Unc13a (NM_022861) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Munc13-1; Unc13h1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210110 representing NM_022861 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCTGTGCGTGGGAGTCAAAAAAGCCAAGTTTGACGGTGCCCAAGAGAAGTTCAACACATACG TGACGCTGAAGGTGCAGAACGTGAAGAGCACTACCATAGCTGTACGCGGCAGCCAGCCTAGCTGGGAGCA GGACTTCATGTTTGAGATCAACCGCCTGGATCTGGGCCTGACGGTGGAGGTGTGGAACAAGGGTCTCATC TGGGACACAATGGTGGGTACTGTGTGGATCCCACTTCGGACCATCCGTCAGTCCAATGAGGAGGGTCCGG GAGAATGGCTGACACTGGACTCTCAGGCCATCATGGCGGACAGTGAGATCTGTGGGACCAAGGACCCCAC CTTCCACCGCATCCTCCTGGACGCACATTTTGAGCTGCCTTTGGACATCCCCGAGGAGGAGGCGCGCTAC TGGGCCAAGAAGCTGGAGCAGCTGAATGCCATGCGTGACCAAGATGAGTACTCCTTTCAGGACCAGCAGG ACAAGCCACTGCCGGTGCCCAGCAGCCAGTGCTGCAACTGGAATTACTTTGGCTGGGGAGAACAGAATGA TGATCCCGACAGTGCCGTGGATGACCGGGACAGTGATTATAGGAGTGAGACGAGCAACAGCATCCCACCG CCTTACTACACGACTTCGCAGCCCAATGCTTCGGTGCACCAGTACTCCGTGCGGCCACCCCCTCTGGGGT CCCGGGAGTCCTACAGCGACTCCATGCACAGCTATGAAGAGTTCTCTGAGCCGCGGGCACTCAGTCCCAC AGGCAGCAGCCGCTATGCTTCCAGTGGGGAGCTGAGCCAGGGCAGCTCCCAGCTGAGTGAGGACTTCGAC CCGGATGAGCACAGCCTACAGGGCTCAGAGCTGGATGACGAGAGGGACCGAGATTCTTATCACTCCTGCC ACAGCTCTGTGAGCTACCACAAGGACTCACCCCGCTGGGACCAGGATGAGGAGGACCTGGAGGACCTGGA GGACCTGGAAGATGAGGAGTTGCCCGAGGAAGAGGAGTTGGAAGAGGAGGAGTTGGAGGAGGAGGAGGAG TTGGAGGAAGAGGAATTGGAGTTGGAGGAGGAGGAGGAGGTGCCTGATGACCTGGCCAGCTATACCCAGC AGGAGGACACCACTGTGGCTGAGCCCAAAGAGTTCAAGCGGATCAGCTTCCCAACAGCTGCACCTCAGAA GGAAGACAAAGTTTCAGCTGTGCCCATTGAGGCCCCCGATGTGTCCAAAGGCATCCCCAAGGCAGCCACA CCTGAAGAGAAGGCAGCTGCGGAGTGTGCACAGGAAGCGGAGCCCCCCAAGTCTGAGGAGAGTTTCAGAT CTCGAGAGGCTGAGGAGGGCCAGGAAGGGCAGGATGCCATGTCCAGGGCCAAAGCCAACTGGTTGCGAGC CTTCAACAAGGTGCGCATGCAGCTGCAGGAGGCCCGAGGAGAAGGAGAGATGTCCAAGTCTCTGTGGTTC AAAGGCGGCCCTGGTGGTGGCCTTATCATCATTGACAGCATGCCAGACATCAGGAAGCGGAAGCCCATTC CCCTCGTGAGCGACCTGGCTATGTCTCTGGTCCAGTCACGGAAGGCGGGCATCACCTCGGCCTTGGCCTC CAGCACGTTGAACAATGAAGAGTTGAAAAACCACGTTTACAAGAAGACCCTGCAAGCCTTAATCTACCCC ATCTCCTGCACCACGCCGCACAACTTCGAGGTGTGGACGGCCACCACTCCCACCTACTGCTACGAGTGCG