACCAAGATGAGTACTCCTTTCAGGACCAGCAGG ACAAGCCACTGCCGGTGCCCAGCAGCCAGTGCTGCAACTGGAATTACTTTGGCTGGGGAGAACAGAATGA TGATCCCGACAGTGCCGTGGATGACCGGGACAGTGATTATAGGAGTGAGACGAGCAACAGCATCCCACCG CCTTACTACACGACTTCGCAGCCCAATGCTTCGGTGCACCAGTACTCCGTGCGGCCACCCCCTCTGGGGT CCCGGGAGTCCTACAGCGACTCCATGCACAGCTATGAAGAGTTCTCTGAGCCGCGGGCACTCAGTCCCAC AGGCAGCAGCCGCTATGCTTCCAGTGGGGAGCTGAGCCAGGGCAGCTCCCAGCTGAGTGAGGACTTCGAC CCGGATGAGCACAGCCTACAGGGCTCAGAGCTGGATGACGAGAGGGACCGAGATTCTTATCACTCCTGCC ACAGCTCTGTGAGCTACCACAAGGACTCACCCCGCTGGGACCAGGATGAGGAGGACCTGGAGGACCTGGA GGACCTGGAAGATGAGGAGTTGCCCGAGGAAGAGGAGTTGGAAGAGGAGGAGTTGGAGGAGGAGGAGGAG TTGGAGGAAGAGGAATTGGAGTTGGAGGAGGAGGAGGAGGTGCCTGATGACCTGGCCAGCTATACCCAGC AGGAGGACACCACTGTGGCTGAGCCCAAAGAGTTCAAGCGGATCAGCTTCCCAACAGCTGCACCTCAGAA GGAAGACAAAGTTTCAGCTGTGCCCATTGAGGCCCCCGATGTGTCCAAAGGCATCCCCAAGGCAGCCACA CCTGAAGAGAAGGCAGCTGCGGAGTGTGCACAGGAAGCGGAGCCCCCCAAGTCTGAGGAGAGTTTCAGAT CTCGAGAGGCTGAGGAGGGCCAGGAAGGGCAGGATGCCATGTCCAGGGCCAAAGCCAACTGGTTGCGAGC CTTCAACAAGGTGCGCATGCAGCTGCAGGAGGCCCGAGGAGAAGGAGAGATGTCCAAGTCTCTGTGGTTC AAAGGCGGCCCTGGTGGTGGCCTTATCATCATTGACAGCATGCCAGACATCAGGAAGCGGAAGCCCATTC CCCTCGTGAGCGACCTGGCTATGTCTCTGGTCCAGTCACGGAAGGCGGGCATCACCTCGGCCTTGGCCTC CAGCACGTTGAACAATGAAGAGTTGAAAAACCACGTTTACAAGAAGACCCTGCAAGCCTTAATCTACCCC ATCTCCTGCACCACGCCGCACAACTTCGAGGTGTGGACGGCCACCACTCCCACCTACTGCTACGAGTGCG AGGGGCTGCTGTGGGGCATCGCGCGGCAGGGCATGCGCTGCACCGAGTGCGGCGTTAAGTGCCACGAGAA GTGCCAAGACCTGCTCAACGCGGACTGCCTGCAGCGGGCGGCTGAGAAGAGTTCTAAGCATGGCGCTGAA GACCGCACGCAGAACATCATCATGGTGCTGAAGGACCGCATGAAGATCCGCGAGCGCAACAAGCCTGAGA TCTTCGAGCTGATCCAGGAGGTCTTCGCGGTCACCAAGAGCGCACACACACAGCAGATGAAGGCCGTCAA GCAGAGTGTGCTGGATGGCACATCCAAGTGGTCTGCCAAAATTAGCATCACGGTGGTCTGTGCCCAGGGC TTGCAGGCCAAGGACAAGACAGGATCCAGTGACCCTTATGTCACCGTCCAGGTTGGGAAGACCAAGAAAA GGACAAAAACCATCTACGGGAACCTTAACCCAGTGTGGGAAGAGAATTTCCACTTTGAATGTCACAACTC CTCTGACCGGATCAAGGTGCGTGTGTTGGATGAAGACGATGACATCAAATCCCGTGTGAAGCAAAGGTTT AAGAGGGAGTCTGATGACTTCCTAGGGCAGACAATCATCGAGGTGCGGACGCTTAGCGGCGAGATGGATG TGTGGTACAATCTGGACAAGAGAACAGACAAGTCTGCTGTGTCGGGCGCCATTCGGCTTCACATCAGTGT GGAGATCAAAGGGGAGGAGAAGGTGGCACCCTACCATGTCCAGTACACCTGTCTGCATGAGAACCTGTTC CATTTTGTGACGGATGTACAGAACAATGGCGTGGTGAAGATTCCCGATGCCAAAGGTGACGACGCCTGGA AGGTTTACTATGATGAGACTGCCCAGGAGATCGTGGATGAGTTTGCCATGCGCTATGGCGTTGAATCCAT CTACCAAGCCATGACCCACTTTGCCTGCCTCTCCTCCAAGTACATGTGCCCTGGGGTACCCGCTGTCATG