AGGGGCTGCTGTGGGGCATCGCGCGGCAGGGCATGCGCTGCACCGAGTGCGGCGTTAAGTGCCACGAGAA GTGCCAAGACCTGCTCAACGCGGACTGCCTGCAGCGGGCGGCTGAGAAGAGTTCTAAGCATGGCGCTGAA GACCGCACGCAGAACATCATCATGGTGCTGAAGGACCGCATGAAGATCCGCGAGCGCAACAAGCCTGAGA TCTTCGAGCTGATCCAGGAGGTCTTCGCGGTCACCAAGAGCGCACACACACAGCAGATGAAGGCCGTCAA GCAGAGTGTGCTGGATGGCACATCCAAGTGGTCTGCCAAAATTAGCATCACGGTGGTCTGTGCCCAGGGC TTGCAGGCCAAGGACAAGACAGGATCCAGTGACCCTTATGTCACCGTCCAGGTTGGGAAGACCAAGAAAA GGACAAAAACCATCTACGGGAACCTTAACCCAGTGTGGGAAGAGAATTTCCACTTTGAATGTCACAACTC CTCTGACCGGATCAAGGTGCGTGTGTTGGATGAAGACGATGACATCAAATCCCGTGTGAAGCAAAGGTTT AAGAGGGAGTCTGATGACTTCCTAGGGCAGACAATCATCGAGGTGCGGACGCTTAGCGGCGAGATGGATG TGTGGTACAATCTGGACAAGAGAACAGACAAGTCTGCTGTGTCGGGCGCCATTCGGCTTCACATCAGTGT GGAGATCAAAGGGGAGGAGAAGGTGGCACCCTACCATGTCCAGTACACCTGTCTGCATGAGAACCTGTTC CATTTTGTGACGGATGTACAGAACAATGGCGTGGTGAAGATTCCCGATGCCAAAGGTGACGACGCCTGGA AGGTTTACTATGATGAGACTGCCCAGGAGATCGTGGATGAGTTTGCCATGCGCTATGGCGTTGAATCCAT CTACCAAGCCATGACCCACTTTGCCTGCCTCTCCTCCAAGTACATGTGCCCTGGGGTACCCGCTGTCATG AGCACCCTGCTTGCCAACATCAACGCCTACTACGCACACACCACCGCCTCCACCAACGTGTCTGCCTCCG ACCGCTTCGCTGCCTCCAATTTCGGGAAAGAGCGCTTTGTGAAACTTCTGGACCAGCTGCACAACTCCCT GCGGATTGACCTGTCCATGTACCGGAACAACTTCCCGGCCAGCAGCCCCGAGCGGCTGCAGGATCTCAAG TCCACAGTGGACCTGCTTACCAGCATCACCTTCTTCCGGATGAAGGTTCAGGAACTGCAGAGTCCGCCAC GTGCCAGTCAGGTGGTGAAGGACTGCGTGAAGGCGTGCCTCAACTCCACCTACGAGTACATCTTCAACAA CTGTCATGAGCTCTATGGCCGGGAGTACCAGACAGACCCGGCCAAGAAGGGGGAGGTCCCCCCGGAGGAG CAAGGCCCTAGCATCAAGAACCTGGATTTCTGGTCCAAGCTCATCACCCTCATCGTGTCTATCATTGAGG AGGATAAGAACTCCTACACACCCTGCCTCAATCAGTTTCCCCAGGAGCTGAATGTGGGGAAGATCAGTGC TGAGGTGATGTGGAGCCTGTTTGCCCAGGACATGAAGTACGCCATGGAGGAACACGACAAGCACCGGCTG TGTAAGAGCGCAGACTACATGAACCTGCATTTCAAGGTCAAGTGGTTGTACAACGAGTACGTGGCTGAAC TGCCCACCTTCAAGGACCGCGTGCCGGAGTACCCTGCGTGGTTTGAGCCCTTCGTCATCCAGTGGTTAGA TGAGAATGAGGAGGTGTCCCGGGACTTCCTGCATGGTGCACTCGAGCGGGACAAGAAGGATGGGTTCCAG CAGACATCGGAGCACGCCCTGTTCTCTTGCTCGGTAGTGGACGTCTTCTCCCAGCTCAACCAGAGCTTTG