AGCACCCTGCTTGCCAACATCAACGCCTACTACGCACACACCACCGCCTCCACCAACGTGTCTGCCTCCG ACCGCTTCGCTGCCTCCAATTTCGGGAAAGAGCGCTTTGTGAAACTTCTGGACCAGCTGCACAACTCCCT GCGGATTGACCTGTCCATGTACCGGAACAACTTCCCGGCCAGCAGCCCCGAGCGGCTGCAGGATCTCAAG TCCACAGTGGACCTGCTTACCAGCATCACCTTCTTCCGGATGAAGGTTCAGGAACTGCAGAGTCCGCCAC GTGCCAGTCAGGTGGTGAAGGACTGCGTGAAGGCGTGCCTCAACTCCACCTACGAGTACATCTTCAACAA CTGTCATGAGCTCTATGGCCGGGAGTACCAGACAGACCCGGCCAAGAAGGGGGAGGTCCCCCCGGAGGAG CAAGGCCCTAGCATCAAGAACCTGGATTTCTGGTCCAAGCTCATCACCCTCATCGTGTCTATCATTGAGG AGGATAAGAACTCCTACACACCCTGCCTCAATCAGTTTCCCCAGGAGCTGAATGTGGGGAAGATCAGTGC TGAGGTGATGTGGAGCCTGTTTGCCCAGGACATGAAGTACGCCATGGAGGAACACGACAAGCACCGGCTG TGTAAGAGCGCAGACTACATGAACCTGCATTTCAAGGTCAAGTGGTTGTACAACGAGTACGTGGCTGAAC TGCCCACCTTCAAGGACCGCGTGCCGGAGTACCCTGCGTGGTTTGAGCCCTTCGTCATCCAGTGGTTAGA TGAGAATGAGGAGGTGTCCCGGGACTTCCTGCATGGTGCACTCGAGCGGGACAAGAAGGATGGGTTCCAG CAGACATCGGAGCACGCCCTGTTCTCTTGCTCGGTAGTGGACGTCTTCTCCCAGCTCAACCAGAGCTTTG AGATCATCAAGAAGCTGGAGTGTCCTGACCCCCAGATTGTGGGCCACTACATGCGGCGCTTTGCCAAGAC CATTAGCAATGTGCTTCTCCAGTACGCCGACATCGTCTCCAAGGACTTCGCTTCCTACTGCTCCAAGGAG AAGGAGAAAGTGCCCTGCATCCTCATGAACAACACACAGCAGCTGCGGGTGCAGCTGGAGAAGATGTTCG AGGCGATGGGTGGGAAGGAGCTGGACGCCGAGGCCAGCGGAACCCTGAAGGAGCTGCAGGTGAAACTCAA CAATGTCCTGGATGAACTCAGCCACGTGTTTGCCACCAGCTTCCAGCCACACATCGAGGAGTGTGTCAGA CAAATGGGTGACATCCTAAGCCAAGTGAAGGGCACGGGCAACGTGCCCGCCAGTGCCTGCAGCAGCGTGG CACAGGACGCAGACAACGTGCTACAGCCCATCATGGATCTTCTGGACAGCAACCTCACCCTGTTTGCCAA AATCTGTGAGAAGACGGTTCTGAAGCGGGTGCTGAAGGAGCTGTGGAAGCTGGTGATGAACACCATGGAG AGGACCATTGTCCTGCCGCCACTCACTGACCAGACGATGATTGGTACCCTCTTGAGAAAACATGGCAAGG GCCTAGAAAAGGGCAGGGTGAAACTGCCAAGCCACTCAGACGGGACACAAATGATCTTCAATGCCGCCAA GGAGCTGGGCCAGCTGTCCAAACTGAAGGATCACATGGTGCGAGAAGAAGCCAAGAGCTTGACCCCGAAG CAGTGTGCCGTGGTTGAACTGGCCCTGGACACCATCAAGCAATACTTCCACGCGGGGGGCGTGGGCCTCA AGAAGACCTTTCTCGAGAAAAGCCCGGACCTTCAGTCCCTGCGCTACGCCCTGTCGCTCTACACACAGGC CACCGACCTGCTCATCAAAACCTTCGTGCAGACGCAGTCAGCGCAGGTCCATGGTGGAAAGGGGACTAGG TTTACCCTTAGTGAAGACGTTTGTCCTGAGATGGGCTCGGGTGTGGAAGACCCTGTAGGTGAAGTATCCG TCCACGTGGAGCTGTTCACGCATCCGGGAACTGGGGAACAGAAGGTCACAGTGAAGGTGGTGGCCGCCAA CGACCTCAAGTGGCAGACTTCTGGCATCTTCCGTCCGTTCATTGAGGTCAACATCGTTGGACCTCAGCTC AGCGACAAGAAACGAAAGTTCGCCACCAAATCGAAAAACAACAGCTGGGCGCCCAAATATAACGAGAGCT TCCAGTTCTCCCTGAGCGCCGACGCGGGACCCGAGTGCTACGAGTTGCAGGTGTGCGTGAAGGACTACTG