AGATCATCAAGAAGCTGGAGTGTCCTGACCCCCAGATTGTGGGCCACTACATGCGGCGCTTTGCCAAGAC CATTAGCAATGTGCTTCTCCAGTACGCCGACATCGTCTCCAAGGACTTCGCTTCCTACTGCTCCAAGGAG AAGGAGAAAGTGCCCTGCATCCTCATGAACAACACACAGCAGCTGCGGGTGCAGCTGGAGAAGATGTTCG AGGCGATGGGTGGGAAGGAGCTGGACGCCGAGGCCAGCGGAACCCTGAAGGAGCTGCAGGTGAAACTCAA CAATGTCCTGGATGAACTCAGCCACGTGTTTGCCACCAGCTTCCAGCCACACATCGAGGAGTGTGTCAGA CAAATGGGTGACATCCTAAGCCAAGTGAAGGGCACGGGCAACGTGCCCGCCAGTGCCTGCAGCAGCGTGG CACAGGACGCAGACAACGTGCTACAGCCCATCATGGATCTTCTGGACAGCAACCTCACCCTGTTTGCCAA AATCTGTGAGAAGACGGTTCTGAAGCGGGTGCTGAAGGAGCTGTGGAAGCTGGTGATGAACACCATGGAG AGGACCATTGTCCTGCCGCCACTCACTGACCAGACGATGATTGGTACCCTCTTGAGAAAACATGGCAAGG GCCTAGAAAAGGGCAGGGTGAAACTGCCAAGCCACTCAGACGGGACACAAATGATCTTCAATGCCGCCAA GGAGCTGGGCCAGCTGTCCAAACTGAAGGATCACATGGTGCGAGAAGAAGCCAAGAGCTTGACCCCGAAG CAGTGTGCCGTGGTTGAACTGGCCCTGGACACCATCAAGCAATACTTCCACGCGGGGGGCGTGGGCCTCA AGAAGACCTTTCTCGAGAAAAGCCCGGACCTTCAGTCCCTGCGCTACGCCCTGTCGCTCTACACACAGGC CACCGACCTGCTCATCAAAACCTTCGTGCAGACGCAGTCAGCGCAGGTCCATGGTGGAAAGGGGACTAGG TTTACCCTTAGTGAAGACGTTTGTCCTGAGATGGGCTCGGGTGTGGAAGACCCTGTAGGTGAAGTATCCG TCCACGTGGAGCTGTTCACGCATCCGGGAACTGGGGAACAGAAGGTCACAGTGAAGGTGGTGGCCGCCAA CGACCTCAAGTGGCAGACTTCTGGCATCTTCCGTCCGTTCATTGAGGTCAACATCGTTGGACCTCAGCTC AGCGACAAGAAACGAAAGTTCGCCACCAAATCGAAAAACAACAGCTGGGCGCCCAAATATAACGAGAGCT TCCAGTTCTCCCTGAGCGCCGACGCGGGACCCGAGTGCTACGAGTTGCAGGTGTGCGTGAAGGACTACTG CTTCGCGCGCGAGGACCGCACGGTGGAGCTGGCGGTGCTGCAGCTGCGGGAGCTGGCTCAGCGCGGGAGC GCCGCGTGCTGGCTGCCGCTCGGCCGCCGCATCCACATGGACGACACGGGGCTCACAGTGCTGCGTATCC TGTCGCAGCGCAGCAATGACGAGGTGGCCAAGGAGTTCGTCAAGCTCAAGTCCGACACGCGCTCGGCCGA GGAGGGCGGTGCCGCGCCTGCGCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022861
Insert Size	5208 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022861.1, NP_074052.1
RefSeq Size	6683 bp
RefSeq ORF	5208 bp
Locus ID	64829
UniProt ID	Q62768
Gene Summary	brain specific protein that may have role during neurotransmitter release [RGD, Feb 2006]

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