CTTCGCGCGCGAGGACCGCACGGTGGAGCTGGCGGTGCTGCAGCTGCGGGAGCTGGCTCAGCGCGGGAGC GCCGCGTGCTGGCTGCCGCTCGGCCGCCGCATCCACATGGACGACACGGGGCTCACAGTGCTGCGTATCC TGTCGCAGCGCAGCAATGACGAGGTGGCCAAGGAGTTCGTCAAGCTCAAGTCCGACACGCGCTCGGCCGA GGAGGGCGGTGCCGCGCCTGCGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210110 representing NM_022861 Red=Cloning site Green=Tags(s) MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLI WDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARY WAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPP PYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFD PDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDEEDLEDLEDLEDEELPEEEELEEEELEEEEE LEEEELELEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKEDKVSAVPIEAPDVSKGIPKAAT PEEKAAAECAQEAEPPKSEESFRSREAEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGEMSKSLWF KGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYP ISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAE DRTQNIIMVLKDRMKIRERNKPEIFELIQEVFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQG LQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVLDEDDDIKSRVKQRF KRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLF HFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVM STLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLK STVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEE QGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRL CKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQ QTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKE KEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVR QMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTME RTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPK QCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTR FTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQL SDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVELAVLQLRELAQRGS AACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022861
ORF Size	5205 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022861.1, NP_074052.1
RefSeq Size	6683 bp
RefSeq ORF	5208 bp
Locus ID	64829
UniProt ID	Q62768
MW	196.4 kDa
Gene Summary	brain specific protein that may have role during neurotransmitter release [RGD, Feb 2006]

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